Microvascular and systemic effects following top load administration of saturated carbon monoxide-saline solution
OBJECTIVE:To determine how top loads with different doses of carbon monoxide (CO)-saturated saline solutions (CO-saline) affect microvascular and systemic hemodynamics and to delineate the corresponding biochemical mechanisms. DESIGN:Prospective study. SETTING:University research laboratory. SUBJECT...
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| Veröffentlicht in: | Critical care medicine 2007-04, Vol.35 (4), p.1123-1132 |
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| creator | Hangai-Hoger, Nanae Tsai, Amy G Cabrales, Pedro Suematsu, Makoto Intaglietta, Marcos |
| description | OBJECTIVE:To determine how top loads with different doses of carbon monoxide (CO)-saturated saline solutions (CO-saline) affect microvascular and systemic hemodynamics and to delineate the corresponding biochemical mechanisms.
DESIGN:Prospective study.
SETTING:University research laboratory.
SUBJECTS:Male Golden Syrian hamsters.
INTERVENTIONS:Hamsters implemented with a dorsal window chamber were given different volumes (characterized as percent of blood volume, BV) by intravenous injection of CO-saturated saline.
MEASUREMENTS AND MAIN RESULTS:Hamsters were observed until 90 mins after infusion of CO-saline solution. In the 20% BV CO-saline infusion group, observation was extended until 180 mins. Systemic variables measured included mean arterial pressure, heart rate, systemic arterial blood gases, and cardiac output and index. Microvascular hemodynamic measurements included vessel diameter, red blood cell velocity, and functional capillary density. Cyclic guanosine monophosphate (cGMP) content in the chamber tissue was measured by enzyme immunoassay. 10% BV of CO-saline increased flow maximally in the microcirculation at 30 mins after infusion (207% in arterioles and 238% in venules, p < .05 vs. baseline). Functional capillary density was significantly increased in both 10% and 15% groups (p < .05 vs. baseline), and cardiac index increased 130% (p < .05 vs. baseline) at 10 mins after 10% CO-saline infusion. There were no changes of microhemodynamic variables and functional capillary density with 2.5%, 5%, and 20% CO-saline infusion during the observation period. Microvascular hemodynamic changes by 10% CO-saline infusion were inhibited completely by L-NAME pretreatment and partially by 1H-[1,2,4]oxadiazole[4,3-a]quinoxqalin-1-one pretreatment. cGMP content in skin fold tissues was related to changes of vessel diameter.
CONCLUSIONS:Intravenous injection of CO-saturated saline caused vasodilation and improved microvascular hemodynamics in the hamster window chamber model in a dose-dependent manner. These changes were related to increased cardiac output and local cGMP content. These results support the possible use of CO-saturated solutions as a vasodilator in critical conditions. |
| doi_str_mv | 10.1097/01.CCM.0000259533.84180.C7 |
| format | Article |
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DESIGN:Prospective study.
SETTING:University research laboratory.
SUBJECTS:Male Golden Syrian hamsters.
INTERVENTIONS:Hamsters implemented with a dorsal window chamber were given different volumes (characterized as percent of blood volume, BV) by intravenous injection of CO-saturated saline.
MEASUREMENTS AND MAIN RESULTS:Hamsters were observed until 90 mins after infusion of CO-saline solution. In the 20% BV CO-saline infusion group, observation was extended until 180 mins. Systemic variables measured included mean arterial pressure, heart rate, systemic arterial blood gases, and cardiac output and index. Microvascular hemodynamic measurements included vessel diameter, red blood cell velocity, and functional capillary density. Cyclic guanosine monophosphate (cGMP) content in the chamber tissue was measured by enzyme immunoassay. 10% BV of CO-saline increased flow maximally in the microcirculation at 30 mins after infusion (207% in arterioles and 238% in venules, p < .05 vs. baseline). Functional capillary density was significantly increased in both 10% and 15% groups (p < .05 vs. baseline), and cardiac index increased 130% (p < .05 vs. baseline) at 10 mins after 10% CO-saline infusion. There were no changes of microhemodynamic variables and functional capillary density with 2.5%, 5%, and 20% CO-saline infusion during the observation period. Microvascular hemodynamic changes by 10% CO-saline infusion were inhibited completely by L-NAME pretreatment and partially by 1H-[1,2,4]oxadiazole[4,3-a]quinoxqalin-1-one pretreatment. cGMP content in skin fold tissues was related to changes of vessel diameter.
CONCLUSIONS:Intravenous injection of CO-saturated saline caused vasodilation and improved microvascular hemodynamics in the hamster window chamber model in a dose-dependent manner. These changes were related to increased cardiac output and local cGMP content. These results support the possible use of CO-saturated solutions as a vasodilator in critical conditions.</description><identifier>ISSN: 0090-3493</identifier><identifier>EISSN: 1530-0293</identifier><identifier>DOI: 10.1097/01.CCM.0000259533.84180.C7</identifier><identifier>PMID: 17334240</identifier><identifier>CODEN: CCMDC7</identifier><language>eng</language><publisher>Hagerstown, MD: by the Society of Critical Care Medicine and Lippincott Williams & Wilkins</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Biological and medical sciences ; Blood Gas Analysis ; Blood Pressure - drug effects ; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis ; Blood. Blood coagulation. Reticuloendothelial system ; Carbon Monoxide - administration & dosage ; Carbon Monoxide - pharmacology ; Carboxyhemoglobin - analysis ; Cardiac Output - drug effects ; Cricetinae ; Cyclic GMP - biosynthesis ; Dose-Response Relationship, Drug ; Heart Rate - drug effects ; Intensive care medicine ; Male ; Medical sciences ; Mesocricetus ; Microcirculation - drug effects ; NG-Nitroarginine Methyl Ester - pharmacology ; Nitric Oxide Synthase - antagonists & inhibitors ; Pharmacology. Drug treatments ; Skin - blood supply ; Sodium Chloride - administration & dosage ; Sodium Chloride - pharmacology ; Transfusions. Complications. Transfusion reactions. Cell and gene therapy ; Vasodilation - drug effects</subject><ispartof>Critical care medicine, 2007-04, Vol.35 (4), p.1123-1132</ispartof><rights>2007 by the Society of Critical Care Medicine and Lippincott Williams & Wilkins</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4588-d009cf3b18e7bfaf1cbde884e20d652b8680b37ef9c7abd70b9d8c7a432b6b663</citedby><cites>FETCH-LOGICAL-c4588-d009cf3b18e7bfaf1cbde884e20d652b8680b37ef9c7abd70b9d8c7a432b6b663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18621398$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17334240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hangai-Hoger, Nanae</creatorcontrib><creatorcontrib>Tsai, Amy G</creatorcontrib><creatorcontrib>Cabrales, Pedro</creatorcontrib><creatorcontrib>Suematsu, Makoto</creatorcontrib><creatorcontrib>Intaglietta, Marcos</creatorcontrib><title>Microvascular and systemic effects following top load administration of saturated carbon monoxide-saline solution</title><title>Critical care medicine</title><addtitle>Crit Care Med</addtitle><description>OBJECTIVE:To determine how top loads with different doses of carbon monoxide (CO)-saturated saline solutions (CO-saline) affect microvascular and systemic hemodynamics and to delineate the corresponding biochemical mechanisms.
DESIGN:Prospective study.
SETTING:University research laboratory.
SUBJECTS:Male Golden Syrian hamsters.
INTERVENTIONS:Hamsters implemented with a dorsal window chamber were given different volumes (characterized as percent of blood volume, BV) by intravenous injection of CO-saturated saline.
MEASUREMENTS AND MAIN RESULTS:Hamsters were observed until 90 mins after infusion of CO-saline solution. In the 20% BV CO-saline infusion group, observation was extended until 180 mins. Systemic variables measured included mean arterial pressure, heart rate, systemic arterial blood gases, and cardiac output and index. Microvascular hemodynamic measurements included vessel diameter, red blood cell velocity, and functional capillary density. Cyclic guanosine monophosphate (cGMP) content in the chamber tissue was measured by enzyme immunoassay. 10% BV of CO-saline increased flow maximally in the microcirculation at 30 mins after infusion (207% in arterioles and 238% in venules, p < .05 vs. baseline). Functional capillary density was significantly increased in both 10% and 15% groups (p < .05 vs. baseline), and cardiac index increased 130% (p < .05 vs. baseline) at 10 mins after 10% CO-saline infusion. There were no changes of microhemodynamic variables and functional capillary density with 2.5%, 5%, and 20% CO-saline infusion during the observation period. Microvascular hemodynamic changes by 10% CO-saline infusion were inhibited completely by L-NAME pretreatment and partially by 1H-[1,2,4]oxadiazole[4,3-a]quinoxqalin-1-one pretreatment. cGMP content in skin fold tissues was related to changes of vessel diameter.
CONCLUSIONS:Intravenous injection of CO-saturated saline caused vasodilation and improved microvascular hemodynamics in the hamster window chamber model in a dose-dependent manner. These changes were related to increased cardiac output and local cGMP content. These results support the possible use of CO-saturated solutions as a vasodilator in critical conditions.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Gas Analysis</subject><subject>Blood Pressure - drug effects</subject><subject>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</subject><subject>Blood. Blood coagulation. Reticuloendothelial system</subject><subject>Carbon Monoxide - administration & dosage</subject><subject>Carbon Monoxide - pharmacology</subject><subject>Carboxyhemoglobin - analysis</subject><subject>Cardiac Output - drug effects</subject><subject>Cricetinae</subject><subject>Cyclic GMP - biosynthesis</subject><subject>Dose-Response Relationship, Drug</subject><subject>Heart Rate - drug effects</subject><subject>Intensive care medicine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mesocricetus</subject><subject>Microcirculation - drug effects</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Pharmacology. Drug treatments</subject><subject>Skin - blood supply</subject><subject>Sodium Chloride - administration & dosage</subject><subject>Sodium Chloride - pharmacology</subject><subject>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</subject><subject>Vasodilation - drug effects</subject><issn>0090-3493</issn><issn>1530-0293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkF1vFCEUhonR2LX6Fwwx0bsZDwMzMN6ZidombbzRa8KnRZlhCzOu_ffS7iZLQuCQ55yXPAi9I9ASGPlHIO003bZQV9ePPaWtYERAO_FnaEd6Cg10I32OdgAjNJSN9AK9KuU3AGE9py_RBeGUso7BDt3fBpPTX1XMFlXGarG4PJTVzcFg570za8E-xZgOYfmF17THMSmLlZ3DEsqa1RrSgpPHRa1brZzFRmVd3-a0pH_BuqaoGBaHS4rbI_wavfAqFvfmdF6in1-__Jiumpvv366nzzeNYb0Qja2fN55qIhzXXnlitHVCMNeBHfpOi0GAptz50XClLQc9WlGvjHZ60MNAL9GH49x9TvebK6ucQzEuRrW4tBXJgdacjlbw0xGsIkrJzst9DrPKD5KAfBQugcgqXJ6FyyfhcuK1-e0pZdOzs-fWk-EKvD8B1bGKPqvFhHLmxNAROorKsSN3SHF1ufyJ28FleedUXO-eomnHhqYD4MBq1dRNBP0Po4ucbg</recordid><startdate>200704</startdate><enddate>200704</enddate><creator>Hangai-Hoger, Nanae</creator><creator>Tsai, Amy G</creator><creator>Cabrales, Pedro</creator><creator>Suematsu, Makoto</creator><creator>Intaglietta, Marcos</creator><general>by the Society of Critical Care Medicine and Lippincott Williams & Wilkins</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200704</creationdate><title>Microvascular and systemic effects following top load administration of saturated carbon monoxide-saline solution</title><author>Hangai-Hoger, Nanae ; Tsai, Amy G ; Cabrales, Pedro ; Suematsu, Makoto ; Intaglietta, Marcos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4588-d009cf3b18e7bfaf1cbde884e20d652b8680b37ef9c7abd70b9d8c7a432b6b663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Gas Analysis</topic><topic>Blood Pressure - drug effects</topic><topic>Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis</topic><topic>Blood. Blood coagulation. Reticuloendothelial system</topic><topic>Carbon Monoxide - administration & dosage</topic><topic>Carbon Monoxide - pharmacology</topic><topic>Carboxyhemoglobin - analysis</topic><topic>Cardiac Output - drug effects</topic><topic>Cricetinae</topic><topic>Cyclic GMP - biosynthesis</topic><topic>Dose-Response Relationship, Drug</topic><topic>Heart Rate - drug effects</topic><topic>Intensive care medicine</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mesocricetus</topic><topic>Microcirculation - drug effects</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Pharmacology. Drug treatments</topic><topic>Skin - blood supply</topic><topic>Sodium Chloride - administration & dosage</topic><topic>Sodium Chloride - pharmacology</topic><topic>Transfusions. Complications. Transfusion reactions. Cell and gene therapy</topic><topic>Vasodilation - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hangai-Hoger, Nanae</creatorcontrib><creatorcontrib>Tsai, Amy G</creatorcontrib><creatorcontrib>Cabrales, Pedro</creatorcontrib><creatorcontrib>Suematsu, Makoto</creatorcontrib><creatorcontrib>Intaglietta, Marcos</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Critical care medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hangai-Hoger, Nanae</au><au>Tsai, Amy G</au><au>Cabrales, Pedro</au><au>Suematsu, Makoto</au><au>Intaglietta, Marcos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microvascular and systemic effects following top load administration of saturated carbon monoxide-saline solution</atitle><jtitle>Critical care medicine</jtitle><addtitle>Crit Care Med</addtitle><date>2007-04</date><risdate>2007</risdate><volume>35</volume><issue>4</issue><spage>1123</spage><epage>1132</epage><pages>1123-1132</pages><issn>0090-3493</issn><eissn>1530-0293</eissn><coden>CCMDC7</coden><abstract>OBJECTIVE:To determine how top loads with different doses of carbon monoxide (CO)-saturated saline solutions (CO-saline) affect microvascular and systemic hemodynamics and to delineate the corresponding biochemical mechanisms.
DESIGN:Prospective study.
SETTING:University research laboratory.
SUBJECTS:Male Golden Syrian hamsters.
INTERVENTIONS:Hamsters implemented with a dorsal window chamber were given different volumes (characterized as percent of blood volume, BV) by intravenous injection of CO-saturated saline.
MEASUREMENTS AND MAIN RESULTS:Hamsters were observed until 90 mins after infusion of CO-saline solution. In the 20% BV CO-saline infusion group, observation was extended until 180 mins. Systemic variables measured included mean arterial pressure, heart rate, systemic arterial blood gases, and cardiac output and index. Microvascular hemodynamic measurements included vessel diameter, red blood cell velocity, and functional capillary density. Cyclic guanosine monophosphate (cGMP) content in the chamber tissue was measured by enzyme immunoassay. 10% BV of CO-saline increased flow maximally in the microcirculation at 30 mins after infusion (207% in arterioles and 238% in venules, p < .05 vs. baseline). Functional capillary density was significantly increased in both 10% and 15% groups (p < .05 vs. baseline), and cardiac index increased 130% (p < .05 vs. baseline) at 10 mins after 10% CO-saline infusion. There were no changes of microhemodynamic variables and functional capillary density with 2.5%, 5%, and 20% CO-saline infusion during the observation period. Microvascular hemodynamic changes by 10% CO-saline infusion were inhibited completely by L-NAME pretreatment and partially by 1H-[1,2,4]oxadiazole[4,3-a]quinoxqalin-1-one pretreatment. cGMP content in skin fold tissues was related to changes of vessel diameter.
CONCLUSIONS:Intravenous injection of CO-saturated saline caused vasodilation and improved microvascular hemodynamics in the hamster window chamber model in a dose-dependent manner. These changes were related to increased cardiac output and local cGMP content. These results support the possible use of CO-saturated solutions as a vasodilator in critical conditions.</abstract><cop>Hagerstown, MD</cop><pub>by the Society of Critical Care Medicine and Lippincott Williams & Wilkins</pub><pmid>17334240</pmid><doi>10.1097/01.CCM.0000259533.84180.C7</doi><tpages>10</tpages></addata></record> |
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| ispartof | Critical care medicine, 2007-04, Vol.35 (4), p.1123-1132 |
| issn | 0090-3493 1530-0293 |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Biological and medical sciences Blood Gas Analysis Blood Pressure - drug effects Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis Blood. Blood coagulation. Reticuloendothelial system Carbon Monoxide - administration & dosage Carbon Monoxide - pharmacology Carboxyhemoglobin - analysis Cardiac Output - drug effects Cricetinae Cyclic GMP - biosynthesis Dose-Response Relationship, Drug Heart Rate - drug effects Intensive care medicine Male Medical sciences Mesocricetus Microcirculation - drug effects NG-Nitroarginine Methyl Ester - pharmacology Nitric Oxide Synthase - antagonists & inhibitors Pharmacology. Drug treatments Skin - blood supply Sodium Chloride - administration & dosage Sodium Chloride - pharmacology Transfusions. Complications. Transfusion reactions. Cell and gene therapy Vasodilation - drug effects |
| title | Microvascular and systemic effects following top load administration of saturated carbon monoxide-saline solution |
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