guanylyl cyclase-like gene is associated with Gibberella ear rot resistance in maize (Zea mays L.)
Gibberella ear rot, caused by the fungal pathogen Fusarium graminearum Schwabe, is a serious disease of maize (Zea mays L.) grown in northern climates. The infected maize grain contains toxins that are very harmful to livestock and humans. A maize gene that encodes a putative 267-amino acid guanylyl...
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| Veröffentlicht in: | Theoretical and applied genetics 2008-02, Vol.116 (4), p.465-479 |
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| creator | Yuan, J Ali, M. Liakat Taylor, J Liu, J Sun, G Liu, W Masilimany, P Gulati-Sakhuja, A Pauls, K. P |
| description | Gibberella ear rot, caused by the fungal pathogen Fusarium graminearum Schwabe, is a serious disease of maize (Zea mays L.) grown in northern climates. The infected maize grain contains toxins that are very harmful to livestock and humans. A maize gene that encodes a putative 267-amino acid guanylyl cyclase-like protein (ZmGC1) was characterized and shown to be associated with resistance to this disease. The putative ZmGC1 amino acid sequence is 53% identical and 65% similar to AtGC1, an Arabidopsis guanylyl cyclase. The Zmgc1 coding sequence is nearly identical in a Gibberella ear rot-resistant line (CO387) and a susceptible line (CG62) but several nucleotide sequence differences were observed in the UTRs and introns of the two alleles. Using a 463 bp probe derived from the CG62 allele of Zmgc1 and a recombinant inbred (RI) mapping population developed from a CG62 x CO387 cross, six Zmgc1 restriction fragment length polymorphism (RFLP) fragments (ER1_1, ER1_2, ER1_3, ER1_4, ER1_5, and ER5_1) were mapped on maize chromosomes 2, 3, 7, and 8. Markers ER1_1 and ER5_1 on chromosomes 7 and 8, respectively, were significantly associated with Gibberella ear rot resistance, each in three different environments. The amount of Zmgc1 transcript in ear tissues increased more quickly and to a greater extent in the resistant genotype compared to the susceptible genotype after inoculation with F. graminearum. Zmgc1 is the first guanylyl cyclase gene characterized in maize and the first gene found to be associated with Gibberella ear rot resistance in this plant. |
| doi_str_mv | 10.1007/s00122-007-0683-1 |
| format | Article |
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Liakat ; Taylor, J ; Liu, J ; Sun, G ; Liu, W ; Masilimany, P ; Gulati-Sakhuja, A ; Pauls, K. P</creator><creatorcontrib>Yuan, J ; Ali, M. Liakat ; Taylor, J ; Liu, J ; Sun, G ; Liu, W ; Masilimany, P ; Gulati-Sakhuja, A ; Pauls, K. P</creatorcontrib><description>Gibberella ear rot, caused by the fungal pathogen Fusarium graminearum Schwabe, is a serious disease of maize (Zea mays L.) grown in northern climates. The infected maize grain contains toxins that are very harmful to livestock and humans. A maize gene that encodes a putative 267-amino acid guanylyl cyclase-like protein (ZmGC1) was characterized and shown to be associated with resistance to this disease. The putative ZmGC1 amino acid sequence is 53% identical and 65% similar to AtGC1, an Arabidopsis guanylyl cyclase. The Zmgc1 coding sequence is nearly identical in a Gibberella ear rot-resistant line (CO387) and a susceptible line (CG62) but several nucleotide sequence differences were observed in the UTRs and introns of the two alleles. Using a 463 bp probe derived from the CG62 allele of Zmgc1 and a recombinant inbred (RI) mapping population developed from a CG62 x CO387 cross, six Zmgc1 restriction fragment length polymorphism (RFLP) fragments (ER1_1, ER1_2, ER1_3, ER1_4, ER1_5, and ER5_1) were mapped on maize chromosomes 2, 3, 7, and 8. Markers ER1_1 and ER5_1 on chromosomes 7 and 8, respectively, were significantly associated with Gibberella ear rot resistance, each in three different environments. The amount of Zmgc1 transcript in ear tissues increased more quickly and to a greater extent in the resistant genotype compared to the susceptible genotype after inoculation with F. graminearum. Zmgc1 is the first guanylyl cyclase gene characterized in maize and the first gene found to be associated with Gibberella ear rot resistance in this plant.</description><identifier>ISSN: 0040-5752</identifier><identifier>EISSN: 1432-2242</identifier><identifier>DOI: 10.1007/s00122-007-0683-1</identifier><identifier>PMID: 18074115</identifier><identifier>CODEN: THAGA6</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Agriculture ; Amino Acid Sequence ; Arabidopsis ; Base Sequence ; Biochemistry ; Biological and medical sciences ; Biomedical and Life Sciences ; Biotechnology ; Chromosome Mapping ; Chromosomes ; Classical genetics, quantitative genetics, hybrids ; DNA Primers ; DNA, Plant - genetics ; Fundamental and applied biological sciences. Psychology ; Fusarium graminearum ; Genes ; Genetic Markers - genetics ; Genetics of eukaryotes. Biological and molecular evolution ; Genotype ; Gibberella - genetics ; Gibberella - growth & development ; Gibberella - pathogenicity ; Guanylate Cyclase - genetics ; Guanylate Cyclase - metabolism ; Life Sciences ; Molecular Sequence Data ; Original Paper ; Pathogenesis ; Pathogens ; Phylogeny ; Plant Biochemistry ; Plant Breeding/Biotechnology ; Plant Diseases - genetics ; Plant Diseases - microbiology ; Plant Genetics and Genomics ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Pteridophyta, spermatophyta ; Quantitative Trait Loci ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Seeds - microbiology ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; Signal transduction ; Vegetals ; Zea mays ; Zea mays - genetics ; Zea mays - growth & development ; Zea mays - microbiology</subject><ispartof>Theoretical and applied genetics, 2008-02, Vol.116 (4), p.465-479</ispartof><rights>Springer-Verlag 2007</rights><rights>2008 INIST-CNRS</rights><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c454t-36cdead5f8d854a904620d10319f6f61b05f1e287148aefc90fc5ed49e1ff2fe3</citedby><cites>FETCH-LOGICAL-c454t-36cdead5f8d854a904620d10319f6f61b05f1e287148aefc90fc5ed49e1ff2fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00122-007-0683-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00122-007-0683-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27928,27929,41492,42561,51323</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20126514$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18074115$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yuan, J</creatorcontrib><creatorcontrib>Ali, M. Liakat</creatorcontrib><creatorcontrib>Taylor, J</creatorcontrib><creatorcontrib>Liu, J</creatorcontrib><creatorcontrib>Sun, G</creatorcontrib><creatorcontrib>Liu, W</creatorcontrib><creatorcontrib>Masilimany, P</creatorcontrib><creatorcontrib>Gulati-Sakhuja, A</creatorcontrib><creatorcontrib>Pauls, K. P</creatorcontrib><title>guanylyl cyclase-like gene is associated with Gibberella ear rot resistance in maize (Zea mays L.)</title><title>Theoretical and applied genetics</title><addtitle>Theor Appl Genet</addtitle><addtitle>Theor Appl Genet</addtitle><description>Gibberella ear rot, caused by the fungal pathogen Fusarium graminearum Schwabe, is a serious disease of maize (Zea mays L.) grown in northern climates. The infected maize grain contains toxins that are very harmful to livestock and humans. A maize gene that encodes a putative 267-amino acid guanylyl cyclase-like protein (ZmGC1) was characterized and shown to be associated with resistance to this disease. The putative ZmGC1 amino acid sequence is 53% identical and 65% similar to AtGC1, an Arabidopsis guanylyl cyclase. The Zmgc1 coding sequence is nearly identical in a Gibberella ear rot-resistant line (CO387) and a susceptible line (CG62) but several nucleotide sequence differences were observed in the UTRs and introns of the two alleles. Using a 463 bp probe derived from the CG62 allele of Zmgc1 and a recombinant inbred (RI) mapping population developed from a CG62 x CO387 cross, six Zmgc1 restriction fragment length polymorphism (RFLP) fragments (ER1_1, ER1_2, ER1_3, ER1_4, ER1_5, and ER5_1) were mapped on maize chromosomes 2, 3, 7, and 8. Markers ER1_1 and ER5_1 on chromosomes 7 and 8, respectively, were significantly associated with Gibberella ear rot resistance, each in three different environments. The amount of Zmgc1 transcript in ear tissues increased more quickly and to a greater extent in the resistant genotype compared to the susceptible genotype after inoculation with F. graminearum. Zmgc1 is the first guanylyl cyclase gene characterized in maize and the first gene found to be associated with Gibberella ear rot resistance in this plant.</description><subject>Agriculture</subject><subject>Amino Acid Sequence</subject><subject>Arabidopsis</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Chromosome Mapping</subject><subject>Chromosomes</subject><subject>Classical genetics, quantitative genetics, hybrids</subject><subject>DNA Primers</subject><subject>DNA, Plant - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fusarium graminearum</subject><subject>Genes</subject><subject>Genetic Markers - genetics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genotype</subject><subject>Gibberella - genetics</subject><subject>Gibberella - growth & development</subject><subject>Gibberella - pathogenicity</subject><subject>Guanylate Cyclase - genetics</subject><subject>Guanylate Cyclase - metabolism</subject><subject>Life Sciences</subject><subject>Molecular Sequence Data</subject><subject>Original Paper</subject><subject>Pathogenesis</subject><subject>Pathogens</subject><subject>Phylogeny</subject><subject>Plant Biochemistry</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Genetics and Genomics</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Pteridophyta, spermatophyta</subject><subject>Quantitative Trait Loci</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Seeds - microbiology</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Signal transduction</subject><subject>Vegetals</subject><subject>Zea mays</subject><subject>Zea mays - genetics</subject><subject>Zea mays - growth & development</subject><subject>Zea mays - microbiology</subject><issn>0040-5752</issn><issn>1432-2242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkUGL1TAUhYMozvPpD3CjQVB00fEmTdp0KYOOwgMXOhs3IS-9eWbsa2dyW6T-elP6cMCFQiAX8p2TkxzGngo4FwD1WwIQUhZ5LKAyZSHusY1QpSykVPI-2wAoKHSt5Rl7RHQNAFJD-ZCdCQO1EkJv2P4wuX7u5o772XeOsOjiD-QH7JFH4o5o8NGN2PKfcfzOL-N-jwm7znF0iadh5Akp0uh6nwU9P7r4C_nrb-jyOBPfnb95zB4E1xE-Oe1bdvXh_deLj8Xu8-Wni3e7wiutxqKsfIuu1cG0RivXgKoktAJK0YQqVGIPOgiUphbKOAy-geA1tqpBEYIMWG7Zq9X3Jg23E9Joj5H8krXHYSJbQ1kqZfR_QdEYU-WVwRd_gdfDlPr8CCtB6brOaTIkVsingShhsDcpHl2arQC71GTXmuwyLjVZkTXPTsbT_ojtneLUSwZengBH3nUh5f-N9IeT2bDSueotkytH-ag_YLpL-K_bn6-i4AbrDikbX33JliWAqdRi-xsTbbHq</recordid><startdate>20080201</startdate><enddate>20080201</enddate><creator>Yuan, J</creator><creator>Ali, M. 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Liakat ; Taylor, J ; Liu, J ; Sun, G ; Liu, W ; Masilimany, P ; Gulati-Sakhuja, A ; Pauls, K. P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c454t-36cdead5f8d854a904620d10319f6f61b05f1e287148aefc90fc5ed49e1ff2fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Agriculture</topic><topic>Amino Acid Sequence</topic><topic>Arabidopsis</topic><topic>Base Sequence</topic><topic>Biochemistry</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Chromosome Mapping</topic><topic>Chromosomes</topic><topic>Classical genetics, quantitative genetics, hybrids</topic><topic>DNA Primers</topic><topic>DNA, Plant - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fusarium graminearum</topic><topic>Genes</topic><topic>Genetic Markers - genetics</topic><topic>Genetics of eukaryotes. 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Liakat</au><au>Taylor, J</au><au>Liu, J</au><au>Sun, G</au><au>Liu, W</au><au>Masilimany, P</au><au>Gulati-Sakhuja, A</au><au>Pauls, K. P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>guanylyl cyclase-like gene is associated with Gibberella ear rot resistance in maize (Zea mays L.)</atitle><jtitle>Theoretical and applied genetics</jtitle><stitle>Theor Appl Genet</stitle><addtitle>Theor Appl Genet</addtitle><date>2008-02-01</date><risdate>2008</risdate><volume>116</volume><issue>4</issue><spage>465</spage><epage>479</epage><pages>465-479</pages><issn>0040-5752</issn><eissn>1432-2242</eissn><coden>THAGA6</coden><abstract>Gibberella ear rot, caused by the fungal pathogen Fusarium graminearum Schwabe, is a serious disease of maize (Zea mays L.) grown in northern climates. The infected maize grain contains toxins that are very harmful to livestock and humans. A maize gene that encodes a putative 267-amino acid guanylyl cyclase-like protein (ZmGC1) was characterized and shown to be associated with resistance to this disease. The putative ZmGC1 amino acid sequence is 53% identical and 65% similar to AtGC1, an Arabidopsis guanylyl cyclase. The Zmgc1 coding sequence is nearly identical in a Gibberella ear rot-resistant line (CO387) and a susceptible line (CG62) but several nucleotide sequence differences were observed in the UTRs and introns of the two alleles. Using a 463 bp probe derived from the CG62 allele of Zmgc1 and a recombinant inbred (RI) mapping population developed from a CG62 x CO387 cross, six Zmgc1 restriction fragment length polymorphism (RFLP) fragments (ER1_1, ER1_2, ER1_3, ER1_4, ER1_5, and ER5_1) were mapped on maize chromosomes 2, 3, 7, and 8. Markers ER1_1 and ER5_1 on chromosomes 7 and 8, respectively, were significantly associated with Gibberella ear rot resistance, each in three different environments. The amount of Zmgc1 transcript in ear tissues increased more quickly and to a greater extent in the resistant genotype compared to the susceptible genotype after inoculation with F. graminearum. Zmgc1 is the first guanylyl cyclase gene characterized in maize and the first gene found to be associated with Gibberella ear rot resistance in this plant.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>18074115</pmid><doi>10.1007/s00122-007-0683-1</doi><tpages>15</tpages></addata></record> |
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| ispartof | Theoretical and applied genetics, 2008-02, Vol.116 (4), p.465-479 |
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| subjects | Agriculture Amino Acid Sequence Arabidopsis Base Sequence Biochemistry Biological and medical sciences Biomedical and Life Sciences Biotechnology Chromosome Mapping Chromosomes Classical genetics, quantitative genetics, hybrids DNA Primers DNA, Plant - genetics Fundamental and applied biological sciences. Psychology Fusarium graminearum Genes Genetic Markers - genetics Genetics of eukaryotes. Biological and molecular evolution Genotype Gibberella - genetics Gibberella - growth & development Gibberella - pathogenicity Guanylate Cyclase - genetics Guanylate Cyclase - metabolism Life Sciences Molecular Sequence Data Original Paper Pathogenesis Pathogens Phylogeny Plant Biochemistry Plant Breeding/Biotechnology Plant Diseases - genetics Plant Diseases - microbiology Plant Genetics and Genomics Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Pteridophyta, spermatophyta Quantitative Trait Loci RNA, Messenger - genetics RNA, Messenger - metabolism Seeds - microbiology Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Signal transduction Vegetals Zea mays Zea mays - genetics Zea mays - growth & development Zea mays - microbiology |
| title | guanylyl cyclase-like gene is associated with Gibberella ear rot resistance in maize (Zea mays L.) |
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