Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish
The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei. Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to...
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| Veröffentlicht in: | Genetica 2007-05, Vol.130 (1), p.9-17 |
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| creator | Pisano, Eva Coscia, Maria Rosaria Mazzei, Federico Ghigliotti, Laura Coutanceau, Jean-Pierre Ozouf-Costaz, Catherine Oreste, Umberto |
| description | The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei. Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci. RT-PCR experiments revealed the presence of three allelic sequences in T. newnesi, a species in which the IgH genes were organized in two chromosomal loci. Possible pathways leading to IgH genes duplication during the diversification of trematomine fishes were inferred from the analysis of the FISH patterns in a phylogenetic context. The present work provides the first comprehensive picture of IgH genes organization at chromosomal level in a bony fish group. |
| doi_str_mv | 10.1007/s10709-006-0015-4 |
| format | Article |
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Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci. RT-PCR experiments revealed the presence of three allelic sequences in T. newnesi, a species in which the IgH genes were organized in two chromosomal loci. Possible pathways leading to IgH genes duplication during the diversification of trematomine fishes were inferred from the analysis of the FISH patterns in a phylogenetic context. The present work provides the first comprehensive picture of IgH genes organization at chromosomal level in a bony fish group.</description><identifier>ISSN: 0016-6707</identifier><identifier>EISSN: 1573-6857</identifier><identifier>DOI: 10.1007/s10709-006-0015-4</identifier><identifier>PMID: 16909334</identifier><language>eng</language><publisher>Netherlands: Dordrecht : Kluwer Academic Publishers</publisher><subject>Animals ; Antarctic fish ; Antarctic Regions ; Chromosome Mapping ; Chromosomes ; Cytogenetic mapping ; DNA, Complementary - analysis ; Fish ; Genes, Immunoglobulin Heavy Chain ; Genomics ; IgH locus ; In Situ Hybridization, Fluorescence ; Nototheniidae ; Notothenioidei ; Perciformes - genetics ; Perciformes - immunology ; Phylogeny ; Trematominae ; Trematomus ; Trematomus bernacchii</subject><ispartof>Genetica, 2007-05, Vol.130 (1), p.9-17</ispartof><rights>Springer Science+Business Media, Inc. 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-bd8a6dadd859f16decd8889ff95f9f20e05297eaffa6c227e9e2821480582d4c3</citedby><cites>FETCH-LOGICAL-c381t-bd8a6dadd859f16decd8889ff95f9f20e05297eaffa6c227e9e2821480582d4c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16909334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pisano, Eva</creatorcontrib><creatorcontrib>Coscia, Maria Rosaria</creatorcontrib><creatorcontrib>Mazzei, Federico</creatorcontrib><creatorcontrib>Ghigliotti, Laura</creatorcontrib><creatorcontrib>Coutanceau, Jean-Pierre</creatorcontrib><creatorcontrib>Ozouf-Costaz, Catherine</creatorcontrib><creatorcontrib>Oreste, Umberto</creatorcontrib><title>Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish</title><title>Genetica</title><addtitle>Genetica</addtitle><description>The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei. Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci. RT-PCR experiments revealed the presence of three allelic sequences in T. newnesi, a species in which the IgH genes were organized in two chromosomal loci. Possible pathways leading to IgH genes duplication during the diversification of trematomine fishes were inferred from the analysis of the FISH patterns in a phylogenetic context. The present work provides the first comprehensive picture of IgH genes organization at chromosomal level in a bony fish group.</description><subject>Animals</subject><subject>Antarctic fish</subject><subject>Antarctic Regions</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Cytogenetic mapping</subject><subject>DNA, Complementary - analysis</subject><subject>Fish</subject><subject>Genes, Immunoglobulin Heavy Chain</subject><subject>Genomics</subject><subject>IgH locus</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Nototheniidae</subject><subject>Notothenioidei</subject><subject>Perciformes - genetics</subject><subject>Perciformes - immunology</subject><subject>Phylogeny</subject><subject>Trematominae</subject><subject>Trematomus</subject><subject>Trematomus bernacchii</subject><issn>0016-6707</issn><issn>1573-6857</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkV1LwzAUhoMobk5_gDdavPCuepK0-biU4cdg4IXuOmRp0nW0TW1aYf_ejA0Eb7wIORye903OeRG6xvCAAfhjwMBBpgAsHpyn2Qma4pzTlImcn6JpbLKUceATdBHCFgAkZ_IcTTCTICnNpmgx3w2-tK0dKpM0uuuqtky8S6qmGVtf1n491lWbbKz-3iVmo2O9p0MSi6d20L3ZC10VNpfozOk62KvjPUOrl-fP-Vu6fH9dzJ-WqaECD-m6EJoVuihELh1mhTWFEEI6J3MnHQELOZHcauc0M4RwKy0RBGcCckGKzNAZuj_4dr3_Gm0YVFMFY-tat9aPQXGgWRxN_AtiyaigIo_g3R9w68e-jUMoziinMr4cIXyATO9D6K1TXV81ut8pDGqfhjqkoWIaap-GyqLm5mg8rhtb_CqO64_A7QFw2itd9lVQqw8CmEa_-DlC6Q9_po4G</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Pisano, Eva</creator><creator>Coscia, Maria Rosaria</creator><creator>Mazzei, Federico</creator><creator>Ghigliotti, Laura</creator><creator>Coutanceau, Jean-Pierre</creator><creator>Ozouf-Costaz, Catherine</creator><creator>Oreste, Umberto</creator><general>Dordrecht : Kluwer Academic Publishers</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SN</scope><scope>7SS</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20070501</creationdate><title>Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish</title><author>Pisano, Eva ; Coscia, Maria Rosaria ; Mazzei, Federico ; Ghigliotti, Laura ; Coutanceau, Jean-Pierre ; Ozouf-Costaz, Catherine ; Oreste, Umberto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-bd8a6dadd859f16decd8889ff95f9f20e05297eaffa6c227e9e2821480582d4c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Antarctic fish</topic><topic>Antarctic Regions</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Cytogenetic mapping</topic><topic>DNA, Complementary - analysis</topic><topic>Fish</topic><topic>Genes, Immunoglobulin Heavy Chain</topic><topic>Genomics</topic><topic>IgH locus</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Nototheniidae</topic><topic>Notothenioidei</topic><topic>Perciformes - genetics</topic><topic>Perciformes - immunology</topic><topic>Phylogeny</topic><topic>Trematominae</topic><topic>Trematomus</topic><topic>Trematomus bernacchii</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pisano, Eva</creatorcontrib><creatorcontrib>Coscia, Maria Rosaria</creatorcontrib><creatorcontrib>Mazzei, Federico</creatorcontrib><creatorcontrib>Ghigliotti, Laura</creatorcontrib><creatorcontrib>Coutanceau, Jean-Pierre</creatorcontrib><creatorcontrib>Ozouf-Costaz, Catherine</creatorcontrib><creatorcontrib>Oreste, Umberto</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genetica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pisano, Eva</au><au>Coscia, Maria Rosaria</au><au>Mazzei, Federico</au><au>Ghigliotti, Laura</au><au>Coutanceau, Jean-Pierre</au><au>Ozouf-Costaz, Catherine</au><au>Oreste, Umberto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish</atitle><jtitle>Genetica</jtitle><addtitle>Genetica</addtitle><date>2007-05-01</date><risdate>2007</risdate><volume>130</volume><issue>1</issue><spage>9</spage><epage>17</epage><pages>9-17</pages><issn>0016-6707</issn><eissn>1573-6857</eissn><abstract>The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei. Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci. RT-PCR experiments revealed the presence of three allelic sequences in T. newnesi, a species in which the IgH genes were organized in two chromosomal loci. Possible pathways leading to IgH genes duplication during the diversification of trematomine fishes were inferred from the analysis of the FISH patterns in a phylogenetic context. The present work provides the first comprehensive picture of IgH genes organization at chromosomal level in a bony fish group.</abstract><cop>Netherlands</cop><pub>Dordrecht : Kluwer Academic Publishers</pub><pmid>16909334</pmid><doi>10.1007/s10709-006-0015-4</doi><tpages>9</tpages></addata></record> |
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| ispartof | Genetica, 2007-05, Vol.130 (1), p.9-17 |
| issn | 0016-6707 1573-6857 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Antarctic fish Antarctic Regions Chromosome Mapping Chromosomes Cytogenetic mapping DNA, Complementary - analysis Fish Genes, Immunoglobulin Heavy Chain Genomics IgH locus In Situ Hybridization, Fluorescence Nototheniidae Notothenioidei Perciformes - genetics Perciformes - immunology Phylogeny Trematominae Trematomus Trematomus bernacchii |
| title | Cytogenetic mapping of immunoglobulin heavy chain genes in Antarctic fish |
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