Direct multiplexed measurement of gene expression with color-coded probe pairs
We describe a technology, the NanoString nCounter gene expression system, which captures and counts individual mRNA transcripts. Advantages over existing platforms include direct measurement of mRNA expression levels without enzymatic reactions or bias, sensitivity coupled with high multiplex capabi...
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| Veröffentlicht in: | Nature biotechnology 2008-03, Vol.26 (3), p.317-325 |
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| creator | Geiss, Gary K Bumgarner, Roger E Birditt, Brian Dahl, Timothy Dowidar, Naeem Dunaway, Dwayne L Fell, H Perry Ferree, Sean George, Renee D Grogan, Tammy James, Jeffrey J Maysuria, Malini Mitton, Jeffrey D Oliveri, Paola Osborn, Jennifer L Peng, Tao Ratcliffe, Amber L Webster, Philippa J Davidson, Eric H Hood, Leroy Dimitrov, Krassen |
| description | We describe a technology, the NanoString nCounter gene expression system, which captures and counts individual mRNA transcripts. Advantages over existing platforms include direct measurement of mRNA expression levels without enzymatic reactions or bias, sensitivity coupled with high multiplex capability, and digital readout. Experiments performed on 509 human genes yielded a replicate correlation coefficient of 0.999, a detection limit between 0.1 fM and 0.5 fM, and a linear dynamic range of over 500-fold. Comparison of the NanoString nCounter gene expression system with microarrays and TaqMan PCR demonstrated that the nCounter system is more sensitive than microarrays and similar in sensitivity to real-time PCR. Finally, a comparison of transcript levels for 21 genes across seven samples measured by the nCounter system and SYBR Green real-time PCR demonstrated similar patterns of gene expression at all transcript levels. |
| doi_str_mv | 10.1038/nbt1385 |
| format | Article |
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Advantages over existing platforms include direct measurement of mRNA expression levels without enzymatic reactions or bias, sensitivity coupled with high multiplex capability, and digital readout. Experiments performed on 509 human genes yielded a replicate correlation coefficient of 0.999, a detection limit between 0.1 fM and 0.5 fM, and a linear dynamic range of over 500-fold. Comparison of the NanoString nCounter gene expression system with microarrays and TaqMan PCR demonstrated that the nCounter system is more sensitive than microarrays and similar in sensitivity to real-time PCR. 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| ispartof | Nature biotechnology, 2008-03, Vol.26 (3), p.317-325 |
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| source | MEDLINE; Nature; Alma/SFX Local Collection |
| subjects | Agriculture Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Cell Line Color Correlation coefficient Diverse techniques DNA microarrays DNA Probes - genetics DNA Probes - metabolism DNA testing Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Profiling - methods Gene Library Genes, Reporter Humans Image Processing, Computer-Assisted Innovations Life Sciences Measurement Methods Molecular and cellular biology Nanotechnology - instrumentation Nanotechnology - methods Oligonucleotide Array Sequence Analysis Poliovirus Polymerase Chain Reaction Reproducibility of Results Research methodology Ribonucleic acid RNA RNA, Messenger - analysis RNA, Messenger - genetics Sensitivity and Specificity |
| title | Direct multiplexed measurement of gene expression with color-coded probe pairs |
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