Isolation of Murine Hair-Inducing Cells Using the Cell Surface Marker Prominin-1/CD133
Hair is a mini-organ in which dermal papilla (DP) cells play important roles in hair follicle morphogenesis and formation via interactions with epithelial cells. DP cells have previously been difficult to analyze because of the lack of a specific surface marker. We have demonstrated that prominin-1/...
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| Veröffentlicht in: | Journal of investigative dermatology 2007-05, Vol.127 (5), p.1052-1060 |
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| container_title | Journal of investigative dermatology |
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| creator | Ito, Yuriko Hamazaki, Tatsuo S. Ohnuma, Kiyoshi Tamaki, Kunihiko Asashima, Makoto Okochi, Hitoshi |
| description | Hair is a mini-organ in which dermal papilla (DP) cells play important roles in hair follicle morphogenesis and formation via interactions with epithelial cells. DP cells have previously been difficult to analyze because of the lack of a specific surface marker. We have demonstrated that prominin-1/CD133 (CD133) is a useful marker for murine DP cells. DP cells express CD133 during the early anagen stage (active growth phase) not only during hair morphogenesis, but also during the growth phase of hairs after birth. Gene expression and flow cytometric analysis revealed that CD133-positive (+) cells in the skin possess the characteristics of DP cells. The CD133(+) cells isolated from embryonic or adult skin-induced new hair follicles in vivo when they were transplanted into nude mice mixed with embryonic epithelial cells, but CD133-negative(−) cells could not. We propose that the CD133 is a novel surface marker useful for collecting DP cells in the anagen stage and for analyzing the function of DP. |
| doi_str_mv | 10.1038/sj.jid.5700665 |
| format | Article |
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DP cells have previously been difficult to analyze because of the lack of a specific surface marker. We have demonstrated that prominin-1/CD133 (CD133) is a useful marker for murine DP cells. DP cells express CD133 during the early anagen stage (active growth phase) not only during hair morphogenesis, but also during the growth phase of hairs after birth. Gene expression and flow cytometric analysis revealed that CD133-positive (+) cells in the skin possess the characteristics of DP cells. The CD133(+) cells isolated from embryonic or adult skin-induced new hair follicles in vivo when they were transplanted into nude mice mixed with embryonic epithelial cells, but CD133-negative(−) cells could not. We propose that the CD133 is a novel surface marker useful for collecting DP cells in the anagen stage and for analyzing the function of DP.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1038/sj.jid.5700665</identifier><identifier>PMID: 17185982</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>AC133 Antigen ; Animals ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Biological and medical sciences ; Biomarkers - metabolism ; Dermatology ; Dermis - cytology ; Dermis - metabolism ; Epithelial Cells - cytology ; Epithelial Cells - metabolism ; Gene Expression Regulation ; Gene Expression Regulation, Developmental ; Glycoproteins - genetics ; Glycoproteins - metabolism ; Hair - cytology ; Hair - metabolism ; Hair Follicle - embryology ; Hair Follicle - growth & development ; Hair Follicle - metabolism ; Medical sciences ; Membrane Proteins ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Nude ; Mice, Transgenic ; Peptides - genetics ; Peptides - metabolism ; Versicans - metabolism</subject><ispartof>Journal of investigative dermatology, 2007-05, Vol.127 (5), p.1052-1060</ispartof><rights>2007 The Society for Investigative Dermatology, Inc</rights><rights>2007 INIST-CNRS</rights><rights>Copyright Nature Publishing Group May 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c505t-3ba95cec5a5aa09cd8e57b8b9d50f7217db2382da65b09f52151b448f9a3f67e3</citedby><cites>FETCH-LOGICAL-c505t-3ba95cec5a5aa09cd8e57b8b9d50f7217db2382da65b09f52151b448f9a3f67e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19046270$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17185982$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ito, Yuriko</creatorcontrib><creatorcontrib>Hamazaki, Tatsuo S.</creatorcontrib><creatorcontrib>Ohnuma, Kiyoshi</creatorcontrib><creatorcontrib>Tamaki, Kunihiko</creatorcontrib><creatorcontrib>Asashima, Makoto</creatorcontrib><creatorcontrib>Okochi, Hitoshi</creatorcontrib><title>Isolation of Murine Hair-Inducing Cells Using the Cell Surface Marker Prominin-1/CD133</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Hair is a mini-organ in which dermal papilla (DP) cells play important roles in hair follicle morphogenesis and formation via interactions with epithelial cells. DP cells have previously been difficult to analyze because of the lack of a specific surface marker. We have demonstrated that prominin-1/CD133 (CD133) is a useful marker for murine DP cells. DP cells express CD133 during the early anagen stage (active growth phase) not only during hair morphogenesis, but also during the growth phase of hairs after birth. Gene expression and flow cytometric analysis revealed that CD133-positive (+) cells in the skin possess the characteristics of DP cells. The CD133(+) cells isolated from embryonic or adult skin-induced new hair follicles in vivo when they were transplanted into nude mice mixed with embryonic epithelial cells, but CD133-negative(−) cells could not. We propose that the CD133 is a novel surface marker useful for collecting DP cells in the anagen stage and for analyzing the function of DP.</description><subject>AC133 Antigen</subject><subject>Animals</subject><subject>Antigens, CD - genetics</subject><subject>Antigens, CD - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Dermatology</subject><subject>Dermis - cytology</subject><subject>Dermis - metabolism</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - metabolism</subject><subject>Hair - cytology</subject><subject>Hair - metabolism</subject><subject>Hair Follicle - embryology</subject><subject>Hair Follicle - growth & development</subject><subject>Hair Follicle - metabolism</subject><subject>Medical sciences</subject><subject>Membrane Proteins</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Nude</subject><subject>Mice, Transgenic</subject><subject>Peptides - genetics</subject><subject>Peptides - metabolism</subject><subject>Versicans - metabolism</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kMFLHDEUh0Ox1HXba48yCHqbNclMJpljWbUuKBaq0lvIJC8109lEkxnB_76xOyAUPL334Hs_fnwIfSV4RXAlTlO_6p1ZMY5x07APaEEYrUrCa76HFhhTWlJMf-2jg5R6jElTM_EJ7RNOBGsFXaD7TQqDGl3wRbDF9RSdh-JSuVhuvJm087-LNQxDKu7S6z4-wL-7-DlFqzQU1yr-gVj8iGHrvPMlOV2fkar6jD5aNST4Ms8lurs4v11fllc33zfrb1elZpiNZdWplmnQTDGlcKuNAMY70bWGYcsp4aajlaBGNazDrWWUMNLVtbCtqmzDoVqik13uYwxPE6RRbl3SuaDyEKYkeXbEKG0yePQf2Icp-txN0iySN6LmGVrtIB1DShGsfIxuq-KLJFi-6papl1m3nHXnh8M5deq2YN7w2W8GjmdAJa0GG5XXLr1xLa4bmksukdhxkGU9O4gyaQdeg3ER9ChNcO91-AtTp5od</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Ito, Yuriko</creator><creator>Hamazaki, Tatsuo S.</creator><creator>Ohnuma, Kiyoshi</creator><creator>Tamaki, Kunihiko</creator><creator>Asashima, Makoto</creator><creator>Okochi, Hitoshi</creator><general>Elsevier Inc</general><general>Nature Publishing</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070501</creationdate><title>Isolation of Murine Hair-Inducing Cells Using the Cell Surface Marker Prominin-1/CD133</title><author>Ito, Yuriko ; Hamazaki, Tatsuo S. ; Ohnuma, Kiyoshi ; Tamaki, Kunihiko ; Asashima, Makoto ; Okochi, Hitoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c505t-3ba95cec5a5aa09cd8e57b8b9d50f7217db2382da65b09f52151b448f9a3f67e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>AC133 Antigen</topic><topic>Animals</topic><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Dermatology</topic><topic>Dermis - cytology</topic><topic>Dermis - metabolism</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - metabolism</topic><topic>Hair - cytology</topic><topic>Hair - metabolism</topic><topic>Hair Follicle - embryology</topic><topic>Hair Follicle - growth & development</topic><topic>Hair Follicle - metabolism</topic><topic>Medical sciences</topic><topic>Membrane Proteins</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Nude</topic><topic>Mice, Transgenic</topic><topic>Peptides - genetics</topic><topic>Peptides - metabolism</topic><topic>Versicans - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ito, Yuriko</creatorcontrib><creatorcontrib>Hamazaki, Tatsuo S.</creatorcontrib><creatorcontrib>Ohnuma, Kiyoshi</creatorcontrib><creatorcontrib>Tamaki, Kunihiko</creatorcontrib><creatorcontrib>Asashima, Makoto</creatorcontrib><creatorcontrib>Okochi, Hitoshi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ito, Yuriko</au><au>Hamazaki, Tatsuo S.</au><au>Ohnuma, Kiyoshi</au><au>Tamaki, Kunihiko</au><au>Asashima, Makoto</au><au>Okochi, Hitoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Murine Hair-Inducing Cells Using the Cell Surface Marker Prominin-1/CD133</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>127</volume><issue>5</issue><spage>1052</spage><epage>1060</epage><pages>1052-1060</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>Hair is a mini-organ in which dermal papilla (DP) cells play important roles in hair follicle morphogenesis and formation via interactions with epithelial cells. DP cells have previously been difficult to analyze because of the lack of a specific surface marker. We have demonstrated that prominin-1/CD133 (CD133) is a useful marker for murine DP cells. DP cells express CD133 during the early anagen stage (active growth phase) not only during hair morphogenesis, but also during the growth phase of hairs after birth. Gene expression and flow cytometric analysis revealed that CD133-positive (+) cells in the skin possess the characteristics of DP cells. The CD133(+) cells isolated from embryonic or adult skin-induced new hair follicles in vivo when they were transplanted into nude mice mixed with embryonic epithelial cells, but CD133-negative(−) cells could not. We propose that the CD133 is a novel surface marker useful for collecting DP cells in the anagen stage and for analyzing the function of DP.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>17185982</pmid><doi>10.1038/sj.jid.5700665</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | AC133 Antigen Animals Antigens, CD - genetics Antigens, CD - metabolism Biological and medical sciences Biomarkers - metabolism Dermatology Dermis - cytology Dermis - metabolism Epithelial Cells - cytology Epithelial Cells - metabolism Gene Expression Regulation Gene Expression Regulation, Developmental Glycoproteins - genetics Glycoproteins - metabolism Hair - cytology Hair - metabolism Hair Follicle - embryology Hair Follicle - growth & development Hair Follicle - metabolism Medical sciences Membrane Proteins Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Nude Mice, Transgenic Peptides - genetics Peptides - metabolism Versicans - metabolism |
| title | Isolation of Murine Hair-Inducing Cells Using the Cell Surface Marker Prominin-1/CD133 |
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