The VP1 protein of avian encephalomyelitis virus is a major host-protective immunogen that serves as diagnostic potential
Avian encephalomyelitis virus (AEV) is an important pathogen of poultry and is classified as a member of Picornaviridae. To investigate the protective immunity induced by AEV structural proteins, recombinant VP1, VP0, and VP3 proteins were expressed in a baculovirus system. The result of in vivo pro...
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| Veröffentlicht in: | Journal of virological methods 2008-04, Vol.149 (1), p.56-62 |
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| creator | Wei, Li Chee, Li Lian Wei, Ting Kwang, Jimmy Zhou, Jiao Wang, Jing Shi, Lei Liu, Jue |
| description | Avian encephalomyelitis virus (AEV) is an important pathogen of poultry and is classified as a member of
Picornaviridae. To investigate the protective immunity induced by AEV structural proteins, recombinant VP1, VP0, and VP3 proteins were expressed in a baculovirus system. The result of in vivo protection assays shows that the VP1 protein is a major host-protective immunogen against AEV challenge and demonstrates further that the antibody raised against VP1 protein could neutralize more effectively AEV infection than antibody against VP3 or VP0 protein in a virus neutralization test. These purified recombinant proteins were subsequently evaluated as enzyme-linked immunosorbent assay (ELISA) antigens for detection of AEV infection. A total number of 50 positive sera and 30 negative sera were tested for ELISA validation. Results obtained by testing 193 sera from chickens suspected of being infected AEV further showed that the diagnostic sensitivities of the VP1, VP3, and VP0 protein-based ELISAs were 98.1, 80.6, and 51.9%, and their specificities were 100, 87.9, and 81.8%, respectively. Both sensitivity and specificity of the VP1 protein-based ELISA were comparable with a commercially available test, indicating that the VP1 protein has a highly promising and reliable diagnostic potential, and thus is a suitable antigen for ELISA detection of AEV antibodies in chickens. |
| doi_str_mv | 10.1016/j.jviromet.2008.01.006 |
| format | Article |
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Picornaviridae. To investigate the protective immunity induced by AEV structural proteins, recombinant VP1, VP0, and VP3 proteins were expressed in a baculovirus system. The result of in vivo protection assays shows that the VP1 protein is a major host-protective immunogen against AEV challenge and demonstrates further that the antibody raised against VP1 protein could neutralize more effectively AEV infection than antibody against VP3 or VP0 protein in a virus neutralization test. These purified recombinant proteins were subsequently evaluated as enzyme-linked immunosorbent assay (ELISA) antigens for detection of AEV infection. A total number of 50 positive sera and 30 negative sera were tested for ELISA validation. Results obtained by testing 193 sera from chickens suspected of being infected AEV further showed that the diagnostic sensitivities of the VP1, VP3, and VP0 protein-based ELISAs were 98.1, 80.6, and 51.9%, and their specificities were 100, 87.9, and 81.8%, respectively. Both sensitivity and specificity of the VP1 protein-based ELISA were comparable with a commercially available test, indicating that the VP1 protein has a highly promising and reliable diagnostic potential, and thus is a suitable antigen for ELISA detection of AEV antibodies in chickens.</description><subject>Animals</subject><subject>Antibodies, Viral - blood</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigenicity</subject><subject>Avian encephalomyelitis virus (AEV)</subject><subject>Baculovirus</subject><subject>Biological and medical sciences</subject><subject>Chickens - immunology</subject><subject>Chickens - virology</subject><subject>Diagnostic potential</subject><subject>Encephalomyelitis Virus, Avian - immunology</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunogenicity</subject><subject>Microbiology</subject><subject>Picornaviridae</subject><subject>Picornaviridae Infections - diagnosis</subject><subject>Picornaviridae Infections - immunology</subject><subject>Picornaviridae Infections - prevention & control</subject><subject>Picornaviridae Infections - veterinary</subject><subject>Poultry Diseases - diagnosis</subject><subject>Poultry Diseases - immunology</subject><subject>Poultry Diseases - prevention & control</subject><subject>Recombinant Proteins - immunology</subject><subject>Sensitivity and Specificity</subject><subject>Techniques used in virology</subject><subject>Viral Structural Proteins - immunology</subject><subject>Virology</subject><subject>VP1 protein</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EotvCK1S-wC3BjhM7ewNVQJEqwWHF1Zra466jJF5sJ9K-PV52gWNPM4fvnxnNR8gtZzVnXH4Y6mH1MUyY64axvma8Zky-IBveq23Ftn37kmwKKEsv2ityndLAGOuUEK_JFe8Fl1zwDTnu9kh__uD0EENGP9PgKKweZoqzwcMexjAdcfTZJ1oWLomWBugEQ4h0H1Ku_gRN9itSP03LHJ5wpnkPmSaMKxY6UevhaS6wN_RQ6Dl7GN-QVw7GhG8v9Ybsvnze3d1XD9-_frv79FCZtlG5alpQ0BpnLLTcdK53nWq3kjXOATgLVkjhOucMKKOE6a3tlEQjGe-sfezEDXl_Hlvu_LVgynryyeA4woxhSVqxlivRyWfBhinVioYVUJ5BE0NKEZ0-RD9BPGrO9EmOHvRfOfokRzOui5wSvL1sWB4ntP9jFxsFeHcBIBkYXYTZ-PSPa1gjRNecuI9nDsvfVo9RJ-NPvqyPRYW2wT93y2-CVrSd</recordid><startdate>20080401</startdate><enddate>20080401</enddate><creator>Wei, Li</creator><creator>Chee, Li Lian</creator><creator>Wei, Ting</creator><creator>Kwang, Jimmy</creator><creator>Zhou, Jiao</creator><creator>Wang, Jing</creator><creator>Shi, Lei</creator><creator>Liu, Jue</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080401</creationdate><title>The VP1 protein of avian encephalomyelitis virus is a major host-protective immunogen that serves as diagnostic potential</title><author>Wei, Li ; Chee, Li Lian ; Wei, Ting ; Kwang, Jimmy ; Zhou, Jiao ; Wang, Jing ; Shi, Lei ; Liu, Jue</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-24a7a4cfcda41c5f8f5749602ffaafdad363f5ffca7c73c8dd576ec6015ddb53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antibodies, Viral - blood</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigenicity</topic><topic>Avian encephalomyelitis virus (AEV)</topic><topic>Baculovirus</topic><topic>Biological and medical sciences</topic><topic>Chickens - immunology</topic><topic>Chickens - virology</topic><topic>Diagnostic potential</topic><topic>Encephalomyelitis Virus, Avian - immunology</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunogenicity</topic><topic>Microbiology</topic><topic>Picornaviridae</topic><topic>Picornaviridae Infections - diagnosis</topic><topic>Picornaviridae Infections - immunology</topic><topic>Picornaviridae Infections - prevention & control</topic><topic>Picornaviridae Infections - veterinary</topic><topic>Poultry Diseases - diagnosis</topic><topic>Poultry Diseases - immunology</topic><topic>Poultry Diseases - prevention & control</topic><topic>Recombinant Proteins - immunology</topic><topic>Sensitivity and Specificity</topic><topic>Techniques used in virology</topic><topic>Viral Structural Proteins - immunology</topic><topic>Virology</topic><topic>VP1 protein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wei, Li</creatorcontrib><creatorcontrib>Chee, Li Lian</creatorcontrib><creatorcontrib>Wei, Ting</creatorcontrib><creatorcontrib>Kwang, Jimmy</creatorcontrib><creatorcontrib>Zhou, Jiao</creatorcontrib><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Shi, Lei</creatorcontrib><creatorcontrib>Liu, Jue</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wei, Li</au><au>Chee, Li Lian</au><au>Wei, Ting</au><au>Kwang, Jimmy</au><au>Zhou, Jiao</au><au>Wang, Jing</au><au>Shi, Lei</au><au>Liu, Jue</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The VP1 protein of avian encephalomyelitis virus is a major host-protective immunogen that serves as diagnostic potential</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2008-04-01</date><risdate>2008</risdate><volume>149</volume><issue>1</issue><spage>56</spage><epage>62</epage><pages>56-62</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>Avian encephalomyelitis virus (AEV) is an important pathogen of poultry and is classified as a member of
Picornaviridae. To investigate the protective immunity induced by AEV structural proteins, recombinant VP1, VP0, and VP3 proteins were expressed in a baculovirus system. The result of in vivo protection assays shows that the VP1 protein is a major host-protective immunogen against AEV challenge and demonstrates further that the antibody raised against VP1 protein could neutralize more effectively AEV infection than antibody against VP3 or VP0 protein in a virus neutralization test. These purified recombinant proteins were subsequently evaluated as enzyme-linked immunosorbent assay (ELISA) antigens for detection of AEV infection. A total number of 50 positive sera and 30 negative sera were tested for ELISA validation. Results obtained by testing 193 sera from chickens suspected of being infected AEV further showed that the diagnostic sensitivities of the VP1, VP3, and VP0 protein-based ELISAs were 98.1, 80.6, and 51.9%, and their specificities were 100, 87.9, and 81.8%, respectively. Both sensitivity and specificity of the VP1 protein-based ELISA were comparable with a commercially available test, indicating that the VP1 protein has a highly promising and reliable diagnostic potential, and thus is a suitable antigen for ELISA detection of AEV antibodies in chickens.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>18316131</pmid><doi>10.1016/j.jviromet.2008.01.006</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of virological methods, 2008-04, Vol.149 (1), p.56-62 |
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| subjects | Animals Antibodies, Viral - blood Antibodies, Viral - immunology Antigenicity Avian encephalomyelitis virus (AEV) Baculovirus Biological and medical sciences Chickens - immunology Chickens - virology Diagnostic potential Encephalomyelitis Virus, Avian - immunology Enzyme-Linked Immunosorbent Assay - methods Fundamental and applied biological sciences. Psychology Immunogenicity Microbiology Picornaviridae Picornaviridae Infections - diagnosis Picornaviridae Infections - immunology Picornaviridae Infections - prevention & control Picornaviridae Infections - veterinary Poultry Diseases - diagnosis Poultry Diseases - immunology Poultry Diseases - prevention & control Recombinant Proteins - immunology Sensitivity and Specificity Techniques used in virology Viral Structural Proteins - immunology Virology VP1 protein |
| title | The VP1 protein of avian encephalomyelitis virus is a major host-protective immunogen that serves as diagnostic potential |
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