The alveolar epithelial type I-like cell line as an adequate model for leukocyte migration studies in vitro
The lung is unique as leukocytes not only migrate into the bronchoalveolar space but also return to the parenchyma and then via the lymphatics to the draining lymph node. The aim of this study was to investigate the migration of leukocytes via an epithelial monolayer in a Transwell system against a...
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| Veröffentlicht in: | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2007-04, Vol.58 (5), p.277-283 |
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| container_title | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie |
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| creator | Lührmann, Anke Bargsten, Gerhard Kuzu, Meryem Koslowski, Roland Pabst, Reinhard Tschernig, Thomas |
| description | The lung is unique as leukocytes not only migrate into the bronchoalveolar space but also return to the parenchyma and then via the lymphatics to the draining lymph node.
The aim of this study was to investigate the migration of leukocytes via an epithelial monolayer in a Transwell system against a chemokine gradient. Rat type I-like R3/1 alveolar epithelial cells were cultivated on a Transwell polyester membrane (pore diameter 3
μm) for 3 days until a monolayer was formed. The tightness of the monolayer was determined by transepithelial transport of horseradish peroxidase. Isolated human and rat peripheral blood mononuclear cells (PBMC) were placed in the upper chamber, and different concentrations of monocyte chemotactic protein-1 (MCP-1) in the lower chamber. The transmigration of PBMC was quantified and investigated by light and transmission electron microscopy. PBMC migrated through the epithelial cell barrier intercellularly as well as transcellularly. The migration of PBMC against the MCP-1 gradient was dose dependent. The results indicate that this model could help in the study of key events involved in chemokine-induced cell migration from the airways into tissue. |
| doi_str_mv | 10.1016/j.etp.2006.09.002 |
| format | Article |
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The aim of this study was to investigate the migration of leukocytes via an epithelial monolayer in a Transwell system against a chemokine gradient. Rat type I-like R3/1 alveolar epithelial cells were cultivated on a Transwell polyester membrane (pore diameter 3
μm) for 3 days until a monolayer was formed. The tightness of the monolayer was determined by transepithelial transport of horseradish peroxidase. Isolated human and rat peripheral blood mononuclear cells (PBMC) were placed in the upper chamber, and different concentrations of monocyte chemotactic protein-1 (MCP-1) in the lower chamber. The transmigration of PBMC was quantified and investigated by light and transmission electron microscopy. PBMC migrated through the epithelial cell barrier intercellularly as well as transcellularly. The migration of PBMC against the MCP-1 gradient was dose dependent. The results indicate that this model could help in the study of key events involved in chemokine-induced cell migration from the airways into tissue.</description><identifier>ISSN: 0940-2993</identifier><identifier>EISSN: 1618-1433</identifier><identifier>DOI: 10.1016/j.etp.2006.09.002</identifier><identifier>PMID: 17145177</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Animals ; Biological and medical sciences ; Chemokine CCL2 - pharmacology ; Chemotaxis, Leukocyte - drug effects ; Chemotaxis, Leukocyte - physiology ; Epithelial Cells - cytology ; Epithelial Cells - drug effects ; Epithelial Cells - ultrastructure ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Leukocytes - cytology ; Leukocytes - drug effects ; Leukocytes - physiology ; Leukocytes - ultrastructure ; Male ; MCP-1 ; Medical sciences ; Microscopy, Electron, Transmission ; Models, Biological ; Monolayer ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Pulmonary Alveoli - cytology ; Pulmonary Alveoli - drug effects ; Pulmonary Alveoli - ultrastructure ; Rat cell line ; Rats ; Rats, Inbred Lew ; Rats, Wistar ; Recombinant Proteins - pharmacology ; Transwell system</subject><ispartof>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2007-04, Vol.58 (5), p.277-283</ispartof><rights>2006 Elsevier GmbH</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c412t-549e067d34140f8b73afe24f04c2e8da053344fe10e22b2384e2e6cf4ca4c6e93</citedby><cites>FETCH-LOGICAL-c412t-549e067d34140f8b73afe24f04c2e8da053344fe10e22b2384e2e6cf4ca4c6e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.etp.2006.09.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18717479$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17145177$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lührmann, Anke</creatorcontrib><creatorcontrib>Bargsten, Gerhard</creatorcontrib><creatorcontrib>Kuzu, Meryem</creatorcontrib><creatorcontrib>Koslowski, Roland</creatorcontrib><creatorcontrib>Pabst, Reinhard</creatorcontrib><creatorcontrib>Tschernig, Thomas</creatorcontrib><title>The alveolar epithelial type I-like cell line as an adequate model for leukocyte migration studies in vitro</title><title>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</title><addtitle>Exp Toxicol Pathol</addtitle><description>The lung is unique as leukocytes not only migrate into the bronchoalveolar space but also return to the parenchyma and then via the lymphatics to the draining lymph node.
The aim of this study was to investigate the migration of leukocytes via an epithelial monolayer in a Transwell system against a chemokine gradient. Rat type I-like R3/1 alveolar epithelial cells were cultivated on a Transwell polyester membrane (pore diameter 3
μm) for 3 days until a monolayer was formed. The tightness of the monolayer was determined by transepithelial transport of horseradish peroxidase. Isolated human and rat peripheral blood mononuclear cells (PBMC) were placed in the upper chamber, and different concentrations of monocyte chemotactic protein-1 (MCP-1) in the lower chamber. The transmigration of PBMC was quantified and investigated by light and transmission electron microscopy. PBMC migrated through the epithelial cell barrier intercellularly as well as transcellularly. The migration of PBMC against the MCP-1 gradient was dose dependent. The results indicate that this model could help in the study of key events involved in chemokine-induced cell migration from the airways into tissue.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Chemokine CCL2 - pharmacology</subject><subject>Chemotaxis, Leukocyte - drug effects</subject><subject>Chemotaxis, Leukocyte - physiology</subject><subject>Epithelial Cells - cytology</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - ultrastructure</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Leukocytes - cytology</subject><subject>Leukocytes - drug effects</subject><subject>Leukocytes - physiology</subject><subject>Leukocytes - ultrastructure</subject><subject>Male</subject><subject>MCP-1</subject><subject>Medical sciences</subject><subject>Microscopy, Electron, Transmission</subject><subject>Models, Biological</subject><subject>Monolayer</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Pulmonary Alveoli - cytology</subject><subject>Pulmonary Alveoli - drug effects</subject><subject>Pulmonary Alveoli - ultrastructure</subject><subject>Rat cell line</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Rats, Wistar</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Transwell system</subject><issn>0940-2993</issn><issn>1618-1433</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9r3DAQxUVpaTZpP0AvRZf25u2MpLVseiqhfwKBXtKz0MrjRrtay5Hkhf32tdmF3JrTwPB7j5n3GPuAsEbA-stuTWVcC4B6De0aQLxiK6yxqVBJ-ZqtoFVQibaVV-w6590MQLvBt-wKNaoNar1i-4dH4jYcKQabOI2-PFLwNvByGonfVcHviTsKgQc_zGTmduC2o6fJFuKH2FHgfUw80LSP7rTs_N9ki48Dz2XqPGXuB370JcV37E1vQ6b3l3nD_vz4_nD7q7r__fPu9tt95RSKUm1US1DrTipU0DdbLW1PQvWgnKCms7CRUqmeEEiIrZCNIkG165WzytXUyhv2-ew7pvg0US7m4PPygx0oTtlokEqA0C-CAqEGhTiDeAZdijkn6s2Y_MGmk0EwSxVmZ-YqzFKFgdbMSc-ajxfzaXug7llxyX4GPl0Am50NfbKD8_mZazRqpZd3vp45mjM7ekomO0-Do84ncsV00f_njH_vIKbO</recordid><startdate>20070401</startdate><enddate>20070401</enddate><creator>Lührmann, Anke</creator><creator>Bargsten, Gerhard</creator><creator>Kuzu, Meryem</creator><creator>Koslowski, Roland</creator><creator>Pabst, Reinhard</creator><creator>Tschernig, Thomas</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7T5</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20070401</creationdate><title>The alveolar epithelial type I-like cell line as an adequate model for leukocyte migration studies in vitro</title><author>Lührmann, Anke ; Bargsten, Gerhard ; Kuzu, Meryem ; Koslowski, Roland ; Pabst, Reinhard ; Tschernig, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-549e067d34140f8b73afe24f04c2e8da053344fe10e22b2384e2e6cf4ca4c6e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Chemokine CCL2 - pharmacology</topic><topic>Chemotaxis, Leukocyte - drug effects</topic><topic>Chemotaxis, Leukocyte - physiology</topic><topic>Epithelial Cells - cytology</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - ultrastructure</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Leukocytes - cytology</topic><topic>Leukocytes - drug effects</topic><topic>Leukocytes - physiology</topic><topic>Leukocytes - ultrastructure</topic><topic>Male</topic><topic>MCP-1</topic><topic>Medical sciences</topic><topic>Microscopy, Electron, Transmission</topic><topic>Models, Biological</topic><topic>Monolayer</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Pulmonary Alveoli - cytology</topic><topic>Pulmonary Alveoli - drug effects</topic><topic>Pulmonary Alveoli - ultrastructure</topic><topic>Rat cell line</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Rats, Wistar</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Transwell system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lührmann, Anke</creatorcontrib><creatorcontrib>Bargsten, Gerhard</creatorcontrib><creatorcontrib>Kuzu, Meryem</creatorcontrib><creatorcontrib>Koslowski, Roland</creatorcontrib><creatorcontrib>Pabst, Reinhard</creatorcontrib><creatorcontrib>Tschernig, Thomas</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lührmann, Anke</au><au>Bargsten, Gerhard</au><au>Kuzu, Meryem</au><au>Koslowski, Roland</au><au>Pabst, Reinhard</au><au>Tschernig, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The alveolar epithelial type I-like cell line as an adequate model for leukocyte migration studies in vitro</atitle><jtitle>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</jtitle><addtitle>Exp Toxicol Pathol</addtitle><date>2007-04-01</date><risdate>2007</risdate><volume>58</volume><issue>5</issue><spage>277</spage><epage>283</epage><pages>277-283</pages><issn>0940-2993</issn><eissn>1618-1433</eissn><abstract>The lung is unique as leukocytes not only migrate into the bronchoalveolar space but also return to the parenchyma and then via the lymphatics to the draining lymph node.
The aim of this study was to investigate the migration of leukocytes via an epithelial monolayer in a Transwell system against a chemokine gradient. Rat type I-like R3/1 alveolar epithelial cells were cultivated on a Transwell polyester membrane (pore diameter 3
μm) for 3 days until a monolayer was formed. The tightness of the monolayer was determined by transepithelial transport of horseradish peroxidase. Isolated human and rat peripheral blood mononuclear cells (PBMC) were placed in the upper chamber, and different concentrations of monocyte chemotactic protein-1 (MCP-1) in the lower chamber. The transmigration of PBMC was quantified and investigated by light and transmission electron microscopy. PBMC migrated through the epithelial cell barrier intercellularly as well as transcellularly. The migration of PBMC against the MCP-1 gradient was dose dependent. The results indicate that this model could help in the study of key events involved in chemokine-induced cell migration from the airways into tissue.</abstract><cop>Jena</cop><pub>Elsevier GmbH</pub><pmid>17145177</pmid><doi>10.1016/j.etp.2006.09.002</doi><tpages>7</tpages></addata></record> |
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| ispartof | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2007-04, Vol.58 (5), p.277-283 |
| issn | 0940-2993 1618-1433 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Chemokine CCL2 - pharmacology Chemotaxis, Leukocyte - drug effects Chemotaxis, Leukocyte - physiology Epithelial Cells - cytology Epithelial Cells - drug effects Epithelial Cells - ultrastructure Humans Investigative techniques, diagnostic techniques (general aspects) Leukocytes - cytology Leukocytes - drug effects Leukocytes - physiology Leukocytes - ultrastructure Male MCP-1 Medical sciences Microscopy, Electron, Transmission Models, Biological Monolayer Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Pulmonary Alveoli - cytology Pulmonary Alveoli - drug effects Pulmonary Alveoli - ultrastructure Rat cell line Rats Rats, Inbred Lew Rats, Wistar Recombinant Proteins - pharmacology Transwell system |
| title | The alveolar epithelial type I-like cell line as an adequate model for leukocyte migration studies in vitro |
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