Initial attachment of osteoblastic cells onto sol-gel derived fluoridated hydroxyapatite coatings

Initial cell attachment and spreading of anchorage‐dependent cells onto the material surface are crucial concerns for the development of more effective implants. In this study, MG63 cells were employed to investigate the initial cell response to sol‐gel derived fluoridated hydroxyapatite (FHA) coatings. Along with that, surface roughness, wettability, and protein adsorption were also characterized for those FHA coatings, respectively. It was observed that both the surface roughness and contact angle have a slight increase in response to the incorporation of more fluorine ions. All FHA coatings showed similar amount of adsorbed proteins (∼1.6 μg/cm2) upon testing in culture medium. Cell counting showed that no significant difference was observed for the amount of initially attached cells between HA and fluoridated HA coatings during the first 4 h culture. On the other hand, the well‐spread cell on all prepared coating surface indicates that the incorporated fluorine ions have no adverse effect on cell spreading process. Therefore, it was suggested from this study that the prepared fluoridated hydroxyapatite coatings have comparable bioactivity to that of pure hydroxyapatite coating, and these results are meaningful for further investigation for application of FHA coatings. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008