Ultrafast Excited-State Dynamics of the Photoswitchable Protein Dronpa
Dronpa is a photoswitchable protein from the family of green fluorescent proteins (GFPs). Photoswitching involves forward and backward proton transfer between a bright deprotonated form (B) and a dark protonated form (A2). We have used femtosecond transient absorption spectroscopy to determine the k...
Gespeichert in:
| Veröffentlicht in: | Journal of the American Chemical Society 2007-04, Vol.129 (16), p.4870-4871 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4871 |
|---|---|
| container_issue | 16 |
| container_start_page | 4870 |
| container_title | Journal of the American Chemical Society |
| container_volume | 129 |
| creator | Fron, Eduard Flors, Cristina Schweitzer, Gerd Habuchi, Satoshi Mizuno, Hideaki Ando, Ryoko De Schryver, Frans C Miyawaki, Atsushi Hofkens, Johan |
| description | Dronpa is a photoswitchable protein from the family of green fluorescent proteins (GFPs). Photoswitching involves forward and backward proton transfer between a bright deprotonated form (B) and a dark protonated form (A2). We have used femtosecond transient absorption spectroscopy to determine the kinetics of the first step of the photoconversion from the dark to the bright form, which we have measured as 4 ps. The 2-fold isotope effect on the kinetics of this process shows that excited-state proton transfer (ESPT) is involved in this step. It was also demonstrated that the acid-induced protonated form A1 and the photoconverted protonated form A2 are two distinct spectroscopic species. |
| doi_str_mv | 10.1021/ja069365v |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70402276</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70402276</sourcerecordid><originalsourceid>FETCH-LOGICAL-a351t-93e895ea6f49c0e16b61b3098628cb4ce8731919db4520cb460d1ec71382a5723</originalsourceid><addsrcrecordid>eNptkD1PwzAQhi0EglIY-AMoC0gMAX8ktjNCPwCpiEptGVgsx3HUlDQutgPtv8eoVVmYTu_dozvdA8AFgrcIYnS3kJBmhKZfB6CDUgzjFGF6CDoQQhwzTskJOHVuEWKCOToGJ4gRnnKadMBwVnsrS-l8NFiryusinnjpddTfNHJZKReZMvJzHY3nxhv3XXk1l3kdsjVeV03Ut6ZZyTNwVMra6fNd7YLZcDDtPcWj18fn3v0oliRFPs6I5lmqJS2TTEGNaE5RTmDGKeYqT5TmjKAMZUWehDdCh8ICacUQ4VimDJMuuN7uXVnz2WrnxbJySte1bLRpnWAwgRgzGsCbLaiscc7qUqxstZR2IxAUv9LEXlpgL3dL23ypiz9yZykA8RaonNfr_VzaD0EZYamYjicieei_9EfvY_EW-KstL5UTC9PaJjj55_APDEeBbw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70402276</pqid></control><display><type>article</type><title>Ultrafast Excited-State Dynamics of the Photoswitchable Protein Dronpa</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Fron, Eduard ; Flors, Cristina ; Schweitzer, Gerd ; Habuchi, Satoshi ; Mizuno, Hideaki ; Ando, Ryoko ; De Schryver, Frans C ; Miyawaki, Atsushi ; Hofkens, Johan</creator><creatorcontrib>Fron, Eduard ; Flors, Cristina ; Schweitzer, Gerd ; Habuchi, Satoshi ; Mizuno, Hideaki ; Ando, Ryoko ; De Schryver, Frans C ; Miyawaki, Atsushi ; Hofkens, Johan</creatorcontrib><description>Dronpa is a photoswitchable protein from the family of green fluorescent proteins (GFPs). Photoswitching involves forward and backward proton transfer between a bright deprotonated form (B) and a dark protonated form (A2). We have used femtosecond transient absorption spectroscopy to determine the kinetics of the first step of the photoconversion from the dark to the bright form, which we have measured as 4 ps. The 2-fold isotope effect on the kinetics of this process shows that excited-state proton transfer (ESPT) is involved in this step. It was also demonstrated that the acid-induced protonated form A1 and the photoconverted protonated form A2 are two distinct spectroscopic species.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja069365v</identifier><identifier>PMID: 17385864</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Anthozoa - metabolism ; Green Fluorescent Proteins - chemistry ; Photochemistry ; Spectrum Analysis</subject><ispartof>Journal of the American Chemical Society, 2007-04, Vol.129 (16), p.4870-4871</ispartof><rights>Copyright © 2007 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a351t-93e895ea6f49c0e16b61b3098628cb4ce8731919db4520cb460d1ec71382a5723</citedby><cites>FETCH-LOGICAL-a351t-93e895ea6f49c0e16b61b3098628cb4ce8731919db4520cb460d1ec71382a5723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ja069365v$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ja069365v$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17385864$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fron, Eduard</creatorcontrib><creatorcontrib>Flors, Cristina</creatorcontrib><creatorcontrib>Schweitzer, Gerd</creatorcontrib><creatorcontrib>Habuchi, Satoshi</creatorcontrib><creatorcontrib>Mizuno, Hideaki</creatorcontrib><creatorcontrib>Ando, Ryoko</creatorcontrib><creatorcontrib>De Schryver, Frans C</creatorcontrib><creatorcontrib>Miyawaki, Atsushi</creatorcontrib><creatorcontrib>Hofkens, Johan</creatorcontrib><title>Ultrafast Excited-State Dynamics of the Photoswitchable Protein Dronpa</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>Dronpa is a photoswitchable protein from the family of green fluorescent proteins (GFPs). Photoswitching involves forward and backward proton transfer between a bright deprotonated form (B) and a dark protonated form (A2). We have used femtosecond transient absorption spectroscopy to determine the kinetics of the first step of the photoconversion from the dark to the bright form, which we have measured as 4 ps. The 2-fold isotope effect on the kinetics of this process shows that excited-state proton transfer (ESPT) is involved in this step. It was also demonstrated that the acid-induced protonated form A1 and the photoconverted protonated form A2 are two distinct spectroscopic species.</description><subject>Animals</subject><subject>Anthozoa - metabolism</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Photochemistry</subject><subject>Spectrum Analysis</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkD1PwzAQhi0EglIY-AMoC0gMAX8ktjNCPwCpiEptGVgsx3HUlDQutgPtv8eoVVmYTu_dozvdA8AFgrcIYnS3kJBmhKZfB6CDUgzjFGF6CDoQQhwzTskJOHVuEWKCOToGJ4gRnnKadMBwVnsrS-l8NFiryusinnjpddTfNHJZKReZMvJzHY3nxhv3XXk1l3kdsjVeV03Ut6ZZyTNwVMra6fNd7YLZcDDtPcWj18fn3v0oliRFPs6I5lmqJS2TTEGNaE5RTmDGKeYqT5TmjKAMZUWehDdCh8ICacUQ4VimDJMuuN7uXVnz2WrnxbJySte1bLRpnWAwgRgzGsCbLaiscc7qUqxstZR2IxAUv9LEXlpgL3dL23ypiz9yZykA8RaonNfr_VzaD0EZYamYjicieei_9EfvY_EW-KstL5UTC9PaJjj55_APDEeBbw</recordid><startdate>20070425</startdate><enddate>20070425</enddate><creator>Fron, Eduard</creator><creator>Flors, Cristina</creator><creator>Schweitzer, Gerd</creator><creator>Habuchi, Satoshi</creator><creator>Mizuno, Hideaki</creator><creator>Ando, Ryoko</creator><creator>De Schryver, Frans C</creator><creator>Miyawaki, Atsushi</creator><creator>Hofkens, Johan</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070425</creationdate><title>Ultrafast Excited-State Dynamics of the Photoswitchable Protein Dronpa</title><author>Fron, Eduard ; Flors, Cristina ; Schweitzer, Gerd ; Habuchi, Satoshi ; Mizuno, Hideaki ; Ando, Ryoko ; De Schryver, Frans C ; Miyawaki, Atsushi ; Hofkens, Johan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a351t-93e895ea6f49c0e16b61b3098628cb4ce8731919db4520cb460d1ec71382a5723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Anthozoa - metabolism</topic><topic>Green Fluorescent Proteins - chemistry</topic><topic>Photochemistry</topic><topic>Spectrum Analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fron, Eduard</creatorcontrib><creatorcontrib>Flors, Cristina</creatorcontrib><creatorcontrib>Schweitzer, Gerd</creatorcontrib><creatorcontrib>Habuchi, Satoshi</creatorcontrib><creatorcontrib>Mizuno, Hideaki</creatorcontrib><creatorcontrib>Ando, Ryoko</creatorcontrib><creatorcontrib>De Schryver, Frans C</creatorcontrib><creatorcontrib>Miyawaki, Atsushi</creatorcontrib><creatorcontrib>Hofkens, Johan</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fron, Eduard</au><au>Flors, Cristina</au><au>Schweitzer, Gerd</au><au>Habuchi, Satoshi</au><au>Mizuno, Hideaki</au><au>Ando, Ryoko</au><au>De Schryver, Frans C</au><au>Miyawaki, Atsushi</au><au>Hofkens, Johan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ultrafast Excited-State Dynamics of the Photoswitchable Protein Dronpa</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2007-04-25</date><risdate>2007</risdate><volume>129</volume><issue>16</issue><spage>4870</spage><epage>4871</epage><pages>4870-4871</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><abstract>Dronpa is a photoswitchable protein from the family of green fluorescent proteins (GFPs). Photoswitching involves forward and backward proton transfer between a bright deprotonated form (B) and a dark protonated form (A2). We have used femtosecond transient absorption spectroscopy to determine the kinetics of the first step of the photoconversion from the dark to the bright form, which we have measured as 4 ps. The 2-fold isotope effect on the kinetics of this process shows that excited-state proton transfer (ESPT) is involved in this step. It was also demonstrated that the acid-induced protonated form A1 and the photoconverted protonated form A2 are two distinct spectroscopic species.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>17385864</pmid><doi>10.1021/ja069365v</doi><tpages>2</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0002-7863 |
| ispartof | Journal of the American Chemical Society, 2007-04, Vol.129 (16), p.4870-4871 |
| issn | 0002-7863 1520-5126 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70402276 |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Anthozoa - metabolism Green Fluorescent Proteins - chemistry Photochemistry Spectrum Analysis |
| title | Ultrafast Excited-State Dynamics of the Photoswitchable Protein Dronpa |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T01%3A08%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Ultrafast%20Excited-State%20Dynamics%20of%20the%20Photoswitchable%20Protein%20Dronpa&rft.jtitle=Journal%20of%20the%20American%20Chemical%20Society&rft.au=Fron,%20Eduard&rft.date=2007-04-25&rft.volume=129&rft.issue=16&rft.spage=4870&rft.epage=4871&rft.pages=4870-4871&rft.issn=0002-7863&rft.eissn=1520-5126&rft_id=info:doi/10.1021/ja069365v&rft_dat=%3Cproquest_cross%3E70402276%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70402276&rft_id=info:pmid/17385864&rfr_iscdi=true |