upstream region in the first intron of petunia actin-depolymerizing factor 1 affects tissue-specific expression in transgenic Arabidopsis (Arabidopsis thaliana)
The first intron of the petunia actin-depolymerizing factor 1 (PhADF1) gene was previously shown to induce strong and constitutive expression of that gene in vegetative tissues of transgenic Arabidopsis. To examine intron-mediated enhancement of PhADF1 gene expression in detail, the effects of splic...
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| Veröffentlicht in: | The Plant journal : for cell and molecular biology 2007-04, Vol.50 (2), p.230-239 |
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| creator | Jeong, Young-Min Mun, Jeong-Hwan Kim, Hoyeun Lee, So-Young Kim, Sang-Gu |
| description | The first intron of the petunia actin-depolymerizing factor 1 (PhADF1) gene was previously shown to induce strong and constitutive expression of that gene in vegetative tissues of transgenic Arabidopsis. To examine intron-mediated enhancement of PhADF1 gene expression in detail, the effects of splicing, deletion and promoter alteration on gene expression were analyzed in this study. Deletion of the 5' upstream region of the intron significantly reduced the level of enhancement, under the control of both the PhADF1 and the PhADF2 promoters. The ratio of pre-mRNA and mRNA does not correlate with the level of enhancement. To determine whether there is a promoter-intron interaction, the role of the intron was examined under the control of a heterogeneous promoter. The intron of PhADF1 induced GUS expression in vegetative tissues under the control of the reproductive tissue-specific Arabidopsis profilin 5 (PRF5) promoter. In transient assays, the presence of the intron increased GUS expression under control of the 35S minimal promoter. Our results suggest that the first intron of the PhADF1 gene alters tissue-specific expression by a post-transcriptional mechanism. In addition, we have also shown that intron-mediated enhancement is a conserved mechanism, which regulates the expression of the petunia and Arabidopsis ADF genes that are expressed in vegetative tissues. |
| doi_str_mv | 10.1111/j.1365-313x.2007.03053.x |
| format | Article |
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To examine intron-mediated enhancement of PhADF1 gene expression in detail, the effects of splicing, deletion and promoter alteration on gene expression were analyzed in this study. Deletion of the 5' upstream region of the intron significantly reduced the level of enhancement, under the control of both the PhADF1 and the PhADF2 promoters. The ratio of pre-mRNA and mRNA does not correlate with the level of enhancement. To determine whether there is a promoter-intron interaction, the role of the intron was examined under the control of a heterogeneous promoter. The intron of PhADF1 induced GUS expression in vegetative tissues under the control of the reproductive tissue-specific Arabidopsis profilin 5 (PRF5) promoter. In transient assays, the presence of the intron increased GUS expression under control of the 35S minimal promoter. Our results suggest that the first intron of the PhADF1 gene alters tissue-specific expression by a post-transcriptional mechanism. In addition, we have also shown that intron-mediated enhancement is a conserved mechanism, which regulates the expression of the petunia and Arabidopsis ADF genes that are expressed in vegetative tissues.</description><identifier>ISSN: 0960-7412</identifier><identifier>EISSN: 1365-313X</identifier><identifier>DOI: 10.1111/j.1365-313x.2007.03053.x</identifier><identifier>PMID: 17376165</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Actin Depolymerizing Factors - genetics ; Actin Depolymerizing Factors - metabolism ; actin-depolymerizing factor ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis thaliana ; Biological and medical sciences ; Blotting, Northern ; Botany ; Evolution, Molecular ; Fluorometry ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Plant ; Glucuronidase - genetics ; Glucuronidase - metabolism ; GUS ; intron ; intron-mediated enhancement ; Introns - genetics ; Molecular and cellular biology ; Molecular genetics ; Mutation ; Petunia ; Petunia - genetics ; Plants, Genetically Modified ; Profilins - genetics ; Promoter Regions, Genetic - genetics ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - metabolism ; Ribonucleic acid ; RNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; tissue-specificity ; Tissues ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing ; Transgenic plants</subject><ispartof>The Plant journal : for cell and molecular biology, 2007-04, Vol.50 (2), p.230-239</ispartof><rights>2007 INIST-CNRS</rights><rights>2007 The Authors Journal compilation 2007 Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5953-fb6e06d36fc960effccdc50a824ed094bced62a6f79fca0b4249de8ad00137b03</citedby><cites>FETCH-LOGICAL-c5953-fb6e06d36fc960effccdc50a824ed094bced62a6f79fca0b4249de8ad00137b03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-313X.2007.03053.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-313X.2007.03053.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18671485$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17376165$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jeong, Young-Min</creatorcontrib><creatorcontrib>Mun, Jeong-Hwan</creatorcontrib><creatorcontrib>Kim, Hoyeun</creatorcontrib><creatorcontrib>Lee, So-Young</creatorcontrib><creatorcontrib>Kim, Sang-Gu</creatorcontrib><title>upstream region in the first intron of petunia actin-depolymerizing factor 1 affects tissue-specific expression in transgenic Arabidopsis (Arabidopsis thaliana)</title><title>The Plant journal : for cell and molecular biology</title><addtitle>Plant J</addtitle><description>The first intron of the petunia actin-depolymerizing factor 1 (PhADF1) gene was previously shown to induce strong and constitutive expression of that gene in vegetative tissues of transgenic Arabidopsis. To examine intron-mediated enhancement of PhADF1 gene expression in detail, the effects of splicing, deletion and promoter alteration on gene expression were analyzed in this study. Deletion of the 5' upstream region of the intron significantly reduced the level of enhancement, under the control of both the PhADF1 and the PhADF2 promoters. The ratio of pre-mRNA and mRNA does not correlate with the level of enhancement. To determine whether there is a promoter-intron interaction, the role of the intron was examined under the control of a heterogeneous promoter. The intron of PhADF1 induced GUS expression in vegetative tissues under the control of the reproductive tissue-specific Arabidopsis profilin 5 (PRF5) promoter. In transient assays, the presence of the intron increased GUS expression under control of the 35S minimal promoter. Our results suggest that the first intron of the PhADF1 gene alters tissue-specific expression by a post-transcriptional mechanism. In addition, we have also shown that intron-mediated enhancement is a conserved mechanism, which regulates the expression of the petunia and Arabidopsis ADF genes that are expressed in vegetative tissues.</description><subject>Actin Depolymerizing Factors - genetics</subject><subject>Actin Depolymerizing Factors - metabolism</subject><subject>actin-depolymerizing factor</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Botany</subject><subject>Evolution, Molecular</subject><subject>Fluorometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Glucuronidase - genetics</subject><subject>Glucuronidase - metabolism</subject><subject>GUS</subject><subject>intron</subject><subject>intron-mediated enhancement</subject><subject>Introns - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Mutation</subject><subject>Petunia</subject><subject>Petunia - genetics</subject><subject>Plants, Genetically Modified</subject><subject>Profilins - genetics</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>tissue-specificity</subject><subject>Tissues</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Transgenic plants</subject><issn>0960-7412</issn><issn>1365-313X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAQxyMEotvCK4CFBIJDgh07dnLgUFV8qhJItBI3y3HGW6_yVU8idvs0PCpOd6siLuDLeMa_Gc_MP0kIoxmL5-0mY1wWKWd8m-WUqoxyWvBs-yBZ3T38eJisaCVpqgTLj5JjxA2lTHEpHidH0SrJZLFKfs0jTgFMRwKs_dAT35PpCojzAafoTCHGBkdGmObeG2Ls5Pu0gXFodx0Ef-P7NXExOgTCiHEO7IRk8ogzpDiC9c5bAtsxAOJd_WB6XEMfH06DqX0zjOiRvP7Tma5M601v3jxJHjnTIjw92JPk8sP7i7NP6fnXj5_PTs9TW1QFT10tgcqGS2fj0OCctY0tqClzAQ2tRG2hkbmRTlXOGlqLXFQNlKaJS-GqpvwkebWvO4bhegacdOfRQtuaHoYZtaKCKpXLf4I5FVwUZRHBF3-Bm2EOfRxC54wXrCpFGaFyD9kwIAZwegy-M2GnGdWL1nqjF0n1IqletNa3WuttTH12qD_XHTT3iQdxI_DyABi0pnVx7dbjPVdKxcRto-_23E_fwu6_G9AX374st5j_fJ_vzKDNOsQ_Lr_nca8RllJF4jcxHdJ3</recordid><startdate>200704</startdate><enddate>200704</enddate><creator>Jeong, Young-Min</creator><creator>Mun, Jeong-Hwan</creator><creator>Kim, Hoyeun</creator><creator>Lee, So-Young</creator><creator>Kim, Sang-Gu</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200704</creationdate><title>upstream region in the first intron of petunia actin-depolymerizing factor 1 affects tissue-specific expression in transgenic Arabidopsis (Arabidopsis thaliana)</title><author>Jeong, Young-Min ; Mun, Jeong-Hwan ; Kim, Hoyeun ; Lee, So-Young ; Kim, Sang-Gu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5953-fb6e06d36fc960effccdc50a824ed094bced62a6f79fca0b4249de8ad00137b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Actin Depolymerizing Factors - genetics</topic><topic>Actin Depolymerizing Factors - metabolism</topic><topic>actin-depolymerizing factor</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Botany</topic><topic>Evolution, Molecular</topic><topic>Fluorometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Glucuronidase - genetics</topic><topic>Glucuronidase - metabolism</topic><topic>GUS</topic><topic>intron</topic><topic>intron-mediated enhancement</topic><topic>Introns - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Mutation</topic><topic>Petunia</topic><topic>Petunia - genetics</topic><topic>Plants, Genetically Modified</topic><topic>Profilins - genetics</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>tissue-specificity</topic><topic>Tissues</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jeong, Young-Min</creatorcontrib><creatorcontrib>Mun, Jeong-Hwan</creatorcontrib><creatorcontrib>Kim, Hoyeun</creatorcontrib><creatorcontrib>Lee, So-Young</creatorcontrib><creatorcontrib>Kim, Sang-Gu</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Plant journal : for cell and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jeong, Young-Min</au><au>Mun, Jeong-Hwan</au><au>Kim, Hoyeun</au><au>Lee, So-Young</au><au>Kim, Sang-Gu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>upstream region in the first intron of petunia actin-depolymerizing factor 1 affects tissue-specific expression in transgenic Arabidopsis (Arabidopsis thaliana)</atitle><jtitle>The Plant journal : for cell and molecular biology</jtitle><addtitle>Plant J</addtitle><date>2007-04</date><risdate>2007</risdate><volume>50</volume><issue>2</issue><spage>230</spage><epage>239</epage><pages>230-239</pages><issn>0960-7412</issn><eissn>1365-313X</eissn><abstract>The first intron of the petunia actin-depolymerizing factor 1 (PhADF1) gene was previously shown to induce strong and constitutive expression of that gene in vegetative tissues of transgenic Arabidopsis. To examine intron-mediated enhancement of PhADF1 gene expression in detail, the effects of splicing, deletion and promoter alteration on gene expression were analyzed in this study. Deletion of the 5' upstream region of the intron significantly reduced the level of enhancement, under the control of both the PhADF1 and the PhADF2 promoters. The ratio of pre-mRNA and mRNA does not correlate with the level of enhancement. To determine whether there is a promoter-intron interaction, the role of the intron was examined under the control of a heterogeneous promoter. The intron of PhADF1 induced GUS expression in vegetative tissues under the control of the reproductive tissue-specific Arabidopsis profilin 5 (PRF5) promoter. In transient assays, the presence of the intron increased GUS expression under control of the 35S minimal promoter. Our results suggest that the first intron of the PhADF1 gene alters tissue-specific expression by a post-transcriptional mechanism. In addition, we have also shown that intron-mediated enhancement is a conserved mechanism, which regulates the expression of the petunia and Arabidopsis ADF genes that are expressed in vegetative tissues.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>17376165</pmid><doi>10.1111/j.1365-313x.2007.03053.x</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actin Depolymerizing Factors - genetics Actin Depolymerizing Factors - metabolism actin-depolymerizing factor Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis thaliana Biological and medical sciences Blotting, Northern Botany Evolution, Molecular Fluorometry Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Plant Glucuronidase - genetics Glucuronidase - metabolism GUS intron intron-mediated enhancement Introns - genetics Molecular and cellular biology Molecular genetics Mutation Petunia Petunia - genetics Plants, Genetically Modified Profilins - genetics Promoter Regions, Genetic - genetics Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - metabolism Ribonucleic acid RNA RNA, Messenger - genetics RNA, Messenger - metabolism tissue-specificity Tissues Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing Transgenic plants |
| title | upstream region in the first intron of petunia actin-depolymerizing factor 1 affects tissue-specific expression in transgenic Arabidopsis (Arabidopsis thaliana) |
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