Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots
Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-gluta...
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| Veröffentlicht in: | Planta 2007-05, Vol.225 (6), p.1597-1602 |
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| creator | Innocenti, Gilles Pucciariello, Chiara Le Gleuher, Marie Hopkins, Julie de Stefano, Matteo Delledonne, Massimo Puppo, Alain Baudouin, Emmanuel Frendo, Pierre |
| description | Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthetase (γ-ECS), glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) after treatment with sodium nitroprusside (SNP) and nitrosoglutathione (GSNO), two NO-donors, showed that γ-ecs and gshs genes are up regulated by NO treatment whereas hgshs expression is not. Differential accumulation of GSH was correlated to gene expression in SNP-treated roots. Our results provide the first evidence that GSH synthesis pathway is regulated by NO in plants and that there is a differential regulation between gshs and hgshs in M. truncatula. |
| doi_str_mv | 10.1007/s00425-006-0461-3 |
| format | Article |
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Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthetase (γ-ECS), glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) after treatment with sodium nitroprusside (SNP) and nitrosoglutathione (GSNO), two NO-donors, showed that γ-ecs and gshs genes are up regulated by NO treatment whereas hgshs expression is not. Differential accumulation of GSH was correlated to gene expression in SNP-treated roots. Our results provide the first evidence that GSH synthesis pathway is regulated by NO in plants and that there is a differential regulation between gshs and hgshs in M. truncatula.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-006-0461-3</identifier><identifier>PMID: 17195940</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Agronomy. Soil science and plant productions ; Alfalfa ; Biological and medical sciences ; Economic plant physiology ; Fundamental and applied biological sciences. Psychology ; Gene expression regulation ; Gene Expression Regulation, Plant ; Genes ; Glutathione - biosynthesis ; Heavy metals ; Legumes ; Medicago truncatula - metabolism ; Nitric oxide ; Nitric Oxide - metabolism ; Oxides ; Plant Proteins - metabolism ; Plant roots ; Plant Roots - metabolism ; Plants ; RAPID COMMUNICATION ; Reactive oxygen species ; RNA ; Roots ; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) ; Thiols</subject><ispartof>Planta, 2007-05, Vol.225 (6), p.1597-1602</ispartof><rights>Springer-Verlag Berlin Heidelberg 2007</rights><rights>2007 INIST-CNRS</rights><rights>Springer-Verlag 2006.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-d55db28534bc7b149ca12737da699f19d53cc884c4175eee9de15651974c60ba3</citedby><cites>FETCH-LOGICAL-c378t-d55db28534bc7b149ca12737da699f19d53cc884c4175eee9de15651974c60ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23389740$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23389740$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18717730$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17195940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Innocenti, Gilles</creatorcontrib><creatorcontrib>Pucciariello, Chiara</creatorcontrib><creatorcontrib>Le Gleuher, Marie</creatorcontrib><creatorcontrib>Hopkins, Julie</creatorcontrib><creatorcontrib>de Stefano, Matteo</creatorcontrib><creatorcontrib>Delledonne, Massimo</creatorcontrib><creatorcontrib>Puppo, Alain</creatorcontrib><creatorcontrib>Baudouin, Emmanuel</creatorcontrib><creatorcontrib>Frendo, Pierre</creatorcontrib><title>Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots</title><title>Planta</title><addtitle>Planta</addtitle><description>Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthetase (γ-ECS), glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) after treatment with sodium nitroprusside (SNP) and nitrosoglutathione (GSNO), two NO-donors, showed that γ-ecs and gshs genes are up regulated by NO treatment whereas hgshs expression is not. Differential accumulation of GSH was correlated to gene expression in SNP-treated roots. Our results provide the first evidence that GSH synthesis pathway is regulated by NO in plants and that there is a differential regulation between gshs and hgshs in M. truncatula.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Alfalfa</subject><subject>Biological and medical sciences</subject><subject>Economic plant physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression regulation</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Glutathione - biosynthesis</subject><subject>Heavy metals</subject><subject>Legumes</subject><subject>Medicago truncatula - metabolism</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - metabolism</subject><subject>Oxides</subject><subject>Plant Proteins - metabolism</subject><subject>Plant roots</subject><subject>Plant Roots - metabolism</subject><subject>Plants</subject><subject>RAPID COMMUNICATION</subject><subject>Reactive oxygen species</subject><subject>RNA</subject><subject>Roots</subject><subject>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</subject><subject>Thiols</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpd0FFrHCEQB3ApKc017QfIQ4IU2rdtZ1Zd9TGENi2k5KV9Fle9xGNvTdSF3rePxx0NBASF-c0w_gk5R_iKAPJbAeC96ACGDviAHXtDVshZ3_XA1QlZAbQ3aCZOyftSNgCtKOU7cooStdAcVuTuZlqqrQ8xzYGW3VwfQomFtpPD_TLZGjwdd3SONUdH07_oA40z_R18dPY-0ZqX2dnaJM0p1fKBvF3bqYSPx_uM_P3x_c_1z-727ubX9dVt55hUtfNC-LFXgvHRyRG5dhZ7yaS3g9Zr1F4w55TijqMUIQTtA4pBoJbcDTBadka-HOY-5vS0hFLNNhYXpsnOIS3FSOAgtYYGP72Cm7Tkue1mFCqlRd9jQ3hALqdSclibxxy3Nu8MgtlHbQ5Rmxa12UdtWOu5PA5exm3wLx3HbBv4fAS2ODuts51dLC9OSZSS7d3FwW1KTfl_vWdMte8CewaO7Y-G</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Innocenti, Gilles</creator><creator>Pucciariello, Chiara</creator><creator>Le Gleuher, Marie</creator><creator>Hopkins, Julie</creator><creator>de Stefano, Matteo</creator><creator>Delledonne, Massimo</creator><creator>Puppo, Alain</creator><creator>Baudouin, Emmanuel</creator><creator>Frendo, Pierre</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070501</creationdate><title>Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots</title><author>Innocenti, Gilles ; Pucciariello, Chiara ; Le Gleuher, Marie ; Hopkins, Julie ; de Stefano, Matteo ; Delledonne, Massimo ; Puppo, Alain ; Baudouin, Emmanuel ; Frendo, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-d55db28534bc7b149ca12737da699f19d53cc884c4175eee9de15651974c60ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Alfalfa</topic><topic>Biological and medical sciences</topic><topic>Economic plant physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression regulation</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Glutathione - biosynthesis</topic><topic>Heavy metals</topic><topic>Legumes</topic><topic>Medicago truncatula - metabolism</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - metabolism</topic><topic>Oxides</topic><topic>Plant Proteins - metabolism</topic><topic>Plant roots</topic><topic>Plant Roots - metabolism</topic><topic>Plants</topic><topic>RAPID COMMUNICATION</topic><topic>Reactive oxygen species</topic><topic>RNA</topic><topic>Roots</topic><topic>Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)</topic><topic>Thiols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Innocenti, Gilles</creatorcontrib><creatorcontrib>Pucciariello, Chiara</creatorcontrib><creatorcontrib>Le Gleuher, Marie</creatorcontrib><creatorcontrib>Hopkins, Julie</creatorcontrib><creatorcontrib>de Stefano, Matteo</creatorcontrib><creatorcontrib>Delledonne, Massimo</creatorcontrib><creatorcontrib>Puppo, Alain</creatorcontrib><creatorcontrib>Baudouin, Emmanuel</creatorcontrib><creatorcontrib>Frendo, Pierre</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Innocenti, Gilles</au><au>Pucciariello, Chiara</au><au>Le Gleuher, Marie</au><au>Hopkins, Julie</au><au>de Stefano, Matteo</au><au>Delledonne, Massimo</au><au>Puppo, Alain</au><au>Baudouin, Emmanuel</au><au>Frendo, Pierre</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>225</volume><issue>6</issue><spage>1597</spage><epage>1602</epage><pages>1597-1602</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthetase (γ-ECS), glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) after treatment with sodium nitroprusside (SNP) and nitrosoglutathione (GSNO), two NO-donors, showed that γ-ecs and gshs genes are up regulated by NO treatment whereas hgshs expression is not. Differential accumulation of GSH was correlated to gene expression in SNP-treated roots. Our results provide the first evidence that GSH synthesis pathway is regulated by NO in plants and that there is a differential regulation between gshs and hgshs in M. truncatula.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>17195940</pmid><doi>10.1007/s00425-006-0461-3</doi><tpages>6</tpages></addata></record> |
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| subjects | Agronomy. Soil science and plant productions Alfalfa Biological and medical sciences Economic plant physiology Fundamental and applied biological sciences. Psychology Gene expression regulation Gene Expression Regulation, Plant Genes Glutathione - biosynthesis Heavy metals Legumes Medicago truncatula - metabolism Nitric oxide Nitric Oxide - metabolism Oxides Plant Proteins - metabolism Plant roots Plant Roots - metabolism Plants RAPID COMMUNICATION Reactive oxygen species RNA Roots Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...) Thiols |
| title | Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots |
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