Conjugation of a Water-Soluble Gadolinium Endohedral Fulleride with an Antibody as a Magnetic Resonance Imaging Contrast Agent
Water-soluble gadofullerides exhibited high efficiency as magnetic resonance imaging (MRI) contrast agents. In this paper, we report the conjugation of the newly synthesized gadofulleride, Gd@C82O6(OH)16(NHCH2CH2COOH)8, with the antibody of green fluorescence protein (anti-GFP), as a model for “tumo...
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| Veröffentlicht in: | Bioconjugate chemistry 2008-03, Vol.19 (3), p.651-655 |
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| creator | Shu, Chun-Ying Ma, Xin-Yong Zhang, Jian-Fei Corwin, Frank D Sim, Jae Hyun Zhang, Er-Yun Dorn, Harry C Gibson, Harry W Fatouros, Panos P Wang, Chun-Ru Fang, Xiao-Hong |
| description | Water-soluble gadofullerides exhibited high efficiency as magnetic resonance imaging (MRI) contrast agents. In this paper, we report the conjugation of the newly synthesized gadofulleride, Gd@C82O6(OH)16(NHCH2CH2COOH)8, with the antibody of green fluorescence protein (anti-GFP), as a model for “tumor targeted” imaging agents based on endohedral metallofullerenes. In this model system, the activity of the anti-GFP conjugate can be conveniently detected by green fluorescence protein (GFP), leading to in vitro experiments more direct and facile than those of tumor antibodies. Objective-type total internal reflection fluorescence microscopy revealed that each gadofulleride aggregate conjugated on average five anti-GFPs, and the activity of anti-GFPs was preserved after conjugation. In addition, the gadofulleride/antibody conjugate exhibited higher water proton relaxivity (12.0 mM−1 s−1) than the parent gadofulleride aggregate (8.1 mM−1 s−1) in phosphate buffered saline at 0.35 T, as also confirmed by T 1-weighted images of phantoms. These observations clearly indicate that the synthesized gadofulleride/antibody conjugate not only has targeting potential, but also exhibits higher efficiency as an MRI contrast agent. |
| doi_str_mv | 10.1021/bc7002742 |
| format | Article |
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In this paper, we report the conjugation of the newly synthesized gadofulleride, Gd@C82O6(OH)16(NHCH2CH2COOH)8, with the antibody of green fluorescence protein (anti-GFP), as a model for “tumor targeted” imaging agents based on endohedral metallofullerenes. In this model system, the activity of the anti-GFP conjugate can be conveniently detected by green fluorescence protein (GFP), leading to in vitro experiments more direct and facile than those of tumor antibodies. Objective-type total internal reflection fluorescence microscopy revealed that each gadofulleride aggregate conjugated on average five anti-GFPs, and the activity of anti-GFPs was preserved after conjugation. In addition, the gadofulleride/antibody conjugate exhibited higher water proton relaxivity (12.0 mM−1 s−1) than the parent gadofulleride aggregate (8.1 mM−1 s−1) in phosphate buffered saline at 0.35 T, as also confirmed by T 1-weighted images of phantoms. These observations clearly indicate that the synthesized gadofulleride/antibody conjugate not only has targeting potential, but also exhibits higher efficiency as an MRI contrast agent.</description><identifier>ISSN: 1043-1802</identifier><identifier>EISSN: 1520-4812</identifier><identifier>DOI: 10.1021/bc7002742</identifier><identifier>PMID: 18254583</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Antibodies - chemistry ; Biochemistry ; Biotin - chemistry ; Chemical Phenomena ; Chemistry, Physical ; Contrast Media - chemical synthesis ; Fluorescence ; Gadolinium - chemistry ; Green Fluorescent Proteins - immunology ; Immune system ; Indicators and Reagents ; Magnetic Resonance Imaging - methods ; Molecular Conformation ; Molecular Weight ; NMR ; Nuclear magnetic resonance ; Proteins ; Serum Albumin, Bovine - chemistry ; Solubility ; Spectrometry, Fluorescence ; Streptavidin - chemistry ; Water</subject><ispartof>Bioconjugate chemistry, 2008-03, Vol.19 (3), p.651-655</ispartof><rights>Copyright © 2008 American Chemical Society</rights><rights>Copyright American Chemical Society Mar 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a378t-4b14a0a9e976fd57a510a0a8f1ae17354d99011646d081fe22f75a89da6f7b923</citedby><cites>FETCH-LOGICAL-a378t-4b14a0a9e976fd57a510a0a8f1ae17354d99011646d081fe22f75a89da6f7b923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/bc7002742$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/bc7002742$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18254583$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shu, Chun-Ying</creatorcontrib><creatorcontrib>Ma, Xin-Yong</creatorcontrib><creatorcontrib>Zhang, Jian-Fei</creatorcontrib><creatorcontrib>Corwin, Frank D</creatorcontrib><creatorcontrib>Sim, Jae Hyun</creatorcontrib><creatorcontrib>Zhang, Er-Yun</creatorcontrib><creatorcontrib>Dorn, Harry C</creatorcontrib><creatorcontrib>Gibson, Harry W</creatorcontrib><creatorcontrib>Fatouros, Panos P</creatorcontrib><creatorcontrib>Wang, Chun-Ru</creatorcontrib><creatorcontrib>Fang, Xiao-Hong</creatorcontrib><title>Conjugation of a Water-Soluble Gadolinium Endohedral Fulleride with an Antibody as a Magnetic Resonance Imaging Contrast Agent</title><title>Bioconjugate chemistry</title><addtitle>Bioconjugate Chem</addtitle><description>Water-soluble gadofullerides exhibited high efficiency as magnetic resonance imaging (MRI) contrast agents. In this paper, we report the conjugation of the newly synthesized gadofulleride, Gd@C82O6(OH)16(NHCH2CH2COOH)8, with the antibody of green fluorescence protein (anti-GFP), as a model for “tumor targeted” imaging agents based on endohedral metallofullerenes. In this model system, the activity of the anti-GFP conjugate can be conveniently detected by green fluorescence protein (GFP), leading to in vitro experiments more direct and facile than those of tumor antibodies. Objective-type total internal reflection fluorescence microscopy revealed that each gadofulleride aggregate conjugated on average five anti-GFPs, and the activity of anti-GFPs was preserved after conjugation. In addition, the gadofulleride/antibody conjugate exhibited higher water proton relaxivity (12.0 mM−1 s−1) than the parent gadofulleride aggregate (8.1 mM−1 s−1) in phosphate buffered saline at 0.35 T, as also confirmed by T 1-weighted images of phantoms. These observations clearly indicate that the synthesized gadofulleride/antibody conjugate not only has targeting potential, but also exhibits higher efficiency as an MRI contrast agent.</description><subject>Antibodies - chemistry</subject><subject>Biochemistry</subject><subject>Biotin - chemistry</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Contrast Media - chemical synthesis</subject><subject>Fluorescence</subject><subject>Gadolinium - chemistry</subject><subject>Green Fluorescent Proteins - immunology</subject><subject>Immune system</subject><subject>Indicators and Reagents</subject><subject>Magnetic Resonance Imaging - methods</subject><subject>Molecular Conformation</subject><subject>Molecular Weight</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Proteins</subject><subject>Serum Albumin, Bovine - chemistry</subject><subject>Solubility</subject><subject>Spectrometry, Fluorescence</subject><subject>Streptavidin - chemistry</subject><subject>Water</subject><issn>1043-1802</issn><issn>1520-4812</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0VFrFDEQB_BFLLZWH_wCEgQFH1aTbLLZfTyP9ixULL2K4EuY3cxuc-4lbZJF--JnN-WOFvQlyZAf_xmYonjF6AdGOfvY9YpSrgR_UhwxyWkpGsaf5jcVVckayg-L5zFuKKUta_iz4jCfUsimOir-LL3bzCMk6x3xAwHyHRKGcu2nuZuQrMD4yTo7b8mJM_4aTYCJnM7ThMEaJL9suibgyMIl23lzRyDmjC8wOky2J5cYvQPXIznbwmjdSHK_FCAmshjRpRfFwQBTxJf7-7j4dnpytfxcnn9dnS0X5yVUqkml6JgACi22qh6MVCAZzXUzMECmKilM21LGalEb2rABOR-UhKY1UA-qa3l1XLzb5d4EfztjTHprY4_TBA79HLWignNJ6wzf_AM3fg4uz6Y5q1nbClll9H6H-uBjDDjom2C3EO40o_p-I_phI9m-3gfO3RbNo9yvIINyB2xM-PvhH8JPXatKSX11sdbqx8Xqslov9afs3-489PFxuP8b_wX32qAy</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Shu, Chun-Ying</creator><creator>Ma, Xin-Yong</creator><creator>Zhang, Jian-Fei</creator><creator>Corwin, Frank D</creator><creator>Sim, Jae Hyun</creator><creator>Zhang, Er-Yun</creator><creator>Dorn, Harry C</creator><creator>Gibson, Harry W</creator><creator>Fatouros, Panos P</creator><creator>Wang, Chun-Ru</creator><creator>Fang, Xiao-Hong</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20080301</creationdate><title>Conjugation of a Water-Soluble Gadolinium Endohedral Fulleride with an Antibody as a Magnetic Resonance Imaging Contrast Agent</title><author>Shu, Chun-Ying ; Ma, Xin-Yong ; Zhang, Jian-Fei ; Corwin, Frank D ; Sim, Jae Hyun ; Zhang, Er-Yun ; Dorn, Harry C ; Gibson, Harry W ; Fatouros, Panos P ; Wang, Chun-Ru ; Fang, Xiao-Hong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a378t-4b14a0a9e976fd57a510a0a8f1ae17354d99011646d081fe22f75a89da6f7b923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Antibodies - chemistry</topic><topic>Biochemistry</topic><topic>Biotin - chemistry</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Contrast Media - chemical synthesis</topic><topic>Fluorescence</topic><topic>Gadolinium - chemistry</topic><topic>Green Fluorescent Proteins - immunology</topic><topic>Immune system</topic><topic>Indicators and Reagents</topic><topic>Magnetic Resonance Imaging - methods</topic><topic>Molecular Conformation</topic><topic>Molecular Weight</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Proteins</topic><topic>Serum Albumin, Bovine - chemistry</topic><topic>Solubility</topic><topic>Spectrometry, Fluorescence</topic><topic>Streptavidin - chemistry</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shu, Chun-Ying</creatorcontrib><creatorcontrib>Ma, Xin-Yong</creatorcontrib><creatorcontrib>Zhang, Jian-Fei</creatorcontrib><creatorcontrib>Corwin, Frank D</creatorcontrib><creatorcontrib>Sim, Jae Hyun</creatorcontrib><creatorcontrib>Zhang, Er-Yun</creatorcontrib><creatorcontrib>Dorn, Harry C</creatorcontrib><creatorcontrib>Gibson, Harry W</creatorcontrib><creatorcontrib>Fatouros, Panos P</creatorcontrib><creatorcontrib>Wang, Chun-Ru</creatorcontrib><creatorcontrib>Fang, Xiao-Hong</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioconjugate chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shu, Chun-Ying</au><au>Ma, Xin-Yong</au><au>Zhang, Jian-Fei</au><au>Corwin, Frank D</au><au>Sim, Jae Hyun</au><au>Zhang, Er-Yun</au><au>Dorn, Harry C</au><au>Gibson, Harry W</au><au>Fatouros, Panos P</au><au>Wang, Chun-Ru</au><au>Fang, Xiao-Hong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conjugation of a Water-Soluble Gadolinium Endohedral Fulleride with an Antibody as a Magnetic Resonance Imaging Contrast Agent</atitle><jtitle>Bioconjugate chemistry</jtitle><addtitle>Bioconjugate Chem</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>19</volume><issue>3</issue><spage>651</spage><epage>655</epage><pages>651-655</pages><issn>1043-1802</issn><eissn>1520-4812</eissn><abstract>Water-soluble gadofullerides exhibited high efficiency as magnetic resonance imaging (MRI) contrast agents. In this paper, we report the conjugation of the newly synthesized gadofulleride, Gd@C82O6(OH)16(NHCH2CH2COOH)8, with the antibody of green fluorescence protein (anti-GFP), as a model for “tumor targeted” imaging agents based on endohedral metallofullerenes. In this model system, the activity of the anti-GFP conjugate can be conveniently detected by green fluorescence protein (GFP), leading to in vitro experiments more direct and facile than those of tumor antibodies. Objective-type total internal reflection fluorescence microscopy revealed that each gadofulleride aggregate conjugated on average five anti-GFPs, and the activity of anti-GFPs was preserved after conjugation. In addition, the gadofulleride/antibody conjugate exhibited higher water proton relaxivity (12.0 mM−1 s−1) than the parent gadofulleride aggregate (8.1 mM−1 s−1) in phosphate buffered saline at 0.35 T, as also confirmed by T 1-weighted images of phantoms. These observations clearly indicate that the synthesized gadofulleride/antibody conjugate not only has targeting potential, but also exhibits higher efficiency as an MRI contrast agent.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>18254583</pmid><doi>10.1021/bc7002742</doi><tpages>5</tpages></addata></record> |
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| ispartof | Bioconjugate chemistry, 2008-03, Vol.19 (3), p.651-655 |
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| subjects | Antibodies - chemistry Biochemistry Biotin - chemistry Chemical Phenomena Chemistry, Physical Contrast Media - chemical synthesis Fluorescence Gadolinium - chemistry Green Fluorescent Proteins - immunology Immune system Indicators and Reagents Magnetic Resonance Imaging - methods Molecular Conformation Molecular Weight NMR Nuclear magnetic resonance Proteins Serum Albumin, Bovine - chemistry Solubility Spectrometry, Fluorescence Streptavidin - chemistry Water |
| title | Conjugation of a Water-Soluble Gadolinium Endohedral Fulleride with an Antibody as a Magnetic Resonance Imaging Contrast Agent |
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