Complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1
Pineapple mealybug wilt-associated virus-1 (PMWaV-1; family Closteroviridae, genus Ampelovirus) belongs to a complex of mealybug-transmissible viruses found in pineapple worldwide. In this study, the complete genome of PMWaV-1 was sequenced and found to be 13.1 kb in length, making it the smallest i...
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Veröffentlicht in: | Archives of virology 2008-04, Vol.153 (4), p.707-714 |
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description | Pineapple mealybug wilt-associated virus-1 (PMWaV-1; family Closteroviridae, genus Ampelovirus) belongs to a complex of mealybug-transmissible viruses found in pineapple worldwide. In this study, the complete genome of PMWaV-1 was sequenced and found to be 13.1 kb in length, making it the smallest in the family. The genome encoded seven open reading frames (ORFs) and was unusual for an ampelovirus due to the lack of an intergenic region between the RdRp and p6 ORFs, an ORF encoding a relatively small coat protein (CP), and the absence of an ORF encoding a coat protein duplicate (CPd). Phylogenetic analyses placed PMWaV-1, plum bark necrosis stem pitting-associated virus and some grapevine leafroll-associated viruses in a distinct clade within the genus Ampelovirus. |
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Phylogenetic analyses placed PMWaV-1, plum bark necrosis stem pitting-associated virus and some grapevine leafroll-associated viruses in a distinct clade within the genus Ampelovirus.</description><identifier>ISSN: 0304-8608</identifier><identifier>EISSN: 1432-8798</identifier><identifier>DOI: 10.1007/s00705-008-0051-8</identifier><identifier>PMID: 18283409</identifier><language>eng</language><publisher>Vienna: Vienna : Springer Vienna</publisher><subject>Amino Acid Sequence ; Amino acids ; Ananas - virology ; Animals ; Biomedical and Life Sciences ; Biomedicine ; Cloning ; Closteroviridae - classification ; Closteroviridae - genetics ; Genome, Viral ; Genomes ; Hemiptera - virology ; Infectious Diseases ; Medical Microbiology ; Molecular Sequence Data ; Open Reading Frames ; Original Article ; Phylogenetics ; Phylogeny ; Proteins ; Prunus ; RNA polymerase ; Sequence Alignment ; Sequence Analysis, DNA ; Virology ; Viruses ; Vitaceae</subject><ispartof>Archives of virology, 2008-04, Vol.153 (4), p.707-714</ispartof><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-3a6c10db956d35fdd10c9767caa46ba49963544e38e7d91867163df2bf064f9f3</citedby><cites>FETCH-LOGICAL-c455t-3a6c10db956d35fdd10c9767caa46ba49963544e38e7d91867163df2bf064f9f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00705-008-0051-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00705-008-0051-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18283409$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Melzer, M. 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The genome encoded seven open reading frames (ORFs) and was unusual for an ampelovirus due to the lack of an intergenic region between the RdRp and p6 ORFs, an ORF encoding a relatively small coat protein (CP), and the absence of an ORF encoding a coat protein duplicate (CPd). Phylogenetic analyses placed PMWaV-1, plum bark necrosis stem pitting-associated virus and some grapevine leafroll-associated viruses in a distinct clade within the genus Ampelovirus.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Ananas - virology</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cloning</subject><subject>Closteroviridae - classification</subject><subject>Closteroviridae - genetics</subject><subject>Genome, Viral</subject><subject>Genomes</subject><subject>Hemiptera - virology</subject><subject>Infectious Diseases</subject><subject>Medical Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Open Reading Frames</subject><subject>Original Article</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Proteins</subject><subject>Prunus</subject><subject>RNA polymerase</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Virology</subject><subject>Viruses</subject><subject>Vitaceae</subject><issn>0304-8608</issn><issn>1432-8798</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkU-P1SAUxYnROM-nH8CNEhfuqpcCLSzNi_-SSVzorAkttw2TFiq0mvHTy0tfMokLZwEk8Dvncu8h5CWDdwygfZ_LBrICUGVJVqlH5MAEryvVavWYHICDqFQD6oo8y_kWoFxw-ZRcMVUrLkAfSHeK8zLhijRs_YRx9Q5pxp8bhh6pDY6OGOKMNKbRBv_Hrj4GGge6-IB2KVI6o53uum2kv_20Vjbn2Hu7oqO_fNpyxZ6TJ4OdMr64nEdy8-njj9OX6vrb56-nD9dVL6RcK26bnoHrtGwcl4NzDHrdNm1vrWg6K7RuuBQCucLWaaaaljXcDXU3QCMGPfAjebv7LimW_-fVzD73OE02YNyyaUGw0rV-EKxBlyGWQT4EMq1AtbIu4Jt_wNu4pVC6NTWrectkmfeRsB3qU8w54WCW5Geb7gwDc87T7Hmakqc552nOmlcX462b0d0rLgEWoN6BXJ7CiOm-8v9cX--iwUZjx-SzufleA-OFaZUQwP8CCuizzw</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Melzer, M. 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subjects | Amino Acid Sequence Amino acids Ananas - virology Animals Biomedical and Life Sciences Biomedicine Cloning Closteroviridae - classification Closteroviridae - genetics Genome, Viral Genomes Hemiptera - virology Infectious Diseases Medical Microbiology Molecular Sequence Data Open Reading Frames Original Article Phylogenetics Phylogeny Proteins Prunus RNA polymerase Sequence Alignment Sequence Analysis, DNA Virology Viruses Vitaceae |
title | Complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1 |
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