Lactobacillus GG has in vitro effects on enhanced interleukin-10 and interferon-γ release of mononuclear cells but no in vivo effects in supplemented mothers and their neonates
Summary Background The value of probiotics for primary prevention is controversial. Moreover, only little is known about the underlying immunological mechanisms of action. Therefore, we assessed the proliferative response and cytokine release in cultures of isolated mononuclear cells from pregnant w...
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| Veröffentlicht in: | Clinical and experimental allergy 2008-04, Vol.38 (4), p.602-610 |
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| creator | Kopp, M. V. Goldstein, M. Dietschek, A. Sofke, J. Heinzmann, A. Urbanek, R. |
| description | Summary
Background
The value of probiotics for primary prevention is controversial. Moreover, only little is known about the underlying immunological mechanisms of action. Therefore, we assessed the proliferative response and cytokine release in cultures of isolated mononuclear cells from pregnant women and their neonates supplemented with Lactobacillus GG (LGG) or placebo.
Methods
In a double‐blind, placebo‐controlled prospective trial, pregnant women with at least one first‐degree relative or a partner with an atopic disease were randomly assigned to receive either the probiotic LGG (ATCC 53103; 5 × 109 colony‐forming units LGG twice daily) or placebo 4–6 weeks before expected delivery, followed by a post‐natal period of 6 months. Cord blood mononuclear cells (CBMC) and peripheral blood mononuclear cells (PBMC) of the corresponding mother were isolated from cord blood and peripheral blood (n=68). The proliferative response of CBMC and PBMC was expressed as the stimulation index (SI), which was calculated according to the ratio between the mean counts per minute (c.p.m.) values measured in the wells with stimulated cells and the mean c.p.m. values measured in the wells with unstimulated cells. Additionally, the cytokines IFN‐γ, IL‐10 and IL‐13 in the cell culture supernatants were measured using the ELISA technique.
Results
No difference was observed between the LGG‐supplemented group and the placebo group in terms of the proliferative capacity of maternal or neonatal cord blood cells in response to IL‐2, β‐lactoglobulin or LGG. In vitro stimulation with LGG resulted in significantly enhanced release of IL‐10 and IFN‐γ, compared with cytokine release in unstimulated controls. However, this phenomenon was observed in supernatants of maternal and neonatal MC in both groups, independent of prior supplementation with LGG.
Conclusion
LGG has in vitro effects on enhanced IL‐10 and IFN‐γ release of mononuclear cells. However, supplementation with LGG during pregnancy did not alter the proliferative capacity or cytokine pattern in their recipients. |
| doi_str_mv | 10.1111/j.1365-2222.2007.02911.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70412544</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70412544</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4351-73fe1297268130119072501fcc1f698dd427f4a00bc70fd524596f4ea6ddbdc13</originalsourceid><addsrcrecordid>eNqNkc-O0zAQxiMEYsvCKyBf4JbicZw4OXBYVbvlT1UkBOJoOc5YTTexi50s3cdCvAfPhLOpulfm4hn7982M9SUJAbqEGO_2S8iKPGUxloxSsaSsAlgenySL88PTZEGrnKeirPhF8iKEPaU0y6vyeXIBJRQCGCyS3xulB1cr3XbdGMh6TXYqkNaSu3bwjqAxqIdAnCVod8pqbOLjgL7D8ba1KVCi7OnKoHc2_fuHeOxQBSTOkN5ZZ0cda080dl0g9TgQ6-YJd48DYh3Gw6HDHmOvJgqHHfrw0D1mrScWnVUDhpfJM6O6gK9O52Xy_eb62-pDuvmy_ri62qSaZzmkIjMIrBKsKCGjABUVLKdgtAZTVGXTcCYMV5TWWlDT5IznVWE4qqJp6kZDdpm8nfsevPs5Yhhk34bpDypuMgYpKAeWcx7Bcga1dyF4NPLg2175ewlUTnbJvZxckZMrcrJLPtglj1H6-jRjrHtsHoUnfyLw5gSooFVnfLSgDWeOUQbAaR659zP3q-3w_r8XkKvrqymL-nTWt2HA41mv_K0sRCZy-WO7ltuvBft8Iz7JbfYPiAjCxg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70412544</pqid></control><display><type>article</type><title>Lactobacillus GG has in vitro effects on enhanced interleukin-10 and interferon-γ release of mononuclear cells but no in vivo effects in supplemented mothers and their neonates</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Kopp, M. V. ; Goldstein, M. ; Dietschek, A. ; Sofke, J. ; Heinzmann, A. ; Urbanek, R.</creator><creatorcontrib>Kopp, M. V. ; Goldstein, M. ; Dietschek, A. ; Sofke, J. ; Heinzmann, A. ; Urbanek, R.</creatorcontrib><description>Summary
Background
The value of probiotics for primary prevention is controversial. Moreover, only little is known about the underlying immunological mechanisms of action. Therefore, we assessed the proliferative response and cytokine release in cultures of isolated mononuclear cells from pregnant women and their neonates supplemented with Lactobacillus GG (LGG) or placebo.
Methods
In a double‐blind, placebo‐controlled prospective trial, pregnant women with at least one first‐degree relative or a partner with an atopic disease were randomly assigned to receive either the probiotic LGG (ATCC 53103; 5 × 109 colony‐forming units LGG twice daily) or placebo 4–6 weeks before expected delivery, followed by a post‐natal period of 6 months. Cord blood mononuclear cells (CBMC) and peripheral blood mononuclear cells (PBMC) of the corresponding mother were isolated from cord blood and peripheral blood (n=68). The proliferative response of CBMC and PBMC was expressed as the stimulation index (SI), which was calculated according to the ratio between the mean counts per minute (c.p.m.) values measured in the wells with stimulated cells and the mean c.p.m. values measured in the wells with unstimulated cells. Additionally, the cytokines IFN‐γ, IL‐10 and IL‐13 in the cell culture supernatants were measured using the ELISA technique.
Results
No difference was observed between the LGG‐supplemented group and the placebo group in terms of the proliferative capacity of maternal or neonatal cord blood cells in response to IL‐2, β‐lactoglobulin or LGG. In vitro stimulation with LGG resulted in significantly enhanced release of IL‐10 and IFN‐γ, compared with cytokine release in unstimulated controls. However, this phenomenon was observed in supernatants of maternal and neonatal MC in both groups, independent of prior supplementation with LGG.
Conclusion
LGG has in vitro effects on enhanced IL‐10 and IFN‐γ release of mononuclear cells. However, supplementation with LGG during pregnancy did not alter the proliferative capacity or cytokine pattern in their recipients.</description><identifier>ISSN: 0954-7894</identifier><identifier>EISSN: 1365-2222</identifier><identifier>DOI: 10.1111/j.1365-2222.2007.02911.x</identifier><identifier>PMID: 18167121</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; allergy ; Biological and medical sciences ; Cell Proliferation ; Cells, Cultured ; Cohort Studies ; cord blood ; cytokine release ; Double-Blind Method ; Enzyme-Linked Immunosorbent Assay ; Female ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Infant, Newborn ; Interferon-gamma - analysis ; Interferon-gamma - metabolism ; Interferon-gamma - secretion ; Interleukin-10 - analysis ; Interleukin-10 - metabolism ; Interleukin-10 - secretion ; Interleukin-13 - analysis ; Interleukin-13 - metabolism ; Interleukin-13 - secretion ; Lactobacillus - immunology ; Lactobacillus GG ; Leukocytes, Mononuclear - immunology ; Leukocytes, Mononuclear - secretion ; Male ; Medical sciences ; Mothers ; Placebos ; Predictive Value of Tests ; Pregnancy ; prevention ; probiotics ; Probiotics - administration & dosage ; Prospective Studies ; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><ispartof>Clinical and experimental allergy, 2008-04, Vol.38 (4), p.602-610</ispartof><rights>2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4351-73fe1297268130119072501fcc1f698dd427f4a00bc70fd524596f4ea6ddbdc13</citedby><cites>FETCH-LOGICAL-c4351-73fe1297268130119072501fcc1f698dd427f4a00bc70fd524596f4ea6ddbdc13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2222.2007.02911.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2222.2007.02911.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20211405$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18167121$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kopp, M. V.</creatorcontrib><creatorcontrib>Goldstein, M.</creatorcontrib><creatorcontrib>Dietschek, A.</creatorcontrib><creatorcontrib>Sofke, J.</creatorcontrib><creatorcontrib>Heinzmann, A.</creatorcontrib><creatorcontrib>Urbanek, R.</creatorcontrib><title>Lactobacillus GG has in vitro effects on enhanced interleukin-10 and interferon-γ release of mononuclear cells but no in vivo effects in supplemented mothers and their neonates</title><title>Clinical and experimental allergy</title><addtitle>Clin Exp Allergy</addtitle><description>Summary
Background
The value of probiotics for primary prevention is controversial. Moreover, only little is known about the underlying immunological mechanisms of action. Therefore, we assessed the proliferative response and cytokine release in cultures of isolated mononuclear cells from pregnant women and their neonates supplemented with Lactobacillus GG (LGG) or placebo.
Methods
In a double‐blind, placebo‐controlled prospective trial, pregnant women with at least one first‐degree relative or a partner with an atopic disease were randomly assigned to receive either the probiotic LGG (ATCC 53103; 5 × 109 colony‐forming units LGG twice daily) or placebo 4–6 weeks before expected delivery, followed by a post‐natal period of 6 months. Cord blood mononuclear cells (CBMC) and peripheral blood mononuclear cells (PBMC) of the corresponding mother were isolated from cord blood and peripheral blood (n=68). The proliferative response of CBMC and PBMC was expressed as the stimulation index (SI), which was calculated according to the ratio between the mean counts per minute (c.p.m.) values measured in the wells with stimulated cells and the mean c.p.m. values measured in the wells with unstimulated cells. Additionally, the cytokines IFN‐γ, IL‐10 and IL‐13 in the cell culture supernatants were measured using the ELISA technique.
Results
No difference was observed between the LGG‐supplemented group and the placebo group in terms of the proliferative capacity of maternal or neonatal cord blood cells in response to IL‐2, β‐lactoglobulin or LGG. In vitro stimulation with LGG resulted in significantly enhanced release of IL‐10 and IFN‐γ, compared with cytokine release in unstimulated controls. However, this phenomenon was observed in supernatants of maternal and neonatal MC in both groups, independent of prior supplementation with LGG.
Conclusion
LGG has in vitro effects on enhanced IL‐10 and IFN‐γ release of mononuclear cells. However, supplementation with LGG during pregnancy did not alter the proliferative capacity or cytokine pattern in their recipients.</description><subject>Adult</subject><subject>allergy</subject><subject>Biological and medical sciences</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Cohort Studies</subject><subject>cord blood</subject><subject>cytokine release</subject><subject>Double-Blind Method</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Interferon-gamma - analysis</subject><subject>Interferon-gamma - metabolism</subject><subject>Interferon-gamma - secretion</subject><subject>Interleukin-10 - analysis</subject><subject>Interleukin-10 - metabolism</subject><subject>Interleukin-10 - secretion</subject><subject>Interleukin-13 - analysis</subject><subject>Interleukin-13 - metabolism</subject><subject>Interleukin-13 - secretion</subject><subject>Lactobacillus - immunology</subject><subject>Lactobacillus GG</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Leukocytes, Mononuclear - secretion</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mothers</subject><subject>Placebos</subject><subject>Predictive Value of Tests</subject><subject>Pregnancy</subject><subject>prevention</subject><subject>probiotics</subject><subject>Probiotics - administration & dosage</subject><subject>Prospective Studies</subject><subject>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</subject><issn>0954-7894</issn><issn>1365-2222</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-O0zAQxiMEYsvCKyBf4JbicZw4OXBYVbvlT1UkBOJoOc5YTTexi50s3cdCvAfPhLOpulfm4hn7982M9SUJAbqEGO_2S8iKPGUxloxSsaSsAlgenySL88PTZEGrnKeirPhF8iKEPaU0y6vyeXIBJRQCGCyS3xulB1cr3XbdGMh6TXYqkNaSu3bwjqAxqIdAnCVod8pqbOLjgL7D8ba1KVCi7OnKoHc2_fuHeOxQBSTOkN5ZZ0cda080dl0g9TgQ6-YJd48DYh3Gw6HDHmOvJgqHHfrw0D1mrScWnVUDhpfJM6O6gK9O52Xy_eb62-pDuvmy_ri62qSaZzmkIjMIrBKsKCGjABUVLKdgtAZTVGXTcCYMV5TWWlDT5IznVWE4qqJp6kZDdpm8nfsevPs5Yhhk34bpDypuMgYpKAeWcx7Bcga1dyF4NPLg2175ewlUTnbJvZxckZMrcrJLPtglj1H6-jRjrHtsHoUnfyLw5gSooFVnfLSgDWeOUQbAaR659zP3q-3w_r8XkKvrqymL-nTWt2HA41mv_K0sRCZy-WO7ltuvBft8Iz7JbfYPiAjCxg</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Kopp, M. V.</creator><creator>Goldstein, M.</creator><creator>Dietschek, A.</creator><creator>Sofke, J.</creator><creator>Heinzmann, A.</creator><creator>Urbanek, R.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>Lactobacillus GG has in vitro effects on enhanced interleukin-10 and interferon-γ release of mononuclear cells but no in vivo effects in supplemented mothers and their neonates</title><author>Kopp, M. V. ; Goldstein, M. ; Dietschek, A. ; Sofke, J. ; Heinzmann, A. ; Urbanek, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4351-73fe1297268130119072501fcc1f698dd427f4a00bc70fd524596f4ea6ddbdc13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>allergy</topic><topic>Biological and medical sciences</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Cohort Studies</topic><topic>cord blood</topic><topic>cytokine release</topic><topic>Double-Blind Method</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Interferon-gamma - analysis</topic><topic>Interferon-gamma - metabolism</topic><topic>Interferon-gamma - secretion</topic><topic>Interleukin-10 - analysis</topic><topic>Interleukin-10 - metabolism</topic><topic>Interleukin-10 - secretion</topic><topic>Interleukin-13 - analysis</topic><topic>Interleukin-13 - metabolism</topic><topic>Interleukin-13 - secretion</topic><topic>Lactobacillus - immunology</topic><topic>Lactobacillus GG</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Leukocytes, Mononuclear - secretion</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mothers</topic><topic>Placebos</topic><topic>Predictive Value of Tests</topic><topic>Pregnancy</topic><topic>prevention</topic><topic>probiotics</topic><topic>Probiotics - administration & dosage</topic><topic>Prospective Studies</topic><topic>Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kopp, M. V.</creatorcontrib><creatorcontrib>Goldstein, M.</creatorcontrib><creatorcontrib>Dietschek, A.</creatorcontrib><creatorcontrib>Sofke, J.</creatorcontrib><creatorcontrib>Heinzmann, A.</creatorcontrib><creatorcontrib>Urbanek, R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental allergy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kopp, M. V.</au><au>Goldstein, M.</au><au>Dietschek, A.</au><au>Sofke, J.</au><au>Heinzmann, A.</au><au>Urbanek, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactobacillus GG has in vitro effects on enhanced interleukin-10 and interferon-γ release of mononuclear cells but no in vivo effects in supplemented mothers and their neonates</atitle><jtitle>Clinical and experimental allergy</jtitle><addtitle>Clin Exp Allergy</addtitle><date>2008-04</date><risdate>2008</risdate><volume>38</volume><issue>4</issue><spage>602</spage><epage>610</epage><pages>602-610</pages><issn>0954-7894</issn><eissn>1365-2222</eissn><abstract>Summary
Background
The value of probiotics for primary prevention is controversial. Moreover, only little is known about the underlying immunological mechanisms of action. Therefore, we assessed the proliferative response and cytokine release in cultures of isolated mononuclear cells from pregnant women and their neonates supplemented with Lactobacillus GG (LGG) or placebo.
Methods
In a double‐blind, placebo‐controlled prospective trial, pregnant women with at least one first‐degree relative or a partner with an atopic disease were randomly assigned to receive either the probiotic LGG (ATCC 53103; 5 × 109 colony‐forming units LGG twice daily) or placebo 4–6 weeks before expected delivery, followed by a post‐natal period of 6 months. Cord blood mononuclear cells (CBMC) and peripheral blood mononuclear cells (PBMC) of the corresponding mother were isolated from cord blood and peripheral blood (n=68). The proliferative response of CBMC and PBMC was expressed as the stimulation index (SI), which was calculated according to the ratio between the mean counts per minute (c.p.m.) values measured in the wells with stimulated cells and the mean c.p.m. values measured in the wells with unstimulated cells. Additionally, the cytokines IFN‐γ, IL‐10 and IL‐13 in the cell culture supernatants were measured using the ELISA technique.
Results
No difference was observed between the LGG‐supplemented group and the placebo group in terms of the proliferative capacity of maternal or neonatal cord blood cells in response to IL‐2, β‐lactoglobulin or LGG. In vitro stimulation with LGG resulted in significantly enhanced release of IL‐10 and IFN‐γ, compared with cytokine release in unstimulated controls. However, this phenomenon was observed in supernatants of maternal and neonatal MC in both groups, independent of prior supplementation with LGG.
Conclusion
LGG has in vitro effects on enhanced IL‐10 and IFN‐γ release of mononuclear cells. However, supplementation with LGG during pregnancy did not alter the proliferative capacity or cytokine pattern in their recipients.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18167121</pmid><doi>10.1111/j.1365-2222.2007.02911.x</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Clinical and experimental allergy, 2008-04, Vol.38 (4), p.602-610 |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Adult allergy Biological and medical sciences Cell Proliferation Cells, Cultured Cohort Studies cord blood cytokine release Double-Blind Method Enzyme-Linked Immunosorbent Assay Female Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Infant, Newborn Interferon-gamma - analysis Interferon-gamma - metabolism Interferon-gamma - secretion Interleukin-10 - analysis Interleukin-10 - metabolism Interleukin-10 - secretion Interleukin-13 - analysis Interleukin-13 - metabolism Interleukin-13 - secretion Lactobacillus - immunology Lactobacillus GG Leukocytes, Mononuclear - immunology Leukocytes, Mononuclear - secretion Male Medical sciences Mothers Placebos Predictive Value of Tests Pregnancy prevention probiotics Probiotics - administration & dosage Prospective Studies Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis |
| title | Lactobacillus GG has in vitro effects on enhanced interleukin-10 and interferon-γ release of mononuclear cells but no in vivo effects in supplemented mothers and their neonates |
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