Cryptococcal phospholipase B antigen is not detected in serum of patients infected with Cryptococcus neoformans using a sandwich enzyme-linked immunosorbent assay
Abstract Extracellular phospholipase B (PLB) is a virulence determinant of Cryptococcus neoformans and Cryptococcus gattii. In this study, we developed a sensitive enzyme-linked immunosorbent assay (ELISA) for PLB antigen with a detection limit of 3.9 ng mL−1. PLB was detected in culture supernatant...
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| Veröffentlicht in: | FEMS yeast research 2007-05, Vol.7 (3), p.465-470 |
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| creator | Wu, Qi Xuan Chen, Sharon C. A. Santangelo, Rosemary T. Martin, Patricia Malik, Richard Sorrell, Tania C. |
| description | Abstract
Extracellular phospholipase B (PLB) is a virulence determinant of Cryptococcus neoformans and Cryptococcus gattii. In this study, we developed a sensitive enzyme-linked immunosorbent assay (ELISA) for PLB antigen with a detection limit of 3.9 ng mL−1. PLB was detected in culture supernatants of C. neoformans and C. gattii. PLB, however, was not detected in sera of seven human patients and 10 feline patients with active cryptococcosis. Furthermore, none of five rats with extensive pulmonary C. gattii infection had a positive ELISA test result. In conclusion, cryptococcal PLB could not be detected in serum using a PLB antigen-based ELISA. Despite its sensitivity, this ELISA is of limited diagnostic value. Exploration of further extracellular molecules suitable for serodiagnosis of active cryptococcal infection is warranted. |
| doi_str_mv | 10.1111/j.1567-1364.2006.00187.x |
| format | Article |
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Extracellular phospholipase B (PLB) is a virulence determinant of Cryptococcus neoformans and Cryptococcus gattii. In this study, we developed a sensitive enzyme-linked immunosorbent assay (ELISA) for PLB antigen with a detection limit of 3.9 ng mL−1. PLB was detected in culture supernatants of C. neoformans and C. gattii. PLB, however, was not detected in sera of seven human patients and 10 feline patients with active cryptococcosis. Furthermore, none of five rats with extensive pulmonary C. gattii infection had a positive ELISA test result. In conclusion, cryptococcal PLB could not be detected in serum using a PLB antigen-based ELISA. Despite its sensitivity, this ELISA is of limited diagnostic value. Exploration of further extracellular molecules suitable for serodiagnosis of active cryptococcal infection is warranted.</description><identifier>ISSN: 1567-1356</identifier><identifier>EISSN: 1567-1364</identifier><identifier>DOI: 10.1111/j.1567-1364.2006.00187.x</identifier><identifier>PMID: 17266733</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Antigens ; Cats ; Cryptococcosis ; Cryptococcosis - blood ; Cryptococcosis - microbiology ; Cryptococcus ; Cryptococcus neoformans ; Cryptococcus neoformans - enzymology ; ELISA ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzymes ; Fungal infections ; Humans ; Immunoglobulin G - chemistry ; Lysophospholipase - blood ; Phospholipase ; phospholipase B ; Rats ; Sensitivity and Specificity ; Virulence</subject><ispartof>FEMS yeast research, 2007-05, Vol.7 (3), p.465-470</ispartof><rights>2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved 2006</rights><rights>2006 Federation of European Microbiological Societies Published by Blackwell Publishing Ltd. All rights reserved</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4387-c994c28cebdff0e3c889d7c4e09f7abfce73869b43546cd454de6ab7fcc73f713</citedby><cites>FETCH-LOGICAL-c4387-c994c28cebdff0e3c889d7c4e09f7abfce73869b43546cd454de6ab7fcc73f713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1567-1364.2006.00187.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1567-1364.2006.00187.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17266733$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Qi Xuan</creatorcontrib><creatorcontrib>Chen, Sharon C. A.</creatorcontrib><creatorcontrib>Santangelo, Rosemary T.</creatorcontrib><creatorcontrib>Martin, Patricia</creatorcontrib><creatorcontrib>Malik, Richard</creatorcontrib><creatorcontrib>Sorrell, Tania C.</creatorcontrib><title>Cryptococcal phospholipase B antigen is not detected in serum of patients infected with Cryptococcus neoformans using a sandwich enzyme-linked immunosorbent assay</title><title>FEMS yeast research</title><addtitle>FEMS Yeast Res</addtitle><description>Abstract
Extracellular phospholipase B (PLB) is a virulence determinant of Cryptococcus neoformans and Cryptococcus gattii. In this study, we developed a sensitive enzyme-linked immunosorbent assay (ELISA) for PLB antigen with a detection limit of 3.9 ng mL−1. PLB was detected in culture supernatants of C. neoformans and C. gattii. PLB, however, was not detected in sera of seven human patients and 10 feline patients with active cryptococcosis. Furthermore, none of five rats with extensive pulmonary C. gattii infection had a positive ELISA test result. In conclusion, cryptococcal PLB could not be detected in serum using a PLB antigen-based ELISA. Despite its sensitivity, this ELISA is of limited diagnostic value. Exploration of further extracellular molecules suitable for serodiagnosis of active cryptococcal infection is warranted.</description><subject>Animals</subject><subject>Antigens</subject><subject>Cats</subject><subject>Cryptococcosis</subject><subject>Cryptococcosis - blood</subject><subject>Cryptococcosis - microbiology</subject><subject>Cryptococcus</subject><subject>Cryptococcus neoformans</subject><subject>Cryptococcus neoformans - enzymology</subject><subject>ELISA</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzymes</subject><subject>Fungal infections</subject><subject>Humans</subject><subject>Immunoglobulin G - chemistry</subject><subject>Lysophospholipase - blood</subject><subject>Phospholipase</subject><subject>phospholipase B</subject><subject>Rats</subject><subject>Sensitivity and Specificity</subject><subject>Virulence</subject><issn>1567-1356</issn><issn>1567-1364</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqNkVGL1DAUhYso7rr6FyQg-NaaNm2Tgi86uCosCKIPPoU0vdnJ2CY1t2W2_hx_qel22AVFMBAScr9zcuAkCclplsf16pDlVc3TnNVlVlBaZ5Tmgmc3D5Lzu8HDu3tVnyVPEA8R4pSKx8lZzou65oydJ792YRknr73Wqifj3mPcvR0VAnlLlJvsNThikTg_kQ4m0BN0xDqCEOaBeENGNVlwE8ZHs02PdtqTe985isEbHwblkMxo3TVRBJXrjlbvCbifywBpb9331XkYZufRhzZ6EoWolqfJI6N6hGen8yL5evnuy-5DevXp_cfdm6tUl0zwVDdNqQuhoe2MocC0EE3HdQm0MVy1RgNnom7aklVlrbuyKjuoVcuN1pwZnrOL5OXmOwb_Ywac5GBRQ9-rGH9GySkTVVWu4Is_wIOfg4vZZMFYVVDeCB4psVE6eMQARo7BDiosMqdybVEe5FqQXMuSa4vytkV5E6XPTx_M7QDdvfBUWwReb8DR9rD8t7G8_PY5v43GNrmfx3-I079T_QYi_L8j</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Wu, Qi Xuan</creator><creator>Chen, Sharon C. A.</creator><creator>Santangelo, Rosemary T.</creator><creator>Martin, Patricia</creator><creator>Malik, Richard</creator><creator>Sorrell, Tania C.</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20070501</creationdate><title>Cryptococcal phospholipase B antigen is not detected in serum of patients infected with Cryptococcus neoformans using a sandwich enzyme-linked immunosorbent assay</title><author>Wu, Qi Xuan ; Chen, Sharon C. A. ; Santangelo, Rosemary T. ; Martin, Patricia ; Malik, Richard ; Sorrell, Tania C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4387-c994c28cebdff0e3c889d7c4e09f7abfce73869b43546cd454de6ab7fcc73f713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Cats</topic><topic>Cryptococcosis</topic><topic>Cryptococcosis - blood</topic><topic>Cryptococcosis - microbiology</topic><topic>Cryptococcus</topic><topic>Cryptococcus neoformans</topic><topic>Cryptococcus neoformans - enzymology</topic><topic>ELISA</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzymes</topic><topic>Fungal infections</topic><topic>Humans</topic><topic>Immunoglobulin G - chemistry</topic><topic>Lysophospholipase - blood</topic><topic>Phospholipase</topic><topic>phospholipase B</topic><topic>Rats</topic><topic>Sensitivity and Specificity</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Qi Xuan</creatorcontrib><creatorcontrib>Chen, Sharon C. A.</creatorcontrib><creatorcontrib>Santangelo, Rosemary T.</creatorcontrib><creatorcontrib>Martin, Patricia</creatorcontrib><creatorcontrib>Malik, Richard</creatorcontrib><creatorcontrib>Sorrell, Tania C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS yeast research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Qi Xuan</au><au>Chen, Sharon C. A.</au><au>Santangelo, Rosemary T.</au><au>Martin, Patricia</au><au>Malik, Richard</au><au>Sorrell, Tania C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryptococcal phospholipase B antigen is not detected in serum of patients infected with Cryptococcus neoformans using a sandwich enzyme-linked immunosorbent assay</atitle><jtitle>FEMS yeast research</jtitle><addtitle>FEMS Yeast Res</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>7</volume><issue>3</issue><spage>465</spage><epage>470</epage><pages>465-470</pages><issn>1567-1356</issn><eissn>1567-1364</eissn><abstract>Abstract
Extracellular phospholipase B (PLB) is a virulence determinant of Cryptococcus neoformans and Cryptococcus gattii. In this study, we developed a sensitive enzyme-linked immunosorbent assay (ELISA) for PLB antigen with a detection limit of 3.9 ng mL−1. PLB was detected in culture supernatants of C. neoformans and C. gattii. PLB, however, was not detected in sera of seven human patients and 10 feline patients with active cryptococcosis. Furthermore, none of five rats with extensive pulmonary C. gattii infection had a positive ELISA test result. In conclusion, cryptococcal PLB could not be detected in serum using a PLB antigen-based ELISA. Despite its sensitivity, this ELISA is of limited diagnostic value. Exploration of further extracellular molecules suitable for serodiagnosis of active cryptococcal infection is warranted.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17266733</pmid><doi>10.1111/j.1567-1364.2006.00187.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | FEMS yeast research, 2007-05, Vol.7 (3), p.465-470 |
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| source | Oxford Journals Open Access Collection; MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Animals Antigens Cats Cryptococcosis Cryptococcosis - blood Cryptococcosis - microbiology Cryptococcus Cryptococcus neoformans Cryptococcus neoformans - enzymology ELISA Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzymes Fungal infections Humans Immunoglobulin G - chemistry Lysophospholipase - blood Phospholipase phospholipase B Rats Sensitivity and Specificity Virulence |
| title | Cryptococcal phospholipase B antigen is not detected in serum of patients infected with Cryptococcus neoformans using a sandwich enzyme-linked immunosorbent assay |
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