Implementation of a bimorph-based aperture tapping-SNOM with an incubator to study the evolution of cultured living cells

We present the implementation of a tapping-mode aperture scanning near-field optical microscope (Tapping-SNOM) to a Binder CB incubator (Istituto di Struttura della Materia, Rome, Italy). The microscope operates in the intermittent contact mode using a nonbent optical fibre allowing to reduce the pe...

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Veröffentlicht in:Journal of microscopy (Oxford) 2008-03, Vol.229 (3), p.433-439
Hauptverfasser: LONGO, G, GIRASOLE, M, CRICENTI, A
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creator LONGO, G
GIRASOLE, M
CRICENTI, A
description We present the implementation of a tapping-mode aperture scanning near-field optical microscope (Tapping-SNOM) to a Binder CB incubator (Istituto di Struttura della Materia, Rome, Italy). The microscope operates in the intermittent contact mode using a nonbent optical fibre allowing to reduce the perturbation exerted on the sample, while the incubator maintains a constant temperature, humidity and CO₂ level. This instrument can maintain and analyse in a controlled environment different samples, both organic and nonorganic. In particular, the Tapping-SNOM can study different cell lines at nanometric resolution and in physiological buffer, following the evolution of the living cells almost indefinitely. We will present several examples of the capabilities of the tapping scanning near-field optical microscope in the study of different lines of living cells, showing corresponding topographical, optical or phase-lag images of the live samples, evidencing the excellent stability, versatility and resolution of the system.
doi_str_mv 10.1111/j.1365-2818.2008.01924.x
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source Wiley-Blackwell Journals; Wiley Online Library website; MEDLINE
subjects Animals
Cell Line, Tumor - ultrastructure
Cells, Cultured - ultrastructure
Endothelial Cells - physiology
Endothelial Cells - ultrastructure
Equipment Design
Erythrocytes - physiology
Erythrocytes - ultrastructure
Fiber Optic Technology
Humans
Incubator
Keratinocytes - physiology
Keratinocytes - ultrastructure
living cells
Microscopy, Electron, Scanning - instrumentation
Microscopy, Electron, Scanning - methods
Microscopy, Scanning Probe - instrumentation
Neuroblastoma
Q-factor
SNOM
Super resolution
Swine
tapping-mode
title Implementation of a bimorph-based aperture tapping-SNOM with an incubator to study the evolution of cultured living cells
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