Preparation of thermoresponsive polymer brush surfaces and their interaction with cells
Abstract Poly( N -isopropylacrylamide) (PIPAAm) brush surfaces with different layer thickness on polystyrene substrates were prepared by surface-initiated atom transfer radical polymerization (ATRP). Surface characteristics of PIPAAm brushes and their influence on adhesion and detachment of bovine c...
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| Veröffentlicht in: | Biomaterials 2008-05, Vol.29 (13), p.2073-2081 |
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| creator | Mizutani, Aya Kikuchi, Akihiko Yamato, Masayuki Kanazawa, Hideko Okano, Teruo |
| description | Abstract Poly( N -isopropylacrylamide) (PIPAAm) brush surfaces with different layer thickness on polystyrene substrates were prepared by surface-initiated atom transfer radical polymerization (ATRP). Surface characteristics of PIPAAm brushes and their influence on adhesion and detachment of bovine carotid artery endothelial cells (ECs) were controlled by PIPAAm layer thickness. Surface hydrophilicity increased with PIPAAm layer thickness at 37 °C because PIPAAm brush surfaces with higher thickness provide more extended chain conformations with relatively high chain mobility, and accompanying polymer chain hydration. These surface property alterations lead to negligible cell adhesion through minimal matrix protein adsorption and also modified surface modulus. By adjusting polymerization reaction conditions and time, polymer layers supporting confluent cultures of ECs were possible. Confluent EC monolayers spontaneously detached as contiguous cell sheets from PIPAAm brush surfaces at reduced temperatures. Thermoresponsive cell adhesion and detachment behavior were analyzed from the standpoint of surface physicochemical characteristics. Thermoresponsive surfaces prepared by surface-initiated ATRP techniques allow surface selection in preparing cell sheets from attachment-dependent cells having relatively strong adhesive property for tissue engineering applications. |
| doi_str_mv | 10.1016/j.biomaterials.2008.01.004 |
| format | Article |
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Surface characteristics of PIPAAm brushes and their influence on adhesion and detachment of bovine carotid artery endothelial cells (ECs) were controlled by PIPAAm layer thickness. Surface hydrophilicity increased with PIPAAm layer thickness at 37 °C because PIPAAm brush surfaces with higher thickness provide more extended chain conformations with relatively high chain mobility, and accompanying polymer chain hydration. These surface property alterations lead to negligible cell adhesion through minimal matrix protein adsorption and also modified surface modulus. By adjusting polymerization reaction conditions and time, polymer layers supporting confluent cultures of ECs were possible. Confluent EC monolayers spontaneously detached as contiguous cell sheets from PIPAAm brush surfaces at reduced temperatures. Thermoresponsive cell adhesion and detachment behavior were analyzed from the standpoint of surface physicochemical characteristics. Thermoresponsive surfaces prepared by surface-initiated ATRP techniques allow surface selection in preparing cell sheets from attachment-dependent cells having relatively strong adhesive property for tissue engineering applications.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2008.01.004</identifier><identifier>PMID: 18261791</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Acrylic Resins - chemistry ; Advanced Basic Science ; Animals ; Atom transfer radical polymerization ; Cattle ; Cell Adhesion ; Cell culture ; Cell Shape ; Cells, Cultured ; Dentistry ; Endothelial Cells - chemistry ; Endothelial Cells - cytology ; Endothelial Cells - metabolism ; Fibronectins - metabolism ; Free Radicals - chemistry ; Molecular Structure ; Poly( N-isopropylacrylamide) ; Polystyrene substrates ; Surface Properties ; Temperature ; Thermoresponsive polymer</subject><ispartof>Biomaterials, 2008-05, Vol.29 (13), p.2073-2081</ispartof><rights>Elsevier Ltd</rights><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c495t-8798dbcd293327f08f3342606ead4db528ce709ac81751465b7937fd5b6b36583</citedby><cites>FETCH-LOGICAL-c495t-8798dbcd293327f08f3342606ead4db528ce709ac81751465b7937fd5b6b36583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2008.01.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18261791$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mizutani, Aya</creatorcontrib><creatorcontrib>Kikuchi, Akihiko</creatorcontrib><creatorcontrib>Yamato, Masayuki</creatorcontrib><creatorcontrib>Kanazawa, Hideko</creatorcontrib><creatorcontrib>Okano, Teruo</creatorcontrib><title>Preparation of thermoresponsive polymer brush surfaces and their interaction with cells</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Poly( N -isopropylacrylamide) (PIPAAm) brush surfaces with different layer thickness on polystyrene substrates were prepared by surface-initiated atom transfer radical polymerization (ATRP). Surface characteristics of PIPAAm brushes and their influence on adhesion and detachment of bovine carotid artery endothelial cells (ECs) were controlled by PIPAAm layer thickness. Surface hydrophilicity increased with PIPAAm layer thickness at 37 °C because PIPAAm brush surfaces with higher thickness provide more extended chain conformations with relatively high chain mobility, and accompanying polymer chain hydration. These surface property alterations lead to negligible cell adhesion through minimal matrix protein adsorption and also modified surface modulus. By adjusting polymerization reaction conditions and time, polymer layers supporting confluent cultures of ECs were possible. Confluent EC monolayers spontaneously detached as contiguous cell sheets from PIPAAm brush surfaces at reduced temperatures. Thermoresponsive cell adhesion and detachment behavior were analyzed from the standpoint of surface physicochemical characteristics. Thermoresponsive surfaces prepared by surface-initiated ATRP techniques allow surface selection in preparing cell sheets from attachment-dependent cells having relatively strong adhesive property for tissue engineering applications.</description><subject>Acrylic Resins - chemistry</subject><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Atom transfer radical polymerization</subject><subject>Cattle</subject><subject>Cell Adhesion</subject><subject>Cell culture</subject><subject>Cell Shape</subject><subject>Cells, Cultured</subject><subject>Dentistry</subject><subject>Endothelial Cells - chemistry</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - metabolism</subject><subject>Fibronectins - metabolism</subject><subject>Free Radicals - chemistry</subject><subject>Molecular Structure</subject><subject>Poly( N-isopropylacrylamide)</subject><subject>Polystyrene substrates</subject><subject>Surface Properties</subject><subject>Temperature</subject><subject>Thermoresponsive polymer</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks2L1TAUxYMoznP0X5Diwl3rTdJ8uRBkxi8YUFBxGdL0lpdn29SkHXn_va3vgeJmXIXA75x7uOcS8oxCRYHKF4eqCXFwM6bg-lwxAF0BrQDqe2RHtdKlMCDukx3QmpVGUnZBHuV8gPUPNXtILqhmkipDd-Tbp4STS24OcSxiV8x7TENMmKc45nCLxRT744CpaNKS90VeUuc85sKN7caGVIRxDeL8b4OfYd4XHvs-PyYPujUcPjm_l-Tr2zdfrt6XNx_ffbh6fVP62oi51MrotvEtM5wz1YHuOK-ZBImurdtGMO1RgXFeUyVoLUWjDFddKxrZcCk0vyTPT75Tij8WzLMdQt4SuBHjkq0CrqEGcyfImTCK8btBBkJKKfgKvjyBPsWcE3Z2SmFw6Wgp2K0oe7B_F2W3oixQuxa1ip-epyzNgO0f6bmZFbg-Abhu7zZgstkHHD22IaGfbRvD_8159Y-N78MYvOu_4xHzIS5p3DTUZmbBft5OZrsY0ADAmOK_ANdAwLY</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Mizutani, Aya</creator><creator>Kikuchi, Akihiko</creator><creator>Yamato, Masayuki</creator><creator>Kanazawa, Hideko</creator><creator>Okano, Teruo</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20080501</creationdate><title>Preparation of thermoresponsive polymer brush surfaces and their interaction with cells</title><author>Mizutani, Aya ; Kikuchi, Akihiko ; Yamato, Masayuki ; Kanazawa, Hideko ; Okano, Teruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c495t-8798dbcd293327f08f3342606ead4db528ce709ac81751465b7937fd5b6b36583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acrylic Resins - chemistry</topic><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Atom transfer radical polymerization</topic><topic>Cattle</topic><topic>Cell Adhesion</topic><topic>Cell culture</topic><topic>Cell Shape</topic><topic>Cells, Cultured</topic><topic>Dentistry</topic><topic>Endothelial Cells - chemistry</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - metabolism</topic><topic>Fibronectins - metabolism</topic><topic>Free Radicals - chemistry</topic><topic>Molecular Structure</topic><topic>Poly( N-isopropylacrylamide)</topic><topic>Polystyrene substrates</topic><topic>Surface Properties</topic><topic>Temperature</topic><topic>Thermoresponsive polymer</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mizutani, Aya</creatorcontrib><creatorcontrib>Kikuchi, Akihiko</creatorcontrib><creatorcontrib>Yamato, Masayuki</creatorcontrib><creatorcontrib>Kanazawa, Hideko</creatorcontrib><creatorcontrib>Okano, Teruo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mizutani, Aya</au><au>Kikuchi, Akihiko</au><au>Yamato, Masayuki</au><au>Kanazawa, Hideko</au><au>Okano, Teruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of thermoresponsive polymer brush surfaces and their interaction with cells</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>29</volume><issue>13</issue><spage>2073</spage><epage>2081</epage><pages>2073-2081</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Poly( N -isopropylacrylamide) (PIPAAm) brush surfaces with different layer thickness on polystyrene substrates were prepared by surface-initiated atom transfer radical polymerization (ATRP). Surface characteristics of PIPAAm brushes and their influence on adhesion and detachment of bovine carotid artery endothelial cells (ECs) were controlled by PIPAAm layer thickness. Surface hydrophilicity increased with PIPAAm layer thickness at 37 °C because PIPAAm brush surfaces with higher thickness provide more extended chain conformations with relatively high chain mobility, and accompanying polymer chain hydration. These surface property alterations lead to negligible cell adhesion through minimal matrix protein adsorption and also modified surface modulus. By adjusting polymerization reaction conditions and time, polymer layers supporting confluent cultures of ECs were possible. Confluent EC monolayers spontaneously detached as contiguous cell sheets from PIPAAm brush surfaces at reduced temperatures. Thermoresponsive cell adhesion and detachment behavior were analyzed from the standpoint of surface physicochemical characteristics. Thermoresponsive surfaces prepared by surface-initiated ATRP techniques allow surface selection in preparing cell sheets from attachment-dependent cells having relatively strong adhesive property for tissue engineering applications.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>18261791</pmid><doi>10.1016/j.biomaterials.2008.01.004</doi><tpages>9</tpages></addata></record> |
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| subjects | Acrylic Resins - chemistry Advanced Basic Science Animals Atom transfer radical polymerization Cattle Cell Adhesion Cell culture Cell Shape Cells, Cultured Dentistry Endothelial Cells - chemistry Endothelial Cells - cytology Endothelial Cells - metabolism Fibronectins - metabolism Free Radicals - chemistry Molecular Structure Poly( N-isopropylacrylamide) Polystyrene substrates Surface Properties Temperature Thermoresponsive polymer |
| title | Preparation of thermoresponsive polymer brush surfaces and their interaction with cells |
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