Peptide profile of human acquired enamel pellicle using MALDI tandem MS

The present study proposes a strategy for human in vivo acquired enamel pellicle (AEP) peptidome characterisation based on sequential extraction with guanidine and TFA followed by MALDI‐TOF/TOF identification. Three different nanoscale analytical approaches were used: samples were subjected to trypt...

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Veröffentlicht in:	Journal of separation science 2008-02, Vol.31 (3), p.523-537
Hauptverfasser:	Vitorino, Rui, Calheiros-Lobo, Maria João, Duarte, José A., Domingues, Pedro M., Amado, Francisco M.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chromatography, High Pressure Liquid - methods Dental Pellicle - chemistry Humans LC-MS/MS Molecular Sequence Data Peptide Fragments - analysis Peptide Fragments - classification Proteolytic activity Reproducibility of Results Salivary peptides Sensitivity and Specificity Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods Tandem Mass Spectrometry - methods Whole saliva
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