Assembly of DNA-functionalized gold nanoparticles studied by UV/Vis-spectroscopy and dynamic light scattering
The DNA mediated assembly of complementary DNA-functionalized gold nanoparticles (DNA-AuNP) was investigated by means of UV/Vis-spectroscopy and Dynamic Light Scattering (DLS). The melting temperature of the aggregates was determined to be T(m) = 31 degrees C. Characterization of the assembly at 20...
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| Veröffentlicht in: | Physical chemistry chemical physics : PCCP 2008-01, Vol.10 (14), p.1870-1875 |
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| container_end_page | 1875 |
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| container_issue | 14 |
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| container_title | Physical chemistry chemical physics : PCCP |
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| creator | Witten, Katrin G Bretschneider, Jan C Eckert, Thomas Richtering, Walter Simon, Ulrich |
| description | The DNA mediated assembly of complementary DNA-functionalized gold nanoparticles (DNA-AuNP) was investigated by means of UV/Vis-spectroscopy and Dynamic Light Scattering (DLS). The melting temperature of the aggregates was determined to be T(m) = 31 degrees C. Characterization of the assembly at 20 degrees C, 25 degrees C and 30 degrees C showed a decrease of the initial assembly growth rate with increasing temperature. The correlation of the wavelengths at the absorbance maxima lambda(max) and the hydrodynamic radii R(h) of the AuNP assemblies proved the dependence of the optical properties on the assembly size while at higher assembly temperature (30 degrees C) a larger redshift of lambda(max) with increasing R(h) was observed than at lower temperatures. This tendency might give information about the dependence of the internal structure of the DNA-AuNP assemblies on assembly temperature. It is assumed that at higher temperatures more compact assemblies are built than at lower temperatures of 20 degrees C and 25 degrees C. To the best of our knowledge, this is the first systematic time-resolved in situ investigation of DNA-mediated AuNP assembly by UV/Vis-spectroscopy and DLS. |
| doi_str_mv | 10.1039/b719762d |
| format | Article |
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The melting temperature of the aggregates was determined to be T(m) = 31 degrees C. Characterization of the assembly at 20 degrees C, 25 degrees C and 30 degrees C showed a decrease of the initial assembly growth rate with increasing temperature. The correlation of the wavelengths at the absorbance maxima lambda(max) and the hydrodynamic radii R(h) of the AuNP assemblies proved the dependence of the optical properties on the assembly size while at higher assembly temperature (30 degrees C) a larger redshift of lambda(max) with increasing R(h) was observed than at lower temperatures. This tendency might give information about the dependence of the internal structure of the DNA-AuNP assemblies on assembly temperature. It is assumed that at higher temperatures more compact assemblies are built than at lower temperatures of 20 degrees C and 25 degrees C. To the best of our knowledge, this is the first systematic time-resolved in situ investigation of DNA-mediated AuNP assembly by UV/Vis-spectroscopy and DLS.</description><identifier>ISSN: 1463-9076</identifier><identifier>EISSN: 1463-9084</identifier><identifier>DOI: 10.1039/b719762d</identifier><identifier>PMID: 18368179</identifier><language>eng</language><publisher>England</publisher><subject>DNA - chemistry ; Gold - chemistry ; Image Processing, Computer-Assisted ; Metal Nanoparticles - chemistry ; Microscopy, Electron, Scanning Transmission ; Scattering, Radiation ; Spectrophotometry, Ultraviolet ; Temperature ; Time Factors</subject><ispartof>Physical chemistry chemical physics : PCCP, 2008-01, Vol.10 (14), p.1870-1875</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c347t-2bf185c5910e7833c5588fc4f98d7c86cf3de1a04e671102317d819294b41bfe3</citedby><cites>FETCH-LOGICAL-c347t-2bf185c5910e7833c5588fc4f98d7c86cf3de1a04e671102317d819294b41bfe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18368179$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Witten, Katrin G</creatorcontrib><creatorcontrib>Bretschneider, Jan C</creatorcontrib><creatorcontrib>Eckert, Thomas</creatorcontrib><creatorcontrib>Richtering, Walter</creatorcontrib><creatorcontrib>Simon, Ulrich</creatorcontrib><title>Assembly of DNA-functionalized gold nanoparticles studied by UV/Vis-spectroscopy and dynamic light scattering</title><title>Physical chemistry chemical physics : PCCP</title><addtitle>Phys Chem Chem Phys</addtitle><description>The DNA mediated assembly of complementary DNA-functionalized gold nanoparticles (DNA-AuNP) was investigated by means of UV/Vis-spectroscopy and Dynamic Light Scattering (DLS). The melting temperature of the aggregates was determined to be T(m) = 31 degrees C. Characterization of the assembly at 20 degrees C, 25 degrees C and 30 degrees C showed a decrease of the initial assembly growth rate with increasing temperature. The correlation of the wavelengths at the absorbance maxima lambda(max) and the hydrodynamic radii R(h) of the AuNP assemblies proved the dependence of the optical properties on the assembly size while at higher assembly temperature (30 degrees C) a larger redshift of lambda(max) with increasing R(h) was observed than at lower temperatures. This tendency might give information about the dependence of the internal structure of the DNA-AuNP assemblies on assembly temperature. It is assumed that at higher temperatures more compact assemblies are built than at lower temperatures of 20 degrees C and 25 degrees C. To the best of our knowledge, this is the first systematic time-resolved in situ investigation of DNA-mediated AuNP assembly by UV/Vis-spectroscopy and DLS.</description><subject>DNA - chemistry</subject><subject>Gold - chemistry</subject><subject>Image Processing, Computer-Assisted</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Microscopy, Electron, Scanning Transmission</subject><subject>Scattering, Radiation</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>1463-9076</issn><issn>1463-9084</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLAzEUhYMotlbBXyBZiZuxuU1mklmW-oSiG9vtkMmjRjIPJ5nF-OsdadXVPXA_DocPoUsgt0BoPi855Dxb6CM0BZbRJCeCHf9lnk3QWQgfhBBIgZ6iCQiaCeD5FFXLEExV-gE3Ft-9LBPb1yq6ppbefRmNd43XuJZ108ouOuVNwCH22o2vcsCb7XzrQhJao2LXBNW0A5a1xnqoZeUU9m73HnFQMkbTuXp3jk6s9MFcHO4MbR7u31ZPyfr18Xm1XCeKMh6TRWlBpCrNgRguKFVpKoRVzOZCcyUyZak2IAkzGQcgCwpcC8gXOSsZlNbQGbre97Zd89mbEIvKBWW8l7Vp-lBwwigXQozgzR5U4_zQGVu0natkNxRAih-1xa_aEb06dPZlZfQ_eHBJvwEcN3TR</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Witten, Katrin G</creator><creator>Bretschneider, Jan C</creator><creator>Eckert, Thomas</creator><creator>Richtering, Walter</creator><creator>Simon, Ulrich</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080101</creationdate><title>Assembly of DNA-functionalized gold nanoparticles studied by UV/Vis-spectroscopy and dynamic light scattering</title><author>Witten, Katrin G ; Bretschneider, Jan C ; Eckert, Thomas ; Richtering, Walter ; Simon, Ulrich</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c347t-2bf185c5910e7833c5588fc4f98d7c86cf3de1a04e671102317d819294b41bfe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>DNA - chemistry</topic><topic>Gold - chemistry</topic><topic>Image Processing, Computer-Assisted</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Microscopy, Electron, Scanning Transmission</topic><topic>Scattering, Radiation</topic><topic>Spectrophotometry, Ultraviolet</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Witten, Katrin G</creatorcontrib><creatorcontrib>Bretschneider, Jan C</creatorcontrib><creatorcontrib>Eckert, Thomas</creatorcontrib><creatorcontrib>Richtering, Walter</creatorcontrib><creatorcontrib>Simon, Ulrich</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Physical chemistry chemical physics : PCCP</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Witten, Katrin G</au><au>Bretschneider, Jan C</au><au>Eckert, Thomas</au><au>Richtering, Walter</au><au>Simon, Ulrich</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Assembly of DNA-functionalized gold nanoparticles studied by UV/Vis-spectroscopy and dynamic light scattering</atitle><jtitle>Physical chemistry chemical physics : PCCP</jtitle><addtitle>Phys Chem Chem Phys</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>10</volume><issue>14</issue><spage>1870</spage><epage>1875</epage><pages>1870-1875</pages><issn>1463-9076</issn><eissn>1463-9084</eissn><abstract>The DNA mediated assembly of complementary DNA-functionalized gold nanoparticles (DNA-AuNP) was investigated by means of UV/Vis-spectroscopy and Dynamic Light Scattering (DLS). The melting temperature of the aggregates was determined to be T(m) = 31 degrees C. Characterization of the assembly at 20 degrees C, 25 degrees C and 30 degrees C showed a decrease of the initial assembly growth rate with increasing temperature. The correlation of the wavelengths at the absorbance maxima lambda(max) and the hydrodynamic radii R(h) of the AuNP assemblies proved the dependence of the optical properties on the assembly size while at higher assembly temperature (30 degrees C) a larger redshift of lambda(max) with increasing R(h) was observed than at lower temperatures. This tendency might give information about the dependence of the internal structure of the DNA-AuNP assemblies on assembly temperature. It is assumed that at higher temperatures more compact assemblies are built than at lower temperatures of 20 degrees C and 25 degrees C. To the best of our knowledge, this is the first systematic time-resolved in situ investigation of DNA-mediated AuNP assembly by UV/Vis-spectroscopy and DLS.</abstract><cop>England</cop><pmid>18368179</pmid><doi>10.1039/b719762d</doi><tpages>6</tpages></addata></record> |
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| ispartof | Physical chemistry chemical physics : PCCP, 2008-01, Vol.10 (14), p.1870-1875 |
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| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | DNA - chemistry Gold - chemistry Image Processing, Computer-Assisted Metal Nanoparticles - chemistry Microscopy, Electron, Scanning Transmission Scattering, Radiation Spectrophotometry, Ultraviolet Temperature Time Factors |
| title | Assembly of DNA-functionalized gold nanoparticles studied by UV/Vis-spectroscopy and dynamic light scattering |
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