Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles
Recent design-based stereological methods that can be applied to thick sections cut in an arbitrary direction are presented and their implementation for measuring mesophyll anatomical characteristics is introduced. These methods use software-randomized virtual 3D probes, such as disector and fakir t...
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| Veröffentlicht in: | Journal of experimental botany 2007-01, Vol.58 (6), p.1451-1461 |
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| container_issue | 6 |
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| container_title | Journal of experimental botany |
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| creator | ALBRECHTOVA, Jana JANACEK, Jiri LHOTAKOVA, Zuzana RADOCHOVA, Barbora KUBINOVA, Lucie |
| description | Recent design-based stereological methods that can be applied to thick sections cut in an arbitrary direction are presented and their implementation for measuring mesophyll anatomical characteristics is introduced. These methods use software-randomized virtual 3D probes, such as disector and fakir test probes, in stacks of optical sections acquired using confocal microscopy. They enable unbiased estimations of the mean mesophyll cell volume, mesophyll cell number in a needle, and for the first time an internal surface area of needles or other narrow leaves directly from the fresh tissue cross-sections cut using a hand microtome. Therefore, reliable results can be obtained much faster than when using a standard microtechnical preparation. The proposed methods were tested on Norway spruce needles affected for 1 year by acid rain treatment. The effect of acid rain resulted in changes of mesophyll parameters: the ratio of intercellular spaces per mesophyll cell volume increased, while needle internal surface area, total number of mesophyll cells, and number of mesophyll cells per unit volume of a needle decreased in the treated needles. |
| doi_str_mv | 10.1093/jxb/erm007 |
| format | Article |
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These methods use software-randomized virtual 3D probes, such as disector and fakir test probes, in stacks of optical sections acquired using confocal microscopy. They enable unbiased estimations of the mean mesophyll cell volume, mesophyll cell number in a needle, and for the first time an internal surface area of needles or other narrow leaves directly from the fresh tissue cross-sections cut using a hand microtome. Therefore, reliable results can be obtained much faster than when using a standard microtechnical preparation. The proposed methods were tested on Norway spruce needles affected for 1 year by acid rain treatment. The effect of acid rain resulted in changes of mesophyll parameters: the ratio of intercellular spaces per mesophyll cell volume increased, while needle internal surface area, total number of mesophyll cells, and number of mesophyll cells per unit volume of a needle decreased in the treated needles.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/erm007</identifier><identifier>PMID: 17322549</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Acid rain ; Anatomy ; Biological and medical sciences ; Carbon Dioxide - metabolism ; Confocal microscopy ; Density ; disector method ; Estimation methods ; Extracellular space ; fakir method ; Forest management. Stand types and stand dynamics. Silvicultural treatments. Tending of stands. Natural regeneration ; Forestry ; Fundamental and applied biological sciences. Psychology ; internal surface area ; Light ; Mesophyll ; Mesophyll cells ; Microscopy ; Microscopy, Confocal ; Norway spruce ; Photosynthesis ; Picea - cytology ; Plant anatomy ; Plant Leaves - cytology ; Plant Leaves - physiology ; Rain ; Research Papers ; Stand types and stand dynamics. Silvicultural treatments. Tending of stands. Natural regeneration ; stereology ; Surface areas</subject><ispartof>Journal of experimental botany, 2007-01, Vol.58 (6), p.1451-1461</ispartof><rights>Society for Experimental Biology 2007</rights><rights>2007 The Author(s). 2007</rights><rights>2007 INIST-CNRS</rights><rights>2007 The Author(s).</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c523t-2251a92b92bc92d90049b4c7fcb5397169e9fec739c4d4d3b87f0ecd63d9b6fc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24036645$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24036645$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,1583,27923,27924,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18765504$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17322549$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ALBRECHTOVA, Jana</creatorcontrib><creatorcontrib>JANACEK, Jiri</creatorcontrib><creatorcontrib>LHOTAKOVA, Zuzana</creatorcontrib><creatorcontrib>RADOCHOVA, Barbora</creatorcontrib><creatorcontrib>KUBINOVA, Lucie</creatorcontrib><title>Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles</title><title>Journal of experimental botany</title><addtitle>J Exp Bot</addtitle><description>Recent design-based stereological methods that can be applied to thick sections cut in an arbitrary direction are presented and their implementation for measuring mesophyll anatomical characteristics is introduced. These methods use software-randomized virtual 3D probes, such as disector and fakir test probes, in stacks of optical sections acquired using confocal microscopy. They enable unbiased estimations of the mean mesophyll cell volume, mesophyll cell number in a needle, and for the first time an internal surface area of needles or other narrow leaves directly from the fresh tissue cross-sections cut using a hand microtome. Therefore, reliable results can be obtained much faster than when using a standard microtechnical preparation. The proposed methods were tested on Norway spruce needles affected for 1 year by acid rain treatment. The effect of acid rain resulted in changes of mesophyll parameters: the ratio of intercellular spaces per mesophyll cell volume increased, while needle internal surface area, total number of mesophyll cells, and number of mesophyll cells per unit volume of a needle decreased in the treated needles.</description><subject>Acid rain</subject><subject>Anatomy</subject><subject>Biological and medical sciences</subject><subject>Carbon Dioxide - metabolism</subject><subject>Confocal microscopy</subject><subject>Density</subject><subject>disector method</subject><subject>Estimation methods</subject><subject>Extracellular space</subject><subject>fakir method</subject><subject>Forest management. Stand types and stand dynamics. Silvicultural treatments. Tending of stands. Natural regeneration</subject><subject>Forestry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>internal surface area</subject><subject>Light</subject><subject>Mesophyll</subject><subject>Mesophyll cells</subject><subject>Microscopy</subject><subject>Microscopy, Confocal</subject><subject>Norway spruce</subject><subject>Photosynthesis</subject><subject>Picea - cytology</subject><subject>Plant anatomy</subject><subject>Plant Leaves - cytology</subject><subject>Plant Leaves - physiology</subject><subject>Rain</subject><subject>Research Papers</subject><subject>Stand types and stand dynamics. Silvicultural treatments. Tending of stands. Natural regeneration</subject><subject>stereology</subject><subject>Surface areas</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkt9rFDEQxxdR7Fl98V0Ngj4Ia7P5sbn0TYq2Qq0UrRRfQjY7e5fr7mZNdrX3B_p_OccdPfBBYUgC85nvJJNvlj0t6NuCan60uq2OIHaUqnvZrBAlzZngxf1sRiljOdVSHWSPUlpRSiWV8mF2UCjOmBR6lv2-CD-hJdA03nnoR9LBuAx1Ik2IeLZpir5f4CmFYbluW2J7O4bOO9sSt7TRuhGiT6N3WBJDhwukJRmX3t2QBG70oU9kShuRhCiENizWqFITF_ombHRQLYbkwrA-JnYYWhTflBEM63xNovV9PkawI9TkIsRfdk3SECcHpAeoW0iPsweNbRM82e2H2dWH919PzvLzz6cfT96d504yPub45sJqVmE4zWpNqdCVcKpxleRaFaUG3YBTXDtRi5pXc9VQcHXJa12VjeOH2eut7hDDjwnSaDqfHLSt7SFMySgqKBdz9l-w0KWUczpH8OVf4CpMscdHGMYlLaRQAqE3W2gzpxShMUP0nY1rU1CzsYBBC5itBRB-vlOcqg7qPbr7cwRe7QCbcPxNtL3zac_NFd6Nij0XpuHfDZ9tuVUaQ7wjGY6iLIXEfL7No03g9i5v440pFVfSnF1_N9_0pShPrz-ZS-RfbPnGBmMX6C5z9YXRgmMzVVDO-B8pzu6U</recordid><startdate>20070101</startdate><enddate>20070101</enddate><creator>ALBRECHTOVA, Jana</creator><creator>JANACEK, Jiri</creator><creator>LHOTAKOVA, Zuzana</creator><creator>RADOCHOVA, Barbora</creator><creator>KUBINOVA, Lucie</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7UA</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20070101</creationdate><title>Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles</title><author>ALBRECHTOVA, Jana ; JANACEK, Jiri ; LHOTAKOVA, Zuzana ; RADOCHOVA, Barbora ; KUBINOVA, Lucie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c523t-2251a92b92bc92d90049b4c7fcb5397169e9fec739c4d4d3b87f0ecd63d9b6fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acid rain</topic><topic>Anatomy</topic><topic>Biological and medical sciences</topic><topic>Carbon Dioxide - metabolism</topic><topic>Confocal microscopy</topic><topic>Density</topic><topic>disector method</topic><topic>Estimation methods</topic><topic>Extracellular space</topic><topic>fakir method</topic><topic>Forest management. Stand types and stand dynamics. Silvicultural treatments. Tending of stands. Natural regeneration</topic><topic>Forestry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>internal surface area</topic><topic>Light</topic><topic>Mesophyll</topic><topic>Mesophyll cells</topic><topic>Microscopy</topic><topic>Microscopy, Confocal</topic><topic>Norway spruce</topic><topic>Photosynthesis</topic><topic>Picea - cytology</topic><topic>Plant anatomy</topic><topic>Plant Leaves - cytology</topic><topic>Plant Leaves - physiology</topic><topic>Rain</topic><topic>Research Papers</topic><topic>Stand types and stand dynamics. Silvicultural treatments. Tending of stands. Natural regeneration</topic><topic>stereology</topic><topic>Surface areas</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ALBRECHTOVA, Jana</creatorcontrib><creatorcontrib>JANACEK, Jiri</creatorcontrib><creatorcontrib>LHOTAKOVA, Zuzana</creatorcontrib><creatorcontrib>RADOCHOVA, Barbora</creatorcontrib><creatorcontrib>KUBINOVA, Lucie</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ALBRECHTOVA, Jana</au><au>JANACEK, Jiri</au><au>LHOTAKOVA, Zuzana</au><au>RADOCHOVA, Barbora</au><au>KUBINOVA, Lucie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles</atitle><jtitle>Journal of experimental botany</jtitle><addtitle>J Exp Bot</addtitle><date>2007-01-01</date><risdate>2007</risdate><volume>58</volume><issue>6</issue><spage>1451</spage><epage>1461</epage><pages>1451-1461</pages><issn>0022-0957</issn><eissn>1460-2431</eissn><coden>JEBOA6</coden><abstract>Recent design-based stereological methods that can be applied to thick sections cut in an arbitrary direction are presented and their implementation for measuring mesophyll anatomical characteristics is introduced. These methods use software-randomized virtual 3D probes, such as disector and fakir test probes, in stacks of optical sections acquired using confocal microscopy. They enable unbiased estimations of the mean mesophyll cell volume, mesophyll cell number in a needle, and for the first time an internal surface area of needles or other narrow leaves directly from the fresh tissue cross-sections cut using a hand microtome. Therefore, reliable results can be obtained much faster than when using a standard microtechnical preparation. The proposed methods were tested on Norway spruce needles affected for 1 year by acid rain treatment. The effect of acid rain resulted in changes of mesophyll parameters: the ratio of intercellular spaces per mesophyll cell volume increased, while needle internal surface area, total number of mesophyll cells, and number of mesophyll cells per unit volume of a needle decreased in the treated needles.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>17322549</pmid><doi>10.1093/jxb/erm007</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Acid rain Anatomy Biological and medical sciences Carbon Dioxide - metabolism Confocal microscopy Density disector method Estimation methods Extracellular space fakir method Forest management. Stand types and stand dynamics. Silvicultural treatments. Tending of stands. Natural regeneration Forestry Fundamental and applied biological sciences. Psychology internal surface area Light Mesophyll Mesophyll cells Microscopy Microscopy, Confocal Norway spruce Photosynthesis Picea - cytology Plant anatomy Plant Leaves - cytology Plant Leaves - physiology Rain Research Papers Stand types and stand dynamics. Silvicultural treatments. Tending of stands. Natural regeneration stereology Surface areas |
| title | Novel efficient methods for measuring mesophyll anatomical characteristics from fresh thick sections using stereology and confocal microscopy: application on acid rain-treated Norway spruce needles |
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