Prospective diagnostic efficiency of biopsy washing DNA GSTP1 island hypermethylation for detection of adenocarcinoma of the prostate
BACKGROUND The prospective diagnostic efficiency of quantitative glutathione S transferase (GSTP1) promoter hypermethylation analysis in biopsy washing samples has not been determined so far. METHODS Biopsies were obtained prospectively from 86 patients suspicious for prostate cancer (CaP). After is...
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| Veröffentlicht in: | The Prostate 2007-05, Vol.67 (7), p.757-763 |
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| container_title | The Prostate |
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| creator | Eilers, Tyark Machtens, Stefan Tezval, Hossein Blaue, Christoph Lichtinghagen, Ralf Hagemann, Jörn Jonas, Udo Serth, Jürgen |
| description | BACKGROUND
The prospective diagnostic efficiency of quantitative glutathione S transferase (GSTP1) promoter hypermethylation analysis in biopsy washing samples has not been determined so far.
METHODS
Biopsies were obtained prospectively from 86 patients suspicious for prostate cancer (CaP). After isolation of DNA from biopsy washings and bisulfite conversion methylated and unmethylated GSTP1 sequences were specifically quantitated by real‐time fluorescence PCR. Relative degrees of methylation were compared to results of histopathological examination.
RESULTS
Increased relative methylation was found for the CaP group (mean 28.1%) compared to biopsies without histological evidence for malignancy (5.2%; P |
| doi_str_mv | 10.1002/pros.20546 |
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The prospective diagnostic efficiency of quantitative glutathione S transferase (GSTP1) promoter hypermethylation analysis in biopsy washing samples has not been determined so far.
METHODS
Biopsies were obtained prospectively from 86 patients suspicious for prostate cancer (CaP). After isolation of DNA from biopsy washings and bisulfite conversion methylated and unmethylated GSTP1 sequences were specifically quantitated by real‐time fluorescence PCR. Relative degrees of methylation were compared to results of histopathological examination.
RESULTS
Increased relative methylation was found for the CaP group (mean 28.1%) compared to biopsies without histological evidence for malignancy (5.2%; P < 0.001). A sensitivity and specificity of 92% and 86% and positive and negative predictive values of 82% and 94% were obtained. Receiver operating characteristic (ROC) analysis demonstrated a value of 0.90 (95% CI 0.82–0.98) for the area under curve (AUC).
CONCLUSIONS
Biopsy washing DNA GSTP1 hypermethylation analysis demonstrates a high diagnostic efficacy which is comparable to the retrospective analysis of biopsy tissue specimens. Moreover it is compatible with routine biopsy examination thus permitting further prospective evaluation in CaP diagnosis. Prostate 67: 757–763, 2007. © 2007 Wiley‐Liss, Inc.</description><identifier>ISSN: 0270-4137</identifier><identifier>EISSN: 1097-0045</identifier><identifier>DOI: 10.1002/pros.20546</identifier><identifier>PMID: 17373715</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenocarcinoma - diagnosis ; Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Aged ; Biomarkers, Tumor ; biopsy washing ; Biopsy, Needle ; Cell Count ; diagnostic efficacy ; DNA Methylation ; DNA, Neoplasm - genetics ; DNA, Neoplasm - metabolism ; Gene Expression Regulation, Neoplastic ; Glutathione S-Transferase pi - genetics ; Glutathione S-Transferase pi - metabolism ; GSTP1 ; Humans ; hypermethylation ; Male ; Predictive Value of Tests ; Promoter Regions, Genetic ; Prospective Studies ; Prostate - metabolism ; Prostate - pathology ; prostate cancer ; Prostatic Neoplasms - diagnosis ; Prostatic Neoplasms - genetics ; Prostatic Neoplasms - metabolism ; Retrospective Studies ; Sensitivity and Specificity</subject><ispartof>The Prostate, 2007-05, Vol.67 (7), p.757-763</ispartof><rights>Copyright © 2007 Wiley‐Liss, Inc.</rights><rights>(c) 2007 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2956-e36f6dcaef9057eccdc038a951291d1873878ee6e0f40a89d66f59e59705347f3</citedby><cites>FETCH-LOGICAL-c2956-e36f6dcaef9057eccdc038a951291d1873878ee6e0f40a89d66f59e59705347f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpros.20546$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpros.20546$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17373715$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Eilers, Tyark</creatorcontrib><creatorcontrib>Machtens, Stefan</creatorcontrib><creatorcontrib>Tezval, Hossein</creatorcontrib><creatorcontrib>Blaue, Christoph</creatorcontrib><creatorcontrib>Lichtinghagen, Ralf</creatorcontrib><creatorcontrib>Hagemann, Jörn</creatorcontrib><creatorcontrib>Jonas, Udo</creatorcontrib><creatorcontrib>Serth, Jürgen</creatorcontrib><title>Prospective diagnostic efficiency of biopsy washing DNA GSTP1 island hypermethylation for detection of adenocarcinoma of the prostate</title><title>The Prostate</title><addtitle>Prostate</addtitle><description>BACKGROUND
The prospective diagnostic efficiency of quantitative glutathione S transferase (GSTP1) promoter hypermethylation analysis in biopsy washing samples has not been determined so far.
METHODS
Biopsies were obtained prospectively from 86 patients suspicious for prostate cancer (CaP). After isolation of DNA from biopsy washings and bisulfite conversion methylated and unmethylated GSTP1 sequences were specifically quantitated by real‐time fluorescence PCR. Relative degrees of methylation were compared to results of histopathological examination.
RESULTS
Increased relative methylation was found for the CaP group (mean 28.1%) compared to biopsies without histological evidence for malignancy (5.2%; P < 0.001). A sensitivity and specificity of 92% and 86% and positive and negative predictive values of 82% and 94% were obtained. Receiver operating characteristic (ROC) analysis demonstrated a value of 0.90 (95% CI 0.82–0.98) for the area under curve (AUC).
CONCLUSIONS
Biopsy washing DNA GSTP1 hypermethylation analysis demonstrates a high diagnostic efficacy which is comparable to the retrospective analysis of biopsy tissue specimens. Moreover it is compatible with routine biopsy examination thus permitting further prospective evaluation in CaP diagnosis. Prostate 67: 757–763, 2007. © 2007 Wiley‐Liss, Inc.</description><subject>Adenocarcinoma - diagnosis</subject><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Aged</subject><subject>Biomarkers, Tumor</subject><subject>biopsy washing</subject><subject>Biopsy, Needle</subject><subject>Cell Count</subject><subject>diagnostic efficacy</subject><subject>DNA Methylation</subject><subject>DNA, Neoplasm - genetics</subject><subject>DNA, Neoplasm - metabolism</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Glutathione S-Transferase pi - genetics</subject><subject>Glutathione S-Transferase pi - metabolism</subject><subject>GSTP1</subject><subject>Humans</subject><subject>hypermethylation</subject><subject>Male</subject><subject>Predictive Value of Tests</subject><subject>Promoter Regions, Genetic</subject><subject>Prospective Studies</subject><subject>Prostate - metabolism</subject><subject>Prostate - pathology</subject><subject>prostate cancer</subject><subject>Prostatic Neoplasms - diagnosis</subject><subject>Prostatic Neoplasms - genetics</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Retrospective Studies</subject><subject>Sensitivity and Specificity</subject><issn>0270-4137</issn><issn>1097-0045</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1u1DAURi0EokNhwwMgr1ggpVzHcRwvqymdIg3tqC1iabnOdceQv8YeSh6A98ZhBtghLyxb5zv-fAl5zeCEAeTvh7EPJzmIonxCFgyUzAAK8ZQsIJeQFYzLI_IihK8ACYf8OTlikqfFxIL83KTwgDb670hrb-67PkRvKTrnrcfOTrR39M73Q5joowlb393Ts8tTurq53TDqQ2O6mm6nAccW43ZqTPR9R10_0hrj7E2nZDA1dr01o_Vd35r5Jm6Rzs2jifiSPHOmCfjqsB-Tz-cfbpcX2fpq9XF5us5srkSZIS9dWVuDToGQaG1tgVdGCZYrVrNK8kpWiCWCK8BUqi5LJxQKJUHwQjp-TN7uvenhhx2GqFsfLDbpE9jvgpbAeVUolcB3e9CmhmFEp4fRt2acNAM9D13P1fXvoSf4zcG6u2ux_oceppwAtgcefYPTf1R6c31180ea7TM-RPzxN2PGb7pMVqG_XK70-fX6gp19KvSS_wLOr55m</recordid><startdate>20070515</startdate><enddate>20070515</enddate><creator>Eilers, Tyark</creator><creator>Machtens, Stefan</creator><creator>Tezval, Hossein</creator><creator>Blaue, Christoph</creator><creator>Lichtinghagen, Ralf</creator><creator>Hagemann, Jörn</creator><creator>Jonas, Udo</creator><creator>Serth, Jürgen</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20070515</creationdate><title>Prospective diagnostic efficiency of biopsy washing DNA GSTP1 island hypermethylation for detection of adenocarcinoma of the prostate</title><author>Eilers, Tyark ; Machtens, Stefan ; Tezval, Hossein ; Blaue, Christoph ; Lichtinghagen, Ralf ; Hagemann, Jörn ; Jonas, Udo ; Serth, Jürgen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2956-e36f6dcaef9057eccdc038a951291d1873878ee6e0f40a89d66f59e59705347f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Adenocarcinoma - diagnosis</topic><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Aged</topic><topic>Biomarkers, Tumor</topic><topic>biopsy washing</topic><topic>Biopsy, Needle</topic><topic>Cell Count</topic><topic>diagnostic efficacy</topic><topic>DNA Methylation</topic><topic>DNA, Neoplasm - genetics</topic><topic>DNA, Neoplasm - metabolism</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Glutathione S-Transferase pi - genetics</topic><topic>Glutathione S-Transferase pi - metabolism</topic><topic>GSTP1</topic><topic>Humans</topic><topic>hypermethylation</topic><topic>Male</topic><topic>Predictive Value of Tests</topic><topic>Promoter Regions, Genetic</topic><topic>Prospective Studies</topic><topic>Prostate - metabolism</topic><topic>Prostate - pathology</topic><topic>prostate cancer</topic><topic>Prostatic Neoplasms - diagnosis</topic><topic>Prostatic Neoplasms - genetics</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Retrospective Studies</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Eilers, Tyark</creatorcontrib><creatorcontrib>Machtens, Stefan</creatorcontrib><creatorcontrib>Tezval, Hossein</creatorcontrib><creatorcontrib>Blaue, Christoph</creatorcontrib><creatorcontrib>Lichtinghagen, Ralf</creatorcontrib><creatorcontrib>Hagemann, Jörn</creatorcontrib><creatorcontrib>Jonas, Udo</creatorcontrib><creatorcontrib>Serth, Jürgen</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Prostate</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Eilers, Tyark</au><au>Machtens, Stefan</au><au>Tezval, Hossein</au><au>Blaue, Christoph</au><au>Lichtinghagen, Ralf</au><au>Hagemann, Jörn</au><au>Jonas, Udo</au><au>Serth, Jürgen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prospective diagnostic efficiency of biopsy washing DNA GSTP1 island hypermethylation for detection of adenocarcinoma of the prostate</atitle><jtitle>The Prostate</jtitle><addtitle>Prostate</addtitle><date>2007-05-15</date><risdate>2007</risdate><volume>67</volume><issue>7</issue><spage>757</spage><epage>763</epage><pages>757-763</pages><issn>0270-4137</issn><eissn>1097-0045</eissn><abstract>BACKGROUND
The prospective diagnostic efficiency of quantitative glutathione S transferase (GSTP1) promoter hypermethylation analysis in biopsy washing samples has not been determined so far.
METHODS
Biopsies were obtained prospectively from 86 patients suspicious for prostate cancer (CaP). After isolation of DNA from biopsy washings and bisulfite conversion methylated and unmethylated GSTP1 sequences were specifically quantitated by real‐time fluorescence PCR. Relative degrees of methylation were compared to results of histopathological examination.
RESULTS
Increased relative methylation was found for the CaP group (mean 28.1%) compared to biopsies without histological evidence for malignancy (5.2%; P < 0.001). A sensitivity and specificity of 92% and 86% and positive and negative predictive values of 82% and 94% were obtained. Receiver operating characteristic (ROC) analysis demonstrated a value of 0.90 (95% CI 0.82–0.98) for the area under curve (AUC).
CONCLUSIONS
Biopsy washing DNA GSTP1 hypermethylation analysis demonstrates a high diagnostic efficacy which is comparable to the retrospective analysis of biopsy tissue specimens. Moreover it is compatible with routine biopsy examination thus permitting further prospective evaluation in CaP diagnosis. Prostate 67: 757–763, 2007. © 2007 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17373715</pmid><doi>10.1002/pros.20546</doi><tpages>7</tpages></addata></record> |
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| subjects | Adenocarcinoma - diagnosis Adenocarcinoma - genetics Adenocarcinoma - metabolism Aged Biomarkers, Tumor biopsy washing Biopsy, Needle Cell Count diagnostic efficacy DNA Methylation DNA, Neoplasm - genetics DNA, Neoplasm - metabolism Gene Expression Regulation, Neoplastic Glutathione S-Transferase pi - genetics Glutathione S-Transferase pi - metabolism GSTP1 Humans hypermethylation Male Predictive Value of Tests Promoter Regions, Genetic Prospective Studies Prostate - metabolism Prostate - pathology prostate cancer Prostatic Neoplasms - diagnosis Prostatic Neoplasms - genetics Prostatic Neoplasms - metabolism Retrospective Studies Sensitivity and Specificity |
| title | Prospective diagnostic efficiency of biopsy washing DNA GSTP1 island hypermethylation for detection of adenocarcinoma of the prostate |
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