Real-time multiplex PCR assay for rapid detection and toxintyping of Clostridium perfringens toxin producing strains in feces of dairy cattle

Clostridium perfringens is an anaerobic, gram-positive, spore-forming bacterium associated with a wide variety of diseases in domestic animals and humans. We have developed dual-labeled fluorescence hybridization probe (TaqMan ®)-based real-time multiplex PCR assay for detection of toxin genes alpha...

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Veröffentlicht in:	Molecular and cellular probes 2008-04, Vol.22 (2), p.90-95
Hauptverfasser:	Gurjar, A.A., Hegde, N.V., Love, B.C., Jayarao, B.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Toxins - metabolism Bacterial Typing Techniques - methods Beta2 toxin Cattle Clostridium perfringens Clostridium perfringens - classification Clostridium perfringens - genetics Clostridium perfringens - metabolism Dual-labeled fluorescence hybridization probe Feces - microbiology Pennsylvania Polymerase Chain Reaction - methods Real-time multiplex PCR Swine Diseases - microbiology
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