Orientation of Cutinase Adsorbed onto PMMA Nanoparticles Probed by Tryptophan Fluorescence

The fluorescence of the single tryptophan (Trp69) of cutinase from Fusarium solani pisi, free in aqueous solution and adsorbed onto the surface of poly(methyl methacrylate) (PMMA) latex particles, was studied at pHs of 4.5 and 8.0. The monodisperse PMMA particles (d = 106.0 ± 0.1 nm) were coated wit...

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Veröffentlicht in:The journal of physical chemistry. B 2008-03, Vol.112 (12), p.3581-3585
Hauptverfasser: Santos, Andrea M, Fedorov, Aleksander, Martinho, José M. G, Baptista, Ricardo P, Taipa, Maria Ângela, Cabral, Joaquim M. S
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Sprache:eng
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