Production of xylanase by immobilized Trichoderma reesei SAF3 in Ca-alginate beads
In the present study, the optimum conditions for the production of xylanase by immobilized spores of Trichoderma reesei SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous fermentation was also studied. The influence...
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| Veröffentlicht in: | Journal of industrial microbiology & biotechnology 2008-04, Vol.35 (4), p.245-249 |
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| container_title | Journal of industrial microbiology & biotechnology |
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| creator | Kar, Sanjay Mandal, Asish Mohapatra, Pradeep K. Das Samanta, Saptadip Pati, Bikash R. Mondal, Keshab C. |
| description | In the present study, the optimum conditions for the production of xylanase by immobilized spores of
Trichoderma reesei
SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous fermentation was also studied. The influence of alginate concentration (1, 2, 3, and 4%) and initial cell loading (100, 200, 300, 400, and 500 beads per flask) on xylanase production was considered. The production of xylanase was found to increase significantly with increasing concentration of alginate and reached a maximum yield of 3.12 ± 0.18 U ml
−1
at 2% (w/v). The immobilized cells produced xylanase consistently up to 10 cycles and reached a maximum level at the forth cycle (3.36 ± 0.2 U ml
−1
). |
| doi_str_mv | 10.1007/s10295-007-0292-7 |
| format | Article |
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Trichoderma reesei
SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous fermentation was also studied. The influence of alginate concentration (1, 2, 3, and 4%) and initial cell loading (100, 200, 300, 400, and 500 beads per flask) on xylanase production was considered. The production of xylanase was found to increase significantly with increasing concentration of alginate and reached a maximum yield of 3.12 ± 0.18 U ml
−1
at 2% (w/v). The immobilized cells produced xylanase consistently up to 10 cycles and reached a maximum level at the forth cycle (3.36 ± 0.2 U ml
−1
).</description><identifier>ISSN: 1367-5435</identifier><identifier>EISSN: 1476-5535</identifier><identifier>DOI: 10.1007/s10295-007-0292-7</identifier><identifier>PMID: 18180968</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Alginates - chemistry ; Biochemistry ; Bioengineering ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Bioreactors ; Biotechnology ; Catalysts ; Cells, Immobilized - enzymology ; Chemical oxygen demand ; Endo-1,4-beta Xylanases - chemistry ; Endo-1,4-beta Xylanases - metabolism ; Enzymes ; Fermentation ; Fundamental and applied biological sciences. Psychology ; Fungal Proteins - metabolism ; General aspects ; Genetic Engineering ; Glucuronic Acid - chemistry ; Hexuronic Acids - chemistry ; Hypocrea jecorina ; Immobilization techniques ; India ; Industrial Microbiology ; Inorganic Chemistry ; Life Sciences ; Methods. Procedures. Technologies ; Microbiology ; Microspheres ; Original Paper ; Spores, Fungal - enzymology ; Studies ; Substrate Specificity ; Trichoderma - enzymology ; Trichoderma - isolation & purification</subject><ispartof>Journal of industrial microbiology & biotechnology, 2008-04, Vol.35 (4), p.245-249</ispartof><rights>Society for Industrial Microbiology 2007</rights><rights>2008 INIST-CNRS</rights><rights>Society for Industrial Microbiology 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-745ff5309676473d4aabf82c837dc98f8a03eabc578a524e68726b97abc9afc73</citedby><cites>FETCH-LOGICAL-c539t-745ff5309676473d4aabf82c837dc98f8a03eabc578a524e68726b97abc9afc73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10295-007-0292-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10295-007-0292-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20223666$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18180968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kar, Sanjay</creatorcontrib><creatorcontrib>Mandal, Asish</creatorcontrib><creatorcontrib>Mohapatra, Pradeep K. Das</creatorcontrib><creatorcontrib>Samanta, Saptadip</creatorcontrib><creatorcontrib>Pati, Bikash R.</creatorcontrib><creatorcontrib>Mondal, Keshab C.</creatorcontrib><title>Production of xylanase by immobilized Trichoderma reesei SAF3 in Ca-alginate beads</title><title>Journal of industrial microbiology & biotechnology</title><addtitle>J Ind Microbiol Biotechnol</addtitle><addtitle>J Ind Microbiol Biotechnol</addtitle><description>In the present study, the optimum conditions for the production of xylanase by immobilized spores of
Trichoderma reesei
SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous fermentation was also studied. The influence of alginate concentration (1, 2, 3, and 4%) and initial cell loading (100, 200, 300, 400, and 500 beads per flask) on xylanase production was considered. The production of xylanase was found to increase significantly with increasing concentration of alginate and reached a maximum yield of 3.12 ± 0.18 U ml
−1
at 2% (w/v). The immobilized cells produced xylanase consistently up to 10 cycles and reached a maximum level at the forth cycle (3.36 ± 0.2 U ml
−1
).</description><subject>Alginates - chemistry</subject><subject>Biochemistry</subject><subject>Bioengineering</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Bioreactors</subject><subject>Biotechnology</subject><subject>Catalysts</subject><subject>Cells, Immobilized - enzymology</subject><subject>Chemical oxygen demand</subject><subject>Endo-1,4-beta Xylanases - chemistry</subject><subject>Endo-1,4-beta Xylanases - metabolism</subject><subject>Enzymes</subject><subject>Fermentation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal Proteins - metabolism</subject><subject>General aspects</subject><subject>Genetic Engineering</subject><subject>Glucuronic Acid - chemistry</subject><subject>Hexuronic Acids - chemistry</subject><subject>Hypocrea jecorina</subject><subject>Immobilization techniques</subject><subject>India</subject><subject>Industrial Microbiology</subject><subject>Inorganic Chemistry</subject><subject>Life Sciences</subject><subject>Methods. Procedures. 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Das ; Samanta, Saptadip ; Pati, Bikash R. ; Mondal, Keshab C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c539t-745ff5309676473d4aabf82c837dc98f8a03eabc578a524e68726b97abc9afc73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Alginates - chemistry</topic><topic>Biochemistry</topic><topic>Bioengineering</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Bioreactors</topic><topic>Biotechnology</topic><topic>Catalysts</topic><topic>Cells, Immobilized - enzymology</topic><topic>Chemical oxygen demand</topic><topic>Endo-1,4-beta Xylanases - chemistry</topic><topic>Endo-1,4-beta Xylanases - metabolism</topic><topic>Enzymes</topic><topic>Fermentation</topic><topic>Fundamental and applied biological sciences. 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Das</au><au>Samanta, Saptadip</au><au>Pati, Bikash R.</au><au>Mondal, Keshab C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of xylanase by immobilized Trichoderma reesei SAF3 in Ca-alginate beads</atitle><jtitle>Journal of industrial microbiology & biotechnology</jtitle><stitle>J Ind Microbiol Biotechnol</stitle><addtitle>J Ind Microbiol Biotechnol</addtitle><date>2008-04-01</date><risdate>2008</risdate><volume>35</volume><issue>4</issue><spage>245</spage><epage>249</epage><pages>245-249</pages><issn>1367-5435</issn><eissn>1476-5535</eissn><abstract>In the present study, the optimum conditions for the production of xylanase by immobilized spores of
Trichoderma reesei
SAF3 in calcium alginate beads were determined. The operational stability of the beads during xylanase production under semi-continuous fermentation was also studied. The influence of alginate concentration (1, 2, 3, and 4%) and initial cell loading (100, 200, 300, 400, and 500 beads per flask) on xylanase production was considered. The production of xylanase was found to increase significantly with increasing concentration of alginate and reached a maximum yield of 3.12 ± 0.18 U ml
−1
at 2% (w/v). The immobilized cells produced xylanase consistently up to 10 cycles and reached a maximum level at the forth cycle (3.36 ± 0.2 U ml
−1
).</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>18180968</pmid><doi>10.1007/s10295-007-0292-7</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of industrial microbiology & biotechnology, 2008-04, Vol.35 (4), p.245-249 |
| issn | 1367-5435 1476-5535 |
| language | eng |
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| subjects | Alginates - chemistry Biochemistry Bioengineering Bioinformatics Biological and medical sciences Biomedical and Life Sciences Bioreactors Biotechnology Catalysts Cells, Immobilized - enzymology Chemical oxygen demand Endo-1,4-beta Xylanases - chemistry Endo-1,4-beta Xylanases - metabolism Enzymes Fermentation Fundamental and applied biological sciences. Psychology Fungal Proteins - metabolism General aspects Genetic Engineering Glucuronic Acid - chemistry Hexuronic Acids - chemistry Hypocrea jecorina Immobilization techniques India Industrial Microbiology Inorganic Chemistry Life Sciences Methods. Procedures. Technologies Microbiology Microspheres Original Paper Spores, Fungal - enzymology Studies Substrate Specificity Trichoderma - enzymology Trichoderma - isolation & purification |
| title | Production of xylanase by immobilized Trichoderma reesei SAF3 in Ca-alginate beads |
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