Glycyrrhizin inhibits interleukin-8 production and nuclear factor-kappaB activity in lung epithelial cells, but not through glucocorticoid receptors

This study was designed to examine the glucocorticoid-like inhibitory effect of glycyrrhizin (GL) on interleukin (IL)-8 production in A549 lung epithelial cells. GL, as well as dexamethasone (DEX) inhibited both tumor necrosis factor (TNF)-alpha- and IL-1beta-induced IL-8 production, mRNA expression...

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Veröffentlicht in:Journal of pharmacological sciences 2008-03, Vol.106 (3), p.460-468
Hauptverfasser: Takei, Hironori, Baba, Yuichiro, Hisatsune, Akinori, Katsuki, Hiroshi, Miyata, Takeshi, Yokomizo, Kazumi, Isohama, Yoichiro
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Sprache:eng
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Zusammenfassung:This study was designed to examine the glucocorticoid-like inhibitory effect of glycyrrhizin (GL) on interleukin (IL)-8 production in A549 lung epithelial cells. GL, as well as dexamethasone (DEX) inhibited both tumor necrosis factor (TNF)-alpha- and IL-1beta-induced IL-8 production, mRNA expression, and promoter activity in A549 cells. Both GL and DEX inhibited transactivation of nuclear factor (NF)-kappaB, without inhibiting translocation of the NF-kappaB p65 subunit to the nucleus. However, the effect of GL was insensitive to RU486, a GR antagonist, and to GR knockdown by siRNA. Furthermore, only GL inhibited DNA binding of p65 to the IL-8 promoter region. These findings indicated that GL had a glucocorticoid-like inhibitory effect on IL-8 production via a mechanism that differs from that of glucocorticoids.
ISSN:1347-8613