Eukaryotic expression and secretion of EGFP-labeled annexin A5

The Ca2+-dependent binding of annexin A5 to phosphatidylserine on cell surfaces is a reliable marker for apoptosis that is widely used in flow cytometry based apoptosis assays. In this approach, annexin A5 must be coupled to a fluorescent dye, but standard dyes such as fluorescein are photolabile, a...

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Veröffentlicht in:	Protein expression and purification 2008-04, Vol.58 (2), p.325-331
Hauptverfasser:	Stöcker, Michael, Pardo, Alessa, Hetzel, Christian, Reutelingsperger, Chris, Fischer, Rainer, Barth, Stefan
Format:	Artikel
Sprache:	eng
Schlagworte:	Annexin A5 Annexin A5 - biosynthesis Annexin A5 - secretion Apoptosis Apoptosis - drug effects Blotting, Western Cells, Cultured Cloning, Molecular EGFP Electrophoresis, Polyacrylamide Gel Eukaryotic expression Green Fluorescent Proteins - chemistry Humans Kidney Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - secretion Secretion U937 Cells
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