Comparative analysis of serum proteomes to discover biomarkers for ossification of the posterior longitudinal ligament
Serum proteomes from normal subjects and the patients with ossification of the posterior longitudinal ligament (OPLL) were analyzed by using proteomics. To identify novel serologic biomarkers for diagnosing OPLL. OPLL can compress the spinal cord, and special planning is required for surgeries that...
Gespeichert in:
| Veröffentlicht in: | Spine (Philadelphia, Pa. 1976) Pa. 1976), 2007-04, Vol.32 (7), p.728-734 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 734 |
|---|---|
| container_issue | 7 |
| container_start_page | 728 |
| container_title | Spine (Philadelphia, Pa. 1976) |
| container_volume | 32 |
| creator | EUN, Jong-Pil MA, Tian-Ze LEE, Woo-Jong KIM, Min-Gul YOO, Min-Jeong KOH, Eun-Jung CHOI, Ha-Young KWAK, Yong-Geun |
| description | Serum proteomes from normal subjects and the patients with ossification of the posterior longitudinal ligament (OPLL) were analyzed by using proteomics.
To identify novel serologic biomarkers for diagnosing OPLL.
OPLL can compress the spinal cord, and special planning is required for surgeries that are done from the front of the cervical spine. However, the definitive serologic biomarkers for OPLL are still unclear.
The 2-dimensional electrophoresis patterns of sera from OPLL patients and normal subjects were compared. The differentially expressed spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization quadruple time-of-flight mass spectrometry.
Nine spots that were differentially expressed in the sera of OPLL patients were found and were identified. PRO2675, human serum albumin in a complex with myristic acid and tri-iodobenzoic acid, an unknown protein, chain B of the crystal structure of deoxy-human hemoglobin beta6, pro-apolipoprotein, ALB protein, retinol binding protein and chain A of human serum albumin mutant R218h complexed with thyroxine (3,3',5,5', tetraiodo-L-thyronine), were up-regulated in the sera of OPLL patients, whereas alpha1-microglobulin/bikunin precursor was down-regulated.
These proteins could be used as diagnostic biomarkers of OPLL. |
| doi_str_mv | 10.1097/01.brs.0000259070.66805.93 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70355757</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70355757</sourcerecordid><originalsourceid>FETCH-LOGICAL-c413t-aa34993c4649ad1e17dd48aab4746712f25c906e9ee2cdb2f66c9de8b7094d1f3</originalsourceid><addsrcrecordid>eNpFkE9vFCEchomxsdvqVzDERG8zheHf4M1sbGvSxIueCQM_KjozrMBs0m8vtZssFw4870veB6EPlPSUaHVDaD_l0pN2BqGJIr2UIxG9Zq_Qjoph7CgV-jXaESaHbuBMXqKrUn43XjKq36BLqjjlmogdOu7TcrDZ1ngEbFc7P5VYcAq4QN4WfMipQlqg4Jqwj8WlI2Q8xbTY_AdywSFlnEqJIbrWkdbnaP0F-JBKhRzb65zWx1g3H1s5nuOjXWCtb9FFsHOBd6f7Gv28_fpjf989fL_7tv_y0DlOWe2sZVxr5rjk2noKVHnPR2snrrhUdAiDcJpI0ACD89MQpHTawzgpormngV2jTy-9bcjfDUo1SxsB82xXSFsxijAhlFAN_PwCutzmZAjmkGMb-WQoMc_WDaGmWTdn6-a_daNZC78__bJNC_hz9KS5AR9PgC3OziHb1cVy5kYpuBw1-wd9bI93</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70355757</pqid></control><display><type>article</type><title>Comparative analysis of serum proteomes to discover biomarkers for ossification of the posterior longitudinal ligament</title><source>MEDLINE</source><source>Journals@Ovid Complete</source><creator>EUN, Jong-Pil ; MA, Tian-Ze ; LEE, Woo-Jong ; KIM, Min-Gul ; YOO, Min-Jeong ; KOH, Eun-Jung ; CHOI, Ha-Young ; KWAK, Yong-Geun</creator><creatorcontrib>EUN, Jong-Pil ; MA, Tian-Ze ; LEE, Woo-Jong ; KIM, Min-Gul ; YOO, Min-Jeong ; KOH, Eun-Jung ; CHOI, Ha-Young ; KWAK, Yong-Geun</creatorcontrib><description>Serum proteomes from normal subjects and the patients with ossification of the posterior longitudinal ligament (OPLL) were analyzed by using proteomics.
To identify novel serologic biomarkers for diagnosing OPLL.
OPLL can compress the spinal cord, and special planning is required for surgeries that are done from the front of the cervical spine. However, the definitive serologic biomarkers for OPLL are still unclear.
The 2-dimensional electrophoresis patterns of sera from OPLL patients and normal subjects were compared. The differentially expressed spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization quadruple time-of-flight mass spectrometry.
Nine spots that were differentially expressed in the sera of OPLL patients were found and were identified. PRO2675, human serum albumin in a complex with myristic acid and tri-iodobenzoic acid, an unknown protein, chain B of the crystal structure of deoxy-human hemoglobin beta6, pro-apolipoprotein, ALB protein, retinol binding protein and chain A of human serum albumin mutant R218h complexed with thyroxine (3,3',5,5', tetraiodo-L-thyronine), were up-regulated in the sera of OPLL patients, whereas alpha1-microglobulin/bikunin precursor was down-regulated.
These proteins could be used as diagnostic biomarkers of OPLL.</description><identifier>ISSN: 0362-2436</identifier><identifier>EISSN: 1528-1159</identifier><identifier>DOI: 10.1097/01.brs.0000259070.66805.93</identifier><identifier>PMID: 17414905</identifier><identifier>CODEN: SPINDD</identifier><language>eng</language><publisher>Philadelphia, PA: Lippincott</publisher><subject>Adult ; Apolipoproteins - blood ; Apolipoproteins - genetics ; Biological and medical sciences ; Biomarkers - metabolism ; Case-Control Studies ; Cerebrospinal fluid. Meninges. Spinal cord ; Electrophoresis, Gel, Two-Dimensional ; Female ; Glycoproteins - blood ; Glycoproteins - genetics ; Hemoglobins - genetics ; Hemoglobins - metabolism ; Humans ; Male ; Mass Spectrometry - methods ; Medical sciences ; Middle Aged ; Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis ; Nervous system (semeiology, syndromes) ; Neurology ; Ossification of Posterior Longitudinal Ligament - blood ; Ossification of Posterior Longitudinal Ligament - diagnosis ; Protein Precursors - blood ; Protein Precursors - genetics ; Proteome - metabolism ; Retinol-Binding Proteins - genetics ; Retinol-Binding Proteins - metabolism ; Serum Albumin - genetics ; Serum Albumin - metabolism ; Serum Albumin, Human ; Up-Regulation ; Vascular diseases and vascular malformations of the nervous system</subject><ispartof>Spine (Philadelphia, Pa. 1976), 2007-04, Vol.32 (7), p.728-734</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-aa34993c4649ad1e17dd48aab4746712f25c906e9ee2cdb2f66c9de8b7094d1f3</citedby><cites>FETCH-LOGICAL-c413t-aa34993c4649ad1e17dd48aab4746712f25c906e9ee2cdb2f66c9de8b7094d1f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18654689$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17414905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EUN, Jong-Pil</creatorcontrib><creatorcontrib>MA, Tian-Ze</creatorcontrib><creatorcontrib>LEE, Woo-Jong</creatorcontrib><creatorcontrib>KIM, Min-Gul</creatorcontrib><creatorcontrib>YOO, Min-Jeong</creatorcontrib><creatorcontrib>KOH, Eun-Jung</creatorcontrib><creatorcontrib>CHOI, Ha-Young</creatorcontrib><creatorcontrib>KWAK, Yong-Geun</creatorcontrib><title>Comparative analysis of serum proteomes to discover biomarkers for ossification of the posterior longitudinal ligament</title><title>Spine (Philadelphia, Pa. 1976)</title><addtitle>Spine (Phila Pa 1976)</addtitle><description>Serum proteomes from normal subjects and the patients with ossification of the posterior longitudinal ligament (OPLL) were analyzed by using proteomics.
To identify novel serologic biomarkers for diagnosing OPLL.
OPLL can compress the spinal cord, and special planning is required for surgeries that are done from the front of the cervical spine. However, the definitive serologic biomarkers for OPLL are still unclear.
The 2-dimensional electrophoresis patterns of sera from OPLL patients and normal subjects were compared. The differentially expressed spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization quadruple time-of-flight mass spectrometry.
Nine spots that were differentially expressed in the sera of OPLL patients were found and were identified. PRO2675, human serum albumin in a complex with myristic acid and tri-iodobenzoic acid, an unknown protein, chain B of the crystal structure of deoxy-human hemoglobin beta6, pro-apolipoprotein, ALB protein, retinol binding protein and chain A of human serum albumin mutant R218h complexed with thyroxine (3,3',5,5', tetraiodo-L-thyronine), were up-regulated in the sera of OPLL patients, whereas alpha1-microglobulin/bikunin precursor was down-regulated.
These proteins could be used as diagnostic biomarkers of OPLL.</description><subject>Adult</subject><subject>Apolipoproteins - blood</subject><subject>Apolipoproteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Biomarkers - metabolism</subject><subject>Case-Control Studies</subject><subject>Cerebrospinal fluid. Meninges. Spinal cord</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Female</subject><subject>Glycoproteins - blood</subject><subject>Glycoproteins - genetics</subject><subject>Hemoglobins - genetics</subject><subject>Hemoglobins - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Mass Spectrometry - methods</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis</subject><subject>Nervous system (semeiology, syndromes)</subject><subject>Neurology</subject><subject>Ossification of Posterior Longitudinal Ligament - blood</subject><subject>Ossification of Posterior Longitudinal Ligament - diagnosis</subject><subject>Protein Precursors - blood</subject><subject>Protein Precursors - genetics</subject><subject>Proteome - metabolism</subject><subject>Retinol-Binding Proteins - genetics</subject><subject>Retinol-Binding Proteins - metabolism</subject><subject>Serum Albumin - genetics</subject><subject>Serum Albumin - metabolism</subject><subject>Serum Albumin, Human</subject><subject>Up-Regulation</subject><subject>Vascular diseases and vascular malformations of the nervous system</subject><issn>0362-2436</issn><issn>1528-1159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE9vFCEchomxsdvqVzDERG8zheHf4M1sbGvSxIueCQM_KjozrMBs0m8vtZssFw4870veB6EPlPSUaHVDaD_l0pN2BqGJIr2UIxG9Zq_Qjoph7CgV-jXaESaHbuBMXqKrUn43XjKq36BLqjjlmogdOu7TcrDZ1ngEbFc7P5VYcAq4QN4WfMipQlqg4Jqwj8WlI2Q8xbTY_AdywSFlnEqJIbrWkdbnaP0F-JBKhRzb65zWx1g3H1s5nuOjXWCtb9FFsHOBd6f7Gv28_fpjf989fL_7tv_y0DlOWe2sZVxr5rjk2noKVHnPR2snrrhUdAiDcJpI0ACD89MQpHTawzgpormngV2jTy-9bcjfDUo1SxsB82xXSFsxijAhlFAN_PwCutzmZAjmkGMb-WQoMc_WDaGmWTdn6-a_daNZC78__bJNC_hz9KS5AR9PgC3OziHb1cVy5kYpuBw1-wd9bI93</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>EUN, Jong-Pil</creator><creator>MA, Tian-Ze</creator><creator>LEE, Woo-Jong</creator><creator>KIM, Min-Gul</creator><creator>YOO, Min-Jeong</creator><creator>KOH, Eun-Jung</creator><creator>CHOI, Ha-Young</creator><creator>KWAK, Yong-Geun</creator><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>Comparative analysis of serum proteomes to discover biomarkers for ossification of the posterior longitudinal ligament</title><author>EUN, Jong-Pil ; MA, Tian-Ze ; LEE, Woo-Jong ; KIM, Min-Gul ; YOO, Min-Jeong ; KOH, Eun-Jung ; CHOI, Ha-Young ; KWAK, Yong-Geun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-aa34993c4649ad1e17dd48aab4746712f25c906e9ee2cdb2f66c9de8b7094d1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adult</topic><topic>Apolipoproteins - blood</topic><topic>Apolipoproteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Biomarkers - metabolism</topic><topic>Case-Control Studies</topic><topic>Cerebrospinal fluid. Meninges. Spinal cord</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Female</topic><topic>Glycoproteins - blood</topic><topic>Glycoproteins - genetics</topic><topic>Hemoglobins - genetics</topic><topic>Hemoglobins - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Mass Spectrometry - methods</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis</topic><topic>Nervous system (semeiology, syndromes)</topic><topic>Neurology</topic><topic>Ossification of Posterior Longitudinal Ligament - blood</topic><topic>Ossification of Posterior Longitudinal Ligament - diagnosis</topic><topic>Protein Precursors - blood</topic><topic>Protein Precursors - genetics</topic><topic>Proteome - metabolism</topic><topic>Retinol-Binding Proteins - genetics</topic><topic>Retinol-Binding Proteins - metabolism</topic><topic>Serum Albumin - genetics</topic><topic>Serum Albumin - metabolism</topic><topic>Serum Albumin, Human</topic><topic>Up-Regulation</topic><topic>Vascular diseases and vascular malformations of the nervous system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EUN, Jong-Pil</creatorcontrib><creatorcontrib>MA, Tian-Ze</creatorcontrib><creatorcontrib>LEE, Woo-Jong</creatorcontrib><creatorcontrib>KIM, Min-Gul</creatorcontrib><creatorcontrib>YOO, Min-Jeong</creatorcontrib><creatorcontrib>KOH, Eun-Jung</creatorcontrib><creatorcontrib>CHOI, Ha-Young</creatorcontrib><creatorcontrib>KWAK, Yong-Geun</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Spine (Philadelphia, Pa. 1976)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>EUN, Jong-Pil</au><au>MA, Tian-Ze</au><au>LEE, Woo-Jong</au><au>KIM, Min-Gul</au><au>YOO, Min-Jeong</au><au>KOH, Eun-Jung</au><au>CHOI, Ha-Young</au><au>KWAK, Yong-Geun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative analysis of serum proteomes to discover biomarkers for ossification of the posterior longitudinal ligament</atitle><jtitle>Spine (Philadelphia, Pa. 1976)</jtitle><addtitle>Spine (Phila Pa 1976)</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>32</volume><issue>7</issue><spage>728</spage><epage>734</epage><pages>728-734</pages><issn>0362-2436</issn><eissn>1528-1159</eissn><coden>SPINDD</coden><abstract>Serum proteomes from normal subjects and the patients with ossification of the posterior longitudinal ligament (OPLL) were analyzed by using proteomics.
To identify novel serologic biomarkers for diagnosing OPLL.
OPLL can compress the spinal cord, and special planning is required for surgeries that are done from the front of the cervical spine. However, the definitive serologic biomarkers for OPLL are still unclear.
The 2-dimensional electrophoresis patterns of sera from OPLL patients and normal subjects were compared. The differentially expressed spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and electrospray ionization quadruple time-of-flight mass spectrometry.
Nine spots that were differentially expressed in the sera of OPLL patients were found and were identified. PRO2675, human serum albumin in a complex with myristic acid and tri-iodobenzoic acid, an unknown protein, chain B of the crystal structure of deoxy-human hemoglobin beta6, pro-apolipoprotein, ALB protein, retinol binding protein and chain A of human serum albumin mutant R218h complexed with thyroxine (3,3',5,5', tetraiodo-L-thyronine), were up-regulated in the sera of OPLL patients, whereas alpha1-microglobulin/bikunin precursor was down-regulated.
These proteins could be used as diagnostic biomarkers of OPLL.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>Lippincott</pub><pmid>17414905</pmid><doi>10.1097/01.brs.0000259070.66805.93</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0362-2436 |
| ispartof | Spine (Philadelphia, Pa. 1976), 2007-04, Vol.32 (7), p.728-734 |
| issn | 0362-2436 1528-1159 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70355757 |
| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adult Apolipoproteins - blood Apolipoproteins - genetics Biological and medical sciences Biomarkers - metabolism Case-Control Studies Cerebrospinal fluid. Meninges. Spinal cord Electrophoresis, Gel, Two-Dimensional Female Glycoproteins - blood Glycoproteins - genetics Hemoglobins - genetics Hemoglobins - metabolism Humans Male Mass Spectrometry - methods Medical sciences Middle Aged Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis Nervous system (semeiology, syndromes) Neurology Ossification of Posterior Longitudinal Ligament - blood Ossification of Posterior Longitudinal Ligament - diagnosis Protein Precursors - blood Protein Precursors - genetics Proteome - metabolism Retinol-Binding Proteins - genetics Retinol-Binding Proteins - metabolism Serum Albumin - genetics Serum Albumin - metabolism Serum Albumin, Human Up-Regulation Vascular diseases and vascular malformations of the nervous system |
| title | Comparative analysis of serum proteomes to discover biomarkers for ossification of the posterior longitudinal ligament |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T17%3A44%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20analysis%20of%20serum%20proteomes%20to%20discover%20biomarkers%20for%20ossification%20of%20the%20posterior%20longitudinal%20ligament&rft.jtitle=Spine%20(Philadelphia,%20Pa.%201976)&rft.au=EUN,%20Jong-Pil&rft.date=2007-04-01&rft.volume=32&rft.issue=7&rft.spage=728&rft.epage=734&rft.pages=728-734&rft.issn=0362-2436&rft.eissn=1528-1159&rft.coden=SPINDD&rft_id=info:doi/10.1097/01.brs.0000259070.66805.93&rft_dat=%3Cproquest_cross%3E70355757%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70355757&rft_id=info:pmid/17414905&rfr_iscdi=true |