Anticancer Effect of a New Benzophenanthridine Isolated from Zanthoxylum madagascariense (Rutaceline)
Fractionation of the cyclohexane extract from the stem bark powder of Zanthoxylum madagascariense led to the isolation of a new benzophenanthridine-type alkaloid, hydrochloride of 2,3-methylendioxy-8-hydroxy-7-methoxy-benzo[C]phenanthridine (Rutaceline), characterized on the basis of its spectral da...
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| Veröffentlicht in: | In vivo (Athens) 2007-03, Vol.21 (2), p.417-422 |
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| creator | Pachon, G Rasoanaivo, H Azqueta, A Rakotozafy, J C Raharisololalao, A De Cerain, A López De Lapuente, J Borràs, M Moukha, S Centelles, J J Creppy, E E Cascante, M |
| description | Fractionation of the cyclohexane extract from the stem bark powder of Zanthoxylum madagascariense led to the isolation of
a new benzophenanthridine-type alkaloid, hydrochloride of 2,3-methylendioxy-8-hydroxy-7-methoxy-benzo[C]phenanthridine (Rutaceline),
characterized on the basis of its spectral data. Rutaceline was evaluated for its antiproliferative capacity on the human
colorectal adenocarcinoma (Caco-2) and the African green monkey kidney (Vero) cell lines. The 50% inhibition of cell growth
(IC 50 ) obtained after 24 h incubation was similar for both cells lines (110-115 μg/ml, i.e. 269-281 μM), but at 48 h the IC 50 value for the Caco-2 cells was lower than for the Vero cells (20 μg/ml, i.e. 49 μM versus 90 μg/ml, i.e. 220 μM) indicating
a higher cell growth inhibitory effect on the colon adenocarcinoma cells. At the respective IC 50 concentrations, Rutaceline did not significantly induce apoptosis but induced cell cycle arrest in the G0/G1 phase, as well
as a decrease of cells in S phase. Rutaceline also induced DNA fragmentation in both cell lines, as revealed by agarose gel
electrophoresis, and a dose-dependent clastogenic effect in both cell lines as revealed by the Comet assay. |
| format | Article |
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a new benzophenanthridine-type alkaloid, hydrochloride of 2,3-methylendioxy-8-hydroxy-7-methoxy-benzo[C]phenanthridine (Rutaceline),
characterized on the basis of its spectral data. Rutaceline was evaluated for its antiproliferative capacity on the human
colorectal adenocarcinoma (Caco-2) and the African green monkey kidney (Vero) cell lines. The 50% inhibition of cell growth
(IC 50 ) obtained after 24 h incubation was similar for both cells lines (110-115 μg/ml, i.e. 269-281 μM), but at 48 h the IC 50 value for the Caco-2 cells was lower than for the Vero cells (20 μg/ml, i.e. 49 μM versus 90 μg/ml, i.e. 220 μM) indicating
a higher cell growth inhibitory effect on the colon adenocarcinoma cells. At the respective IC 50 concentrations, Rutaceline did not significantly induce apoptosis but induced cell cycle arrest in the G0/G1 phase, as well
as a decrease of cells in S phase. Rutaceline also induced DNA fragmentation in both cell lines, as revealed by agarose gel
electrophoresis, and a dose-dependent clastogenic effect in both cell lines as revealed by the Comet assay.</description><identifier>ISSN: 0258-851X</identifier><identifier>EISSN: 1791-7549</identifier><identifier>PMID: 17436597</identifier><language>eng</language><publisher>Greece: International Institute of Anticancer Research</publisher><subject>Adenocarcinoma ; Animals ; Antineoplastic Agents - chemistry ; Antineoplastic Agents - isolation & purification ; Antineoplastic Agents - pharmacology ; Benzophenanthridines - chemistry ; Benzophenanthridines - isolation & purification ; Benzophenanthridines - pharmacology ; Cell Line ; Cell Line, Tumor ; Cercopithecus aethiops ; Colorectal Neoplasms ; Humans ; Kidney - drug effects ; Kinetics ; Madagascar ; Vero Cells ; Zanthoxylum</subject><ispartof>In vivo (Athens), 2007-03, Vol.21 (2), p.417-422</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17436597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pachon, G</creatorcontrib><creatorcontrib>Rasoanaivo, H</creatorcontrib><creatorcontrib>Azqueta, A</creatorcontrib><creatorcontrib>Rakotozafy, J C</creatorcontrib><creatorcontrib>Raharisololalao, A</creatorcontrib><creatorcontrib>De Cerain, A López</creatorcontrib><creatorcontrib>De Lapuente, J</creatorcontrib><creatorcontrib>Borràs, M</creatorcontrib><creatorcontrib>Moukha, S</creatorcontrib><creatorcontrib>Centelles, J J</creatorcontrib><creatorcontrib>Creppy, E E</creatorcontrib><creatorcontrib>Cascante, M</creatorcontrib><title>Anticancer Effect of a New Benzophenanthridine Isolated from Zanthoxylum madagascariense (Rutaceline)</title><title>In vivo (Athens)</title><addtitle>In Vivo</addtitle><description>Fractionation of the cyclohexane extract from the stem bark powder of Zanthoxylum madagascariense led to the isolation of
a new benzophenanthridine-type alkaloid, hydrochloride of 2,3-methylendioxy-8-hydroxy-7-methoxy-benzo[C]phenanthridine (Rutaceline),
characterized on the basis of its spectral data. Rutaceline was evaluated for its antiproliferative capacity on the human
colorectal adenocarcinoma (Caco-2) and the African green monkey kidney (Vero) cell lines. The 50% inhibition of cell growth
(IC 50 ) obtained after 24 h incubation was similar for both cells lines (110-115 μg/ml, i.e. 269-281 μM), but at 48 h the IC 50 value for the Caco-2 cells was lower than for the Vero cells (20 μg/ml, i.e. 49 μM versus 90 μg/ml, i.e. 220 μM) indicating
a higher cell growth inhibitory effect on the colon adenocarcinoma cells. At the respective IC 50 concentrations, Rutaceline did not significantly induce apoptosis but induced cell cycle arrest in the G0/G1 phase, as well
as a decrease of cells in S phase. Rutaceline also induced DNA fragmentation in both cell lines, as revealed by agarose gel
electrophoresis, and a dose-dependent clastogenic effect in both cell lines as revealed by the Comet assay.</description><subject>Adenocarcinoma</subject><subject>Animals</subject><subject>Antineoplastic Agents - chemistry</subject><subject>Antineoplastic Agents - isolation & purification</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Benzophenanthridines - chemistry</subject><subject>Benzophenanthridines - isolation & purification</subject><subject>Benzophenanthridines - pharmacology</subject><subject>Cell Line</subject><subject>Cell Line, Tumor</subject><subject>Cercopithecus aethiops</subject><subject>Colorectal Neoplasms</subject><subject>Humans</subject><subject>Kidney - drug effects</subject><subject>Kinetics</subject><subject>Madagascar</subject><subject>Vero Cells</subject><subject>Zanthoxylum</subject><issn>0258-851X</issn><issn>1791-7549</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo10E1LxDAQBuAgirt-_AXJQUQPhbRpmua4yqoLi4IoiJeQJpNtpE3XpHVdf72V1dMc5nlfhtlD05SLNOEsF_toSjJWJiVLXyfoKMZ3QgpOSHaIJinPacEEnyKY-d5p5TUEPLcWdI87ixV-gA2-Bv_drWvwyvd1cMZ5wIvYNaoHg23oWvz2u-m-ts3Q4lYZtVJRq-DAR8CXT0OvNDRj6uoEHVjVRDj9m8fo5Xb-fHOfLB_vFjezZVJntOwTboAUhhBNVUnLqtKqosZaltms4GALqnOdCmbysZflBTFClJDZSjBRAVU5PUYXu9516D4GiL1sXRxvaJSHboiSE1oykpMRnv3BoWrByHVwrQpb-f-YEZzvQO1W9cYFkLFVTTNyKt1nlspM5imnP5BObtQ</recordid><startdate>20070301</startdate><enddate>20070301</enddate><creator>Pachon, G</creator><creator>Rasoanaivo, H</creator><creator>Azqueta, A</creator><creator>Rakotozafy, J C</creator><creator>Raharisololalao, A</creator><creator>De Cerain, A López</creator><creator>De Lapuente, J</creator><creator>Borràs, M</creator><creator>Moukha, S</creator><creator>Centelles, J J</creator><creator>Creppy, E E</creator><creator>Cascante, M</creator><general>International Institute of Anticancer Research</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20070301</creationdate><title>Anticancer Effect of a New Benzophenanthridine Isolated from Zanthoxylum madagascariense (Rutaceline)</title><author>Pachon, G ; Rasoanaivo, H ; Azqueta, A ; Rakotozafy, J C ; Raharisololalao, A ; De Cerain, A López ; De Lapuente, J ; Borràs, M ; Moukha, S ; Centelles, J J ; Creppy, E E ; Cascante, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h238t-7de06d00c3a838bbcab3dff52f267ef63c4c195d4ace5460d998e2fb959be3a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adenocarcinoma</topic><topic>Animals</topic><topic>Antineoplastic Agents - chemistry</topic><topic>Antineoplastic Agents - isolation & purification</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Benzophenanthridines - chemistry</topic><topic>Benzophenanthridines - isolation & purification</topic><topic>Benzophenanthridines - pharmacology</topic><topic>Cell Line</topic><topic>Cell Line, Tumor</topic><topic>Cercopithecus aethiops</topic><topic>Colorectal Neoplasms</topic><topic>Humans</topic><topic>Kidney - drug effects</topic><topic>Kinetics</topic><topic>Madagascar</topic><topic>Vero Cells</topic><topic>Zanthoxylum</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pachon, G</creatorcontrib><creatorcontrib>Rasoanaivo, H</creatorcontrib><creatorcontrib>Azqueta, A</creatorcontrib><creatorcontrib>Rakotozafy, J C</creatorcontrib><creatorcontrib>Raharisololalao, A</creatorcontrib><creatorcontrib>De Cerain, A López</creatorcontrib><creatorcontrib>De Lapuente, J</creatorcontrib><creatorcontrib>Borràs, M</creatorcontrib><creatorcontrib>Moukha, S</creatorcontrib><creatorcontrib>Centelles, J J</creatorcontrib><creatorcontrib>Creppy, E E</creatorcontrib><creatorcontrib>Cascante, M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>In vivo (Athens)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pachon, G</au><au>Rasoanaivo, H</au><au>Azqueta, A</au><au>Rakotozafy, J C</au><au>Raharisololalao, A</au><au>De Cerain, A López</au><au>De Lapuente, J</au><au>Borràs, M</au><au>Moukha, S</au><au>Centelles, J J</au><au>Creppy, E E</au><au>Cascante, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anticancer Effect of a New Benzophenanthridine Isolated from Zanthoxylum madagascariense (Rutaceline)</atitle><jtitle>In vivo (Athens)</jtitle><addtitle>In Vivo</addtitle><date>2007-03-01</date><risdate>2007</risdate><volume>21</volume><issue>2</issue><spage>417</spage><epage>422</epage><pages>417-422</pages><issn>0258-851X</issn><eissn>1791-7549</eissn><abstract>Fractionation of the cyclohexane extract from the stem bark powder of Zanthoxylum madagascariense led to the isolation of
a new benzophenanthridine-type alkaloid, hydrochloride of 2,3-methylendioxy-8-hydroxy-7-methoxy-benzo[C]phenanthridine (Rutaceline),
characterized on the basis of its spectral data. Rutaceline was evaluated for its antiproliferative capacity on the human
colorectal adenocarcinoma (Caco-2) and the African green monkey kidney (Vero) cell lines. The 50% inhibition of cell growth
(IC 50 ) obtained after 24 h incubation was similar for both cells lines (110-115 μg/ml, i.e. 269-281 μM), but at 48 h the IC 50 value for the Caco-2 cells was lower than for the Vero cells (20 μg/ml, i.e. 49 μM versus 90 μg/ml, i.e. 220 μM) indicating
a higher cell growth inhibitory effect on the colon adenocarcinoma cells. At the respective IC 50 concentrations, Rutaceline did not significantly induce apoptosis but induced cell cycle arrest in the G0/G1 phase, as well
as a decrease of cells in S phase. Rutaceline also induced DNA fragmentation in both cell lines, as revealed by agarose gel
electrophoresis, and a dose-dependent clastogenic effect in both cell lines as revealed by the Comet assay.</abstract><cop>Greece</cop><pub>International Institute of Anticancer Research</pub><pmid>17436597</pmid><tpages>6</tpages></addata></record> |
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| subjects | Adenocarcinoma Animals Antineoplastic Agents - chemistry Antineoplastic Agents - isolation & purification Antineoplastic Agents - pharmacology Benzophenanthridines - chemistry Benzophenanthridines - isolation & purification Benzophenanthridines - pharmacology Cell Line Cell Line, Tumor Cercopithecus aethiops Colorectal Neoplasms Humans Kidney - drug effects Kinetics Madagascar Vero Cells Zanthoxylum |
| title | Anticancer Effect of a New Benzophenanthridine Isolated from Zanthoxylum madagascariense (Rutaceline) |
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