Improved Human Embryonic Stem Cell Embryoid Body Homogeneity and Cardiomyocyte Differentiation from a Novel V‐96 Plate Aggregation System Highlights Interline Variability

Although all human ESC (hESC) lines have similar morphology, express key pluripotency markers, and can differentiate toward primitive germ layers in vitro, the lineage‐specific developmental potential may vary between individual lines. In the current study, four hESC lines were cultured in the same...

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Veröffentlicht in:	Stem cells (Dayton, Ohio) Ohio), 2007-04, Vol.25 (4), p.929-938
Hauptverfasser:	Burridge, Paul W., Anderson, David, Priddle, Helen, Barbadillo Muñoz, Maria D., Chamberlain, Sarah, Allegrucci, Cinzia, Young, Lorraine E., Denning, Chris
Format:	Artikel
Sprache:	eng
Schlagworte:	Activin A Basic fibroblast growth factor Animals Cardiomyocytes Cell Aggregation Cell Culture Techniques - methods Cell Differentiation Cell Line Culture Media Differentiation Embryoid body Embryonic Stem Cells - cytology Embryonic Stem Cells - physiology Fibroblasts - cytology Fibroblasts - physiology Forced aggregation Human embryonic stem cells Humans Karyotyping Major Histocompatibility Complex Mice Muscle Cells - cytology Myocardium - cytology
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