Validated method for the quantification of artepillin-C in Brazilian propolis
Brazilian propolis contains several phenolic compounds among which 5 diprenyl-4-hydroxycinnamic acid (artepillin-C) is commonly found in areas where flora is rich in Baccharis species. The quantification of artepillin-C has become an important factor as an indicator of Brazilian propolis quality and...
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| Veröffentlicht in: | Phytochemical analysis 2008-03, Vol.19 (2), p.179-183 |
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| creator | Matsuda, Adriana Hitomi de Almeida-Muradian, Ligia Bicudo |
| description | Brazilian propolis contains several phenolic compounds among which 5 diprenyl-4-hydroxycinnamic acid (artepillin-C) is commonly found in areas where flora is rich in Baccharis species. The quantification of artepillin-C has become an important factor as an indicator of Brazilian propolis quality and the compound may be used as a chemical marker for quality control in exportating green propolis. This work was to validate the method and evaluate the content of artepillin-C from 33 samples collected in different Brazilian regions. The method used was HPLC with UV-vis detection and a reversed-phase C₁₈ column. The validation parameters studied were: linearity, accuracy, precision, quantification and detection limits. The results obtained were: detection limit = 0.0036 μg/mL, quantification limit = 0.012 μg/mL, accuracy = 0.0064 and 0.078, recovery 98-102%. Artepillin-C content varied from 0 to 11% depending on the geographical origin. Propolis from the southeast region presented the highest level of artepillin-C (5.0-11.0%). Whist that from the northeast region did not show any artepillin-C. Copyright © 2008 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/pca.1043 |
| format | Article |
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The quantification of artepillin-C has become an important factor as an indicator of Brazilian propolis quality and the compound may be used as a chemical marker for quality control in exportating green propolis. This work was to validate the method and evaluate the content of artepillin-C from 33 samples collected in different Brazilian regions. The method used was HPLC with UV-vis detection and a reversed-phase C₁₈ column. The validation parameters studied were: linearity, accuracy, precision, quantification and detection limits. The results obtained were: detection limit = 0.0036 μg/mL, quantification limit = 0.012 μg/mL, accuracy = 0.0064 and 0.078, recovery 98-102%. Artepillin-C content varied from 0 to 11% depending on the geographical origin. Propolis from the southeast region presented the highest level of artepillin-C (5.0-11.0%). Whist that from the northeast region did not show any artepillin-C. Copyright © 2008 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0958-0344</identifier><identifier>EISSN: 1099-1565</identifier><identifier>DOI: 10.1002/pca.1043</identifier><identifier>PMID: 18200605</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>5-diprenyl-4-hydroxycinnamic acid ; artepellin-C ; artepillin ; Brazil ; chemical analysis ; chromatography ; Chromatography, High Pressure Liquid - methods ; Chromatography, High Pressure Liquid - standards ; high performance liquid chromatography ; HPLC ; phenolic compounds ; Phenylpropionates - analysis ; Phenylpropionates - chemistry ; propolis ; Propolis - chemistry ; Quality Control ; quantitative analysis ; Reproducibility of Results ; validation</subject><ispartof>Phytochemical analysis, 2008-03, Vol.19 (2), p.179-183</ispartof><rights>Copyright © 2007 John Wiley & Sons, Ltd.</rights><rights>Copyright 2008 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4473-8961f528742d495368e262920032a4eedb51756f7058c7a38dc76242d0ce8bdc3</citedby><cites>FETCH-LOGICAL-c4473-8961f528742d495368e262920032a4eedb51756f7058c7a38dc76242d0ce8bdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpca.1043$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpca.1043$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18200605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuda, Adriana Hitomi</creatorcontrib><creatorcontrib>de Almeida-Muradian, Ligia Bicudo</creatorcontrib><title>Validated method for the quantification of artepillin-C in Brazilian propolis</title><title>Phytochemical analysis</title><addtitle>Phytochem. Anal</addtitle><description>Brazilian propolis contains several phenolic compounds among which 5 diprenyl-4-hydroxycinnamic acid (artepillin-C) is commonly found in areas where flora is rich in Baccharis species. The quantification of artepillin-C has become an important factor as an indicator of Brazilian propolis quality and the compound may be used as a chemical marker for quality control in exportating green propolis. This work was to validate the method and evaluate the content of artepillin-C from 33 samples collected in different Brazilian regions. The method used was HPLC with UV-vis detection and a reversed-phase C₁₈ column. The validation parameters studied were: linearity, accuracy, precision, quantification and detection limits. The results obtained were: detection limit = 0.0036 μg/mL, quantification limit = 0.012 μg/mL, accuracy = 0.0064 and 0.078, recovery 98-102%. Artepillin-C content varied from 0 to 11% depending on the geographical origin. Propolis from the southeast region presented the highest level of artepillin-C (5.0-11.0%). Whist that from the northeast region did not show any artepillin-C. Copyright © 2008 John Wiley & Sons, Ltd.</description><subject>5-diprenyl-4-hydroxycinnamic acid</subject><subject>artepellin-C</subject><subject>artepillin</subject><subject>Brazil</subject><subject>chemical analysis</subject><subject>chromatography</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, High Pressure Liquid - standards</subject><subject>high performance liquid chromatography</subject><subject>HPLC</subject><subject>phenolic compounds</subject><subject>Phenylpropionates - analysis</subject><subject>Phenylpropionates - chemistry</subject><subject>propolis</subject><subject>Propolis - chemistry</subject><subject>Quality Control</subject><subject>quantitative analysis</subject><subject>Reproducibility of Results</subject><subject>validation</subject><issn>0958-0344</issn><issn>1099-1565</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10F1PFDEUBuDGaGRBE38B9MpwM3raTj_mEkdZSBY1UcC7ptvpQLU7HdrZCP56S3aCV16dJufJ25MXoTcE3hEA-n60pjxq9gwtCDRNRbjgz9ECGq4qYHW9h_Zz_glQdo14ifaIogAC-AJdXJngOzO5Dm_cdBs73MeEp1uH77ZmmHzvrZl8HHDssUmTG30Ifqha7Af8IZk_Pngz4DHFMQafX6EXvQnZvZ7nAbo8_fS9PatWX5bn7cmqsnUtWaUaQXpOlaxpVzecCeWooE25iVFTO9etOZFc9BK4stIw1VkpaMFgnVp3lh2gt7vc8vHd1uVJb3y2LgQzuLjNWgKTvOQWeLyDNsWck-v1mPzGpAdNQD9Wp0t1-rG6Qg_nzO1647p_cO6qgGoHfvvgHv4bpL-2J3Pg7H2e3P2TN-mXFrLcp68_L_Xy-uOKXfy40m3xRzvfm6jNTfJZX36jQBiAEg1Iwf4CYa2OxQ</recordid><startdate>200803</startdate><enddate>200803</enddate><creator>Matsuda, Adriana Hitomi</creator><creator>de Almeida-Muradian, Ligia Bicudo</creator><general>John Wiley & Sons, Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200803</creationdate><title>Validated method for the quantification of artepillin-C in Brazilian propolis</title><author>Matsuda, Adriana Hitomi ; de Almeida-Muradian, Ligia Bicudo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4473-8961f528742d495368e262920032a4eedb51756f7058c7a38dc76242d0ce8bdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>5-diprenyl-4-hydroxycinnamic acid</topic><topic>artepellin-C</topic><topic>artepillin</topic><topic>Brazil</topic><topic>chemical analysis</topic><topic>chromatography</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, High Pressure Liquid - standards</topic><topic>high performance liquid chromatography</topic><topic>HPLC</topic><topic>phenolic compounds</topic><topic>Phenylpropionates - analysis</topic><topic>Phenylpropionates - chemistry</topic><topic>propolis</topic><topic>Propolis - chemistry</topic><topic>Quality Control</topic><topic>quantitative analysis</topic><topic>Reproducibility of Results</topic><topic>validation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuda, Adriana Hitomi</creatorcontrib><creatorcontrib>de Almeida-Muradian, Ligia Bicudo</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemical analysis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuda, Adriana Hitomi</au><au>de Almeida-Muradian, Ligia Bicudo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validated method for the quantification of artepillin-C in Brazilian propolis</atitle><jtitle>Phytochemical analysis</jtitle><addtitle>Phytochem. Anal</addtitle><date>2008-03</date><risdate>2008</risdate><volume>19</volume><issue>2</issue><spage>179</spage><epage>183</epage><pages>179-183</pages><issn>0958-0344</issn><eissn>1099-1565</eissn><abstract>Brazilian propolis contains several phenolic compounds among which 5 diprenyl-4-hydroxycinnamic acid (artepillin-C) is commonly found in areas where flora is rich in Baccharis species. The quantification of artepillin-C has become an important factor as an indicator of Brazilian propolis quality and the compound may be used as a chemical marker for quality control in exportating green propolis. This work was to validate the method and evaluate the content of artepillin-C from 33 samples collected in different Brazilian regions. The method used was HPLC with UV-vis detection and a reversed-phase C₁₈ column. The validation parameters studied were: linearity, accuracy, precision, quantification and detection limits. The results obtained were: detection limit = 0.0036 μg/mL, quantification limit = 0.012 μg/mL, accuracy = 0.0064 and 0.078, recovery 98-102%. Artepillin-C content varied from 0 to 11% depending on the geographical origin. Propolis from the southeast region presented the highest level of artepillin-C (5.0-11.0%). Whist that from the northeast region did not show any artepillin-C. Copyright © 2008 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>18200605</pmid><doi>10.1002/pca.1043</doi><tpages>5</tpages></addata></record> |
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| subjects | 5-diprenyl-4-hydroxycinnamic acid artepellin-C artepillin Brazil chemical analysis chromatography Chromatography, High Pressure Liquid - methods Chromatography, High Pressure Liquid - standards high performance liquid chromatography HPLC phenolic compounds Phenylpropionates - analysis Phenylpropionates - chemistry propolis Propolis - chemistry Quality Control quantitative analysis Reproducibility of Results validation |
| title | Validated method for the quantification of artepillin-C in Brazilian propolis |
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