Antigen profiles of the fish pathogen Moritella viscosa and protection in fish
Aims: Moritella viscosa is a Gram‐negative bacterium that causes winter ulcer disease in salmonid fish cultured in sea water below 8°C. The aim of this study was to study the antigen profiles of these bacteria and to reveal the protection which the antigens induce in fish. Methods and Results: Lip...
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| Veröffentlicht in: | Journal of applied microbiology 2008-04, Vol.104 (4), p.944-951 |
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| creator | Heidarsdóttir, K.J. Gravningen, K. Benediktsdóttir, E. |
| description | Aims: Moritella viscosa is a Gram‐negative bacterium that causes winter ulcer disease in salmonid fish cultured in sea water below 8°C. The aim of this study was to study the antigen profiles of these bacteria and to reveal the protection which the antigens induce in fish.
Methods and Results: Lipooligosaccharides (LOS) and an approximately 17–19 kDa outer membrane antigen were shown to be the major specific antigens of M. viscosa. The size of the wall antigen differed between strain groups and even between strains reacting positively in the same sera. Four different serotypes of M. viscosa were determined by producing polyclonal sera. Western blot analysis revealed that sera from vaccinated fish groups that had good or fair protection reacted against the LOS and the 17/19 kDa antigen, while no antibody response was observed with sera from groups that showed no efficacy.
Conclusions: The study provides evidence that LOS and an approximately 17–19 kDa outer membrane antigen are the major specific protective antigens of M. viscosa, and that the M. viscosa species consists of many different serotypes.
Significance and Impact of the Study: The results are important for the production of vaccines against winter ulcers and should also lead to better methods of verifying the bacteria and monitoring winter ulcers. |
| doi_str_mv | 10.1111/j.1365-2672.2007.03639.x |
| format | Article |
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Methods and Results: Lipooligosaccharides (LOS) and an approximately 17–19 kDa outer membrane antigen were shown to be the major specific antigens of M. viscosa. The size of the wall antigen differed between strain groups and even between strains reacting positively in the same sera. Four different serotypes of M. viscosa were determined by producing polyclonal sera. Western blot analysis revealed that sera from vaccinated fish groups that had good or fair protection reacted against the LOS and the 17/19 kDa antigen, while no antibody response was observed with sera from groups that showed no efficacy.
Conclusions: The study provides evidence that LOS and an approximately 17–19 kDa outer membrane antigen are the major specific protective antigens of M. viscosa, and that the M. viscosa species consists of many different serotypes.
Significance and Impact of the Study: The results are important for the production of vaccines against winter ulcers and should also lead to better methods of verifying the bacteria and monitoring winter ulcers.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2007.03639.x</identifier><identifier>PMID: 18194249</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Antibodies, Bacterial - immunology ; antigens ; Antigens, Bacterial - blood ; Biological and medical sciences ; Blotting, Western - methods ; Electrophoresis, Polyacrylamide Gel ; fish diseases ; Fish Diseases - immunology ; fish vaccines ; Fishes - immunology ; Fundamental and applied biological sciences. Psychology ; Immune Sera - immunology ; marine bacteria ; Microbiology ; Moritella - immunology ; Moritella viscosa ; Rabbits ; Serotyping ; Staining and Labeling ; Vaccines ; winter ulcer</subject><ispartof>Journal of applied microbiology, 2008-04, Vol.104 (4), p.944-951</ispartof><rights>2008 The Authors</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4479-aab3ada16e71cf62ac2485130f06e765ee456d9021765a982d52c1dbc96119803</citedby><cites>FETCH-LOGICAL-c4479-aab3ada16e71cf62ac2485130f06e765ee456d9021765a982d52c1dbc96119803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2007.03639.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2007.03639.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20243054$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18194249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heidarsdóttir, K.J.</creatorcontrib><creatorcontrib>Gravningen, K.</creatorcontrib><creatorcontrib>Benediktsdóttir, E.</creatorcontrib><title>Antigen profiles of the fish pathogen Moritella viscosa and protection in fish</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims: Moritella viscosa is a Gram‐negative bacterium that causes winter ulcer disease in salmonid fish cultured in sea water below 8°C. The aim of this study was to study the antigen profiles of these bacteria and to reveal the protection which the antigens induce in fish.
Methods and Results: Lipooligosaccharides (LOS) and an approximately 17–19 kDa outer membrane antigen were shown to be the major specific antigens of M. viscosa. The size of the wall antigen differed between strain groups and even between strains reacting positively in the same sera. Four different serotypes of M. viscosa were determined by producing polyclonal sera. Western blot analysis revealed that sera from vaccinated fish groups that had good or fair protection reacted against the LOS and the 17/19 kDa antigen, while no antibody response was observed with sera from groups that showed no efficacy.
Conclusions: The study provides evidence that LOS and an approximately 17–19 kDa outer membrane antigen are the major specific protective antigens of M. viscosa, and that the M. viscosa species consists of many different serotypes.
Significance and Impact of the Study: The results are important for the production of vaccines against winter ulcers and should also lead to better methods of verifying the bacteria and monitoring winter ulcers.</description><subject>Animals</subject><subject>Antibodies, Bacterial - immunology</subject><subject>antigens</subject><subject>Antigens, Bacterial - blood</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western - methods</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>fish diseases</subject><subject>Fish Diseases - immunology</subject><subject>fish vaccines</subject><subject>Fishes - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immune Sera - immunology</subject><subject>marine bacteria</subject><subject>Microbiology</subject><subject>Moritella - immunology</subject><subject>Moritella viscosa</subject><subject>Rabbits</subject><subject>Serotyping</subject><subject>Staining and Labeling</subject><subject>Vaccines</subject><subject>winter ulcer</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1PwyAYx4nRuDn9CoaL3lp5Ky0HD4vxNZte9EwYBcfSlVk63b69dGvmVS488Pwe-OcHAMQoxXHdLFJMeZYQnpOUIJSniHIq0s0RGB4ax7uaJRnKyQCchbBACFOU8VMwwAUWjDAxBK_junWfpoarxltXmQC9he3cQOvCHK5UO_ddd-ob15qqUvDbBe2Dgqouu5nW6Nb5Grp6N3EOTqyqgrno9xH4eLh_v3tKJm-Pz3fjSaIZy0Wi1IyqUmFucqwtJ0oTVmQxnUXximfGsIyXAhEcD0oUpMyIxuVMC46xKBAdgev9uzHC19qEVi5jri5gbfw6yBxRkeeERrDYg7rxITTGylXjlqrZSoxk51IuZKdMdspk51LuXMpNHL3s_1jPlqb8G-zlReCqB1TQqrKNqrULB44gwqJvFrnbPfcTBW__HUC-jKddRX8Bn86Ozw</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>Heidarsdóttir, K.J.</creator><creator>Gravningen, K.</creator><creator>Benediktsdóttir, E.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>Antigen profiles of the fish pathogen Moritella viscosa and protection in fish</title><author>Heidarsdóttir, K.J. ; Gravningen, K. ; Benediktsdóttir, E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4479-aab3ada16e71cf62ac2485130f06e765ee456d9021765a982d52c1dbc96119803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - immunology</topic><topic>antigens</topic><topic>Antigens, Bacterial - blood</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western - methods</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>fish diseases</topic><topic>Fish Diseases - immunology</topic><topic>fish vaccines</topic><topic>Fishes - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immune Sera - immunology</topic><topic>marine bacteria</topic><topic>Microbiology</topic><topic>Moritella - immunology</topic><topic>Moritella viscosa</topic><topic>Rabbits</topic><topic>Serotyping</topic><topic>Staining and Labeling</topic><topic>Vaccines</topic><topic>winter ulcer</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heidarsdóttir, K.J.</creatorcontrib><creatorcontrib>Gravningen, K.</creatorcontrib><creatorcontrib>Benediktsdóttir, E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heidarsdóttir, K.J.</au><au>Gravningen, K.</au><au>Benediktsdóttir, E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antigen profiles of the fish pathogen Moritella viscosa and protection in fish</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2008-04</date><risdate>2008</risdate><volume>104</volume><issue>4</issue><spage>944</spage><epage>951</epage><pages>944-951</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims: Moritella viscosa is a Gram‐negative bacterium that causes winter ulcer disease in salmonid fish cultured in sea water below 8°C. The aim of this study was to study the antigen profiles of these bacteria and to reveal the protection which the antigens induce in fish.
Methods and Results: Lipooligosaccharides (LOS) and an approximately 17–19 kDa outer membrane antigen were shown to be the major specific antigens of M. viscosa. The size of the wall antigen differed between strain groups and even between strains reacting positively in the same sera. Four different serotypes of M. viscosa were determined by producing polyclonal sera. Western blot analysis revealed that sera from vaccinated fish groups that had good or fair protection reacted against the LOS and the 17/19 kDa antigen, while no antibody response was observed with sera from groups that showed no efficacy.
Conclusions: The study provides evidence that LOS and an approximately 17–19 kDa outer membrane antigen are the major specific protective antigens of M. viscosa, and that the M. viscosa species consists of many different serotypes.
Significance and Impact of the Study: The results are important for the production of vaccines against winter ulcers and should also lead to better methods of verifying the bacteria and monitoring winter ulcers.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>18194249</pmid><doi>10.1111/j.1365-2672.2007.03639.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Antibodies, Bacterial - immunology antigens Antigens, Bacterial - blood Biological and medical sciences Blotting, Western - methods Electrophoresis, Polyacrylamide Gel fish diseases Fish Diseases - immunology fish vaccines Fishes - immunology Fundamental and applied biological sciences. Psychology Immune Sera - immunology marine bacteria Microbiology Moritella - immunology Moritella viscosa Rabbits Serotyping Staining and Labeling Vaccines winter ulcer |
| title | Antigen profiles of the fish pathogen Moritella viscosa and protection in fish |
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