Detection of coproantibodies and faecal immune complexes in human trichinellosis
Trichinella spiralis is the nematode causative agent of trichinellosis, an intestinal and tissular parasitosis. Even though an early diagnosis during the intestinal phase is essential to limit the infection in humans, to date, there are no available tests to achieve this goal. Based on the immune re...
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| Veröffentlicht in: | Parasitology 2007-05, Vol.134 (5), p.723-727 |
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| description | Trichinella spiralis is the nematode causative agent of trichinellosis, an intestinal and tissular parasitosis. Even though an early diagnosis during the intestinal phase is essential to limit the infection in humans, to date, there are no available tests to achieve this goal. Based on the immune response generated by the host's intestinal mucosa, the aim of this work was to develop ELISAs to assess the presence of coproantigens (CAgs), coproantibodies (CAbs) and faecal immune complexes in stool samples of 18 individuals belonging to different outbreaks that have arisen in Argentina. By the methodologies developed in this work it was found that anti-muscle larva excretory-secretory products (ML-ESP) CAbs were detected in 89% of the samples analysed regardless of the time p.i. Anti-ML-ESP IgA, IgG, IgE and IgM were detected in 56%, 56%, 28% and 22% of the individuals respectively. Those samples negative for anti-ML-ESP total immunoglobulins proved positive for anti-adult worm-ESP CAbs. No CAgs were detected in any of the samples. The results obtained in this work indicate that the intestinal immune response in human trichinellosis is featured by all the isotypes of specific immunoglobulins. Furthermore, the detection of antibodies in stool samples, in either the free or complexed form, could be applied to confirm early human trichinellosis. |
| doi_str_mv | 10.1017/S0031182006001983 |
| format | Article |
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G. ; COSTANTINO, S. N. ; VENTURIELLO, S. M.</creator><creatorcontrib>NUÑEZ, G. G. ; COSTANTINO, S. N. ; VENTURIELLO, S. M.</creatorcontrib><description>Trichinella spiralis is the nematode causative agent of trichinellosis, an intestinal and tissular parasitosis. Even though an early diagnosis during the intestinal phase is essential to limit the infection in humans, to date, there are no available tests to achieve this goal. Based on the immune response generated by the host's intestinal mucosa, the aim of this work was to develop ELISAs to assess the presence of coproantigens (CAgs), coproantibodies (CAbs) and faecal immune complexes in stool samples of 18 individuals belonging to different outbreaks that have arisen in Argentina. By the methodologies developed in this work it was found that anti-muscle larva excretory-secretory products (ML-ESP) CAbs were detected in 89% of the samples analysed regardless of the time p.i. Anti-ML-ESP IgA, IgG, IgE and IgM were detected in 56%, 56%, 28% and 22% of the individuals respectively. Those samples negative for anti-ML-ESP total immunoglobulins proved positive for anti-adult worm-ESP CAbs. No CAgs were detected in any of the samples. The results obtained in this work indicate that the intestinal immune response in human trichinellosis is featured by all the isotypes of specific immunoglobulins. Furthermore, the detection of antibodies in stool samples, in either the free or complexed form, could be applied to confirm early human trichinellosis.</description><identifier>ISSN: 0031-1820</identifier><identifier>EISSN: 1469-8161</identifier><identifier>DOI: 10.1017/S0031182006001983</identifier><identifier>PMID: 17156581</identifier><identifier>CODEN: PARAAE</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Antibodies, Helminth - analysis ; Biological and medical sciences ; coproantibodies ; coproantigens ; Enzyme-Linked Immunosorbent Assay ; faecal immune complexes ; Feces - chemistry ; Feces - parasitology ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; human trichinellosis ; Humans ; Immune response ; immunoglobulin isotypes ; Immunoglobulins - analysis ; Invertebrates ; Nematoda ; Trichinella spiralis ; Trichinellosis - diagnosis ; Trichinellosis - immunology</subject><ispartof>Parasitology, 2007-05, Vol.134 (5), p.723-727</ispartof><rights>Copyright © Cambridge University Press 2006</rights><rights>2007 INIST-CNRS</rights><rights>Cambridge University Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c469t-289654efe4253958b64bd39cecfc2273fa2e343e3450d436fe14cc3938d5aab73</citedby><cites>FETCH-LOGICAL-c469t-289654efe4253958b64bd39cecfc2273fa2e343e3450d436fe14cc3938d5aab73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0031182006001983/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18753053$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17156581$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NUÑEZ, G. G.</creatorcontrib><creatorcontrib>COSTANTINO, S. N.</creatorcontrib><creatorcontrib>VENTURIELLO, S. M.</creatorcontrib><title>Detection of coproantibodies and faecal immune complexes in human trichinellosis</title><title>Parasitology</title><addtitle>Parasitology</addtitle><description>Trichinella spiralis is the nematode causative agent of trichinellosis, an intestinal and tissular parasitosis. Even though an early diagnosis during the intestinal phase is essential to limit the infection in humans, to date, there are no available tests to achieve this goal. Based on the immune response generated by the host's intestinal mucosa, the aim of this work was to develop ELISAs to assess the presence of coproantigens (CAgs), coproantibodies (CAbs) and faecal immune complexes in stool samples of 18 individuals belonging to different outbreaks that have arisen in Argentina. By the methodologies developed in this work it was found that anti-muscle larva excretory-secretory products (ML-ESP) CAbs were detected in 89% of the samples analysed regardless of the time p.i. Anti-ML-ESP IgA, IgG, IgE and IgM were detected in 56%, 56%, 28% and 22% of the individuals respectively. Those samples negative for anti-ML-ESP total immunoglobulins proved positive for anti-adult worm-ESP CAbs. No CAgs were detected in any of the samples. The results obtained in this work indicate that the intestinal immune response in human trichinellosis is featured by all the isotypes of specific immunoglobulins. Furthermore, the detection of antibodies in stool samples, in either the free or complexed form, could be applied to confirm early human trichinellosis.</description><subject>Animals</subject><subject>Antibodies, Helminth - analysis</subject><subject>Biological and medical sciences</subject><subject>coproantibodies</subject><subject>coproantigens</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>faecal immune complexes</subject><subject>Feces - chemistry</subject><subject>Feces - parasitology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>human trichinellosis</subject><subject>Humans</subject><subject>Immune response</subject><subject>immunoglobulin isotypes</subject><subject>Immunoglobulins - analysis</subject><subject>Invertebrates</subject><subject>Nematoda</subject><subject>Trichinella spiralis</subject><subject>Trichinellosis - diagnosis</subject><subject>Trichinellosis - immunology</subject><issn>0031-1820</issn><issn>1469-8161</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNqFkUtv1TAQhS0EopfCD2CDIqSyC9ie-JElurQF0YoiHlvLcSbUJXEudiKVf4-jG3ElKtSF5cX55ujMHEKeM_qaUabefKEUGNOcUkkpqzU8IBtWybrUTLKHZLPI5aIfkScp3dDMgeSPyRFTTEih2YZcvcMJ3eTHUIxd4cZdHG2YfDO2HlNhQ1t0Fp3tCz8Mc8BMDLseb7PmQ3E9DzYUU_Tu2gfs-zH59JQ86myf8Nn6H5NvZ6dft-_Li0_nH7ZvL0qXA04l17UUFXZYcQG10I2smhZqh65znCvoLEeoID9B2wpkh6xyDmrQrbC2UXBMXu19c-JfM6bJDD65HMIGHOdkFAUBrOb3gpwqVSu2gC__AW_GOYa8hOH5qrzSHDLE9pCLY0oRO7OLfrDxt2HULKWYO6XkmRer8dwM2B4m1hYycLICNuVbd9EG59OB00oAFYtRued8mvD2r27jTyMVKGHk-WdzuaVXHy_huznLPKxh7dBE3_7Aw0r_j_sHzCOxWg</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>NUÑEZ, G. G.</creator><creator>COSTANTINO, S. N.</creator><creator>VENTURIELLO, S. 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G. ; COSTANTINO, S. N. ; VENTURIELLO, S. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c469t-289654efe4253958b64bd39cecfc2273fa2e343e3450d436fe14cc3938d5aab73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antibodies, Helminth - analysis</topic><topic>Biological and medical sciences</topic><topic>coproantibodies</topic><topic>coproantigens</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>faecal immune complexes</topic><topic>Feces - chemistry</topic><topic>Feces - parasitology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>General aspects and techniques. Study of several systematic groups. Models</topic><topic>human trichinellosis</topic><topic>Humans</topic><topic>Immune response</topic><topic>immunoglobulin isotypes</topic><topic>Immunoglobulins - analysis</topic><topic>Invertebrates</topic><topic>Nematoda</topic><topic>Trichinella spiralis</topic><topic>Trichinellosis - diagnosis</topic><topic>Trichinellosis - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NUÑEZ, G. G.</creatorcontrib><creatorcontrib>COSTANTINO, S. N.</creatorcontrib><creatorcontrib>VENTURIELLO, S. 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G.</au><au>COSTANTINO, S. N.</au><au>VENTURIELLO, S. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of coproantibodies and faecal immune complexes in human trichinellosis</atitle><jtitle>Parasitology</jtitle><addtitle>Parasitology</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>134</volume><issue>5</issue><spage>723</spage><epage>727</epage><pages>723-727</pages><issn>0031-1820</issn><eissn>1469-8161</eissn><coden>PARAAE</coden><abstract>Trichinella spiralis is the nematode causative agent of trichinellosis, an intestinal and tissular parasitosis. Even though an early diagnosis during the intestinal phase is essential to limit the infection in humans, to date, there are no available tests to achieve this goal. Based on the immune response generated by the host's intestinal mucosa, the aim of this work was to develop ELISAs to assess the presence of coproantigens (CAgs), coproantibodies (CAbs) and faecal immune complexes in stool samples of 18 individuals belonging to different outbreaks that have arisen in Argentina. By the methodologies developed in this work it was found that anti-muscle larva excretory-secretory products (ML-ESP) CAbs were detected in 89% of the samples analysed regardless of the time p.i. Anti-ML-ESP IgA, IgG, IgE and IgM were detected in 56%, 56%, 28% and 22% of the individuals respectively. Those samples negative for anti-ML-ESP total immunoglobulins proved positive for anti-adult worm-ESP CAbs. No CAgs were detected in any of the samples. The results obtained in this work indicate that the intestinal immune response in human trichinellosis is featured by all the isotypes of specific immunoglobulins. Furthermore, the detection of antibodies in stool samples, in either the free or complexed form, could be applied to confirm early human trichinellosis.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>17156581</pmid><doi>10.1017/S0031182006001983</doi><tpages>5</tpages></addata></record> |
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| subjects | Animals Antibodies, Helminth - analysis Biological and medical sciences coproantibodies coproantigens Enzyme-Linked Immunosorbent Assay faecal immune complexes Feces - chemistry Feces - parasitology Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models human trichinellosis Humans Immune response immunoglobulin isotypes Immunoglobulins - analysis Invertebrates Nematoda Trichinella spiralis Trichinellosis - diagnosis Trichinellosis - immunology |
| title | Detection of coproantibodies and faecal immune complexes in human trichinellosis |
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