Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni
A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of Schistosoma mansoni. Serum samples were obtained from individuals with acute ( n = 23) and chronic ( n = 30) schistosomiasis, with other parasitoses ( n = 39) or without parasitic infec...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 2008-04, Vol.102 (4), p.360-366 |
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| creator | de Oliveira, Edward José Kanamura, Hermínia Yohko Takei, Kioko Hirata, Rosario Dominguez Crespo Valli, Luiz Carlos Pedrosa Nguyen, Nga Yen de Carvalho Rodrigues, Izabel de Jesus, Amélia Ribeiro Hirata, Mário Hiroyuki |
| description | A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of
Schistosoma mansoni. Serum samples were obtained from individuals with acute (
n
=
23) and chronic (
n
=
30) schistosomiasis, with other parasitoses (
n
=
39) or without parasitic infections (
n
=
100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in κ concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (
P
=
0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni. |
| doi_str_mv | 10.1016/j.trstmh.2007.11.008 |
| format | Article |
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Schistosoma mansoni. Serum samples were obtained from individuals with acute (
n
=
23) and chronic (
n
=
30) schistosomiasis, with other parasitoses (
n
=
39) or without parasitic infections (
n
=
100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in κ concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (
P
=
0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1016/j.trstmh.2007.11.008</identifier><identifier>PMID: 18314149</identifier><identifier>CODEN: TRSTAZ</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Acute Disease ; Animals ; Antibodies, Protozoan - blood ; Antigens, Helminth - immunology ; Biological and medical sciences ; Chronic Disease ; Diagnosis ; Diseases caused by trematodes ; ELISA ; Enzyme-Linked Immunosorbent Assay - methods ; General aspects ; Helminthic diseases ; Humans ; Immunoglobulin G - blood ; Immunoglobulin M - blood ; Infectious diseases ; Medical sciences ; Parasitic diseases ; Peptide Fragments - immunology ; Schistosoma mansoni ; Schistosoma mansoni - immunology ; Schistosomiases ; Schistosomiasis mansoni - diagnosis ; Sensitivity and Specificity ; Serology ; Synthetic peptides</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 2008-04, Vol.102 (4), p.360-366</ispartof><rights>2007 Royal Society of Tropical Medicine and Hygiene</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-7e710ba57e3f4f860c6a7e164bc3dfe2ae1d64695f87498495d58ec6bf170b823</citedby><cites>FETCH-LOGICAL-c343t-7e710ba57e3f4f860c6a7e164bc3dfe2ae1d64695f87498495d58ec6bf170b823</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20199775$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18314149$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Oliveira, Edward José</creatorcontrib><creatorcontrib>Kanamura, Hermínia Yohko</creatorcontrib><creatorcontrib>Takei, Kioko</creatorcontrib><creatorcontrib>Hirata, Rosario Dominguez Crespo</creatorcontrib><creatorcontrib>Valli, Luiz Carlos Pedrosa</creatorcontrib><creatorcontrib>Nguyen, Nga Yen</creatorcontrib><creatorcontrib>de Carvalho Rodrigues, Izabel</creatorcontrib><creatorcontrib>de Jesus, Amélia Ribeiro</creatorcontrib><creatorcontrib>Hirata, Mário Hiroyuki</creatorcontrib><title>Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of
Schistosoma mansoni. Serum samples were obtained from individuals with acute (
n
=
23) and chronic (
n
=
30) schistosomiasis, with other parasitoses (
n
=
39) or without parasitic infections (
n
=
100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in κ concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (
P
=
0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni.</description><subject>Acute Disease</subject><subject>Animals</subject><subject>Antibodies, Protozoan - blood</subject><subject>Antigens, Helminth - immunology</subject><subject>Biological and medical sciences</subject><subject>Chronic Disease</subject><subject>Diagnosis</subject><subject>Diseases caused by trematodes</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>General aspects</subject><subject>Helminthic diseases</subject><subject>Humans</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin M - blood</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>Peptide Fragments - immunology</subject><subject>Schistosoma mansoni</subject><subject>Schistosoma mansoni - immunology</subject><subject>Schistosomiases</subject><subject>Schistosomiasis mansoni - diagnosis</subject><subject>Sensitivity and Specificity</subject><subject>Serology</subject><subject>Synthetic peptides</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kFGL1DAQx4Mo3nr6DUT6om-tSZM07YtwHOfd4R4Kp3D4EtJ0upu1bdZMVm6_vSldzjdhYGD-vxmGHyFvGS0YZdXHXREDxnFblJSqgrGC0voZWbFa1TmXlD8nK0q5zJuS8jPyCnFHaSmZbF6SM1ZzJphoVmRzf5ziFqKz2R720XWAmUk1pYpuA1MKWoOQuXmSXa1v7y-y3odsMK0PJvpwzDpnNpNHh5nvM7Rbh9GjH52ZR6OZ0E_uNXnRmwHhzamfkx-fr75f3uTrr9e3lxfr3HLBY65AMdoaqYD3oq8raiujgFWitbzroTTAukpUjexrJZpaNLKTNdiq7ZmibV3yc_JhubsP_vcBMOrRoYVhMBP4A2pFecM4m0GxgDZ4xAC93gc3mnDUjOpZsN7pRbCeBWvGdBKc1t6d7h_aEbp_SyejCXh_AgxaM_TBTNbhE1dS1jRKycTlC5dsweNTbsIvXSmupL55-Kkf7tSafhHf9F3iPy08JHt_HASN1sFkoXMBbNSdd____C_bCquO</recordid><startdate>200804</startdate><enddate>200804</enddate><creator>de Oliveira, Edward José</creator><creator>Kanamura, Hermínia Yohko</creator><creator>Takei, Kioko</creator><creator>Hirata, Rosario Dominguez Crespo</creator><creator>Valli, Luiz Carlos Pedrosa</creator><creator>Nguyen, Nga Yen</creator><creator>de Carvalho Rodrigues, Izabel</creator><creator>de Jesus, Amélia Ribeiro</creator><creator>Hirata, Mário Hiroyuki</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><general>Elsevier</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200804</creationdate><title>Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni</title><author>de Oliveira, Edward José ; Kanamura, Hermínia Yohko ; Takei, Kioko ; Hirata, Rosario Dominguez Crespo ; Valli, Luiz Carlos Pedrosa ; Nguyen, Nga Yen ; de Carvalho Rodrigues, Izabel ; de Jesus, Amélia Ribeiro ; Hirata, Mário Hiroyuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-7e710ba57e3f4f860c6a7e164bc3dfe2ae1d64695f87498495d58ec6bf170b823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acute Disease</topic><topic>Animals</topic><topic>Antibodies, Protozoan - blood</topic><topic>Antigens, Helminth - immunology</topic><topic>Biological and medical sciences</topic><topic>Chronic Disease</topic><topic>Diagnosis</topic><topic>Diseases caused by trematodes</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>General aspects</topic><topic>Helminthic diseases</topic><topic>Humans</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin M - blood</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>Peptide Fragments - immunology</topic><topic>Schistosoma mansoni</topic><topic>Schistosoma mansoni - immunology</topic><topic>Schistosomiases</topic><topic>Schistosomiasis mansoni - diagnosis</topic><topic>Sensitivity and Specificity</topic><topic>Serology</topic><topic>Synthetic peptides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira, Edward José</creatorcontrib><creatorcontrib>Kanamura, Hermínia Yohko</creatorcontrib><creatorcontrib>Takei, Kioko</creatorcontrib><creatorcontrib>Hirata, Rosario Dominguez Crespo</creatorcontrib><creatorcontrib>Valli, Luiz Carlos Pedrosa</creatorcontrib><creatorcontrib>Nguyen, Nga Yen</creatorcontrib><creatorcontrib>de Carvalho Rodrigues, Izabel</creatorcontrib><creatorcontrib>de Jesus, Amélia Ribeiro</creatorcontrib><creatorcontrib>Hirata, Mário Hiroyuki</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Oliveira, Edward José</au><au>Kanamura, Hermínia Yohko</au><au>Takei, Kioko</au><au>Hirata, Rosario Dominguez Crespo</au><au>Valli, Luiz Carlos Pedrosa</au><au>Nguyen, Nga Yen</au><au>de Carvalho Rodrigues, Izabel</au><au>de Jesus, Amélia Ribeiro</au><au>Hirata, Mário Hiroyuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>2008-04</date><risdate>2008</risdate><volume>102</volume><issue>4</issue><spage>360</spage><epage>366</epage><pages>360-366</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><coden>TRSTAZ</coden><abstract>A pool of five synthetic peptides was used as an antigenic base in an ELISA (ELISA-Pp) for laboratory diagnosis of
Schistosoma mansoni. Serum samples were obtained from individuals with acute (
n
=
23) and chronic (
n
=
30) schistosomiasis, with other parasitoses (
n
=
39) or without parasitic infections (
n
=
100). ELISA-Pp was compared with other immunoenzymatic methods for detection of IgM (IgM-ELISA) or IgG (IgG-ELISA) as well as an immunofluorescence test for detection of IgM antibodies (IgM-IFT). The sensitivity and specificity of ELISA-Pp was 86.8% and 94.2% when tested on the schistosomiasis group and the non-schistosomiasis group, respectively. Comparison of ELISA-Pp with other serological methods resulted in κ concordance indices varying from 0.59 to 0.75. Evaluation of anti-peptide IgG antibodies showed higher levels in patients with acute compared with chronic schistosomiasis (
P
=
0.001). ELISA-Pp showed satisfactory sensitivity and high specificity and may constitute a potentially useful method for laboratory diagnosis of schistosomiasis mansoni.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18314149</pmid><doi>10.1016/j.trstmh.2007.11.008</doi><tpages>7</tpages></addata></record> |
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| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 2008-04, Vol.102 (4), p.360-366 |
| issn | 0035-9203 1878-3503 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Acute Disease Animals Antibodies, Protozoan - blood Antigens, Helminth - immunology Biological and medical sciences Chronic Disease Diagnosis Diseases caused by trematodes ELISA Enzyme-Linked Immunosorbent Assay - methods General aspects Helminthic diseases Humans Immunoglobulin G - blood Immunoglobulin M - blood Infectious diseases Medical sciences Parasitic diseases Peptide Fragments - immunology Schistosoma mansoni Schistosoma mansoni - immunology Schistosomiases Schistosomiasis mansoni - diagnosis Sensitivity and Specificity Serology Synthetic peptides |
| title | Synthetic peptides as an antigenic base in an ELISA for laboratory diagnosis of schistosomiasis mansoni |
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