Performance of a tetracycline-responsive transactivator system for regulating transgenes in the oomycete Phytophthora infestans

The oomycete genus Phytophthora includes many important plant pathogens for which extensive genome data exist, but lacking is an inducible expression system to study contributions of their genes to growth and pathogenicity. Here the adaptation of the reverse tetracycline transactivator (rtTA) system...

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Veröffentlicht in:	Current genetics 2007-05, Vol.51 (5), p.297-307
Hauptverfasser:	Judelson, Howard S, Narayan, Reena, Ah Fong, Audrey M. V, Tani, Shuji, Kim, Kyoung Su
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding sites Doxycycline Functional genomics Genes Genes, Reporter Genetic transformation Genetic Vectors Inducible promoter Microbiology Oomycete transformation Pathogenicity Pathogens Phytophthora Phytophthora - genetics Phytophthora - metabolism Phytophthora infestans Plasmids Promoter Regions, Genetic RNA, Messenger Tet-on system Tetracycline - metabolism Tetracyclines - metabolism Toxicity Transcriptional Activation Transformation, Genetic Transgenes
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