Ultrastructural co-localization of cochlin and type II collagen in the rat semicircular canal
Cochlin and type II collagen are major constituents of the inner ear extracellular matrix. To investigate the morphological relation of cochlin and type II collagen in the rat semicircular canal, immuno-electronmicroscopic analysis was performed using the post-embedding immunogold method. Immunolabe...
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| Veröffentlicht in: | Neuroscience letters 2008-03, Vol.434 (1), p.104-107 |
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| container_title | Neuroscience letters |
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| creator | Mizuta, Kunihiro Ikezono, Tetsuo Iwasaki, Satoshi Arai, Maki Hashimoto, Yasuyuki Pawankar, Ruby Watanabe, Takahiro Shindo, Susumu Mineta, Hiroyuki |
| description | Cochlin and type II collagen are major constituents of the inner ear extracellular matrix. To investigate the morphological relation of cochlin and type II collagen in the rat semicircular canal, immuno-electronmicroscopic analysis was performed using the post-embedding immunogold method. Immunolabeling for cochlin was detected in the fibrillar substance underlying the supporting epithelium of the sensory cells and beneath the epithelial cells facing the endolymph in the semicircular canals. Immunolabeling for type II collagen was observed in the same fibrillar substance in the subepithelial area. The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles. |
| doi_str_mv | 10.1016/j.neulet.2008.01.036 |
| format | Article |
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To investigate the morphological relation of cochlin and type II collagen in the rat semicircular canal, immuno-electronmicroscopic analysis was performed using the post-embedding immunogold method. Immunolabeling for cochlin was detected in the fibrillar substance underlying the supporting epithelium of the sensory cells and beneath the epithelial cells facing the endolymph in the semicircular canals. Immunolabeling for type II collagen was observed in the same fibrillar substance in the subepithelial area. The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2008.01.036</identifier><identifier>PMID: 18304733</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Basement Membrane - metabolism ; Basement Membrane - ultrastructure ; Biological and medical sciences ; COCH ; Collagen Type II - metabolism ; DFNA9 ; Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation ; Epithelial Cells - metabolism ; Epithelial Cells - ultrastructure ; Extracellular Matrix - metabolism ; Extracellular Matrix - ultrastructure ; Extracellular Matrix Proteins - metabolism ; Fundamental and applied biological sciences. Psychology ; Hair Cells, Ampulla - metabolism ; Hair Cells, Ampulla - ultrastructure ; Hereditary hearing impairment ; Immunogold method ; Microscopy, Immunoelectron ; Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration ; Nerve Tissue Proteins - metabolism ; Proteins - metabolism ; Rats ; Rats, Wistar ; Semicircular Canals - metabolism ; Semicircular Canals - ultrastructure ; Vertebrates: nervous system and sense organs ; Vestibular organ</subject><ispartof>Neuroscience letters, 2008-03, Vol.434 (1), p.104-107</ispartof><rights>2008 Elsevier Ireland Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-fe027f9175eda3f8c8ac31c3028e1f87f6f206c5876d195cc249cecb329066183</citedby><cites>FETCH-LOGICAL-c456t-fe027f9175eda3f8c8ac31c3028e1f87f6f206c5876d195cc249cecb329066183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.neulet.2008.01.036$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20187560$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18304733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mizuta, Kunihiro</creatorcontrib><creatorcontrib>Ikezono, Tetsuo</creatorcontrib><creatorcontrib>Iwasaki, Satoshi</creatorcontrib><creatorcontrib>Arai, Maki</creatorcontrib><creatorcontrib>Hashimoto, Yasuyuki</creatorcontrib><creatorcontrib>Pawankar, Ruby</creatorcontrib><creatorcontrib>Watanabe, Takahiro</creatorcontrib><creatorcontrib>Shindo, Susumu</creatorcontrib><creatorcontrib>Mineta, Hiroyuki</creatorcontrib><title>Ultrastructural co-localization of cochlin and type II collagen in the rat semicircular canal</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>Cochlin and type II collagen are major constituents of the inner ear extracellular matrix. To investigate the morphological relation of cochlin and type II collagen in the rat semicircular canal, immuno-electronmicroscopic analysis was performed using the post-embedding immunogold method. Immunolabeling for cochlin was detected in the fibrillar substance underlying the supporting epithelium of the sensory cells and beneath the epithelial cells facing the endolymph in the semicircular canals. Immunolabeling for type II collagen was observed in the same fibrillar substance in the subepithelial area. The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles.</description><subject>Animals</subject><subject>Basement Membrane - metabolism</subject><subject>Basement Membrane - ultrastructure</subject><subject>Biological and medical sciences</subject><subject>COCH</subject><subject>Collagen Type II - metabolism</subject><subject>DFNA9</subject><subject>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - ultrastructure</subject><subject>Extracellular Matrix - metabolism</subject><subject>Extracellular Matrix - ultrastructure</subject><subject>Extracellular Matrix Proteins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hair Cells, Ampulla - metabolism</subject><subject>Hair Cells, Ampulla - ultrastructure</subject><subject>Hereditary hearing impairment</subject><subject>Immunogold method</subject><subject>Microscopy, Immunoelectron</subject><subject>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Semicircular Canals - metabolism</subject><subject>Semicircular Canals - ultrastructure</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Vestibular organ</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1r3DAQhkVpaLZp_0EpurQ3OyPJtuxLoYR-LAR6aY5BKONRo0UrbyU5kP76KOzS3noaeOeZYfSIsXcCWgFiuNy1kdZApZUAYwuiBTW8YBsxatnoScuXbAMKukZNHZyz1znvAKAXffeKnYuxdrRSG3Z7E0qyuaQVy5ps4Lg0YUEb_B9b_BL54mqE98FHbuPMy-OB-HZbsxDsL4q85uWeeLKFZ9p79AnXYBNHG214w86cDZnenuoFu_n65efV9-b6x7ft1efrBrt-KI0jkNpNQvc0W-VGHC0qgQrkSMKN2g1OwoD9qIdZTD2i7CYkvFNygmGoj7lgH497D2n5vVIuZu8zUj0x0rJmo6GDqVdTBbsjiGnJOZEzh-T3Nj0aAeZZq9mZo1bzrNWAMFVrHXt_2r_e7Wn-N3TyWIEPJ8DmKs8lG9Hnv5yE-i39AJX7dOSo2njwlExGTxFp9omwmHnx_7_kCVZjmGM</recordid><startdate>20080321</startdate><enddate>20080321</enddate><creator>Mizuta, Kunihiro</creator><creator>Ikezono, Tetsuo</creator><creator>Iwasaki, Satoshi</creator><creator>Arai, Maki</creator><creator>Hashimoto, Yasuyuki</creator><creator>Pawankar, Ruby</creator><creator>Watanabe, Takahiro</creator><creator>Shindo, Susumu</creator><creator>Mineta, Hiroyuki</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080321</creationdate><title>Ultrastructural co-localization of cochlin and type II collagen in the rat semicircular canal</title><author>Mizuta, Kunihiro ; Ikezono, Tetsuo ; Iwasaki, Satoshi ; Arai, Maki ; Hashimoto, Yasuyuki ; Pawankar, Ruby ; Watanabe, Takahiro ; Shindo, Susumu ; Mineta, Hiroyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-fe027f9175eda3f8c8ac31c3028e1f87f6f206c5876d195cc249cecb329066183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Basement Membrane - metabolism</topic><topic>Basement Membrane - ultrastructure</topic><topic>Biological and medical sciences</topic><topic>COCH</topic><topic>Collagen Type II - metabolism</topic><topic>DFNA9</topic><topic>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - ultrastructure</topic><topic>Extracellular Matrix - metabolism</topic><topic>Extracellular Matrix - ultrastructure</topic><topic>Extracellular Matrix Proteins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hair Cells, Ampulla - metabolism</topic><topic>Hair Cells, Ampulla - ultrastructure</topic><topic>Hereditary hearing impairment</topic><topic>Immunogold method</topic><topic>Microscopy, Immunoelectron</topic><topic>Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Proteins - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Semicircular Canals - metabolism</topic><topic>Semicircular Canals - ultrastructure</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Vestibular organ</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mizuta, Kunihiro</creatorcontrib><creatorcontrib>Ikezono, Tetsuo</creatorcontrib><creatorcontrib>Iwasaki, Satoshi</creatorcontrib><creatorcontrib>Arai, Maki</creatorcontrib><creatorcontrib>Hashimoto, Yasuyuki</creatorcontrib><creatorcontrib>Pawankar, Ruby</creatorcontrib><creatorcontrib>Watanabe, Takahiro</creatorcontrib><creatorcontrib>Shindo, Susumu</creatorcontrib><creatorcontrib>Mineta, Hiroyuki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mizuta, Kunihiro</au><au>Ikezono, Tetsuo</au><au>Iwasaki, Satoshi</au><au>Arai, Maki</au><au>Hashimoto, Yasuyuki</au><au>Pawankar, Ruby</au><au>Watanabe, Takahiro</au><au>Shindo, Susumu</au><au>Mineta, Hiroyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrastructural co-localization of cochlin and type II collagen in the rat semicircular canal</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2008-03-21</date><risdate>2008</risdate><volume>434</volume><issue>1</issue><spage>104</spage><epage>107</epage><pages>104-107</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>Cochlin and type II collagen are major constituents of the inner ear extracellular matrix. To investigate the morphological relation of cochlin and type II collagen in the rat semicircular canal, immuno-electronmicroscopic analysis was performed using the post-embedding immunogold method. Immunolabeling for cochlin was detected in the fibrillar substance underlying the supporting epithelium of the sensory cells and beneath the epithelial cells facing the endolymph in the semicircular canals. Immunolabeling for type II collagen was observed in the same fibrillar substance in the subepithelial area. The co-localization of cochlin and type II collagen in the fibrillar substance in the subepithelial area indicate that cochlin may play a role in the structural homeostasis of the vestibule acting in concert with the fibrillar type II collagen bundles.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>18304733</pmid><doi>10.1016/j.neulet.2008.01.036</doi><tpages>4</tpages></addata></record> |
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| subjects | Animals Basement Membrane - metabolism Basement Membrane - ultrastructure Biological and medical sciences COCH Collagen Type II - metabolism DFNA9 Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation Epithelial Cells - metabolism Epithelial Cells - ultrastructure Extracellular Matrix - metabolism Extracellular Matrix - ultrastructure Extracellular Matrix Proteins - metabolism Fundamental and applied biological sciences. Psychology Hair Cells, Ampulla - metabolism Hair Cells, Ampulla - ultrastructure Hereditary hearing impairment Immunogold method Microscopy, Immunoelectron Motor control and motor pathways. Reflexes. Control centers of vegetative functions. Vestibular system and equilibration Nerve Tissue Proteins - metabolism Proteins - metabolism Rats Rats, Wistar Semicircular Canals - metabolism Semicircular Canals - ultrastructure Vertebrates: nervous system and sense organs Vestibular organ |
| title | Ultrastructural co-localization of cochlin and type II collagen in the rat semicircular canal |
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