Elucidation of the DNA sequence of Streptococcus uberis adhesion molecule gene ( sua) and detection of sua in strains of Streptococcus uberis isolated from geographically diverse locations
Streptococcus uberis is an important environmental pathogen that causes subclinical and clinical mastitis in lactating and nonlactating cows throughout the world. S. uberis adhesion molecule (SUAM) was identified recently by our laboratory and we hypothesize that SUAM is a potential virulence factor...
Gespeichert in:
| Veröffentlicht in: | Veterinary microbiology 2008-04, Vol.128 (3), p.304-312 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 312 |
|---|---|
| container_issue | 3 |
| container_start_page | 304 |
| container_title | Veterinary microbiology |
| container_volume | 128 |
| creator | Luther, Douglas A. Almeida, Raúl A. Oliver, Stephen P. |
| description | Streptococcus uberis is an important environmental pathogen that causes subclinical and clinical mastitis in lactating and nonlactating cows throughout the world.
S. uberis adhesion molecule (SUAM) was identified recently by our laboratory and we hypothesize that SUAM is a potential virulence factor involved in the pathogenesis of
S. uberis mastitis. The first objective of the present study was to clone and sequence the SUAM gene (
sua) from
S. uberis UT888. The second objective was to determine the prevalence of
sua in strains of
S. uberis isolated from geographically diverse locations. The 20 amino acid N-terminal sequence of purified SUAM was utilized to identify a single open reading frame (ORF) in the
S. uberis O140J (ATCC BAA-854) genome database. Three sets of primers were identified from this sequence for amplification of sub-fragments and the complete gene encoding SUAM. Restriction fragment analysis of the largest polymerase chain reaction (PCR) product confirmed the desired fragment had been amplified. This 2970
bp PCR fragment was cloned into plasmid pCR-XL-TOPO and sequenced. The
S. uberis UT888
sua sequence (NCBI Accession no.
DQ232760) was 99% similar to the
S. uberis O140J database sequence. The three pairs of PCR primers were used in a subsequent experiment to identify
sua in 12 strains of
S. uberis isolated in milk from dairy cows with mastitis in Tennessee (
n
=
6), Colorado (
n
=
1), Washington (
n
=
1), New Zealand (
n
=
1) and from the American Type Culture Collection (
n
=
3). Primer pairs yielded the expected 2970, 2639 and 2362
bp PCR fragments in all strains evaluated. In conclusion, we cloned and sequenced
sua, which codes for the first described
S. uberis adhesin, SUAM.
sua was detected in all strains of
S. uberis evaluated suggesting that it is conserved. |
| doi_str_mv | 10.1016/j.vetmic.2007.10.015 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70359771</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378113507005202</els_id><sourcerecordid>70359771</sourcerecordid><originalsourceid>FETCH-LOGICAL-c445t-5fae4cd9899cd37fa08c43701fe34db8c1e2baa1cb67b47ab53f81abd54551533</originalsourceid><addsrcrecordid>eNqFks2O1DAQhCMEYoeFN0DgCwgOGew4HicXpNWy_EgrOCx7tjp2Z8ajJB7cyUj7bjwcDhngBJwslb4utas6y54KvhZcbN7s10cce2_XBec6SWsu1L1sJSot80KVxf1sxaWuciGkOsseEe0552W94Q-zM1Hxqqh1tcq-X3WT9Q5GHwYWWjbukL37fMEIv004WJy1mzHiYQw2WDsRmxqMnhi4HdI81IcO7dQh2-KA7BWjCV4zGBxzOKL95ZtU5gdGYwQ_0F9dPYUORnSsjaFPjmEb4bDzFrrujjl_xEjIumB_7kuPswctdIRPTu95dvv-6uvlx_z6y4dPlxfXuS1LNeaqBSytq6u6tk7qFnhlS6m5aFGWrqmswKIBELbZ6KbU0CjZVgIap0qlhJLyPHu5-B5iSLHQaHpPFrsOBgwTGc2lqrUW_wVFrRUvCpXAcgFtDEQRW3OIvod4ZwQ3c71mb5Z6zVzvrKZ609izk__U9Oj-DJ36TMCLEwCUQmsjDNbTb67gyU3yTeKeL1wLwcA2RW9ubwouJOfpfjZ6_vPbhcAU7NFjNGT9fBHOx1SrccH_e9cfktrTAg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19750225</pqid></control><display><type>article</type><title>Elucidation of the DNA sequence of Streptococcus uberis adhesion molecule gene ( sua) and detection of sua in strains of Streptococcus uberis isolated from geographically diverse locations</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Luther, Douglas A. ; Almeida, Raúl A. ; Oliver, Stephen P.</creator><creatorcontrib>Luther, Douglas A. ; Almeida, Raúl A. ; Oliver, Stephen P.</creatorcontrib><description>Streptococcus uberis is an important environmental pathogen that causes subclinical and clinical mastitis in lactating and nonlactating cows throughout the world.
S. uberis adhesion molecule (SUAM) was identified recently by our laboratory and we hypothesize that SUAM is a potential virulence factor involved in the pathogenesis of
S. uberis mastitis. The first objective of the present study was to clone and sequence the SUAM gene (
sua) from
S. uberis UT888. The second objective was to determine the prevalence of
sua in strains of
S. uberis isolated from geographically diverse locations. The 20 amino acid N-terminal sequence of purified SUAM was utilized to identify a single open reading frame (ORF) in the
S. uberis O140J (ATCC BAA-854) genome database. Three sets of primers were identified from this sequence for amplification of sub-fragments and the complete gene encoding SUAM. Restriction fragment analysis of the largest polymerase chain reaction (PCR) product confirmed the desired fragment had been amplified. This 2970
bp PCR fragment was cloned into plasmid pCR-XL-TOPO and sequenced. The
S. uberis UT888
sua sequence (NCBI Accession no.
DQ232760) was 99% similar to the
S. uberis O140J database sequence. The three pairs of PCR primers were used in a subsequent experiment to identify
sua in 12 strains of
S. uberis isolated in milk from dairy cows with mastitis in Tennessee (
n
=
6), Colorado (
n
=
1), Washington (
n
=
1), New Zealand (
n
=
1) and from the American Type Culture Collection (
n
=
3). Primer pairs yielded the expected 2970, 2639 and 2362
bp PCR fragments in all strains evaluated. In conclusion, we cloned and sequenced
sua, which codes for the first described
S. uberis adhesin, SUAM.
sua was detected in all strains of
S. uberis evaluated suggesting that it is conserved.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2007.10.015</identifier><identifier>PMID: 18082978</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>adhesins ; Adhesins, Bacterial - genetics ; Amino Acid Sequence ; animal pathogenic bacteria ; Animals ; bacterial adhesion ; Bacterial Adhesion - genetics ; Bacterial Adhesion - physiology ; Bacteriology ; Base Sequence ; Biological and medical sciences ; bovine mastitis ; Cattle ; Cloning, Molecular ; DNA ; DNA Primers ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Amplification ; genes ; geographical variation ; isolation ; Mastitis ; Mastitis, Bovine - microbiology ; microbial detection ; microbial genetics ; Microbiology ; Milk - microbiology ; Miscellaneous ; Molecular Sequence Data ; Molecular Weight ; nucleotide sequences ; Open Reading Frames ; Plasmids ; Polymorphism, Restriction Fragment Length ; strains ; Streptococcus - genetics ; Streptococcus - pathogenicity ; Streptococcus uberis ; Streptococcus uberis adhesion molecule ; sua</subject><ispartof>Veterinary microbiology, 2008-04, Vol.128 (3), p.304-312</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-5fae4cd9899cd37fa08c43701fe34db8c1e2baa1cb67b47ab53f81abd54551533</citedby><cites>FETCH-LOGICAL-c445t-5fae4cd9899cd37fa08c43701fe34db8c1e2baa1cb67b47ab53f81abd54551533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113507005202$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20200306$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18082978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Luther, Douglas A.</creatorcontrib><creatorcontrib>Almeida, Raúl A.</creatorcontrib><creatorcontrib>Oliver, Stephen P.</creatorcontrib><title>Elucidation of the DNA sequence of Streptococcus uberis adhesion molecule gene ( sua) and detection of sua in strains of Streptococcus uberis isolated from geographically diverse locations</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Streptococcus uberis is an important environmental pathogen that causes subclinical and clinical mastitis in lactating and nonlactating cows throughout the world.
S. uberis adhesion molecule (SUAM) was identified recently by our laboratory and we hypothesize that SUAM is a potential virulence factor involved in the pathogenesis of
S. uberis mastitis. The first objective of the present study was to clone and sequence the SUAM gene (
sua) from
S. uberis UT888. The second objective was to determine the prevalence of
sua in strains of
S. uberis isolated from geographically diverse locations. The 20 amino acid N-terminal sequence of purified SUAM was utilized to identify a single open reading frame (ORF) in the
S. uberis O140J (ATCC BAA-854) genome database. Three sets of primers were identified from this sequence for amplification of sub-fragments and the complete gene encoding SUAM. Restriction fragment analysis of the largest polymerase chain reaction (PCR) product confirmed the desired fragment had been amplified. This 2970
bp PCR fragment was cloned into plasmid pCR-XL-TOPO and sequenced. The
S. uberis UT888
sua sequence (NCBI Accession no.
DQ232760) was 99% similar to the
S. uberis O140J database sequence. The three pairs of PCR primers were used in a subsequent experiment to identify
sua in 12 strains of
S. uberis isolated in milk from dairy cows with mastitis in Tennessee (
n
=
6), Colorado (
n
=
1), Washington (
n
=
1), New Zealand (
n
=
1) and from the American Type Culture Collection (
n
=
3). Primer pairs yielded the expected 2970, 2639 and 2362
bp PCR fragments in all strains evaluated. In conclusion, we cloned and sequenced
sua, which codes for the first described
S. uberis adhesin, SUAM.
sua was detected in all strains of
S. uberis evaluated suggesting that it is conserved.</description><subject>adhesins</subject><subject>Adhesins, Bacterial - genetics</subject><subject>Amino Acid Sequence</subject><subject>animal pathogenic bacteria</subject><subject>Animals</subject><subject>bacterial adhesion</subject><subject>Bacterial Adhesion - genetics</subject><subject>Bacterial Adhesion - physiology</subject><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>bovine mastitis</subject><subject>Cattle</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Amplification</subject><subject>genes</subject><subject>geographical variation</subject><subject>isolation</subject><subject>Mastitis</subject><subject>Mastitis, Bovine - microbiology</subject><subject>microbial detection</subject><subject>microbial genetics</subject><subject>Microbiology</subject><subject>Milk - microbiology</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Molecular Weight</subject><subject>nucleotide sequences</subject><subject>Open Reading Frames</subject><subject>Plasmids</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>strains</subject><subject>Streptococcus - genetics</subject><subject>Streptococcus - pathogenicity</subject><subject>Streptococcus uberis</subject><subject>Streptococcus uberis adhesion molecule</subject><subject>sua</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks2O1DAQhCMEYoeFN0DgCwgOGew4HicXpNWy_EgrOCx7tjp2Z8ajJB7cyUj7bjwcDhngBJwslb4utas6y54KvhZcbN7s10cce2_XBec6SWsu1L1sJSot80KVxf1sxaWuciGkOsseEe0552W94Q-zM1Hxqqh1tcq-X3WT9Q5GHwYWWjbukL37fMEIv004WJy1mzHiYQw2WDsRmxqMnhi4HdI81IcO7dQh2-KA7BWjCV4zGBxzOKL95ZtU5gdGYwQ_0F9dPYUORnSsjaFPjmEb4bDzFrrujjl_xEjIumB_7kuPswctdIRPTu95dvv-6uvlx_z6y4dPlxfXuS1LNeaqBSytq6u6tk7qFnhlS6m5aFGWrqmswKIBELbZ6KbU0CjZVgIap0qlhJLyPHu5-B5iSLHQaHpPFrsOBgwTGc2lqrUW_wVFrRUvCpXAcgFtDEQRW3OIvod4ZwQ3c71mb5Z6zVzvrKZ609izk__U9Oj-DJ36TMCLEwCUQmsjDNbTb67gyU3yTeKeL1wLwcA2RW9ubwouJOfpfjZ6_vPbhcAU7NFjNGT9fBHOx1SrccH_e9cfktrTAg</recordid><startdate>20080430</startdate><enddate>20080430</enddate><creator>Luther, Douglas A.</creator><creator>Almeida, Raúl A.</creator><creator>Oliver, Stephen P.</creator><general>Elsevier B.V</general><general>Amsterdam; New York: Elsevier</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20080430</creationdate><title>Elucidation of the DNA sequence of Streptococcus uberis adhesion molecule gene ( sua) and detection of sua in strains of Streptococcus uberis isolated from geographically diverse locations</title><author>Luther, Douglas A. ; Almeida, Raúl A. ; Oliver, Stephen P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c445t-5fae4cd9899cd37fa08c43701fe34db8c1e2baa1cb67b47ab53f81abd54551533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>adhesins</topic><topic>Adhesins, Bacterial - genetics</topic><topic>Amino Acid Sequence</topic><topic>animal pathogenic bacteria</topic><topic>Animals</topic><topic>bacterial adhesion</topic><topic>Bacterial Adhesion - genetics</topic><topic>Bacterial Adhesion - physiology</topic><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>bovine mastitis</topic><topic>Cattle</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Amplification</topic><topic>genes</topic><topic>geographical variation</topic><topic>isolation</topic><topic>Mastitis</topic><topic>Mastitis, Bovine - microbiology</topic><topic>microbial detection</topic><topic>microbial genetics</topic><topic>Microbiology</topic><topic>Milk - microbiology</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Molecular Weight</topic><topic>nucleotide sequences</topic><topic>Open Reading Frames</topic><topic>Plasmids</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>strains</topic><topic>Streptococcus - genetics</topic><topic>Streptococcus - pathogenicity</topic><topic>Streptococcus uberis</topic><topic>Streptococcus uberis adhesion molecule</topic><topic>sua</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Luther, Douglas A.</creatorcontrib><creatorcontrib>Almeida, Raúl A.</creatorcontrib><creatorcontrib>Oliver, Stephen P.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Luther, Douglas A.</au><au>Almeida, Raúl A.</au><au>Oliver, Stephen P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Elucidation of the DNA sequence of Streptococcus uberis adhesion molecule gene ( sua) and detection of sua in strains of Streptococcus uberis isolated from geographically diverse locations</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2008-04-30</date><risdate>2008</risdate><volume>128</volume><issue>3</issue><spage>304</spage><epage>312</epage><pages>304-312</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Streptococcus uberis is an important environmental pathogen that causes subclinical and clinical mastitis in lactating and nonlactating cows throughout the world.
S. uberis adhesion molecule (SUAM) was identified recently by our laboratory and we hypothesize that SUAM is a potential virulence factor involved in the pathogenesis of
S. uberis mastitis. The first objective of the present study was to clone and sequence the SUAM gene (
sua) from
S. uberis UT888. The second objective was to determine the prevalence of
sua in strains of
S. uberis isolated from geographically diverse locations. The 20 amino acid N-terminal sequence of purified SUAM was utilized to identify a single open reading frame (ORF) in the
S. uberis O140J (ATCC BAA-854) genome database. Three sets of primers were identified from this sequence for amplification of sub-fragments and the complete gene encoding SUAM. Restriction fragment analysis of the largest polymerase chain reaction (PCR) product confirmed the desired fragment had been amplified. This 2970
bp PCR fragment was cloned into plasmid pCR-XL-TOPO and sequenced. The
S. uberis UT888
sua sequence (NCBI Accession no.
DQ232760) was 99% similar to the
S. uberis O140J database sequence. The three pairs of PCR primers were used in a subsequent experiment to identify
sua in 12 strains of
S. uberis isolated in milk from dairy cows with mastitis in Tennessee (
n
=
6), Colorado (
n
=
1), Washington (
n
=
1), New Zealand (
n
=
1) and from the American Type Culture Collection (
n
=
3). Primer pairs yielded the expected 2970, 2639 and 2362
bp PCR fragments in all strains evaluated. In conclusion, we cloned and sequenced
sua, which codes for the first described
S. uberis adhesin, SUAM.
sua was detected in all strains of
S. uberis evaluated suggesting that it is conserved.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>18082978</pmid><doi>10.1016/j.vetmic.2007.10.015</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 2008-04, Vol.128 (3), p.304-312 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70359771 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | adhesins Adhesins, Bacterial - genetics Amino Acid Sequence animal pathogenic bacteria Animals bacterial adhesion Bacterial Adhesion - genetics Bacterial Adhesion - physiology Bacteriology Base Sequence Biological and medical sciences bovine mastitis Cattle Cloning, Molecular DNA DNA Primers Female Fundamental and applied biological sciences. Psychology Gene Amplification genes geographical variation isolation Mastitis Mastitis, Bovine - microbiology microbial detection microbial genetics Microbiology Milk - microbiology Miscellaneous Molecular Sequence Data Molecular Weight nucleotide sequences Open Reading Frames Plasmids Polymorphism, Restriction Fragment Length strains Streptococcus - genetics Streptococcus - pathogenicity Streptococcus uberis Streptococcus uberis adhesion molecule sua |
| title | Elucidation of the DNA sequence of Streptococcus uberis adhesion molecule gene ( sua) and detection of sua in strains of Streptococcus uberis isolated from geographically diverse locations |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T03%3A07%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Elucidation%20of%20the%20DNA%20sequence%20of%20Streptococcus%20uberis%20adhesion%20molecule%20gene%20(%20sua)%20and%20detection%20of%20sua%20in%20strains%20of%20Streptococcus%20uberis%20isolated%20from%20geographically%20diverse%20locations&rft.jtitle=Veterinary%20microbiology&rft.au=Luther,%20Douglas%20A.&rft.date=2008-04-30&rft.volume=128&rft.issue=3&rft.spage=304&rft.epage=312&rft.pages=304-312&rft.issn=0378-1135&rft.eissn=1873-2542&rft.coden=VMICDQ&rft_id=info:doi/10.1016/j.vetmic.2007.10.015&rft_dat=%3Cproquest_cross%3E70359771%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19750225&rft_id=info:pmid/18082978&rft_els_id=S0378113507005202&rfr_iscdi=true |