Efficient expansion of clinical-grade human fibroblasts on microcarriers: Cells suitable for ex vivo expansion of clinical-grade hESCs

Human embryonic stem cells hold considerable potential for cell-based treatments of a variety of degenerative diseases, including diabetes, ischemic heart failure, and Parkinson's disease. However, advancing research to provide clinical-grade product requires scale-up to therapeutic quantities...

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Veröffentlicht in:	Journal of biotechnology 2008-03, Vol.134 (1), p.79-87
Hauptverfasser:	Phillips, Blaine Wesley, Lim, Ricky Yee Meng, Tan, Thong Teck, Rust, William Lathrop, Crook, Jeremy Micah
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cell Culture Techniques - methods Cells, Cultured Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism ES cells Fibroblasts - cytology Fibroblasts - metabolism Flow Cytometry Fundamental and applied biological sciences. Psychology Human embryonic stem cells Humans Immunohistochemistry Microcarriers Reverse Transcriptase Polymerase Chain Reaction Scale-up
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