ANP stimulates hepatocyte Ca2+ efflux via plasma membrane recruitment of PKGIalpha
In rat hepatocytes, atrial natriuretic peptide (ANP) elevates cGMP through activation of particulate guanylyl cyclase and attenuates Ca(2+) signals by stimulating net plasma membrane Ca(2+) efflux. We show here that ANP-stimulated hepatocyte Ca(2+) efflux is mediated by protein kinase G (PKG) isotyp...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2008-04, Vol.368 (4), p.965-970 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Stratton, Rebecca C Squires, Paul E Green, Anne K |
| description | In rat hepatocytes, atrial natriuretic peptide (ANP) elevates cGMP through activation of particulate guanylyl cyclase and attenuates Ca(2+) signals by stimulating net plasma membrane Ca(2+) efflux. We show here that ANP-stimulated hepatocyte Ca(2+) efflux is mediated by protein kinase G (PKG) isotype I. Furthermore, we show that ANP recruits endogenous PKGIalpha, but not PKGIbeta, to the plasma membrane. These effects are mimicked by 8-bromo-cGMP, but not by the soluble guanylyl cyclase activators, sodium nitroprusside and YC-1. We propose that ANP, through localized cGMP elevation, promotes plasma membrane recruitment of PKGIalpha, which, in turn, stimulates Ca(2+) efflux. |
| doi_str_mv | 10.1016/j.bbrc.2008.02.030 |
| format | Article |
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| ispartof | Biochemical and biophysical research communications, 2008-04, Vol.368 (4), p.965-970 |
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| language | eng |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Atrial Natriuretic Factor - pharmacology Calcium - metabolism Cell Membrane - metabolism Cyclic GMP - analogs & derivatives Cyclic GMP - pharmacology Cyclic GMP-Dependent Protein Kinase Type I Cyclic GMP-Dependent Protein Kinases - physiology Hepatocytes - drug effects Hepatocytes - metabolism Indazoles - pharmacology Male Nitroprusside - pharmacology Rats Rats, Wistar Thionucleotides - pharmacology |
| title | ANP stimulates hepatocyte Ca2+ efflux via plasma membrane recruitment of PKGIalpha |
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