Spatiotemporal quantification of tumor necrosis factor-alpha and interleukin-10 after crush injury in rat sciatic nerve utilizing immunohistochemistry
The purpose of this study was to investigate quantitatively the longitudinal temporal, spatial changes of the tumor necrosis factor-alpha (TNF) and interleukin-10 (IL-10) immunopositive cells during Wallerian degeneration and the following regeneration after crush injury in rat sciatic nerve using i...
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| Veröffentlicht in: | Neuroscience letters 2007-04, Vol.417 (1), p.55-60 |
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| creator | Sawada, Tomokazu Sano, Michio Omura, Takao Omura, Kumiko Hasegawa, Tomohiko Funahashi, Shinji Nagano, Akira |
| description | The purpose of this study was to investigate quantitatively the longitudinal temporal, spatial changes of the tumor necrosis factor-alpha (TNF) and interleukin-10 (IL-10) immunopositive cells during Wallerian degeneration and the following regeneration after crush injury in rat sciatic nerve using immunohistochemistry and enzyme linked immunosorbent assay (ELISA). The number of TNF-immunopositive cells reached its peak and increased significantly in all the segments distal to the crush site 3 days after injury. On Day 7, TNF-immunopositive cells decreased in all the segments distal to the crush site, and a significant decrease was observed 14 days after injury. From Day 21 to Day 56, there were no significant differences in the numbers of TNF-immunopositive cells. The average size of TNF immunopossitive cells became significantly larger with degeneration. The number of IL-10-immunopositive cells decreases significantly 1 day after crush injury. IL-10-immunopositive cells increased on Day 3, returning to control levels. Seven days after injury, a significant increase in the number of IL-10-immunopositive cells was observed. There was also no significant difference in the number of IL-10-immunopositive cells beyond Day 14 except for a part of distal segments. The number of IL-10-immunopositive cells showed no significant differences in all the segments on Day 56. The protein levels of IL-10 measured by ELISA were similar to the result of immunohistochemistry. These results suggest that the significant change in IL-10 occurred prior to the significant change in TNF and that IL-10 may be the key to the change in TNF. |
| doi_str_mv | 10.1016/j.neulet.2007.02.028 |
| format | Article |
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The number of TNF-immunopositive cells reached its peak and increased significantly in all the segments distal to the crush site 3 days after injury. On Day 7, TNF-immunopositive cells decreased in all the segments distal to the crush site, and a significant decrease was observed 14 days after injury. From Day 21 to Day 56, there were no significant differences in the numbers of TNF-immunopositive cells. The average size of TNF immunopossitive cells became significantly larger with degeneration. The number of IL-10-immunopositive cells decreases significantly 1 day after crush injury. IL-10-immunopositive cells increased on Day 3, returning to control levels. Seven days after injury, a significant increase in the number of IL-10-immunopositive cells was observed. There was also no significant difference in the number of IL-10-immunopositive cells beyond Day 14 except for a part of distal segments. The number of IL-10-immunopositive cells showed no significant differences in all the segments on Day 56. The protein levels of IL-10 measured by ELISA were similar to the result of immunohistochemistry. These results suggest that the significant change in IL-10 occurred prior to the significant change in TNF and that IL-10 may be the key to the change in TNF.</description><identifier>ISSN: 0304-3940</identifier><identifier>EISSN: 1872-7972</identifier><identifier>DOI: 10.1016/j.neulet.2007.02.028</identifier><identifier>PMID: 17336456</identifier><identifier>CODEN: NELED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Animals ; Axons - immunology ; Axons - metabolism ; Axons - pathology ; Biological and medical sciences ; Biomarkers - analysis ; Biomarkers - metabolism ; Blood–nerve barrier ; Cell Count ; Cell Size ; Enzyme-Linked Immunosorbent Assay ; Female ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Interleukin-10 ; Interleukin-10 - analysis ; Interleukin-10 - immunology ; Interleukin-10 - metabolism ; Macrophage ; Macrophages - immunology ; Macrophages - metabolism ; Rats ; Rats, Sprague-Dawley ; Schwann Cells - immunology ; Schwann Cells - metabolism ; Sciatic Nerve - immunology ; Sciatic Nerve - metabolism ; Sciatic Nerve - physiopathology ; Sciatic Neuropathy - immunology ; Sciatic Neuropathy - metabolism ; Sciatic Neuropathy - physiopathology ; Time Factors ; Tumor necrosis factor-alpha ; Tumor Necrosis Factor-alpha - analysis ; Tumor Necrosis Factor-alpha - immunology ; Tumor Necrosis Factor-alpha - metabolism ; Up-Regulation - immunology ; Vertebrates: nervous system and sense organs ; Wallerian degeneration ; Wallerian Degeneration - immunology ; Wallerian Degeneration - metabolism ; Wallerian Degeneration - physiopathology</subject><ispartof>Neuroscience letters, 2007-04, Vol.417 (1), p.55-60</ispartof><rights>2007 Elsevier Ireland Ltd</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-dd262c67b2a694526f360fd3a7f37fe6579ae16a87e744df23c33a89d1be5d1e3</citedby><cites>FETCH-LOGICAL-c502t-dd262c67b2a694526f360fd3a7f37fe6579ae16a87e744df23c33a89d1be5d1e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0304394007001826$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18702146$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17336456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sawada, Tomokazu</creatorcontrib><creatorcontrib>Sano, Michio</creatorcontrib><creatorcontrib>Omura, Takao</creatorcontrib><creatorcontrib>Omura, Kumiko</creatorcontrib><creatorcontrib>Hasegawa, Tomohiko</creatorcontrib><creatorcontrib>Funahashi, Shinji</creatorcontrib><creatorcontrib>Nagano, Akira</creatorcontrib><title>Spatiotemporal quantification of tumor necrosis factor-alpha and interleukin-10 after crush injury in rat sciatic nerve utilizing immunohistochemistry</title><title>Neuroscience letters</title><addtitle>Neurosci Lett</addtitle><description>The purpose of this study was to investigate quantitatively the longitudinal temporal, spatial changes of the tumor necrosis factor-alpha (TNF) and interleukin-10 (IL-10) immunopositive cells during Wallerian degeneration and the following regeneration after crush injury in rat sciatic nerve using immunohistochemistry and enzyme linked immunosorbent assay (ELISA). The number of TNF-immunopositive cells reached its peak and increased significantly in all the segments distal to the crush site 3 days after injury. On Day 7, TNF-immunopositive cells decreased in all the segments distal to the crush site, and a significant decrease was observed 14 days after injury. From Day 21 to Day 56, there were no significant differences in the numbers of TNF-immunopositive cells. The average size of TNF immunopossitive cells became significantly larger with degeneration. The number of IL-10-immunopositive cells decreases significantly 1 day after crush injury. IL-10-immunopositive cells increased on Day 3, returning to control levels. Seven days after injury, a significant increase in the number of IL-10-immunopositive cells was observed. There was also no significant difference in the number of IL-10-immunopositive cells beyond Day 14 except for a part of distal segments. The number of IL-10-immunopositive cells showed no significant differences in all the segments on Day 56. The protein levels of IL-10 measured by ELISA were similar to the result of immunohistochemistry. These results suggest that the significant change in IL-10 occurred prior to the significant change in TNF and that IL-10 may be the key to the change in TNF.</description><subject>Animals</subject><subject>Axons - immunology</subject><subject>Axons - metabolism</subject><subject>Axons - pathology</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - analysis</subject><subject>Biomarkers - metabolism</subject><subject>Blood–nerve barrier</subject><subject>Cell Count</subject><subject>Cell Size</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Interleukin-10</subject><subject>Interleukin-10 - analysis</subject><subject>Interleukin-10 - immunology</subject><subject>Interleukin-10 - metabolism</subject><subject>Macrophage</subject><subject>Macrophages - immunology</subject><subject>Macrophages - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Schwann Cells - immunology</subject><subject>Schwann Cells - metabolism</subject><subject>Sciatic Nerve - immunology</subject><subject>Sciatic Nerve - metabolism</subject><subject>Sciatic Nerve - physiopathology</subject><subject>Sciatic Neuropathy - immunology</subject><subject>Sciatic Neuropathy - metabolism</subject><subject>Sciatic Neuropathy - physiopathology</subject><subject>Time Factors</subject><subject>Tumor necrosis factor-alpha</subject><subject>Tumor Necrosis Factor-alpha - analysis</subject><subject>Tumor Necrosis Factor-alpha - immunology</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><subject>Up-Regulation - immunology</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>Wallerian degeneration</subject><subject>Wallerian Degeneration - immunology</subject><subject>Wallerian Degeneration - metabolism</subject><subject>Wallerian Degeneration - physiopathology</subject><issn>0304-3940</issn><issn>1872-7972</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuKFDEUhoMoTjv6BiLZ6K7aXKqSqo0gw3iBARfqOqSTEzttVdKTy0D7ID6vKbphdsKBQ5Lv_Cf8P0KvKdlSQsX7wzZAnaFsGSFyS1ir8Qna0FGyTk6SPUUbwknf8aknV-hFzgdCyECH_jm6opJz0Q9ig_5-P-riY4HlGJOe8X3VoXjnzXobcHS41CUmHMCkmH3GTpsSU6fn415jHSz2oUCaof72oaMEa9eO2KSa9-3pUNOpNZx0wdn4JmqaVHoAXIuf_R8ffmG_LDXEvc8lmj0srafTS_TM6TnDq0u_Rj8_3f64-dLdffv89ebjXWcGwkpnLRPMCLljWkz9wITjgjjLtXRcOhCDnDRQoUcJsu-tY9xwrsfJ0h0MlgK_Ru_OuscU7yvkotp-A_OsA8SalSRcUDLJBvZncLUhJ3DqmPyi00lRotY81EGd81BrHoqwVmMbe3PRr7sF7OPQJYAGvL0AOhs9u6SD8fmRGyVhtF-5D2cOmhsPHpJqdkIwYH0CU5SN_v8_-Qcy2a_e</recordid><startdate>20070424</startdate><enddate>20070424</enddate><creator>Sawada, Tomokazu</creator><creator>Sano, Michio</creator><creator>Omura, Takao</creator><creator>Omura, Kumiko</creator><creator>Hasegawa, Tomohiko</creator><creator>Funahashi, Shinji</creator><creator>Nagano, Akira</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070424</creationdate><title>Spatiotemporal quantification of tumor necrosis factor-alpha and interleukin-10 after crush injury in rat sciatic nerve utilizing immunohistochemistry</title><author>Sawada, Tomokazu ; Sano, Michio ; Omura, Takao ; Omura, Kumiko ; Hasegawa, Tomohiko ; Funahashi, Shinji ; Nagano, Akira</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-dd262c67b2a694526f360fd3a7f37fe6579ae16a87e744df23c33a89d1be5d1e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Axons - immunology</topic><topic>Axons - metabolism</topic><topic>Axons - pathology</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - analysis</topic><topic>Biomarkers - metabolism</topic><topic>Blood–nerve barrier</topic><topic>Cell Count</topic><topic>Cell Size</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Interleukin-10</topic><topic>Interleukin-10 - analysis</topic><topic>Interleukin-10 - immunology</topic><topic>Interleukin-10 - metabolism</topic><topic>Macrophage</topic><topic>Macrophages - immunology</topic><topic>Macrophages - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Schwann Cells - immunology</topic><topic>Schwann Cells - metabolism</topic><topic>Sciatic Nerve - immunology</topic><topic>Sciatic Nerve - metabolism</topic><topic>Sciatic Nerve - physiopathology</topic><topic>Sciatic Neuropathy - immunology</topic><topic>Sciatic Neuropathy - metabolism</topic><topic>Sciatic Neuropathy - physiopathology</topic><topic>Time Factors</topic><topic>Tumor necrosis factor-alpha</topic><topic>Tumor Necrosis Factor-alpha - analysis</topic><topic>Tumor Necrosis Factor-alpha - immunology</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><topic>Up-Regulation - immunology</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>Wallerian degeneration</topic><topic>Wallerian Degeneration - immunology</topic><topic>Wallerian Degeneration - metabolism</topic><topic>Wallerian Degeneration - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sawada, Tomokazu</creatorcontrib><creatorcontrib>Sano, Michio</creatorcontrib><creatorcontrib>Omura, Takao</creatorcontrib><creatorcontrib>Omura, Kumiko</creatorcontrib><creatorcontrib>Hasegawa, Tomohiko</creatorcontrib><creatorcontrib>Funahashi, Shinji</creatorcontrib><creatorcontrib>Nagano, Akira</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sawada, Tomokazu</au><au>Sano, Michio</au><au>Omura, Takao</au><au>Omura, Kumiko</au><au>Hasegawa, Tomohiko</au><au>Funahashi, Shinji</au><au>Nagano, Akira</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spatiotemporal quantification of tumor necrosis factor-alpha and interleukin-10 after crush injury in rat sciatic nerve utilizing immunohistochemistry</atitle><jtitle>Neuroscience letters</jtitle><addtitle>Neurosci Lett</addtitle><date>2007-04-24</date><risdate>2007</risdate><volume>417</volume><issue>1</issue><spage>55</spage><epage>60</epage><pages>55-60</pages><issn>0304-3940</issn><eissn>1872-7972</eissn><coden>NELED5</coden><abstract>The purpose of this study was to investigate quantitatively the longitudinal temporal, spatial changes of the tumor necrosis factor-alpha (TNF) and interleukin-10 (IL-10) immunopositive cells during Wallerian degeneration and the following regeneration after crush injury in rat sciatic nerve using immunohistochemistry and enzyme linked immunosorbent assay (ELISA). The number of TNF-immunopositive cells reached its peak and increased significantly in all the segments distal to the crush site 3 days after injury. On Day 7, TNF-immunopositive cells decreased in all the segments distal to the crush site, and a significant decrease was observed 14 days after injury. From Day 21 to Day 56, there were no significant differences in the numbers of TNF-immunopositive cells. The average size of TNF immunopossitive cells became significantly larger with degeneration. The number of IL-10-immunopositive cells decreases significantly 1 day after crush injury. IL-10-immunopositive cells increased on Day 3, returning to control levels. Seven days after injury, a significant increase in the number of IL-10-immunopositive cells was observed. There was also no significant difference in the number of IL-10-immunopositive cells beyond Day 14 except for a part of distal segments. The number of IL-10-immunopositive cells showed no significant differences in all the segments on Day 56. The protein levels of IL-10 measured by ELISA were similar to the result of immunohistochemistry. These results suggest that the significant change in IL-10 occurred prior to the significant change in TNF and that IL-10 may be the key to the change in TNF.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>17336456</pmid><doi>10.1016/j.neulet.2007.02.028</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Neuroscience letters, 2007-04, Vol.417 (1), p.55-60 |
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| subjects | Animals Axons - immunology Axons - metabolism Axons - pathology Biological and medical sciences Biomarkers - analysis Biomarkers - metabolism Blood–nerve barrier Cell Count Cell Size Enzyme-Linked Immunosorbent Assay Female Fundamental and applied biological sciences. Psychology Immunohistochemistry Interleukin-10 Interleukin-10 - analysis Interleukin-10 - immunology Interleukin-10 - metabolism Macrophage Macrophages - immunology Macrophages - metabolism Rats Rats, Sprague-Dawley Schwann Cells - immunology Schwann Cells - metabolism Sciatic Nerve - immunology Sciatic Nerve - metabolism Sciatic Nerve - physiopathology Sciatic Neuropathy - immunology Sciatic Neuropathy - metabolism Sciatic Neuropathy - physiopathology Time Factors Tumor necrosis factor-alpha Tumor Necrosis Factor-alpha - analysis Tumor Necrosis Factor-alpha - immunology Tumor Necrosis Factor-alpha - metabolism Up-Regulation - immunology Vertebrates: nervous system and sense organs Wallerian degeneration Wallerian Degeneration - immunology Wallerian Degeneration - metabolism Wallerian Degeneration - physiopathology |
| title | Spatiotemporal quantification of tumor necrosis factor-alpha and interleukin-10 after crush injury in rat sciatic nerve utilizing immunohistochemistry |
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