Vibrio cholerae non-O1, non-O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes
In this study, we report the presence of the SXT element and Class I integron in Vibrio cholerae non-O1, non-O139 strains isolated from Varanasi, India. Isolates were resistant to cotrimoxazole, trimethoprim and/or streptomycin, furazolidone and ampicillin. None contained plasmids. Polymerase chain...
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| Veröffentlicht in: | Environmental microbiology 2008-04, Vol.10 (4), p.866-873 |
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| creator | Mohapatra, Harapriya Mohapatra, Saswat S Mantri, Chinmay K Colwell, Rita R Singh, Durg V |
| description | In this study, we report the presence of the SXT element and Class I integron in Vibrio cholerae non-O1, non-O139 strains isolated from Varanasi, India. Isolates were resistant to cotrimoxazole, trimethoprim and/or streptomycin, furazolidone and ampicillin. None contained plasmids. Polymerase chain reaction (PCR) and DNA sequencing revealed the presence of antibiotic resistance gene cassettes, aadA1, aadA2, aadA5 and dfrA15, in the Class I integron and SXT, an integrative conjugative element containing dfr18, sulII and strAB, in three and six of the isolates respectively. Conjugation experiments, followed by PCR analysis of transconjugants, provided evidence for the transferable nature of intSXT and associated antibiotic resistance gene cassettes. This is the first report of the occurrence of SXT ICE, dfr18, sulII, strAB and aadA5 genes in environmental V. cholerae non-O1, non-O139 strains from Varanasi, India, that had been isolated before 1992. |
| doi_str_mv | 10.1111/j.1462-2920.2007.01502.x |
| format | Article |
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Isolates were resistant to cotrimoxazole, trimethoprim and/or streptomycin, furazolidone and ampicillin. None contained plasmids. Polymerase chain reaction (PCR) and DNA sequencing revealed the presence of antibiotic resistance gene cassettes, aadA1, aadA2, aadA5 and dfrA15, in the Class I integron and SXT, an integrative conjugative element containing dfr18, sulII and strAB, in three and six of the isolates respectively. Conjugation experiments, followed by PCR analysis of transconjugants, provided evidence for the transferable nature of intSXT and associated antibiotic resistance gene cassettes. This is the first report of the occurrence of SXT ICE, dfr18, sulII, strAB and aadA5 genes in environmental V. cholerae non-O1, non-O139 strains from Varanasi, India, that had been isolated before 1992.</description><subject>Ampicillin - pharmacology</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacterial Proteins - genetics</subject><subject>Drug Resistance, Multiple, Bacterial - genetics</subject><subject>Folic Acid Antagonists - pharmacology</subject><subject>Furazolidone - pharmacology</subject><subject>Humans</subject><subject>India</subject><subject>Integrases - genetics</subject><subject>Integrons - genetics</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Streptomycin - pharmacology</subject><subject>Trimethoprim - pharmacology</subject><subject>Trimethoprim, Sulfamethoxazole Drug Combination - pharmacology</subject><subject>Vibrio cholerae non-O1 - drug effects</subject><subject>Vibrio cholerae non-O1 - genetics</subject><subject>Vibrio cholerae non-O1 - isolation & purification</subject><subject>Vibrio Infections - microbiology</subject><subject>Water Microbiology</subject><issn>1462-2912</issn><issn>1462-2920</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkc9u1DAQhyMEoqXwCuATp00Y20kTHziUqrQrSnvY_uFmTWJ78ZK1FzsR2wfhfXHYZfFlRvp9M7LmyzJCoaDpfVgVtDxlORMMCgZQF0ArYMX2WXZ8CJ4fesqOslcxrgBozWt4mR3RhgGlojnOfj_YNlhPuu--1wE1cd7lt3S2r1yQOAS0LhIbfY-DVqTVxgdNqBCMmODX5AEDOox2RuZOWSSY0vXYD3bTa6LCuCRBRxsHdMOMdN4NaR-xblh8u5sRZQJtCDpFENVZRZba6fg6e2Gwj_rNvp5k958v7s6v8uvby_n52XVuOKtYzrEyhlPWVjWWwLVpQFHBoQPRCNF2Za0MLWsDFcNGgKHGtNy0ddtApRhofpK93-3dBP9z1HGQaxs73ffotB-jrKFktKx4At_uwbFdayU3wa4xPMl_h0zAxx3wy_b66X8OchImV3JyIScvchIm_wqTW3nxdT51aT7fzac76e1hHsMPeZqkVfLx5lLecHpVfll8ko-Jf7fjDXqJy2CjvF-kv3CApi4F5_wPjYifqQ</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Mohapatra, Harapriya</creator><creator>Mohapatra, Saswat S</creator><creator>Mantri, Chinmay K</creator><creator>Colwell, Rita R</creator><creator>Singh, Durg V</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>Vibrio cholerae non-O1, non-O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes</title><author>Mohapatra, Harapriya ; Mohapatra, Saswat S ; Mantri, Chinmay K ; Colwell, Rita R ; Singh, Durg V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f3252-3a5ff312b57a403ef80d1930c09899bc47df147f052a890f1ffb3fb7b805d20e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Ampicillin - pharmacology</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacterial Proteins - genetics</topic><topic>Drug Resistance, Multiple, Bacterial - genetics</topic><topic>Folic Acid Antagonists - pharmacology</topic><topic>Furazolidone - pharmacology</topic><topic>Humans</topic><topic>India</topic><topic>Integrases - genetics</topic><topic>Integrons - genetics</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Streptomycin - pharmacology</topic><topic>Trimethoprim - pharmacology</topic><topic>Trimethoprim, Sulfamethoxazole Drug Combination - pharmacology</topic><topic>Vibrio cholerae non-O1 - drug effects</topic><topic>Vibrio cholerae non-O1 - genetics</topic><topic>Vibrio cholerae non-O1 - isolation & purification</topic><topic>Vibrio Infections - microbiology</topic><topic>Water Microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohapatra, Harapriya</creatorcontrib><creatorcontrib>Mohapatra, Saswat S</creatorcontrib><creatorcontrib>Mantri, Chinmay K</creatorcontrib><creatorcontrib>Colwell, Rita R</creatorcontrib><creatorcontrib>Singh, Durg V</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohapatra, Harapriya</au><au>Mohapatra, Saswat S</au><au>Mantri, Chinmay K</au><au>Colwell, Rita R</au><au>Singh, Durg V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Vibrio cholerae non-O1, non-O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes</atitle><jtitle>Environmental microbiology</jtitle><addtitle>Environ Microbiol</addtitle><date>2008-04</date><risdate>2008</risdate><volume>10</volume><issue>4</issue><spage>866</spage><epage>873</epage><pages>866-873</pages><issn>1462-2912</issn><eissn>1462-2920</eissn><abstract>In this study, we report the presence of the SXT element and Class I integron in Vibrio cholerae non-O1, non-O139 strains isolated from Varanasi, India. Isolates were resistant to cotrimoxazole, trimethoprim and/or streptomycin, furazolidone and ampicillin. None contained plasmids. Polymerase chain reaction (PCR) and DNA sequencing revealed the presence of antibiotic resistance gene cassettes, aadA1, aadA2, aadA5 and dfrA15, in the Class I integron and SXT, an integrative conjugative element containing dfr18, sulII and strAB, in three and six of the isolates respectively. Conjugation experiments, followed by PCR analysis of transconjugants, provided evidence for the transferable nature of intSXT and associated antibiotic resistance gene cassettes. This is the first report of the occurrence of SXT ICE, dfr18, sulII, strAB and aadA5 genes in environmental V. cholerae non-O1, non-O139 strains from Varanasi, India, that had been isolated before 1992.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18201198</pmid><doi>10.1111/j.1462-2920.2007.01502.x</doi><tpages>8</tpages></addata></record> |
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| subjects | Ampicillin - pharmacology Anti-Bacterial Agents - pharmacology Bacterial Proteins - genetics Drug Resistance, Multiple, Bacterial - genetics Folic Acid Antagonists - pharmacology Furazolidone - pharmacology Humans India Integrases - genetics Integrons - genetics Microbial Sensitivity Tests Molecular Sequence Data Multigene Family Streptomycin - pharmacology Trimethoprim - pharmacology Trimethoprim, Sulfamethoxazole Drug Combination - pharmacology Vibrio cholerae non-O1 - drug effects Vibrio cholerae non-O1 - genetics Vibrio cholerae non-O1 - isolation & purification Vibrio Infections - microbiology Water Microbiology |
| title | Vibrio cholerae non-O1, non-O139 strains isolated before 1992 from Varanasi, India are multiple drug resistant, contain intSXT, dfr18 and aadA5 genes |
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