PTH regulation of the human cytomegalovirus immediate-early gene promoter
Secondary hyperparathyroidism and human cytomegalovirus (hCMV) seropositivity are highly prevalent in patients undergoing renal transplantation, and both are linked to the development of chronic allograft nephropathy (CAN). We investigated the hypothesis that parathyroid hormone (PTH) 1–84 regulates...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2008-04, Vol.368 (4), p.977-982 |
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| creator | Herberth, Johann Alimov, Alexander P. Williams, John P. Malluche, Hartmut H. Koszewski, Nicholas J. |
| description | Secondary hyperparathyroidism and human cytomegalovirus (hCMV) seropositivity are highly prevalent in patients undergoing renal transplantation, and both are linked to the development of chronic allograft nephropathy (CAN). We investigated the hypothesis that parathyroid hormone (PTH) 1–84 regulates hCMV immediate-early gene (IEG) promoter activation in proximal renal tubular cells. PTH 1–84 enhanced hCMV IEG promoter (−548 to +92) activity in opossum kidney cells. Deletion analysis from the 5′ end of the promoter localized the PTH 1–84 associated activity to the DNA sequence between −123 and −45. Mutation of an imperfect ATF/AP-1 DNA element within this region abrogated the PTH 1–84 effect and also strongly attenuated basal gene expression. Mobility shift analyses using this DNA element revealed that a member of the ATF-1 family was in the binding complex. In summary, we present evidence for a novel pathogenic role of PTH 1–84 in promoting hCMV immediate-early gene transcription. |
| doi_str_mv | 10.1016/j.bbrc.2008.02.019 |
| format | Article |
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We investigated the hypothesis that parathyroid hormone (PTH) 1–84 regulates hCMV immediate-early gene (IEG) promoter activation in proximal renal tubular cells. PTH 1–84 enhanced hCMV IEG promoter (−548 to +92) activity in opossum kidney cells. Deletion analysis from the 5′ end of the promoter localized the PTH 1–84 associated activity to the DNA sequence between −123 and −45. Mutation of an imperfect ATF/AP-1 DNA element within this region abrogated the PTH 1–84 effect and also strongly attenuated basal gene expression. Mobility shift analyses using this DNA element revealed that a member of the ATF-1 family was in the binding complex. 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We investigated the hypothesis that parathyroid hormone (PTH) 1–84 regulates hCMV immediate-early gene (IEG) promoter activation in proximal renal tubular cells. PTH 1–84 enhanced hCMV IEG promoter (−548 to +92) activity in opossum kidney cells. Deletion analysis from the 5′ end of the promoter localized the PTH 1–84 associated activity to the DNA sequence between −123 and −45. Mutation of an imperfect ATF/AP-1 DNA element within this region abrogated the PTH 1–84 effect and also strongly attenuated basal gene expression. Mobility shift analyses using this DNA element revealed that a member of the ATF-1 family was in the binding complex. In summary, we present evidence for a novel pathogenic role of PTH 1–84 in promoting hCMV immediate-early gene transcription.</description><subject>Activating Transcription Factor 1 - physiology</subject><subject>Animals</subject><subject>ATF-1</subject><subject>Cell Line</subject><subject>Chronic allograft nephropathy</subject><subject>CREB</subject><subject>Cytomegalovirus - genetics</subject><subject>DNA</subject><subject>Genes, Immediate-Early - genetics</subject><subject>Human cytomegalovirus</subject><subject>Humans</subject><subject>Immediate-early gene</subject><subject>Kidney</subject><subject>Opossums</subject><subject>Parathyroid hormone</subject><subject>Parathyroid Hormone - pharmacology</subject><subject>Promoter Regions, Genetic - drug effects</subject><subject>Transcription</subject><subject>Transfection</subject><subject>Tubular cells</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E9L5DAYx_EgKzr-eQN7WHraW-vzNJ00gb2IuCoIelDwFpL06ZihbdykHZh3vx1mwJuecvk-v8CHsZ8IBQKKq3VhbXRFCSALKAtAdcQWCAryEqH6wRYAIPJS4dspO0tpDYBYCXXCTlGWtRJCLdjD88t9Fmk1dWb0YchCm43vlL1PvRkytx1DTyvThY2PU8p831PjzUg5mdhtsxUNlH3E0IeR4gU7bk2X6PLwnrPXv7cvN_f549Pdw831Y-44F2NOioSSSwUOjVi2ZCXyGrmkusGmoRZrshwqWLYWaoGmkRaUc5WwCpU1kp-z3_vd-eN_E6VR9z456jozUJiSroHXsubVtyEqicBLNYflPnQxpBSp1R_R9yZuNYLeSeu13knrnbSGUs_S89Gvw_pkZ5XPkwPtHPzZBzRjbDxFnZynwc2Ckdyom-C_2v8PaUOPpQ</recordid><startdate>20080418</startdate><enddate>20080418</enddate><creator>Herberth, Johann</creator><creator>Alimov, Alexander P.</creator><creator>Williams, John P.</creator><creator>Malluche, Hartmut H.</creator><creator>Koszewski, Nicholas J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080418</creationdate><title>PTH regulation of the human cytomegalovirus immediate-early gene promoter</title><author>Herberth, Johann ; Alimov, Alexander P. ; Williams, John P. ; Malluche, Hartmut H. ; Koszewski, Nicholas J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c336t-e9e698590c1a65feb8137138e7d1ddef17eb30405fb0761ad8b09cc46b919ba83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Activating Transcription Factor 1 - physiology</topic><topic>Animals</topic><topic>ATF-1</topic><topic>Cell Line</topic><topic>Chronic allograft nephropathy</topic><topic>CREB</topic><topic>Cytomegalovirus - genetics</topic><topic>DNA</topic><topic>Genes, Immediate-Early - genetics</topic><topic>Human cytomegalovirus</topic><topic>Humans</topic><topic>Immediate-early gene</topic><topic>Kidney</topic><topic>Opossums</topic><topic>Parathyroid hormone</topic><topic>Parathyroid Hormone - pharmacology</topic><topic>Promoter Regions, Genetic - drug effects</topic><topic>Transcription</topic><topic>Transfection</topic><topic>Tubular cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Herberth, Johann</creatorcontrib><creatorcontrib>Alimov, Alexander P.</creatorcontrib><creatorcontrib>Williams, John P.</creatorcontrib><creatorcontrib>Malluche, Hartmut H.</creatorcontrib><creatorcontrib>Koszewski, Nicholas J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Herberth, Johann</au><au>Alimov, Alexander P.</au><au>Williams, John P.</au><au>Malluche, Hartmut H.</au><au>Koszewski, Nicholas J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PTH regulation of the human cytomegalovirus immediate-early gene promoter</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2008-04-18</date><risdate>2008</risdate><volume>368</volume><issue>4</issue><spage>977</spage><epage>982</epage><pages>977-982</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Secondary hyperparathyroidism and human cytomegalovirus (hCMV) seropositivity are highly prevalent in patients undergoing renal transplantation, and both are linked to the development of chronic allograft nephropathy (CAN). 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| subjects | Activating Transcription Factor 1 - physiology Animals ATF-1 Cell Line Chronic allograft nephropathy CREB Cytomegalovirus - genetics DNA Genes, Immediate-Early - genetics Human cytomegalovirus Humans Immediate-early gene Kidney Opossums Parathyroid hormone Parathyroid Hormone - pharmacology Promoter Regions, Genetic - drug effects Transcription Transfection Tubular cells |
| title | PTH regulation of the human cytomegalovirus immediate-early gene promoter |
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