Brain neutral lipids mass is increased in α‐synuclein gene‐ablated mice
Because α‐synuclein (Snca) has a role in brain lipid metabolism, we determined the impact that Snca deletion had on whole brain lipid composition. We analysed masses of individual phospholipid (PL) classes and neutral lipid mass as well as PL acyl chain composition in brains from wild‐type and Snca‐...
Gespeichert in:
| Veröffentlicht in: | Journal of neurochemistry 2007-04, Vol.101 (1), p.132-141 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 141 |
|---|---|
| container_issue | 1 |
| container_start_page | 132 |
| container_title | Journal of neurochemistry |
| container_volume | 101 |
| creator | Barceló‐Coblijn, Gwendolyn Golovko, Mikhail Y. Weinhofer, Isabella Berger, Johannes Murphy, Eric J. |
| description | Because α‐synuclein (Snca) has a role in brain lipid metabolism, we determined the impact that Snca deletion had on whole brain lipid composition. We analysed masses of individual phospholipid (PL) classes and neutral lipid mass as well as PL acyl chain composition in brains from wild‐type and Snca‐/‐ mice. Although total brain PL mass was not altered, cardiolipin and phosphatidylglycerol mass decreased 16% and 27%, respectively, in Snca‐/‐ mice. In addition, no changes were observed in plasmalogen or polyphosphoinositide mass. In ethanolamine glycerophospholipids and phosphatidylserine, docosahexaenoic acid (22 : 6n‐3) was decreased 7%, while 16 : 0 was increased 1.1‐fold and 1.4‐fold, respectively. Surprisingly, brain cholesterol, cholesteryl ester, and triacylglycerol mass were increased 1.1‐fold, 1.6‐fold, and 1.4‐fold, respectively in Snca‐/‐ mice. In isolated myelin, cholesterol mass was also increased 1.3‐fold, but because there was also a net increase in myelin PL mass, the cholesterol to PL ratio was unaltered. No changes in the expression of cholesterogenic enzymes were observed, suggesting these did not account for the observed changes in cholesterol. These data extend our previous results in astrocytes and kinetic studies in vivo demonstrating a role for Snca in brain lipid metabolism and demonstrate a clear impact on brain neutral lipid metabolism. |
| doi_str_mv | 10.1111/j.1471-4159.2006.04348.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_70330675</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70330675</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3978-d8900b53841c03f8c10688715e8c8028ee9f262c9c0a72d5bd420cd12de717103</originalsourceid><addsrcrecordid>eNqNkEtu2zAQQIkiReMkvUKgTbOTOvxIpBZZNEabtDDaTbImaHIU0KBkh7TQeNcj9Cq9SA-Rk4SqjWYbggCHM2_IwSOkoFDRvD6uKiokLQWt24oBNBUILlT1-IbM_heOyAyAsZKDYMfkJKUVAG1EQ9-RYypZDY1qZmRxFY0figHHbTShCH7jXSp6k1Lh8x5sRJPQ5aj4--fp1--0G0YbMF_vccCcMMtgthnovcUz8rYzIeH7w3lK7r58vp3flIsf11_nnxal5a1UpVMtwLLmSlALvFOW5lmUpDUqq4ApxLZjDbOtBSOZq5dOMLCOMoeSSgr8lFzs393E9cOIaat7nyyGYAZcj0lL4BwaWWdQ7UEb1ylF7PQm-t7EnaagJ5N6pSdhehKmJ5P6n0n9mFvPD3-Myx7dS-NBXQY-HACTrAldNIP16YVTDXAGKnOXe-6nD7h79QD62_f5FPFnFl6QBw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70330675</pqid></control><display><type>article</type><title>Brain neutral lipids mass is increased in α‐synuclein gene‐ablated mice</title><source>MEDLINE</source><source>Wiley Journals</source><source>IngentaConnect Free/Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><source>Free Full-Text Journals in Chemistry</source><creator>Barceló‐Coblijn, Gwendolyn ; Golovko, Mikhail Y. ; Weinhofer, Isabella ; Berger, Johannes ; Murphy, Eric J.</creator><creatorcontrib>Barceló‐Coblijn, Gwendolyn ; Golovko, Mikhail Y. ; Weinhofer, Isabella ; Berger, Johannes ; Murphy, Eric J.</creatorcontrib><description>Because α‐synuclein (Snca) has a role in brain lipid metabolism, we determined the impact that Snca deletion had on whole brain lipid composition. We analysed masses of individual phospholipid (PL) classes and neutral lipid mass as well as PL acyl chain composition in brains from wild‐type and Snca‐/‐ mice. Although total brain PL mass was not altered, cardiolipin and phosphatidylglycerol mass decreased 16% and 27%, respectively, in Snca‐/‐ mice. In addition, no changes were observed in plasmalogen or polyphosphoinositide mass. In ethanolamine glycerophospholipids and phosphatidylserine, docosahexaenoic acid (22 : 6n‐3) was decreased 7%, while 16 : 0 was increased 1.1‐fold and 1.4‐fold, respectively. Surprisingly, brain cholesterol, cholesteryl ester, and triacylglycerol mass were increased 1.1‐fold, 1.6‐fold, and 1.4‐fold, respectively in Snca‐/‐ mice. In isolated myelin, cholesterol mass was also increased 1.3‐fold, but because there was also a net increase in myelin PL mass, the cholesterol to PL ratio was unaltered. No changes in the expression of cholesterogenic enzymes were observed, suggesting these did not account for the observed changes in cholesterol. These data extend our previous results in astrocytes and kinetic studies in vivo demonstrating a role for Snca in brain lipid metabolism and demonstrate a clear impact on brain neutral lipid metabolism.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.2006.04348.x</identifier><identifier>PMID: 17250686</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>alpha-Synuclein - genetics ; Animals ; Biochemistry and metabolism ; Biological and medical sciences ; Brain - metabolism ; Brain Chemistry - genetics ; Cardiolipins - metabolism ; Central nervous system ; cholesterol ; Cholesterol - metabolism ; cholesteryl esters ; Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Enzymologic - physiology ; Lipid Metabolism - genetics ; Medical sciences ; Membrane Lipids - metabolism ; Mice ; Mice, Knockout ; Myelin Sheath - metabolism ; Neurology ; Phosphatidylglycerols - metabolism ; Phospholipids - metabolism ; triacylglycerols ; Triglycerides - metabolism ; Vertebrates: nervous system and sense organs ; α‐synuclein</subject><ispartof>Journal of neurochemistry, 2007-04, Vol.101 (1), p.132-141</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3978-d8900b53841c03f8c10688715e8c8028ee9f262c9c0a72d5bd420cd12de717103</citedby><cites>FETCH-LOGICAL-c3978-d8900b53841c03f8c10688715e8c8028ee9f262c9c0a72d5bd420cd12de717103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.2006.04348.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.2006.04348.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18603208$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17250686$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barceló‐Coblijn, Gwendolyn</creatorcontrib><creatorcontrib>Golovko, Mikhail Y.</creatorcontrib><creatorcontrib>Weinhofer, Isabella</creatorcontrib><creatorcontrib>Berger, Johannes</creatorcontrib><creatorcontrib>Murphy, Eric J.</creatorcontrib><title>Brain neutral lipids mass is increased in α‐synuclein gene‐ablated mice</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>Because α‐synuclein (Snca) has a role in brain lipid metabolism, we determined the impact that Snca deletion had on whole brain lipid composition. We analysed masses of individual phospholipid (PL) classes and neutral lipid mass as well as PL acyl chain composition in brains from wild‐type and Snca‐/‐ mice. Although total brain PL mass was not altered, cardiolipin and phosphatidylglycerol mass decreased 16% and 27%, respectively, in Snca‐/‐ mice. In addition, no changes were observed in plasmalogen or polyphosphoinositide mass. In ethanolamine glycerophospholipids and phosphatidylserine, docosahexaenoic acid (22 : 6n‐3) was decreased 7%, while 16 : 0 was increased 1.1‐fold and 1.4‐fold, respectively. Surprisingly, brain cholesterol, cholesteryl ester, and triacylglycerol mass were increased 1.1‐fold, 1.6‐fold, and 1.4‐fold, respectively in Snca‐/‐ mice. In isolated myelin, cholesterol mass was also increased 1.3‐fold, but because there was also a net increase in myelin PL mass, the cholesterol to PL ratio was unaltered. No changes in the expression of cholesterogenic enzymes were observed, suggesting these did not account for the observed changes in cholesterol. These data extend our previous results in astrocytes and kinetic studies in vivo demonstrating a role for Snca in brain lipid metabolism and demonstrate a clear impact on brain neutral lipid metabolism.</description><subject>alpha-Synuclein - genetics</subject><subject>Animals</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain - metabolism</subject><subject>Brain Chemistry - genetics</subject><subject>Cardiolipins - metabolism</subject><subject>Central nervous system</subject><subject>cholesterol</subject><subject>Cholesterol - metabolism</subject><subject>cholesteryl esters</subject><subject>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Enzymologic - physiology</subject><subject>Lipid Metabolism - genetics</subject><subject>Medical sciences</subject><subject>Membrane Lipids - metabolism</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Myelin Sheath - metabolism</subject><subject>Neurology</subject><subject>Phosphatidylglycerols - metabolism</subject><subject>Phospholipids - metabolism</subject><subject>triacylglycerols</subject><subject>Triglycerides - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><subject>α‐synuclein</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtu2zAQQIkiReMkvUKgTbOTOvxIpBZZNEabtDDaTbImaHIU0KBkh7TQeNcj9Cq9SA-Rk4SqjWYbggCHM2_IwSOkoFDRvD6uKiokLQWt24oBNBUILlT1-IbM_heOyAyAsZKDYMfkJKUVAG1EQ9-RYypZDY1qZmRxFY0figHHbTShCH7jXSp6k1Lh8x5sRJPQ5aj4--fp1--0G0YbMF_vccCcMMtgthnovcUz8rYzIeH7w3lK7r58vp3flIsf11_nnxal5a1UpVMtwLLmSlALvFOW5lmUpDUqq4ApxLZjDbOtBSOZq5dOMLCOMoeSSgr8lFzs393E9cOIaat7nyyGYAZcj0lL4BwaWWdQ7UEb1ylF7PQm-t7EnaagJ5N6pSdhehKmJ5P6n0n9mFvPD3-Myx7dS-NBXQY-HACTrAldNIP16YVTDXAGKnOXe-6nD7h79QD62_f5FPFnFl6QBw</recordid><startdate>200704</startdate><enddate>200704</enddate><creator>Barceló‐Coblijn, Gwendolyn</creator><creator>Golovko, Mikhail Y.</creator><creator>Weinhofer, Isabella</creator><creator>Berger, Johannes</creator><creator>Murphy, Eric J.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200704</creationdate><title>Brain neutral lipids mass is increased in α‐synuclein gene‐ablated mice</title><author>Barceló‐Coblijn, Gwendolyn ; Golovko, Mikhail Y. ; Weinhofer, Isabella ; Berger, Johannes ; Murphy, Eric J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3978-d8900b53841c03f8c10688715e8c8028ee9f262c9c0a72d5bd420cd12de717103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>alpha-Synuclein - genetics</topic><topic>Animals</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain - metabolism</topic><topic>Brain Chemistry - genetics</topic><topic>Cardiolipins - metabolism</topic><topic>Central nervous system</topic><topic>cholesterol</topic><topic>Cholesterol - metabolism</topic><topic>cholesteryl esters</topic><topic>Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Enzymologic - physiology</topic><topic>Lipid Metabolism - genetics</topic><topic>Medical sciences</topic><topic>Membrane Lipids - metabolism</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Myelin Sheath - metabolism</topic><topic>Neurology</topic><topic>Phosphatidylglycerols - metabolism</topic><topic>Phospholipids - metabolism</topic><topic>triacylglycerols</topic><topic>Triglycerides - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><topic>α‐synuclein</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barceló‐Coblijn, Gwendolyn</creatorcontrib><creatorcontrib>Golovko, Mikhail Y.</creatorcontrib><creatorcontrib>Weinhofer, Isabella</creatorcontrib><creatorcontrib>Berger, Johannes</creatorcontrib><creatorcontrib>Murphy, Eric J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barceló‐Coblijn, Gwendolyn</au><au>Golovko, Mikhail Y.</au><au>Weinhofer, Isabella</au><au>Berger, Johannes</au><au>Murphy, Eric J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Brain neutral lipids mass is increased in α‐synuclein gene‐ablated mice</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>2007-04</date><risdate>2007</risdate><volume>101</volume><issue>1</issue><spage>132</spage><epage>141</epage><pages>132-141</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>Because α‐synuclein (Snca) has a role in brain lipid metabolism, we determined the impact that Snca deletion had on whole brain lipid composition. We analysed masses of individual phospholipid (PL) classes and neutral lipid mass as well as PL acyl chain composition in brains from wild‐type and Snca‐/‐ mice. Although total brain PL mass was not altered, cardiolipin and phosphatidylglycerol mass decreased 16% and 27%, respectively, in Snca‐/‐ mice. In addition, no changes were observed in plasmalogen or polyphosphoinositide mass. In ethanolamine glycerophospholipids and phosphatidylserine, docosahexaenoic acid (22 : 6n‐3) was decreased 7%, while 16 : 0 was increased 1.1‐fold and 1.4‐fold, respectively. Surprisingly, brain cholesterol, cholesteryl ester, and triacylglycerol mass were increased 1.1‐fold, 1.6‐fold, and 1.4‐fold, respectively in Snca‐/‐ mice. In isolated myelin, cholesterol mass was also increased 1.3‐fold, but because there was also a net increase in myelin PL mass, the cholesterol to PL ratio was unaltered. No changes in the expression of cholesterogenic enzymes were observed, suggesting these did not account for the observed changes in cholesterol. These data extend our previous results in astrocytes and kinetic studies in vivo demonstrating a role for Snca in brain lipid metabolism and demonstrate a clear impact on brain neutral lipid metabolism.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17250686</pmid><doi>10.1111/j.1471-4159.2006.04348.x</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3042 |
| ispartof | Journal of neurochemistry, 2007-04, Vol.101 (1), p.132-141 |
| issn | 0022-3042 1471-4159 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_70330675 |
| source | MEDLINE; Wiley Journals; IngentaConnect Free/Open Access Journals; EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); Free Full-Text Journals in Chemistry |
| subjects | alpha-Synuclein - genetics Animals Biochemistry and metabolism Biological and medical sciences Brain - metabolism Brain Chemistry - genetics Cardiolipins - metabolism Central nervous system cholesterol Cholesterol - metabolism cholesteryl esters Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Enzymologic - physiology Lipid Metabolism - genetics Medical sciences Membrane Lipids - metabolism Mice Mice, Knockout Myelin Sheath - metabolism Neurology Phosphatidylglycerols - metabolism Phospholipids - metabolism triacylglycerols Triglycerides - metabolism Vertebrates: nervous system and sense organs α‐synuclein |
| title | Brain neutral lipids mass is increased in α‐synuclein gene‐ablated mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T02%3A09%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Brain%20neutral%20lipids%20mass%20is%20increased%20in%20%CE%B1%E2%80%90synuclein%20gene%E2%80%90ablated%20mice&rft.jtitle=Journal%20of%20neurochemistry&rft.au=Barcel%C3%B3%E2%80%90Coblijn,%20Gwendolyn&rft.date=2007-04&rft.volume=101&rft.issue=1&rft.spage=132&rft.epage=141&rft.pages=132-141&rft.issn=0022-3042&rft.eissn=1471-4159&rft.coden=JONRA9&rft_id=info:doi/10.1111/j.1471-4159.2006.04348.x&rft_dat=%3Cproquest_cross%3E70330675%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70330675&rft_id=info:pmid/17250686&rfr_iscdi=true |