Development of a rapid immunochromatographic test for noroviruses genogroups I and II
Norovirus (NoV) is known to cause acute gastroenteritis in children worldwide. Although reverse transcription-PCR (RT-PCR) method is considered to be the “gold standard” for diagnosis of this viral infection, it requires skillful personnel and well-equipped laboratory. In this study, a rapid and eas...
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| Veröffentlicht in: | Journal of virological methods 2008-03, Vol.148 (1), p.1-8 |
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| creator | Takanashi, Sayaka Okame, Michio Shiota, Tomoyuki Takagi, Makiko Yagyu, Fumihiro Tung, Phan Gia Nishimura, Syuichi Katsumata, Noriko Igarashi, Takashi Okitsu, Shoko Ushijima, Hiroshi |
| description | Norovirus (NoV) is known to cause acute gastroenteritis in children worldwide. Although reverse transcription-PCR (RT-PCR) method is considered to be the “gold standard” for diagnosis of this viral infection, it requires skillful personnel and well-equipped laboratory. In this study, a rapid and easily performable diagnostic kit was developed using immunochromatographic method with rabbit polyclonal antibodies raised against recombinant virus-like particles (rVLPs) of most prevalent genotypes, genogroup II genotypes 3 and 4. This kit was evaluated for reactivity to rVLPs and detection of natural viruses in stool samples collected from children with diarrhea in comparison to the results obtained by RT-PCR. In the prospective assessment, the kit showed agreement rate of 84.1%, sensitivity of 69.8% and specificity of 93.7%. Genotyping of the RT-PCR positive samples by sequence analysis revealed that some heterogeneous genotypes were also detected while some in homogeneous genotypes occasionally showed false negative records resulting in lower sensitivity. No cross-reactivity with other common viral pathogens was observed. Taken together with the result of the detection limit of viral load as small as approximately 10
6–7
copies/g of stool, the current immunochromatography test is justified for screening for NoV infection with simple laboratory support. |
| doi_str_mv | 10.1016/j.jviromet.2007.10.010 |
| format | Article |
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6–7
copies/g of stool, the current immunochromatography test is justified for screening for NoV infection with simple laboratory support.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2007.10.010</identifier><identifier>PMID: 18054091</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Antibodies, Viral ; Biological and medical sciences ; Caliciviridae Infections - diagnosis ; Caliciviridae Infections - virology ; Child ; Chromatography - methods ; Cross Reactions ; False Negative Reactions ; Feces - virology ; Fundamental and applied biological sciences. Psychology ; Gastroenteritis - diagnosis ; Gastroenteritis - virology ; Genotype ; Humans ; Immunochromatography ; Microbiology ; Norovirus ; Norovirus - classification ; Norovirus - genetics ; Norovirus - immunology ; Norovirus - isolation & purification ; Phylogeny ; Rabbits ; Rapid detection test ; Recombinant virus-like particles ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Viral - genetics ; Sensitivity and Specificity ; Sequence Analysis, DNA ; Techniques used in virology ; Virology ; Virosomes - immunology</subject><ispartof>Journal of virological methods, 2008-03, Vol.148 (1), p.1-8</ispartof><rights>2007 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-b45a37b2949147df447efcfcb8843d64639e32cb06bdc09f71b1c743263b5b153</citedby><cites>FETCH-LOGICAL-c493t-b45a37b2949147df447efcfcb8843d64639e32cb06bdc09f71b1c743263b5b153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jviromet.2007.10.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20144485$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18054091$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takanashi, Sayaka</creatorcontrib><creatorcontrib>Okame, Michio</creatorcontrib><creatorcontrib>Shiota, Tomoyuki</creatorcontrib><creatorcontrib>Takagi, Makiko</creatorcontrib><creatorcontrib>Yagyu, Fumihiro</creatorcontrib><creatorcontrib>Tung, Phan Gia</creatorcontrib><creatorcontrib>Nishimura, Syuichi</creatorcontrib><creatorcontrib>Katsumata, Noriko</creatorcontrib><creatorcontrib>Igarashi, Takashi</creatorcontrib><creatorcontrib>Okitsu, Shoko</creatorcontrib><creatorcontrib>Ushijima, Hiroshi</creatorcontrib><title>Development of a rapid immunochromatographic test for noroviruses genogroups I and II</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>Norovirus (NoV) is known to cause acute gastroenteritis in children worldwide. Although reverse transcription-PCR (RT-PCR) method is considered to be the “gold standard” for diagnosis of this viral infection, it requires skillful personnel and well-equipped laboratory. In this study, a rapid and easily performable diagnostic kit was developed using immunochromatographic method with rabbit polyclonal antibodies raised against recombinant virus-like particles (rVLPs) of most prevalent genotypes, genogroup II genotypes 3 and 4. This kit was evaluated for reactivity to rVLPs and detection of natural viruses in stool samples collected from children with diarrhea in comparison to the results obtained by RT-PCR. In the prospective assessment, the kit showed agreement rate of 84.1%, sensitivity of 69.8% and specificity of 93.7%. Genotyping of the RT-PCR positive samples by sequence analysis revealed that some heterogeneous genotypes were also detected while some in homogeneous genotypes occasionally showed false negative records resulting in lower sensitivity. No cross-reactivity with other common viral pathogens was observed. Taken together with the result of the detection limit of viral load as small as approximately 10
6–7
copies/g of stool, the current immunochromatography test is justified for screening for NoV infection with simple laboratory support.</description><subject>Animals</subject><subject>Antibodies, Viral</subject><subject>Biological and medical sciences</subject><subject>Caliciviridae Infections - diagnosis</subject><subject>Caliciviridae Infections - virology</subject><subject>Child</subject><subject>Chromatography - methods</subject><subject>Cross Reactions</subject><subject>False Negative Reactions</subject><subject>Feces - virology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastroenteritis - diagnosis</subject><subject>Gastroenteritis - virology</subject><subject>Genotype</subject><subject>Humans</subject><subject>Immunochromatography</subject><subject>Microbiology</subject><subject>Norovirus</subject><subject>Norovirus - classification</subject><subject>Norovirus - genetics</subject><subject>Norovirus - immunology</subject><subject>Norovirus - isolation & purification</subject><subject>Phylogeny</subject><subject>Rabbits</subject><subject>Rapid detection test</subject><subject>Recombinant virus-like particles</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Viral - genetics</subject><subject>Sensitivity and Specificity</subject><subject>Sequence Analysis, DNA</subject><subject>Techniques used in virology</subject><subject>Virology</subject><subject>Virosomes - immunology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1v2yAUhlG1qsna_oWKm-0u2cFgY99tavcRqdJummsE-JAS2cYFO9L-_YiSbpe5Ar16Du_hIeSBwZoBq77s1_uDj6HHaV0AyByugcEVWbJaNitoavGBLDNY5TsXC_IxpT0AlJLzG7JgNZQCGrYk2yc8YBfGHoeJBkc1jXr0LfV9Pw_BvuYKPYVdDl-9pROmiboQ6RBiyP1zwkR3OGQgzGOiG6qHlm42d-Ta6S7h_fm8Jdsf318ef62ef__cPH57XlnR8GllRKm5NEUjGiZk64SQ6Kyzpq4FbytR8QZ5YQ1UprXQOMkMs1LwouKmNKzkt-Tz6d0xhrc5L6d6nyx2nR4wzElJ4IWEor4IFiBlWcsqg9UJtDGkFNGpMfpexz-KgTqaV3v1bl4dzR_zbD4PPpwbZtNj-3_srDoDn86ATlZ3LurB-vSPK4AJIerjn76eOMziDh6jStbjYLH1Ee2k2uAv7fIXJoallg</recordid><startdate>20080301</startdate><enddate>20080301</enddate><creator>Takanashi, Sayaka</creator><creator>Okame, Michio</creator><creator>Shiota, Tomoyuki</creator><creator>Takagi, Makiko</creator><creator>Yagyu, Fumihiro</creator><creator>Tung, Phan Gia</creator><creator>Nishimura, Syuichi</creator><creator>Katsumata, Noriko</creator><creator>Igarashi, Takashi</creator><creator>Okitsu, Shoko</creator><creator>Ushijima, Hiroshi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20080301</creationdate><title>Development of a rapid immunochromatographic test for noroviruses genogroups I and II</title><author>Takanashi, Sayaka ; Okame, Michio ; Shiota, Tomoyuki ; Takagi, Makiko ; Yagyu, Fumihiro ; Tung, Phan Gia ; Nishimura, Syuichi ; Katsumata, Noriko ; Igarashi, Takashi ; Okitsu, Shoko ; Ushijima, Hiroshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-b45a37b2949147df447efcfcb8843d64639e32cb06bdc09f71b1c743263b5b153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antibodies, Viral</topic><topic>Biological and medical sciences</topic><topic>Caliciviridae Infections - diagnosis</topic><topic>Caliciviridae Infections - virology</topic><topic>Child</topic><topic>Chromatography - methods</topic><topic>Cross Reactions</topic><topic>False Negative Reactions</topic><topic>Feces - virology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastroenteritis - diagnosis</topic><topic>Gastroenteritis - virology</topic><topic>Genotype</topic><topic>Humans</topic><topic>Immunochromatography</topic><topic>Microbiology</topic><topic>Norovirus</topic><topic>Norovirus - classification</topic><topic>Norovirus - genetics</topic><topic>Norovirus - immunology</topic><topic>Norovirus - isolation & purification</topic><topic>Phylogeny</topic><topic>Rabbits</topic><topic>Rapid detection test</topic><topic>Recombinant virus-like particles</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Viral - genetics</topic><topic>Sensitivity and Specificity</topic><topic>Sequence Analysis, DNA</topic><topic>Techniques used in virology</topic><topic>Virology</topic><topic>Virosomes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takanashi, Sayaka</creatorcontrib><creatorcontrib>Okame, Michio</creatorcontrib><creatorcontrib>Shiota, Tomoyuki</creatorcontrib><creatorcontrib>Takagi, Makiko</creatorcontrib><creatorcontrib>Yagyu, Fumihiro</creatorcontrib><creatorcontrib>Tung, Phan Gia</creatorcontrib><creatorcontrib>Nishimura, Syuichi</creatorcontrib><creatorcontrib>Katsumata, Noriko</creatorcontrib><creatorcontrib>Igarashi, Takashi</creatorcontrib><creatorcontrib>Okitsu, Shoko</creatorcontrib><creatorcontrib>Ushijima, Hiroshi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takanashi, Sayaka</au><au>Okame, Michio</au><au>Shiota, Tomoyuki</au><au>Takagi, Makiko</au><au>Yagyu, Fumihiro</au><au>Tung, Phan Gia</au><au>Nishimura, Syuichi</au><au>Katsumata, Noriko</au><au>Igarashi, Takashi</au><au>Okitsu, Shoko</au><au>Ushijima, Hiroshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a rapid immunochromatographic test for noroviruses genogroups I and II</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2008-03-01</date><risdate>2008</risdate><volume>148</volume><issue>1</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>Norovirus (NoV) is known to cause acute gastroenteritis in children worldwide. Although reverse transcription-PCR (RT-PCR) method is considered to be the “gold standard” for diagnosis of this viral infection, it requires skillful personnel and well-equipped laboratory. In this study, a rapid and easily performable diagnostic kit was developed using immunochromatographic method with rabbit polyclonal antibodies raised against recombinant virus-like particles (rVLPs) of most prevalent genotypes, genogroup II genotypes 3 and 4. This kit was evaluated for reactivity to rVLPs and detection of natural viruses in stool samples collected from children with diarrhea in comparison to the results obtained by RT-PCR. In the prospective assessment, the kit showed agreement rate of 84.1%, sensitivity of 69.8% and specificity of 93.7%. Genotyping of the RT-PCR positive samples by sequence analysis revealed that some heterogeneous genotypes were also detected while some in homogeneous genotypes occasionally showed false negative records resulting in lower sensitivity. No cross-reactivity with other common viral pathogens was observed. Taken together with the result of the detection limit of viral load as small as approximately 10
6–7
copies/g of stool, the current immunochromatography test is justified for screening for NoV infection with simple laboratory support.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>18054091</pmid><doi>10.1016/j.jviromet.2007.10.010</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antibodies, Viral Biological and medical sciences Caliciviridae Infections - diagnosis Caliciviridae Infections - virology Child Chromatography - methods Cross Reactions False Negative Reactions Feces - virology Fundamental and applied biological sciences. Psychology Gastroenteritis - diagnosis Gastroenteritis - virology Genotype Humans Immunochromatography Microbiology Norovirus Norovirus - classification Norovirus - genetics Norovirus - immunology Norovirus - isolation & purification Phylogeny Rabbits Rapid detection test Recombinant virus-like particles Reverse Transcriptase Polymerase Chain Reaction RNA, Viral - genetics Sensitivity and Specificity Sequence Analysis, DNA Techniques used in virology Virology Virosomes - immunology |
| title | Development of a rapid immunochromatographic test for noroviruses genogroups I and II |
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