Enzyme-Assisted Aqueous Extraction of Oleosomes from Soybeans (Glycine max)
Oleosomes, with their unique structural proteins, the oleosins, are known to be useful in cosmetics and other emulsion applications. A procedure to fractionate intact oleosomes to produce soybean oil without the use of organic solvents was investigated. Process parameters, enzyme treatment, filtrati...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 2008-03, Vol.56 (5), p.1766-1771 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1771 |
|---|---|
| container_issue | 5 |
| container_start_page | 1766 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 56 |
| creator | Kapchie, Virginie N Wei, Daijing Hauck, Catherine Murphy, Patricia A |
| description | Oleosomes, with their unique structural proteins, the oleosins, are known to be useful in cosmetics and other emulsion applications. A procedure to fractionate intact oleosomes to produce soybean oil without the use of organic solvents was investigated. Process parameters, enzyme treatment, filtration, cell lysis, and centrifugation, were studied. Successive extractions of the residue, eliminating the filtration step, pressurization, or ultrasonication of soybean flour prior to enzyme treatment and enzyme treatment on the residue, were the key steps. A mixture of Multifect Pectinase FE, Cellulase A, and Multifect CX 13L in equal proportion gave 36.42−63.23% of the total soybean oil from oleosomes, respectively, for 45 and 180 s of blending time, compared to the conventional method with lower yields (34.24 and 28.65%, respectively, for 45 and 180 s of blending time). Three successive extractions of the residue increased the oil yield to a maximum of 84.65% of the total soybean oil recovered in intact oleosomes. The percentage of lipid in the supernatant fraction decreased to a minimum value of 0.33% with the use of the enzymes at a 3% dosage. The results are considered to be useful for developing large-scale and efficient extraction of oleosomes from soybean. |
| doi_str_mv | 10.1021/jf0721390 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70364950</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70364950</sourcerecordid><originalsourceid>FETCH-LOGICAL-a405t-9094e59bb4e32967b097e37ce361e5c108564a157f3c7b0998af68f2dfd9ad543</originalsourceid><addsrcrecordid>eNptkUFv0zAYhi3ExLrBgT8AuTCxQ-D74tiOj93WDUSlIXUT3CzHsVFKEg87lVp-PY5adRdOPryPXr9-TMhbhE8IBX5eOxAFUgkvyAxZATlDrF6SGaQwrxjHU3IW4xoAKibgFTnFqmDIAGfk22L4u-ttPo-xjaNtsvmfjfWbmC22Y9BmbP2QeZfdd9ZH39uYueD7bOV3tdVDzD7edTvTDjbr9fbyNTlxuov2zeE8J4-3i4frL_ny_u7r9XyZ6xLYmEuQpWWyrktLC8lFDVJYKoylHC0zmEbyUiMTjpoplJV2vHJF4xqpG1bSc3Kx730KPq2No-rbaGzX6WGargRQXkoGCbzcgyb4GIN16im0vQ47haAmc-poLrHvDqWburfNM3lQlYAPB0BHozsX9GDaeOQKQF4Bm7h8z00-t8dch9-KCyqYevi-Uku2urkqf6D6mfj3e95pr_SvkDofV6mNpt8SWCB_vlmbqNZ-E4Zk9z9P-AdmN5gF</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70364950</pqid></control><display><type>article</type><title>Enzyme-Assisted Aqueous Extraction of Oleosomes from Soybeans (Glycine max)</title><source>ACS Publications</source><source>MEDLINE</source><creator>Kapchie, Virginie N ; Wei, Daijing ; Hauck, Catherine ; Murphy, Patricia A</creator><creatorcontrib>Kapchie, Virginie N ; Wei, Daijing ; Hauck, Catherine ; Murphy, Patricia A</creatorcontrib><description>Oleosomes, with their unique structural proteins, the oleosins, are known to be useful in cosmetics and other emulsion applications. A procedure to fractionate intact oleosomes to produce soybean oil without the use of organic solvents was investigated. Process parameters, enzyme treatment, filtration, cell lysis, and centrifugation, were studied. Successive extractions of the residue, eliminating the filtration step, pressurization, or ultrasonication of soybean flour prior to enzyme treatment and enzyme treatment on the residue, were the key steps. A mixture of Multifect Pectinase FE, Cellulase A, and Multifect CX 13L in equal proportion gave 36.42−63.23% of the total soybean oil from oleosomes, respectively, for 45 and 180 s of blending time, compared to the conventional method with lower yields (34.24 and 28.65%, respectively, for 45 and 180 s of blending time). Three successive extractions of the residue increased the oil yield to a maximum of 84.65% of the total soybean oil recovered in intact oleosomes. The percentage of lipid in the supernatant fraction decreased to a minimum value of 0.33% with the use of the enzymes at a 3% dosage. The results are considered to be useful for developing large-scale and efficient extraction of oleosomes from soybean.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf0721390</identifier><identifier>PMID: 18251501</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Biological and medical sciences ; cellulase ; Cellulase - metabolism ; enzymatic treatment ; enzyme-assisted aqueous extraction ; extraction ; Fat industries ; Flour ; food analysis ; Food Chemistry/Biochemistry ; food composition ; Food engineering ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; Glycine max ; Glycine max - chemistry ; Glycoside Hydrolases - metabolism ; hemicellulase ; lipid bodies ; lipid content ; Membrane Proteins ; oil recovery ; pectinase ; plant extracts ; Plant Proteins ; Polygalacturonase - metabolism ; Soybean Oil ; Soybean oleosomes ; Soybean Proteins - isolation & purification ; Soybean Proteins - metabolism ; soybeans ; structural proteins</subject><ispartof>Journal of agricultural and food chemistry, 2008-03, Vol.56 (5), p.1766-1771</ispartof><rights>Copyright © 2008 American Chemical Society</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a405t-9094e59bb4e32967b097e37ce361e5c108564a157f3c7b0998af68f2dfd9ad543</citedby><cites>FETCH-LOGICAL-a405t-9094e59bb4e32967b097e37ce361e5c108564a157f3c7b0998af68f2dfd9ad543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf0721390$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf0721390$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20168051$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18251501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kapchie, Virginie N</creatorcontrib><creatorcontrib>Wei, Daijing</creatorcontrib><creatorcontrib>Hauck, Catherine</creatorcontrib><creatorcontrib>Murphy, Patricia A</creatorcontrib><title>Enzyme-Assisted Aqueous Extraction of Oleosomes from Soybeans (Glycine max)</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Oleosomes, with their unique structural proteins, the oleosins, are known to be useful in cosmetics and other emulsion applications. A procedure to fractionate intact oleosomes to produce soybean oil without the use of organic solvents was investigated. Process parameters, enzyme treatment, filtration, cell lysis, and centrifugation, were studied. Successive extractions of the residue, eliminating the filtration step, pressurization, or ultrasonication of soybean flour prior to enzyme treatment and enzyme treatment on the residue, were the key steps. A mixture of Multifect Pectinase FE, Cellulase A, and Multifect CX 13L in equal proportion gave 36.42−63.23% of the total soybean oil from oleosomes, respectively, for 45 and 180 s of blending time, compared to the conventional method with lower yields (34.24 and 28.65%, respectively, for 45 and 180 s of blending time). Three successive extractions of the residue increased the oil yield to a maximum of 84.65% of the total soybean oil recovered in intact oleosomes. The percentage of lipid in the supernatant fraction decreased to a minimum value of 0.33% with the use of the enzymes at a 3% dosage. The results are considered to be useful for developing large-scale and efficient extraction of oleosomes from soybean.</description><subject>Biological and medical sciences</subject><subject>cellulase</subject><subject>Cellulase - metabolism</subject><subject>enzymatic treatment</subject><subject>enzyme-assisted aqueous extraction</subject><subject>extraction</subject><subject>Fat industries</subject><subject>Flour</subject><subject>food analysis</subject><subject>Food Chemistry/Biochemistry</subject><subject>food composition</subject><subject>Food engineering</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Glycine max</subject><subject>Glycine max - chemistry</subject><subject>Glycoside Hydrolases - metabolism</subject><subject>hemicellulase</subject><subject>lipid bodies</subject><subject>lipid content</subject><subject>Membrane Proteins</subject><subject>oil recovery</subject><subject>pectinase</subject><subject>plant extracts</subject><subject>Plant Proteins</subject><subject>Polygalacturonase - metabolism</subject><subject>Soybean Oil</subject><subject>Soybean oleosomes</subject><subject>Soybean Proteins - isolation & purification</subject><subject>Soybean Proteins - metabolism</subject><subject>soybeans</subject><subject>structural proteins</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUFv0zAYhi3ExLrBgT8AuTCxQ-D74tiOj93WDUSlIXUT3CzHsVFKEg87lVp-PY5adRdOPryPXr9-TMhbhE8IBX5eOxAFUgkvyAxZATlDrF6SGaQwrxjHU3IW4xoAKibgFTnFqmDIAGfk22L4u-ttPo-xjaNtsvmfjfWbmC22Y9BmbP2QeZfdd9ZH39uYueD7bOV3tdVDzD7edTvTDjbr9fbyNTlxuov2zeE8J4-3i4frL_ny_u7r9XyZ6xLYmEuQpWWyrktLC8lFDVJYKoylHC0zmEbyUiMTjpoplJV2vHJF4xqpG1bSc3Kx730KPq2No-rbaGzX6WGargRQXkoGCbzcgyb4GIN16im0vQ47haAmc-poLrHvDqWburfNM3lQlYAPB0BHozsX9GDaeOQKQF4Bm7h8z00-t8dch9-KCyqYevi-Uku2urkqf6D6mfj3e95pr_SvkDofV6mNpt8SWCB_vlmbqNZ-E4Zk9z9P-AdmN5gF</recordid><startdate>20080312</startdate><enddate>20080312</enddate><creator>Kapchie, Virginie N</creator><creator>Wei, Daijing</creator><creator>Hauck, Catherine</creator><creator>Murphy, Patricia A</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080312</creationdate><title>Enzyme-Assisted Aqueous Extraction of Oleosomes from Soybeans (Glycine max)</title><author>Kapchie, Virginie N ; Wei, Daijing ; Hauck, Catherine ; Murphy, Patricia A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a405t-9094e59bb4e32967b097e37ce361e5c108564a157f3c7b0998af68f2dfd9ad543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>cellulase</topic><topic>Cellulase - metabolism</topic><topic>enzymatic treatment</topic><topic>enzyme-assisted aqueous extraction</topic><topic>extraction</topic><topic>Fat industries</topic><topic>Flour</topic><topic>food analysis</topic><topic>Food Chemistry/Biochemistry</topic><topic>food composition</topic><topic>Food engineering</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Glycine max</topic><topic>Glycine max - chemistry</topic><topic>Glycoside Hydrolases - metabolism</topic><topic>hemicellulase</topic><topic>lipid bodies</topic><topic>lipid content</topic><topic>Membrane Proteins</topic><topic>oil recovery</topic><topic>pectinase</topic><topic>plant extracts</topic><topic>Plant Proteins</topic><topic>Polygalacturonase - metabolism</topic><topic>Soybean Oil</topic><topic>Soybean oleosomes</topic><topic>Soybean Proteins - isolation & purification</topic><topic>Soybean Proteins - metabolism</topic><topic>soybeans</topic><topic>structural proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kapchie, Virginie N</creatorcontrib><creatorcontrib>Wei, Daijing</creatorcontrib><creatorcontrib>Hauck, Catherine</creatorcontrib><creatorcontrib>Murphy, Patricia A</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kapchie, Virginie N</au><au>Wei, Daijing</au><au>Hauck, Catherine</au><au>Murphy, Patricia A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme-Assisted Aqueous Extraction of Oleosomes from Soybeans (Glycine max)</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2008-03-12</date><risdate>2008</risdate><volume>56</volume><issue>5</issue><spage>1766</spage><epage>1771</epage><pages>1766-1771</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Oleosomes, with their unique structural proteins, the oleosins, are known to be useful in cosmetics and other emulsion applications. A procedure to fractionate intact oleosomes to produce soybean oil without the use of organic solvents was investigated. Process parameters, enzyme treatment, filtration, cell lysis, and centrifugation, were studied. Successive extractions of the residue, eliminating the filtration step, pressurization, or ultrasonication of soybean flour prior to enzyme treatment and enzyme treatment on the residue, were the key steps. A mixture of Multifect Pectinase FE, Cellulase A, and Multifect CX 13L in equal proportion gave 36.42−63.23% of the total soybean oil from oleosomes, respectively, for 45 and 180 s of blending time, compared to the conventional method with lower yields (34.24 and 28.65%, respectively, for 45 and 180 s of blending time). Three successive extractions of the residue increased the oil yield to a maximum of 84.65% of the total soybean oil recovered in intact oleosomes. The percentage of lipid in the supernatant fraction decreased to a minimum value of 0.33% with the use of the enzymes at a 3% dosage. The results are considered to be useful for developing large-scale and efficient extraction of oleosomes from soybean.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>18251501</pmid><doi>10.1021/jf0721390</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2008-03, Vol.56 (5), p.1766-1771 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70364950 |
| source | ACS Publications; MEDLINE |
| subjects | Biological and medical sciences cellulase Cellulase - metabolism enzymatic treatment enzyme-assisted aqueous extraction extraction Fat industries Flour food analysis Food Chemistry/Biochemistry food composition Food engineering Food industries Fundamental and applied biological sciences. Psychology General aspects Glycine max Glycine max - chemistry Glycoside Hydrolases - metabolism hemicellulase lipid bodies lipid content Membrane Proteins oil recovery pectinase plant extracts Plant Proteins Polygalacturonase - metabolism Soybean Oil Soybean oleosomes Soybean Proteins - isolation & purification Soybean Proteins - metabolism soybeans structural proteins |
| title | Enzyme-Assisted Aqueous Extraction of Oleosomes from Soybeans (Glycine max) |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T22%3A16%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enzyme-Assisted%20Aqueous%20Extraction%20of%20Oleosomes%20from%20Soybeans%20(Glycine%20max)&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Kapchie,%20Virginie%20N&rft.date=2008-03-12&rft.volume=56&rft.issue=5&rft.spage=1766&rft.epage=1771&rft.pages=1766-1771&rft.issn=0021-8561&rft.eissn=1520-5118&rft.coden=JAFCAU&rft_id=info:doi/10.1021/jf0721390&rft_dat=%3Cproquest_cross%3E70364950%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70364950&rft_id=info:pmid/18251501&rfr_iscdi=true |