Isolation of Primary Human Hepatocytes After Partial Hepatectomy: Criteria for Identification of the Most Promising Liver Specimen
: Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve cell availability, the present study investigates the influence of donor liver characteristics on the outcome of hepatocyte isolation from surgically removed liv...
Gespeichert in:
| Veröffentlicht in: | Artificial organs 2008-03, Vol.32 (3), p.205-213 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 213 |
|---|---|
| container_issue | 3 |
| container_start_page | 205 |
| container_title | Artificial organs |
| container_volume | 32 |
| creator | Vondran, Florian Wolfgang Rudolf Katenz, Ekaterina Schwartlander, Ruth Morgul, Mehmet Haluk Raschzok, Nathanael Gong, Xiaobing Cheng, Xiangdong Kehr, Daniel Sauer, Igor Maximilian |
| description | : Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve cell availability, the present study investigates the influence of donor liver characteristics on the outcome of hepatocyte isolation from surgically removed liver tissue (n = 50). Hepatocytes were isolated from liver specimens using a standardized two‐step collagenase perfusion technique. The patient's sex, previous chemotherapy, or histopathology have shown no influence. Donor age significantly affected the isolation outcome, but was not found suitable for predicting cell yields. Preoperative blood parameters did not correlate with cell yield, although cell function was affected: total protein, albumin synthesis, and cell viability were significantly decreased for serum gamma‐glutamyl‐transferase (GGT) levels >60 U/L. Specimens from patients with benign diseases gave significantly higher cell yields than tissue removed due to secondary and primary tumors, respectively. The indication for surgery is a valuable basis for identifying the most yielding specimens. Hepatocytes from donors with high GGT levels appear to show reduced functional properties. |
| doi_str_mv | 10.1111/j.1525-1594.2007.00524.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70345811</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70345811</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4544-a9a08eefadcf04dc2b863ba066408b0677c3fbf5ce0875bd39041717a6a5b8ee3</originalsourceid><addsrcrecordid>eNqNkMGO0zAURS0EYsrALyCv2CU8J3aSIjalYqaVCjMDg2BnOe4zuCRxsV1ot3w5LqnKFm9s6d1zn3UIoQxyls7LTc5EITImpjwvAOocQBQ83z8gk_PgIZkAqyATFf9yQZ6EsIGU5FA9JhesKYAVTTMhv5fBdSpaN1Bn6K23vfIHutj1aqAL3Kro9CFioDMT0dNb5aNV3ThBHV1_eEXn3qaZVdQ4T5drHKI1Vp874zek71yIqdz1NtjhK13Zn6ns4xa17XF4Sh4Z1QV8drovyaert_fzRba6uV7OZ6tMc8F5pqYKGkSj1toAX-uibaqyVVBVHJoWqrrWpWmN0AhNLdp1OQXOalarSok2geUleTH2br37scMQZfqOxq5TA7pdkDWUXDSMpWAzBrV3IXg0cjt6kQzk0b_cyKNmedQsj_7lX_9yn9Dnpx27tsf1P_AkPAVej4FftsPDfxfL2c2H9Eh4NuI2RNyfceW_y6ouayE_v7-WV2_Y_O5uei9F-QeahqUu</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70345811</pqid></control><display><type>article</type><title>Isolation of Primary Human Hepatocytes After Partial Hepatectomy: Criteria for Identification of the Most Promising Liver Specimen</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Vondran, Florian Wolfgang Rudolf ; Katenz, Ekaterina ; Schwartlander, Ruth ; Morgul, Mehmet Haluk ; Raschzok, Nathanael ; Gong, Xiaobing ; Cheng, Xiangdong ; Kehr, Daniel ; Sauer, Igor Maximilian</creator><creatorcontrib>Vondran, Florian Wolfgang Rudolf ; Katenz, Ekaterina ; Schwartlander, Ruth ; Morgul, Mehmet Haluk ; Raschzok, Nathanael ; Gong, Xiaobing ; Cheng, Xiangdong ; Kehr, Daniel ; Sauer, Igor Maximilian</creatorcontrib><description>: Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve cell availability, the present study investigates the influence of donor liver characteristics on the outcome of hepatocyte isolation from surgically removed liver tissue (n = 50). Hepatocytes were isolated from liver specimens using a standardized two‐step collagenase perfusion technique. The patient's sex, previous chemotherapy, or histopathology have shown no influence. Donor age significantly affected the isolation outcome, but was not found suitable for predicting cell yields. Preoperative blood parameters did not correlate with cell yield, although cell function was affected: total protein, albumin synthesis, and cell viability were significantly decreased for serum gamma‐glutamyl‐transferase (GGT) levels >60 U/L. Specimens from patients with benign diseases gave significantly higher cell yields than tissue removed due to secondary and primary tumors, respectively. The indication for surgery is a valuable basis for identifying the most yielding specimens. Hepatocytes from donors with high GGT levels appear to show reduced functional properties.</description><identifier>ISSN: 0160-564X</identifier><identifier>EISSN: 1525-1594</identifier><identifier>DOI: 10.1111/j.1525-1594.2007.00524.x</identifier><identifier>PMID: 18201288</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>Adult ; Aged ; Albumins - biosynthesis ; Aspartate Aminotransferases - metabolism ; Cell Culture Techniques ; Cell Separation ; Cell Shape ; Cell Survival ; Cells, Cultured ; Characteristics ; Donor ; Female ; Function ; gamma-Glutamyltransferase - blood ; Hepatectomy ; Hepatocyte ; Hepatocytes - enzymology ; Hepatocytes - metabolism ; Hepatocytes - pathology ; Human ; Humans ; Isolation ; L-Lactate Dehydrogenase - metabolism ; Liver ; Liver - enzymology ; Liver - metabolism ; Liver - pathology ; Liver - surgery ; Male ; Middle Aged ; Outcome ; Protein Biosynthesis ; Time Factors ; Urea - metabolism</subject><ispartof>Artificial organs, 2008-03, Vol.32 (3), p.205-213</ispartof><rights>2007, Copyright the Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4544-a9a08eefadcf04dc2b863ba066408b0677c3fbf5ce0875bd39041717a6a5b8ee3</citedby><cites>FETCH-LOGICAL-c4544-a9a08eefadcf04dc2b863ba066408b0677c3fbf5ce0875bd39041717a6a5b8ee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1525-1594.2007.00524.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1525-1594.2007.00524.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18201288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vondran, Florian Wolfgang Rudolf</creatorcontrib><creatorcontrib>Katenz, Ekaterina</creatorcontrib><creatorcontrib>Schwartlander, Ruth</creatorcontrib><creatorcontrib>Morgul, Mehmet Haluk</creatorcontrib><creatorcontrib>Raschzok, Nathanael</creatorcontrib><creatorcontrib>Gong, Xiaobing</creatorcontrib><creatorcontrib>Cheng, Xiangdong</creatorcontrib><creatorcontrib>Kehr, Daniel</creatorcontrib><creatorcontrib>Sauer, Igor Maximilian</creatorcontrib><title>Isolation of Primary Human Hepatocytes After Partial Hepatectomy: Criteria for Identification of the Most Promising Liver Specimen</title><title>Artificial organs</title><addtitle>Artif Organs</addtitle><description>: Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve cell availability, the present study investigates the influence of donor liver characteristics on the outcome of hepatocyte isolation from surgically removed liver tissue (n = 50). Hepatocytes were isolated from liver specimens using a standardized two‐step collagenase perfusion technique. The patient's sex, previous chemotherapy, or histopathology have shown no influence. Donor age significantly affected the isolation outcome, but was not found suitable for predicting cell yields. Preoperative blood parameters did not correlate with cell yield, although cell function was affected: total protein, albumin synthesis, and cell viability were significantly decreased for serum gamma‐glutamyl‐transferase (GGT) levels >60 U/L. Specimens from patients with benign diseases gave significantly higher cell yields than tissue removed due to secondary and primary tumors, respectively. The indication for surgery is a valuable basis for identifying the most yielding specimens. Hepatocytes from donors with high GGT levels appear to show reduced functional properties.</description><subject>Adult</subject><subject>Aged</subject><subject>Albumins - biosynthesis</subject><subject>Aspartate Aminotransferases - metabolism</subject><subject>Cell Culture Techniques</subject><subject>Cell Separation</subject><subject>Cell Shape</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Characteristics</subject><subject>Donor</subject><subject>Female</subject><subject>Function</subject><subject>gamma-Glutamyltransferase - blood</subject><subject>Hepatectomy</subject><subject>Hepatocyte</subject><subject>Hepatocytes - enzymology</subject><subject>Hepatocytes - metabolism</subject><subject>Hepatocytes - pathology</subject><subject>Human</subject><subject>Humans</subject><subject>Isolation</subject><subject>L-Lactate Dehydrogenase - metabolism</subject><subject>Liver</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Liver - pathology</subject><subject>Liver - surgery</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Outcome</subject><subject>Protein Biosynthesis</subject><subject>Time Factors</subject><subject>Urea - metabolism</subject><issn>0160-564X</issn><issn>1525-1594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMGO0zAURS0EYsrALyCv2CU8J3aSIjalYqaVCjMDg2BnOe4zuCRxsV1ot3w5LqnKFm9s6d1zn3UIoQxyls7LTc5EITImpjwvAOocQBQ83z8gk_PgIZkAqyATFf9yQZ6EsIGU5FA9JhesKYAVTTMhv5fBdSpaN1Bn6K23vfIHutj1aqAL3Kro9CFioDMT0dNb5aNV3ThBHV1_eEXn3qaZVdQ4T5drHKI1Vp874zek71yIqdz1NtjhK13Zn6ns4xa17XF4Sh4Z1QV8drovyaert_fzRba6uV7OZ6tMc8F5pqYKGkSj1toAX-uibaqyVVBVHJoWqrrWpWmN0AhNLdp1OQXOalarSok2geUleTH2br37scMQZfqOxq5TA7pdkDWUXDSMpWAzBrV3IXg0cjt6kQzk0b_cyKNmedQsj_7lX_9yn9Dnpx27tsf1P_AkPAVej4FftsPDfxfL2c2H9Eh4NuI2RNyfceW_y6ouayE_v7-WV2_Y_O5uei9F-QeahqUu</recordid><startdate>200803</startdate><enddate>200803</enddate><creator>Vondran, Florian Wolfgang Rudolf</creator><creator>Katenz, Ekaterina</creator><creator>Schwartlander, Ruth</creator><creator>Morgul, Mehmet Haluk</creator><creator>Raschzok, Nathanael</creator><creator>Gong, Xiaobing</creator><creator>Cheng, Xiangdong</creator><creator>Kehr, Daniel</creator><creator>Sauer, Igor Maximilian</creator><general>Blackwell Publishing Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200803</creationdate><title>Isolation of Primary Human Hepatocytes After Partial Hepatectomy: Criteria for Identification of the Most Promising Liver Specimen</title><author>Vondran, Florian Wolfgang Rudolf ; Katenz, Ekaterina ; Schwartlander, Ruth ; Morgul, Mehmet Haluk ; Raschzok, Nathanael ; Gong, Xiaobing ; Cheng, Xiangdong ; Kehr, Daniel ; Sauer, Igor Maximilian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4544-a9a08eefadcf04dc2b863ba066408b0677c3fbf5ce0875bd39041717a6a5b8ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Albumins - biosynthesis</topic><topic>Aspartate Aminotransferases - metabolism</topic><topic>Cell Culture Techniques</topic><topic>Cell Separation</topic><topic>Cell Shape</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Characteristics</topic><topic>Donor</topic><topic>Female</topic><topic>Function</topic><topic>gamma-Glutamyltransferase - blood</topic><topic>Hepatectomy</topic><topic>Hepatocyte</topic><topic>Hepatocytes - enzymology</topic><topic>Hepatocytes - metabolism</topic><topic>Hepatocytes - pathology</topic><topic>Human</topic><topic>Humans</topic><topic>Isolation</topic><topic>L-Lactate Dehydrogenase - metabolism</topic><topic>Liver</topic><topic>Liver - enzymology</topic><topic>Liver - metabolism</topic><topic>Liver - pathology</topic><topic>Liver - surgery</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Outcome</topic><topic>Protein Biosynthesis</topic><topic>Time Factors</topic><topic>Urea - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vondran, Florian Wolfgang Rudolf</creatorcontrib><creatorcontrib>Katenz, Ekaterina</creatorcontrib><creatorcontrib>Schwartlander, Ruth</creatorcontrib><creatorcontrib>Morgul, Mehmet Haluk</creatorcontrib><creatorcontrib>Raschzok, Nathanael</creatorcontrib><creatorcontrib>Gong, Xiaobing</creatorcontrib><creatorcontrib>Cheng, Xiangdong</creatorcontrib><creatorcontrib>Kehr, Daniel</creatorcontrib><creatorcontrib>Sauer, Igor Maximilian</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Artificial organs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vondran, Florian Wolfgang Rudolf</au><au>Katenz, Ekaterina</au><au>Schwartlander, Ruth</au><au>Morgul, Mehmet Haluk</au><au>Raschzok, Nathanael</au><au>Gong, Xiaobing</au><au>Cheng, Xiangdong</au><au>Kehr, Daniel</au><au>Sauer, Igor Maximilian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of Primary Human Hepatocytes After Partial Hepatectomy: Criteria for Identification of the Most Promising Liver Specimen</atitle><jtitle>Artificial organs</jtitle><addtitle>Artif Organs</addtitle><date>2008-03</date><risdate>2008</risdate><volume>32</volume><issue>3</issue><spage>205</spage><epage>213</epage><pages>205-213</pages><issn>0160-564X</issn><eissn>1525-1594</eissn><abstract>: Demands for primary human hepatocytes are continuously increasing, while supply is insufficient due to limited cell sources. To improve cell availability, the present study investigates the influence of donor liver characteristics on the outcome of hepatocyte isolation from surgically removed liver tissue (n = 50). Hepatocytes were isolated from liver specimens using a standardized two‐step collagenase perfusion technique. The patient's sex, previous chemotherapy, or histopathology have shown no influence. Donor age significantly affected the isolation outcome, but was not found suitable for predicting cell yields. Preoperative blood parameters did not correlate with cell yield, although cell function was affected: total protein, albumin synthesis, and cell viability were significantly decreased for serum gamma‐glutamyl‐transferase (GGT) levels >60 U/L. Specimens from patients with benign diseases gave significantly higher cell yields than tissue removed due to secondary and primary tumors, respectively. The indication for surgery is a valuable basis for identifying the most yielding specimens. Hepatocytes from donors with high GGT levels appear to show reduced functional properties.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>18201288</pmid><doi>10.1111/j.1525-1594.2007.00524.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0160-564X |
| ispartof | Artificial organs, 2008-03, Vol.32 (3), p.205-213 |
| issn | 0160-564X 1525-1594 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70345811 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Adult Aged Albumins - biosynthesis Aspartate Aminotransferases - metabolism Cell Culture Techniques Cell Separation Cell Shape Cell Survival Cells, Cultured Characteristics Donor Female Function gamma-Glutamyltransferase - blood Hepatectomy Hepatocyte Hepatocytes - enzymology Hepatocytes - metabolism Hepatocytes - pathology Human Humans Isolation L-Lactate Dehydrogenase - metabolism Liver Liver - enzymology Liver - metabolism Liver - pathology Liver - surgery Male Middle Aged Outcome Protein Biosynthesis Time Factors Urea - metabolism |
| title | Isolation of Primary Human Hepatocytes After Partial Hepatectomy: Criteria for Identification of the Most Promising Liver Specimen |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T00%3A14%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Isolation%20of%20Primary%20Human%20Hepatocytes%20After%20Partial%20Hepatectomy:%20Criteria%20for%20Identification%20of%20the%20Most%20Promising%20Liver%20Specimen&rft.jtitle=Artificial%20organs&rft.au=Vondran,%20Florian%20Wolfgang%20Rudolf&rft.date=2008-03&rft.volume=32&rft.issue=3&rft.spage=205&rft.epage=213&rft.pages=205-213&rft.issn=0160-564X&rft.eissn=1525-1594&rft_id=info:doi/10.1111/j.1525-1594.2007.00524.x&rft_dat=%3Cproquest_cross%3E70345811%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70345811&rft_id=info:pmid/18201288&rfr_iscdi=true |