Comparison of histologic techniques for the diagnosis of bovine tuberculosis in the framework of eradication programs
Correspondence: 1 Corresponding Author: Elena Bozzetta, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d' Aosta-Area Neuroscienze e Genetica-laboratorio di Immunoistopatologia, via Bologna 148, 10154 Torino, Italy, e-mail: elena.bozzetta{at}izsto.it Rapid diagnosis of tuber...
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| Veröffentlicht in: | Journal of veterinary diagnostic investigation 2008-03, Vol.20 (2), p.164-169 |
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| creator | Varello, K Pezzolato, M Mascarino, D Ingravalle, F Caramelli, M Bozzetta, E |
| description | Correspondence: 1 Corresponding Author: Elena Bozzetta, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d' Aosta-Area Neuroscienze e Genetica-laboratorio di Immunoistopatologia, via Bologna 148, 10154 Torino, Italy, e-mail: elena.bozzetta{at}izsto.it
Rapid diagnosis of tuberculosis in cattle reacting positive in antemortem assays is crucial in countries where eradication programs are operated to confirm the presence of the infection in tuberculosis-free herds. This study evaluated the accuracy of histopathologic examination by hematoxylin and eosin and Ziehl-Neelsen (ZN) staining applied in this framework, when suspected lesions are caused by low infectious doses and are detected in early stages of the disease. For this purpose, histologic methods were compared with mycobacterial culture as reference test on suspected lymph node samples from 173 cattle reacting positive in antemortem tests. Histopathology demonstrated high sensitivity (93.4%) and specificity (92.3%), while ZN sensitivity and specificity were respectively 33.9% and 100%. There was good agreement between histopathology and bacterial culture, suggesting that histopathologic examination is a reliable tool for rapid diagnosis in countries where active tuberculosis eradication programs allow the prompt identification and elimination of reactor cattle. Histopathology permits identification of typical mycobacterial lesions and its differentiation from other causes.
Key Words: Accuracy cattle eradication histopathology tuberculosis |
| doi_str_mv | 10.1177/104063870802000204 |
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Rapid diagnosis of tuberculosis in cattle reacting positive in antemortem assays is crucial in countries where eradication programs are operated to confirm the presence of the infection in tuberculosis-free herds. This study evaluated the accuracy of histopathologic examination by hematoxylin and eosin and Ziehl-Neelsen (ZN) staining applied in this framework, when suspected lesions are caused by low infectious doses and are detected in early stages of the disease. For this purpose, histologic methods were compared with mycobacterial culture as reference test on suspected lymph node samples from 173 cattle reacting positive in antemortem tests. Histopathology demonstrated high sensitivity (93.4%) and specificity (92.3%), while ZN sensitivity and specificity were respectively 33.9% and 100%. There was good agreement between histopathology and bacterial culture, suggesting that histopathologic examination is a reliable tool for rapid diagnosis in countries where active tuberculosis eradication programs allow the prompt identification and elimination of reactor cattle. Histopathology permits identification of typical mycobacterial lesions and its differentiation from other causes.
Key Words: Accuracy cattle eradication histopathology tuberculosis</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063870802000204</identifier><identifier>PMID: 18319428</identifier><language>eng</language><publisher>Los Angeles, CA: J Vet Diagn Invest</publisher><subject>accuracy ; animal pathogenic bacteria ; Animals ; bovine tuberculosis ; Cattle ; diagnostic techniques ; differential staining ; disease control ; disease detection ; disease diagnosis ; disease surveillance ; DNA Transposable Elements - genetics ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Histocytochemistry - methods ; Histocytochemistry - veterinary ; histopathology ; lymph nodes ; Lymph Nodes - virology ; Mycobacterium bovis ; Mycobacterium bovis - genetics ; Mycobacterium bovis - isolation & purification ; pathogen eradication ; Polymerase Chain Reaction - veterinary ; Predictive Value of Tests ; reliability ; RNA, Ribosomal, 16S - chemistry ; RNA, Ribosomal, 16S - genetics ; Sensitivity and Specificity ; test sensitivity ; test specificity ; Tuberculosis, Bovine - diagnosis ; Tuberculosis, Bovine - prevention & control ; Tuberculosis, Bovine - virology</subject><ispartof>Journal of veterinary diagnostic investigation, 2008-03, Vol.20 (2), p.164-169</ispartof><rights>2008 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-f747c0feeb06060af466c98cfde80e99676188e2c0f4175b4ecd6533982fa35d3</citedby><cites>FETCH-LOGICAL-c396t-f747c0feeb06060af466c98cfde80e99676188e2c0f4175b4ecd6533982fa35d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/104063870802000204$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/104063870802000204$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18319428$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Varello, K</creatorcontrib><creatorcontrib>Pezzolato, M</creatorcontrib><creatorcontrib>Mascarino, D</creatorcontrib><creatorcontrib>Ingravalle, F</creatorcontrib><creatorcontrib>Caramelli, M</creatorcontrib><creatorcontrib>Bozzetta, E</creatorcontrib><title>Comparison of histologic techniques for the diagnosis of bovine tuberculosis in the framework of eradication programs</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Correspondence: 1 Corresponding Author: Elena Bozzetta, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d' Aosta-Area Neuroscienze e Genetica-laboratorio di Immunoistopatologia, via Bologna 148, 10154 Torino, Italy, e-mail: elena.bozzetta{at}izsto.it
Rapid diagnosis of tuberculosis in cattle reacting positive in antemortem assays is crucial in countries where eradication programs are operated to confirm the presence of the infection in tuberculosis-free herds. This study evaluated the accuracy of histopathologic examination by hematoxylin and eosin and Ziehl-Neelsen (ZN) staining applied in this framework, when suspected lesions are caused by low infectious doses and are detected in early stages of the disease. For this purpose, histologic methods were compared with mycobacterial culture as reference test on suspected lymph node samples from 173 cattle reacting positive in antemortem tests. Histopathology demonstrated high sensitivity (93.4%) and specificity (92.3%), while ZN sensitivity and specificity were respectively 33.9% and 100%. There was good agreement between histopathology and bacterial culture, suggesting that histopathologic examination is a reliable tool for rapid diagnosis in countries where active tuberculosis eradication programs allow the prompt identification and elimination of reactor cattle. Histopathology permits identification of typical mycobacterial lesions and its differentiation from other causes.
Key Words: Accuracy cattle eradication histopathology tuberculosis</description><subject>accuracy</subject><subject>animal pathogenic bacteria</subject><subject>Animals</subject><subject>bovine tuberculosis</subject><subject>Cattle</subject><subject>diagnostic techniques</subject><subject>differential staining</subject><subject>disease control</subject><subject>disease detection</subject><subject>disease diagnosis</subject><subject>disease surveillance</subject><subject>DNA Transposable Elements - genetics</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Histocytochemistry - methods</subject><subject>Histocytochemistry - veterinary</subject><subject>histopathology</subject><subject>lymph nodes</subject><subject>Lymph Nodes - virology</subject><subject>Mycobacterium bovis</subject><subject>Mycobacterium bovis - genetics</subject><subject>Mycobacterium bovis - isolation & purification</subject><subject>pathogen eradication</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Predictive Value of Tests</subject><subject>reliability</subject><subject>RNA, Ribosomal, 16S - chemistry</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sensitivity and Specificity</subject><subject>test sensitivity</subject><subject>test specificity</subject><subject>Tuberculosis, Bovine - diagnosis</subject><subject>Tuberculosis, Bovine - prevention & control</subject><subject>Tuberculosis, Bovine - virology</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUtv1DAUhS0Eog_4Aywgq-5C_YofSzSCFqlSF9C15TjXiYckHuykFf--TmckFkjIsmzZ3z3H9xihDwR_JkTKa4I5FkxJrDDFuEz-Cp0TzVnNNROvy74A9UacoYuc9xg3tJHkLTojihWOqnO07uJ0sCnkOFfRV0PISxxjH1y1gBvm8HuFXPmYqmWAqgu2n2MOeUPb-BhmqJa1heTW8eU4zC-cT3aCp5h-bRwk2wVnl1AcDin25S6_Q2-8HTO8P62X6OHb15-72_ru_ub77std7ZgWS-0llw57gBaLMqznQjitnO9AYdBaSEGUAloYTmTTcnCdaBjTinrLmo5doqujbjHeOlnMFLKDcbQzxDUbiZmkDdUFpEfQpZhzAm8OKUw2_TEEmy1s82_YpejjSX1tJ-j-lpzSLcD1Eci2B7OPa5pLt_-XPD14CP3wFBKYPNlxLAbU7B-7QLGhhogN_HQEvY3G9uUDzcMPignDWFNOuGDPRnWfxg</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Varello, K</creator><creator>Pezzolato, M</creator><creator>Mascarino, D</creator><creator>Ingravalle, F</creator><creator>Caramelli, M</creator><creator>Bozzetta, E</creator><general>J Vet Diagn Invest</general><general>SAGE Publications</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080301</creationdate><title>Comparison of histologic techniques for the diagnosis of bovine tuberculosis in the framework of eradication programs</title><author>Varello, K ; Pezzolato, M ; Mascarino, D ; Ingravalle, F ; Caramelli, M ; Bozzetta, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c396t-f747c0feeb06060af466c98cfde80e99676188e2c0f4175b4ecd6533982fa35d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>accuracy</topic><topic>animal pathogenic bacteria</topic><topic>Animals</topic><topic>bovine tuberculosis</topic><topic>Cattle</topic><topic>diagnostic techniques</topic><topic>differential staining</topic><topic>disease control</topic><topic>disease detection</topic><topic>disease diagnosis</topic><topic>disease surveillance</topic><topic>DNA Transposable Elements - genetics</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Histocytochemistry - methods</topic><topic>Histocytochemistry - veterinary</topic><topic>histopathology</topic><topic>lymph nodes</topic><topic>Lymph Nodes - virology</topic><topic>Mycobacterium bovis</topic><topic>Mycobacterium bovis - genetics</topic><topic>Mycobacterium bovis - isolation & purification</topic><topic>pathogen eradication</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Predictive Value of Tests</topic><topic>reliability</topic><topic>RNA, Ribosomal, 16S - chemistry</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sensitivity and Specificity</topic><topic>test sensitivity</topic><topic>test specificity</topic><topic>Tuberculosis, Bovine - diagnosis</topic><topic>Tuberculosis, Bovine - prevention & control</topic><topic>Tuberculosis, Bovine - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Varello, K</creatorcontrib><creatorcontrib>Pezzolato, M</creatorcontrib><creatorcontrib>Mascarino, D</creatorcontrib><creatorcontrib>Ingravalle, F</creatorcontrib><creatorcontrib>Caramelli, M</creatorcontrib><creatorcontrib>Bozzetta, E</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Varello, K</au><au>Pezzolato, M</au><au>Mascarino, D</au><au>Ingravalle, F</au><au>Caramelli, M</au><au>Bozzetta, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of histologic techniques for the diagnosis of bovine tuberculosis in the framework of eradication programs</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>20</volume><issue>2</issue><spage>164</spage><epage>169</epage><pages>164-169</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Correspondence: 1 Corresponding Author: Elena Bozzetta, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d' Aosta-Area Neuroscienze e Genetica-laboratorio di Immunoistopatologia, via Bologna 148, 10154 Torino, Italy, e-mail: elena.bozzetta{at}izsto.it
Rapid diagnosis of tuberculosis in cattle reacting positive in antemortem assays is crucial in countries where eradication programs are operated to confirm the presence of the infection in tuberculosis-free herds. This study evaluated the accuracy of histopathologic examination by hematoxylin and eosin and Ziehl-Neelsen (ZN) staining applied in this framework, when suspected lesions are caused by low infectious doses and are detected in early stages of the disease. For this purpose, histologic methods were compared with mycobacterial culture as reference test on suspected lymph node samples from 173 cattle reacting positive in antemortem tests. Histopathology demonstrated high sensitivity (93.4%) and specificity (92.3%), while ZN sensitivity and specificity were respectively 33.9% and 100%. There was good agreement between histopathology and bacterial culture, suggesting that histopathologic examination is a reliable tool for rapid diagnosis in countries where active tuberculosis eradication programs allow the prompt identification and elimination of reactor cattle. Histopathology permits identification of typical mycobacterial lesions and its differentiation from other causes.
Key Words: Accuracy cattle eradication histopathology tuberculosis</abstract><cop>Los Angeles, CA</cop><pub>J Vet Diagn Invest</pub><pmid>18319428</pmid><doi>10.1177/104063870802000204</doi><tpages>6</tpages></addata></record> |
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| subjects | accuracy animal pathogenic bacteria Animals bovine tuberculosis Cattle diagnostic techniques differential staining disease control disease detection disease diagnosis disease surveillance DNA Transposable Elements - genetics DNA, Bacterial - chemistry DNA, Bacterial - genetics Histocytochemistry - methods Histocytochemistry - veterinary histopathology lymph nodes Lymph Nodes - virology Mycobacterium bovis Mycobacterium bovis - genetics Mycobacterium bovis - isolation & purification pathogen eradication Polymerase Chain Reaction - veterinary Predictive Value of Tests reliability RNA, Ribosomal, 16S - chemistry RNA, Ribosomal, 16S - genetics Sensitivity and Specificity test sensitivity test specificity Tuberculosis, Bovine - diagnosis Tuberculosis, Bovine - prevention & control Tuberculosis, Bovine - virology |
| title | Comparison of histologic techniques for the diagnosis of bovine tuberculosis in the framework of eradication programs |
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