Quantification of protein based on single-molecule counting by total internal reflection fluorescence microscopy with adsorption equilibrium

We developed a sensitive single-molecule imaging method for quantification of protein by total internal reflection fluorescence microscopy with adsorption equilibrium. In this method, the adsorption equilibrium of protein was achieved between solution and glass substrate. Then, fluorescence images o...

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Veröffentlicht in:	Analytica chimica acta 2007-05, Vol.590 (1), p.104-109
Hauptverfasser:	Wang, Lei, Xu, Guang, Shi, Zhikun, Jiang, Wei, Jin, Wenrui
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Analytical chemistry Chemistry Exact sciences and technology Immunoglobulin Heavy Chains - analysis Microscopy, Fluorescence - methods Protein Proteins - analysis Single-molecule detection Spectrometric and optical methods Total internal reflection fluorescence microscopy
Online-Zugang:	Volltext
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