Meta and Para Effects in the Ultrafast Excited-State Dynamics of the Green Fluorescent Protein Chromophores
Femtosecond transient absorption and fluorescence upconversion experiments have been performed to investigate the photoinduced dynamics of the meta isomer of the green fluorescent protein chromophore, m-HBDI, and its O-methylated derivative, m-MeOBDI, in various solvent mixtures at neutral, acidic,...
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| Veröffentlicht in: | The journal of physical chemistry. B 2008-03, Vol.112 (9), p.2700-2711 |
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| container_end_page | 2711 |
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| container_title | The journal of physical chemistry. B |
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| creator | Solntsev, Kyril M Poizat, Olivier Dong, Jian Rehault, Julien Lou, Yongbing Burda, Clemens Tolbert, Laren M |
| description | Femtosecond transient absorption and fluorescence upconversion experiments have been performed to investigate the photoinduced dynamics of the meta isomer of the green fluorescent protein chromophore, m-HBDI, and its O-methylated derivative, m-MeOBDI, in various solvent mixtures at neutral, acidic, and basic pH. The para isomer, p-HBDI, and its O- and N-methylated derivatives, p-MeOBDI and p-HBDIMe+, were also studied for comparison. In all cases, fast quenching of the excited S1 state by internal conversion (IC) to the ground state was observed. In the para compounds, IC, presumably promoted by the internal twisting, arises in |
| doi_str_mv | 10.1021/jp077707t |
| format | Article |
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The para isomer, p-HBDI, and its O- and N-methylated derivatives, p-MeOBDI and p-HBDIMe+, were also studied for comparison. In all cases, fast quenching of the excited S1 state by internal conversion (IC) to the ground state was observed. In the para compounds, IC, presumably promoted by the internal twisting, arises in <1 ps. A similar process takes place in the meta compounds in nonaqueous solvents but with notably slower kinetics. In aqueous solutions, the meta compounds undergo ultrafast intermolecular excited-state proton transfer that competes with isomerization.</description><identifier>ISSN: 1520-6106</identifier><identifier>EISSN: 1520-5207</identifier><identifier>DOI: 10.1021/jp077707t</identifier><identifier>PMID: 18269276</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Fluorescent Dyes - chemical synthesis ; Fluorescent Dyes - chemistry ; Green Fluorescent Proteins - chemistry ; Hydrogen-Ion Concentration ; Imidazolines - chemistry ; Isomerism ; Models, Chemical ; Solvents - chemistry ; Spectrometry, Fluorescence ; Spectrophotometry ; Spectrophotometry, Ultraviolet</subject><ispartof>The journal of physical chemistry. 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In aqueous solutions, the meta compounds undergo ultrafast intermolecular excited-state proton transfer that competes with isomerization.</description><subject>Fluorescent Dyes - chemical synthesis</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>Imidazolines - chemistry</subject><subject>Isomerism</subject><subject>Models, Chemical</subject><subject>Solvents - chemistry</subject><subject>Spectrometry, Fluorescence</subject><subject>Spectrophotometry</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>1520-6106</issn><issn>1520-5207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE9PwzAMxSMEgjE48AVQLiBxKCTtmrRHNMYfacAkBtfIbVytW9uMJJXg2xPYBBcOli29n57tR8gJZ5ecxfxquWZSSib9DhnwNGZRKLm7nQVn4oAcOrdkLE7jTOyTA57FIo-lGJDVI3qg0Gk6Awt0UlVYekfrjvoF0tfGW6jAeTr5KGuPOnrx4JHefHbQ1qWjpvrh7ixiR2-b3lh0JXaezqzxGFzGC2tas158C0dkr4LG4fG2D8nr7WQ-vo-mz3cP4-tpBCMufSRy5GmOgBnjSVFpXfBUaJaEPwoA0OENUWCBWTbiGhBZNipKloPOcswrFMmQnG9819a89-i8autwVdNAh6Z3SrJkFCcsCeDFBiytcc5ipda2bsF-Ks7Ud7LqN9nAnm5N-6JF_UduowxAtAFq5_HjVwe7UkImMlXz2YvK5vw-fRtL9RT4sw0PpVNL09suZPLP4i-69JAZ</recordid><startdate>20080306</startdate><enddate>20080306</enddate><creator>Solntsev, Kyril M</creator><creator>Poizat, Olivier</creator><creator>Dong, Jian</creator><creator>Rehault, Julien</creator><creator>Lou, Yongbing</creator><creator>Burda, Clemens</creator><creator>Tolbert, Laren M</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080306</creationdate><title>Meta and Para Effects in the Ultrafast Excited-State Dynamics of the Green Fluorescent Protein Chromophores</title><author>Solntsev, Kyril M ; Poizat, Olivier ; Dong, Jian ; Rehault, Julien ; Lou, Yongbing ; Burda, Clemens ; Tolbert, Laren M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a417t-69e159eae8013bfddb156d03106baaad0026bebe8841daee084bc09ad89e9fe63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Fluorescent Dyes - chemical synthesis</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Green Fluorescent Proteins - chemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>Imidazolines - chemistry</topic><topic>Isomerism</topic><topic>Models, Chemical</topic><topic>Solvents - chemistry</topic><topic>Spectrometry, Fluorescence</topic><topic>Spectrophotometry</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Solntsev, Kyril M</creatorcontrib><creatorcontrib>Poizat, Olivier</creatorcontrib><creatorcontrib>Dong, Jian</creatorcontrib><creatorcontrib>Rehault, Julien</creatorcontrib><creatorcontrib>Lou, Yongbing</creatorcontrib><creatorcontrib>Burda, Clemens</creatorcontrib><creatorcontrib>Tolbert, Laren M</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of physical chemistry. B</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Solntsev, Kyril M</au><au>Poizat, Olivier</au><au>Dong, Jian</au><au>Rehault, Julien</au><au>Lou, Yongbing</au><au>Burda, Clemens</au><au>Tolbert, Laren M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Meta and Para Effects in the Ultrafast Excited-State Dynamics of the Green Fluorescent Protein Chromophores</atitle><jtitle>The journal of physical chemistry. B</jtitle><addtitle>J. Phys. Chem. B</addtitle><date>2008-03-06</date><risdate>2008</risdate><volume>112</volume><issue>9</issue><spage>2700</spage><epage>2711</epage><pages>2700-2711</pages><issn>1520-6106</issn><eissn>1520-5207</eissn><abstract>Femtosecond transient absorption and fluorescence upconversion experiments have been performed to investigate the photoinduced dynamics of the meta isomer of the green fluorescent protein chromophore, m-HBDI, and its O-methylated derivative, m-MeOBDI, in various solvent mixtures at neutral, acidic, and basic pH. The para isomer, p-HBDI, and its O- and N-methylated derivatives, p-MeOBDI and p-HBDIMe+, were also studied for comparison. In all cases, fast quenching of the excited S1 state by internal conversion (IC) to the ground state was observed. In the para compounds, IC, presumably promoted by the internal twisting, arises in <1 ps. A similar process takes place in the meta compounds in nonaqueous solvents but with notably slower kinetics. In aqueous solutions, the meta compounds undergo ultrafast intermolecular excited-state proton transfer that competes with isomerization.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>18269276</pmid><doi>10.1021/jp077707t</doi><tpages>12</tpages></addata></record> |
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| subjects | Fluorescent Dyes - chemical synthesis Fluorescent Dyes - chemistry Green Fluorescent Proteins - chemistry Hydrogen-Ion Concentration Imidazolines - chemistry Isomerism Models, Chemical Solvents - chemistry Spectrometry, Fluorescence Spectrophotometry Spectrophotometry, Ultraviolet |
| title | Meta and Para Effects in the Ultrafast Excited-State Dynamics of the Green Fluorescent Protein Chromophores |
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