A Microfluidic System for Saliva-Based Detection of Infectious Diseases
: A “lab‐on‐a‐chip” system for detecting bacterial pathogens in oral fluid samples is described. The system comprises: (1) an oral fluid sample collector; (2) a disposable, plastic microfluidic cassette (“chip”) for sample processing including immunochromatographic assay with a nitrocellulose later...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 2007-03, Vol.1098 (1), p.429-436 |
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| container_title | Annals of the New York Academy of Sciences |
| container_volume | 1098 |
| creator | CHEN, ZONGYUAN MAUK, MICHAEL G. WANG, JING ABRAMS, WILLIAM R. CORSTJENS, PAUL L. A. M. NIEDBALA, R. SAM MALAMUD, DANIEL BAU, HAIM H. |
| description | : A “lab‐on‐a‐chip” system for detecting bacterial pathogens in oral fluid samples is described. The system comprises: (1) an oral fluid sample collector; (2) a disposable, plastic microfluidic cassette (“chip”) for sample processing including immunochromatographic assay with a nitrocellulose lateral flow strip; (3) a platform that controls the cassette operation by providing metered quantities of reagents, temperature regulation, valve actuation; and (4) a laser scanner to interrogate the lateral flow strip. The microfluidic chip hosts a fluidic network for cell lysis, nucleic acid extraction and isolation, PCR, and labeling of the PCR product with bioconjugated, upconverting phosphor particles for detection on the lateral flow strip. |
| doi_str_mv | 10.1196/annals.1384.024 |
| format | Article |
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The microfluidic chip hosts a fluidic network for cell lysis, nucleic acid extraction and isolation, PCR, and labeling of the PCR product with bioconjugated, upconverting phosphor particles for detection on the lateral flow strip.</description><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial Infections - diagnosis</subject><subject>Bacterial Infections - microbiology</subject><subject>Cells, Cultured</subject><subject>diagnostics</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>Humans</subject><subject>immunochromatography</subject><subject>infectious diseases</subject><subject>lab-on-a-chip</subject><subject>Lab-On-A-Chip Devices</subject><subject>microfluidics</subject><subject>Microfluidics - instrumentation</subject><subject>Polymerase Chain Reaction</subject><subject>Saliva - microbiology</subject><issn>0077-8923</issn><issn>1749-6632</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PAjEQhhujEUTP3syevC30i53tEUWBiChBYzw1pdsm1WUXt6Dy7y0s0aOnySTP-2bmQeic4DYhIumoolC5bxOW8jam_AA1CXARJwmjh6iJMUCcCsoa6MT7N4wJTTkco0aAWJdwaKJBL7p3uiptvnaZ09Fs41dmEdmyimYqd58qvlLeZFHfrIxeubKIShuNCrtb1j7qO28C4E_RkQ2XmLP9bKHn25un62E8fhiMrnvjWDMAHmegCGMYz7lmWTrnmJsEulxrARnFmFmmu1QpoAaoNSLBGjjRWNlUCMoFZi10Wfcuq_JjbfxKLpzXJs9VYcI9EjADwSANYKcGw3PeV8bKZeUWqtpIguXWnazdya07GdyFxMW-ej1fmOyP38sKAKuBL5ebzX99cvLam-1q4zrlgtnv35Sq3mUCDLryZTKQj5De8enwSk7ZD8cJic8</recordid><startdate>200703</startdate><enddate>200703</enddate><creator>CHEN, ZONGYUAN</creator><creator>MAUK, MICHAEL G.</creator><creator>WANG, JING</creator><creator>ABRAMS, WILLIAM R.</creator><creator>CORSTJENS, PAUL L. 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| ispartof | Annals of the New York Academy of Sciences, 2007-03, Vol.1098 (1), p.429-436 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Bacteria - genetics Bacteria - isolation & purification Bacterial Infections - diagnosis Bacterial Infections - microbiology Cells, Cultured diagnostics DNA, Bacterial - analysis DNA, Bacterial - genetics Humans immunochromatography infectious diseases lab-on-a-chip Lab-On-A-Chip Devices microfluidics Microfluidics - instrumentation Polymerase Chain Reaction Saliva - microbiology |
| title | A Microfluidic System for Saliva-Based Detection of Infectious Diseases |
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