Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil
Since 1998 a cat-transmitted epidemic of sporotrichosis has been observed in Rio de Janeiro (RJ), Brazil. Besides the lymphocutaneous and fixed forms, other presentations, such as disseminated cutaneous and mucosal involvement, as well as for the first time, erythema nodosum and erythema multiforme...
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| Veröffentlicht in: | Medical mycology (Oxford) 2008-03, Vol.46 (2), p.141-151 |
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| creator | Clara Gutierrez Galhardo, Maria Maria Zancopé De Oliveira, Rosely Carlos Francesconi Do Valle, Antonio De Almeida Paes, Rodrigo Morais E Silvatavares, Patricia Monzon, Araceli Mellado, Emilia Rodriguez-Tudela, Juan L Cuenca-Estrella, Manuel |
| description | Since 1998 a cat-transmitted epidemic of sporotrichosis has been observed in Rio de Janeiro (RJ), Brazil. Besides the lymphocutaneous and fixed forms, other presentations, such as disseminated cutaneous and mucosal involvement, as well as for the first time, erythema nodosum and erythema multiforme have been reported associated with sporotrichosis. This study investigates the phenotypes and genotypes of Sporothrix schenckii isolates recovered from different clinical forms of the disease noted as part of this epidemic. A total of 88 isolates recovered from 59 cases associated with the epidemic and 29 controls (from cases in other Brazilian regions and Spain) were included in this study. In vitro susceptibility testing was conducted as part of the phenotypic analysis, while the genotypic analysis involved a DNA fingerprinting method with primer M13 and ribosomal DNA sequencing of the internal transcribed spacer (ITS). MIC values of amphotericin B, itraconazole, posaconazole, ravuconazole, and terbinafine were found to be significantly lower (P |
| doi_str_mv | 10.1080/13693780701742399 |
| format | Article |
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Besides the lymphocutaneous and fixed forms, other presentations, such as disseminated cutaneous and mucosal involvement, as well as for the first time, erythema nodosum and erythema multiforme have been reported associated with sporotrichosis. This study investigates the phenotypes and genotypes of Sporothrix schenckii isolates recovered from different clinical forms of the disease noted as part of this epidemic. A total of 88 isolates recovered from 59 cases associated with the epidemic and 29 controls (from cases in other Brazilian regions and Spain) were included in this study. In vitro susceptibility testing was conducted as part of the phenotypic analysis, while the genotypic analysis involved a DNA fingerprinting method with primer M13 and ribosomal DNA sequencing of the internal transcribed spacer (ITS). MIC values of amphotericin B, itraconazole, posaconazole, ravuconazole, and terbinafine were found to be significantly lower (P<0.01) for isolates associated with the epidemic than for control strains. No differences in MIC values were observed related to clinical forms of the infection. Fingerprinting analysis showed that RJ epidemic strains were genetically related. Although nine subtypes were found, they were not associated with specific clinical forms. Similar results were obtained with the ITS sequence analysis. These data suggest that the strains isolated from the epidemic cases of sporotrichosis in RJ all originated from a common source.</description><identifier>ISSN: 1369-3786</identifier><identifier>EISSN: 1460-2709</identifier><identifier>DOI: 10.1080/13693780701742399</identifier><identifier>PMID: 18324493</identifier><language>eng</language><publisher>UK: Informa UK Ltd</publisher><subject>Animals ; Antifungal Agents ; Brazil ; Cats ; DNA, Ribosomal Spacer - analysis ; Microbial Sensitivity Tests ; Molecular Epidemiology ; Mycological Typing Techniques ; Sporothrix - genetics ; Sporothrix - isolation & purification ; Sporothrix schenckii ; Sporotrichosis - epidemiology ; Sporotrichosis - etiology ; Sporotrichosis - pathology ; Sporotrichosis - transmission ; Zoonoses - transmission</subject><ispartof>Medical mycology (Oxford), 2008-03, Vol.46 (2), p.141-151</ispartof><rights>2008 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2008</rights><rights>2008 International Society for Human and Animal Mycology 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-403ae5194c8acd59fbd59f197a1b9f70201c1c008b35e867f1f40863840d0b7f3</citedby><cites>FETCH-LOGICAL-c419t-403ae5194c8acd59fbd59f197a1b9f70201c1c008b35e867f1f40863840d0b7f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.1080/13693780701742399$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.1080/13693780701742399$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,61221,61402</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18324493$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Clara Gutierrez Galhardo, Maria</creatorcontrib><creatorcontrib>Maria Zancopé De Oliveira, Rosely</creatorcontrib><creatorcontrib>Carlos Francesconi Do Valle, Antonio</creatorcontrib><creatorcontrib>De Almeida Paes, Rodrigo</creatorcontrib><creatorcontrib>Morais E Silvatavares, Patricia</creatorcontrib><creatorcontrib>Monzon, Araceli</creatorcontrib><creatorcontrib>Mellado, Emilia</creatorcontrib><creatorcontrib>Rodriguez-Tudela, Juan L</creatorcontrib><creatorcontrib>Cuenca-Estrella, Manuel</creatorcontrib><title>Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil</title><title>Medical mycology (Oxford)</title><addtitle>Med Mycol</addtitle><description>Since 1998 a cat-transmitted epidemic of sporotrichosis has been observed in Rio de Janeiro (RJ), Brazil. Besides the lymphocutaneous and fixed forms, other presentations, such as disseminated cutaneous and mucosal involvement, as well as for the first time, erythema nodosum and erythema multiforme have been reported associated with sporotrichosis. This study investigates the phenotypes and genotypes of Sporothrix schenckii isolates recovered from different clinical forms of the disease noted as part of this epidemic. A total of 88 isolates recovered from 59 cases associated with the epidemic and 29 controls (from cases in other Brazilian regions and Spain) were included in this study. In vitro susceptibility testing was conducted as part of the phenotypic analysis, while the genotypic analysis involved a DNA fingerprinting method with primer M13 and ribosomal DNA sequencing of the internal transcribed spacer (ITS). MIC values of amphotericin B, itraconazole, posaconazole, ravuconazole, and terbinafine were found to be significantly lower (P<0.01) for isolates associated with the epidemic than for control strains. No differences in MIC values were observed related to clinical forms of the infection. Fingerprinting analysis showed that RJ epidemic strains were genetically related. Although nine subtypes were found, they were not associated with specific clinical forms. Similar results were obtained with the ITS sequence analysis. These data suggest that the strains isolated from the epidemic cases of sporotrichosis in RJ all originated from a common source.</description><subject>Animals</subject><subject>Antifungal Agents</subject><subject>Brazil</subject><subject>Cats</subject><subject>DNA, Ribosomal Spacer - analysis</subject><subject>Microbial Sensitivity Tests</subject><subject>Molecular Epidemiology</subject><subject>Mycological Typing Techniques</subject><subject>Sporothrix - genetics</subject><subject>Sporothrix - isolation & purification</subject><subject>Sporothrix schenckii</subject><subject>Sporotrichosis - epidemiology</subject><subject>Sporotrichosis - etiology</subject><subject>Sporotrichosis - pathology</subject><subject>Sporotrichosis - transmission</subject><subject>Zoonoses - transmission</subject><issn>1369-3786</issn><issn>1460-2709</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkctu1DAUhiMEoqXwAGyQV6waehx74lisoOJWFSFxWUeOYzenOHawHYnhkXjKephBLCoBC18kf_93bJ-qekzhGYUOzihrJRMdCKCCN0zKO9Ux5S3UjQB5t-zLeV2A9qh6kNI1FEw27H51RDvWcC7ZcfXzfXBGr05FYhYczYzBhastUX4sI6Nd_ZVyJK1JmyXjgA7zliwqZxN9IsGST0uIIU8Rv5OkJ-P1V0SCKTiVTSI2hpkoolWuc1Q-zViS4-9aeidIvwQR9RQSJoKefMRARkMulDcYwyl5GdUPdA-re1a5ZB4d1pPqy-tXn8_f1pcf3rw7f3FZa05lrjkwZTZUct0pPW6kHXYTlULRQVoBDVBNNUA3sI3pWmGp5dC1rOMwwiAsO6me7r1LDN9Wk3I_Y3m9c-U6YU29ACZYS8U_QSq7IoUdSPegjiGlaGy_RJxV3PYU-l0n-1udLJknB_k6zGb8kzi0rgCneyCsy3_5nu9x9DbEWU1GuTxpFU1_Hdboy4_-JX0DymO8EQ</recordid><startdate>200803</startdate><enddate>200803</enddate><creator>Clara Gutierrez Galhardo, Maria</creator><creator>Maria Zancopé De Oliveira, Rosely</creator><creator>Carlos Francesconi Do Valle, Antonio</creator><creator>De Almeida Paes, Rodrigo</creator><creator>Morais E Silvatavares, Patricia</creator><creator>Monzon, Araceli</creator><creator>Mellado, Emilia</creator><creator>Rodriguez-Tudela, Juan L</creator><creator>Cuenca-Estrella, Manuel</creator><general>Informa UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200803</creationdate><title>Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil</title><author>Clara Gutierrez Galhardo, Maria ; Maria Zancopé De Oliveira, Rosely ; Carlos Francesconi Do Valle, Antonio ; De Almeida Paes, Rodrigo ; Morais E Silvatavares, Patricia ; Monzon, Araceli ; Mellado, Emilia ; Rodriguez-Tudela, Juan L ; Cuenca-Estrella, Manuel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-403ae5194c8acd59fbd59f197a1b9f70201c1c008b35e867f1f40863840d0b7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antifungal Agents</topic><topic>Brazil</topic><topic>Cats</topic><topic>DNA, Ribosomal Spacer - analysis</topic><topic>Microbial Sensitivity Tests</topic><topic>Molecular Epidemiology</topic><topic>Mycological Typing Techniques</topic><topic>Sporothrix - genetics</topic><topic>Sporothrix - isolation & purification</topic><topic>Sporothrix schenckii</topic><topic>Sporotrichosis - epidemiology</topic><topic>Sporotrichosis - etiology</topic><topic>Sporotrichosis - pathology</topic><topic>Sporotrichosis - transmission</topic><topic>Zoonoses - transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Clara Gutierrez Galhardo, Maria</creatorcontrib><creatorcontrib>Maria Zancopé De Oliveira, Rosely</creatorcontrib><creatorcontrib>Carlos Francesconi Do Valle, Antonio</creatorcontrib><creatorcontrib>De Almeida Paes, Rodrigo</creatorcontrib><creatorcontrib>Morais E Silvatavares, Patricia</creatorcontrib><creatorcontrib>Monzon, Araceli</creatorcontrib><creatorcontrib>Mellado, Emilia</creatorcontrib><creatorcontrib>Rodriguez-Tudela, Juan L</creatorcontrib><creatorcontrib>Cuenca-Estrella, Manuel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Medical mycology (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Clara Gutierrez Galhardo, Maria</au><au>Maria Zancopé De Oliveira, Rosely</au><au>Carlos Francesconi Do Valle, Antonio</au><au>De Almeida Paes, Rodrigo</au><au>Morais E Silvatavares, Patricia</au><au>Monzon, Araceli</au><au>Mellado, Emilia</au><au>Rodriguez-Tudela, Juan L</au><au>Cuenca-Estrella, Manuel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil</atitle><jtitle>Medical mycology (Oxford)</jtitle><addtitle>Med Mycol</addtitle><date>2008-03</date><risdate>2008</risdate><volume>46</volume><issue>2</issue><spage>141</spage><epage>151</epage><pages>141-151</pages><issn>1369-3786</issn><eissn>1460-2709</eissn><abstract>Since 1998 a cat-transmitted epidemic of sporotrichosis has been observed in Rio de Janeiro (RJ), Brazil. Besides the lymphocutaneous and fixed forms, other presentations, such as disseminated cutaneous and mucosal involvement, as well as for the first time, erythema nodosum and erythema multiforme have been reported associated with sporotrichosis. This study investigates the phenotypes and genotypes of Sporothrix schenckii isolates recovered from different clinical forms of the disease noted as part of this epidemic. A total of 88 isolates recovered from 59 cases associated with the epidemic and 29 controls (from cases in other Brazilian regions and Spain) were included in this study. In vitro susceptibility testing was conducted as part of the phenotypic analysis, while the genotypic analysis involved a DNA fingerprinting method with primer M13 and ribosomal DNA sequencing of the internal transcribed spacer (ITS). MIC values of amphotericin B, itraconazole, posaconazole, ravuconazole, and terbinafine were found to be significantly lower (P<0.01) for isolates associated with the epidemic than for control strains. No differences in MIC values were observed related to clinical forms of the infection. Fingerprinting analysis showed that RJ epidemic strains were genetically related. Although nine subtypes were found, they were not associated with specific clinical forms. Similar results were obtained with the ITS sequence analysis. These data suggest that the strains isolated from the epidemic cases of sporotrichosis in RJ all originated from a common source.</abstract><cop>UK</cop><pub>Informa UK Ltd</pub><pmid>18324493</pmid><doi>10.1080/13693780701742399</doi><tpages>11</tpages></addata></record> |
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| ispartof | Medical mycology (Oxford), 2008-03, Vol.46 (2), p.141-151 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Taylor & Francis Journals Complete |
| subjects | Animals Antifungal Agents Brazil Cats DNA, Ribosomal Spacer - analysis Microbial Sensitivity Tests Molecular Epidemiology Mycological Typing Techniques Sporothrix - genetics Sporothrix - isolation & purification Sporothrix schenckii Sporotrichosis - epidemiology Sporotrichosis - etiology Sporotrichosis - pathology Sporotrichosis - transmission Zoonoses - transmission |
| title | Molecular epidemiology and antifungal susceptibility patterns of Sporothrix schenckii isolates from a cat-transmitted epidemic of sporotrichosis in Rio de Janeiro, Brazil |
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